PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

GRMZM2G124524_P01

Common Name

EREB85, LOC100217237

Organism

Zea mays

Taxonomic ID

4577

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea

Family

AP2

Protein Properties

Length: 395aa MW: 43488 Da PI: 4.5992

Description

AP2 family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
GRMZM2G124524_P01	genome	MaizeSequence	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	49.3	1.2e-15	63	123	1	55
AP2 1 sgykGVrwdkkrgrWvAeIrd.pse...ng..krkrfslgkfgtaeeAakaaiaarkkleg 55 s y+GV++++++gr++A+++d + + k ++++lg ++++e Aa+a++ a++k++g GRMZM2G124524_P01 63 SVYRGVTRHRWTGRFEAHLWDkHCLaalHNkkKGRQVYLGAYDSEEAAARAYDLAALKYWG 123 78*****************9999888445555***********************98 PP
2	AP2	56.5	6.8e-18	166	217	1	55
AP2 1 sgykGVrwdkkrgrWvAeIrd.psengkrkrfslgkfgtaeeAakaaiaarkkleg 55 s+y+GV +++ +grW+A+I + g +k+ +lg+f+t eeAaka++ a+ +++g GRMZM2G124524_P01 166 SKYRGVARHHHNGRWEARIGRvF---G-NKYLYLGTFDTQEEAAKAYDLAAIEYRG 217 89**99******99966...3.6***********************97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF54171	3.79E-17	63	132	IPR016177	DNA-binding domain
CDD	cd00018	8.44E-11	63	132	No hit	No description
Pfam	PF00847	6.2E-13	63	123	IPR001471	AP2/ERF domain
SMART	SM00380	1.1E-27	64	137	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	20.6	64	131	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	1.3E-16	64	132	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	2.55E-18	166	227	IPR016177	DNA-binding domain
CDD	cd00018	1.70E-25	166	227	No hit	No description
Pfam	PF00847	2.2E-13	166	217	IPR001471	AP2/ERF domain
SMART	SM00380	1.0E-26	167	231	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	2.2E-18	167	225	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	19.019	167	225	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006110	Biological Process	regulation of glycolytic process
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009744	Biological Process	response to sucrose
GO:0019432	Biological Process	triglyceride biosynthetic process
GO:1901959	Biological Process	positive regulation of cutin biosynthetic process
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0006339	anatomy	juvenile vascular leaf
PO:0008018	anatomy	transition vascular leaf
PO:0009001	anatomy	fruit
PO:0009009	anatomy	plant embryo
PO:0009025	anatomy	vascular leaf
PO:0025142	anatomy	leaf tip
PO:0001052	developmental stage	vascular leaf expansion stage
PO:0001095	developmental stage	plant embryo true leaf formation stage
PO:0007001	developmental stage	early whole plant fruit ripening stage
PO:0007022	developmental stage	seed imbibition stage
PO:0007031	developmental stage	mid whole plant fruit ripening stage
PO:0007063	developmental stage	LP.07 seven leaves visible stage

Sequence ? help Back to Top
Protein Sequence Length: 395 aa Download sequence Send to blast
MERSQRQSPP PPSPSSSSSS VSADTVLVPP GKRRRAATAK AGAEPNKRIR KDPAAAAAGK 60 RSSVYRGVTR HRWTGRFEAH LWDKHCLAAL HNKKKGRQVY LGAYDSEEAA ARAYDLAALK 120 YWGPETLLNF PVEDYSSEMP EMEAVSREEY LASLRRRSSG FSRGVSKYRG VARHHHNGRW 180 EARIGRVFGN KYLYLGTFDT QEEAAKAYDL AAIEYRGVNA VTNFDISCYL DHPLFLAQLQ 240 QEPQVVPALN QEPQPDQSET GTTEQEPESS EAKTPDGSAE PDENAVPDDT AEPLTTVDDS 300 IEEGLWSPCM DYELDTMSRP NFGSSINLSE WFADADFDCN IGCLFDGCSA ADEGSKDGVG 360 LADFSLFEAG DVQLKDVLSD MEEGIQPPAM ISVCN

