PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Home  TFext  BLAST  Prediction  Download  Help  About  Links  PlantRegMap
(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID GRMZM2G033356_P01
Common NameZEAMMB73_380614, Zm.5259
Organism
Zea mays
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea
Family bHLH
Protein Properties Length: 518aa    MW: 53267.9 Da    PI: 4.8433
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
GRMZM2G033356_P01genomeMaizeSequenceView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH35.91.3e-11331373754
                        HHHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHHHH CS
                HLH   7 erErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksL 54 
                        ++ErrRR+++N+++  Lr+++Pk +      K++ a+iL  A+eY+k+L
  GRMZM2G033356_P01 331 MAERRRRKKLNDRLYMLRSVVPKiS------KMDRASILGDAIEYLKEL 373
                        79*********************66......****************98 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5088815.58324373IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474591.83E-16327398IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003534.4E-14330379IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000103.1E-9330373IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000834.74E-13331377No hitNo description
Gene3DG3DSA:4.10.280.106.7E-16331386IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd048739.26E-4449486No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0010440Biological Processstomatal lineage progression
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0050826Biological Processresponse to freezing
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0006310anatomytassel floret
PO:0006339anatomyjuvenile vascular leaf
PO:0006340anatomyadult vascular leaf
PO:0006505anatomycentral spike of ear inflorescence
PO:0008018anatomytransition vascular leaf
PO:0009001anatomyfruit
PO:0009025anatomyvascular leaf
PO:0009054anatomyinflorescence bract
PO:0009066anatomyanther
PO:0009084anatomypericarp
PO:0009089anatomyendosperm
PO:0020040anatomyleaf base
PO:0020126anatomytassel inflorescence
PO:0020127anatomyprimary root
PO:0020136anatomyear inflorescence
PO:0020142anatomystem internode
PO:0020148anatomyshoot apical meristem
PO:0025142anatomyleaf tip
PO:0001007developmental stagepollen development stage
PO:0001009developmental stageD pollen mother cell meiosis stage
PO:0001052developmental stagevascular leaf expansion stage
PO:0001053developmental stagevascular leaf post-expansion stage
PO:0001083developmental stageinflorescence development stage
PO:0001094developmental stageplant embryo coleoptilar stage
PO:0001095developmental stageplant embryo true leaf formation stage
PO:0001180developmental stageplant