Sequence ? help Back to Top

Protein Sequence Length: 395 aa Download sequence Send to blast

MERSQRQSPP PPSPSSSSSS VSADTVLVPP GKRRRAATAK AGAEPNKRIR KDPAAAAAGK  60
RSSVYRGVTR HRWTGRFEAH LWDKHCLAAL HNKKKGRQVY LGAYDSEEAA ARAYDLAALK  120
YWGPETLLNF PVEDYSSEMP EMEAVSREEY LASLRRRSSG FSRGVSKYRG VARHHHNGRW  180
EARIGRVFGN KYLYLGTFDT QEEAAKAYDL AAIEYRGVNA VTNFDISCYL DHPLFLAQLQ  240
QEPQVVPALN QEPQPDQSET GTTEQEPESS EAKTPDGSAE PDENAVPDDT AEPLTTVDDS  300
IEEGLWSPCM DYELDTMSRP NFGSSINLSE WFADADFDCN IGCLFDGCSA ADEGSKDGVG  360
LADFSLFEAG DVQLKDVLSD MEEGIQPPAM ISVCN

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Zm.8972	0.0	embryo\| endosperm\| ovary\| root

Expression -- Microarray ? help Back to Top
Source	ID
Expression Atlas	GRMZM2G124524

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Mostly expressed in siliques, especially in seeds. Also detected in roots and flowers, and, to a lower extent, in leaves stems and seedlings. {ECO:0000269\|PubMed:15500472, ECO:0000269\|PubMed:15753106}.

Functional Description ? help Back to Top
Source	Description
UniProt	May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds. Binds to the GCC-box pathogenesis-related promoter element. Promotes sugar uptake and seed oil accumulation by glycolysis. Required for embryo development, seed germination and, indirectly, for seedling establishment. Negative regulator of the ABA-mediated germination inhibition. {ECO:0000250, ECO:0000269\|PubMed:12084821, ECO:0000269\|PubMed:15500472, ECO:0000269\|PubMed:15753106, ECO:0000269\|PubMed:16553903, ECO:0000269\|PubMed:16632590, ECO:0000269\|PubMed:9733529}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	GRMZM2G124524_P01

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Transiantly in leaves by sucrose, but not by abscisic acid (ABA). {ECO:0000269\|PubMed:15753106}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	BT038184	0.0	BT038184.1 Zea mays full-length cDNA clone ZM_BFb0216H15 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001137064.1	0.0	uncharacterized protein LOC100217237
Swissprot	Q6X5Y6	4e-94	WRI1_ARATH; Ethylene-responsive transcription factor WRI1
TrEMBL	B4FM46	0.0	B4FM46_MAIZE; AP2-EREBP transcription factor
STRING	GRMZM2G124524_P01	0.0	(Zea mays)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP5526	35	57
Representative plant	OGRP112	17	209

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G54320.3	2e-77	AP2 family protein

Link Out ? help Back to Top
Phytozome	GRMZM2G124524_P01
Entrez Gene	100217237

Publications ? help Back to Top
Alexandrov NN, et al. Insights into corn genes derived from large-scale cDNA sequencing. Plant Mol. Biol., 2009. 69(1-2): p. 179-94 [PMID:18937034] Kim HU, et al. Ectopic overexpression of castor bean LEAFY COTYLEDON2 (LEC2) in Arabidopsis triggers the expression of genes that encode regulators of seed maturation and oil body proteins in vegetative tissues. FEBS Open Bio, 2013. 4: p. 25-32 [PMID:24363987] Wu XL,Liu ZH,Hu ZH,Huang RZ BnWRI1 coordinates fatty acid biosynthesis and photosynthesis pathways during oil accumulation in rapeseed. J Integr Plant Biol, 2014. 56(6): p. 582-93 [PMID:24393360] Shigematsu H, et al. Structural characterization of the mechanosensitive channel candidate MCA2 from Arabidopsis thaliana. PLoS ONE, 2014. 9(1): p. e87724 [PMID:24475319] Kim HU, et al. Senescence-inducible LEC2 enhances triacylglycerol accumulation in leaves without negatively affecting plant growth. Plant Biotechnol. J., 2015. 13(9): p. 1346-59 [PMID:25790072] Grimberg Å,Carlsson AS,Marttila S,Bhalerao R,Hofvander P Transcriptional transitions in Nicotiana benthamiana leaves upon induction of oil synthesis by WRINKLED1 homologs from diverse species and tissues. BMC Plant Biol., 2015. 15: p. 192 [PMID:26253704] Kanai M,Mano S,Kondo M,Hayashi M,Nishimura M Extension of oil biosynthesis during the mid-phase of seed development enhances oil content in Arabidopsis seeds. Plant Biotechnol. J., 2016. 14(5): p. 1241-50 [PMID:26503031] Li Q, et al. Wrinkled1 Accelerates Flowering and Regulates Lipid Homeostasis between Oil Accumulation and Membrane Lipid Anabolism in Brassica napus. Front Plant Sci, 2015. 6: p. 1015 [PMID:26635841] Hofvander P, et al. Potato tuber expression of Arabidopsis WRINKLED1 increase triacylglycerol and membrane lipids while affecting central carbohydrate metabolism. Plant Biotechnol. J., 2016. 14(9): p. 1883-98 [PMID:26914183] Shen SL, et al. CitAP2.10 activation of the terpene synthase CsTPS1 is associated with the synthesis of (+)-valencene in 'Newhall' orange. J. Exp. Bot., 2016. 67(14): p. 4105-15 [PMID:27194737] Adhikari ND,Bates PD,Browse J WRINKLED1 Rescues Feedback Inhibition of Fatty Acid Synthesis in Hydroxylase-Expressing Seeds. Plant Physiol., 2016. 171(1): p. 179-91 [PMID:27208047] Kim MJ,Jang IC,Chua NH The Mediator Complex MED15 Subunit Mediates Activation of Downstream Lipid-Related Genes by the WRINKLED1 Transcription Factor. Plant Physiol., 2016. 171(3): p. 1951-64 [PMID:27246098] Bhattacharya S,Das N,Maiti MK Cumulative effect of heterologous AtWRI1 gene expression and endogenous BjAGPase gene silencing increases seed lipid content in Indian mustard Brassica juncea. Plant Physiol. Biochem., 2016. 107: p. 204-213 [PMID:27314514] Ma W, et al. 14-3-3 protein mediates plant seed oil biosynthesis through interaction with AtWRI1. Plant J., 2016. 88(2): p. 228-235 [PMID:27322486] Li D, et al. MYB89 Transcription Factor Represses Seed Oil Accumulation. Plant Physiol., 2017. 173(2): p. 1211-1225 [PMID:27932421] Ivarson E, et al. Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre. Front Plant Sci, 2016. 7: p. 2032 [PMID:28119714] An D, et al. Expression of Camelina WRINKLED1 Isoforms Rescue the Seed Phenotype of the Arabidopsis wri1 Mutant and Increase the Triacylglycerol Content in Tobacco Leaves. Front Plant Sci, 2017. 8: p. 34 [PMID:28174580] Zhai Z,Liu H,Shanklin J Phosphorylation of WRINKLED1 by KIN10 Results in Its Proteasomal Degradation, Providing a Link between Energy Homeostasis and Lipid Biosynthesis. Plant Cell, 2017. 29(4): p. 871-889 [PMID:28314829] Jin J, et al. Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm. Sci Rep, 2017. 7(1): p. 439 [PMID:28348403] Kong Q, et al. The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots. J. Exp. Bot., 2017. 68(16): p. 4627-4634 [PMID:28981783] Kang NK, et al. Increased lipid production by heterologous expression of AtWRI1 transcription factor in Nannochloropsis salina. Biotechnol Biofuels, 2017. 10: p. 231 [PMID:29046718] Pellaud S, et al. WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis. New Phytol., 2018. 217(1): p. 245-260 [PMID:29105089] Hanano A,Almousally I,Shaban M,Murphy DJ Arabidopsis plants exposed to dioxin result in a WRINKLED seed phenotype due to 20S proteasomal degradation of WRI1. J. Exp. Bot., 2018. 69(7): p. 1781-1794 [PMID:29394403]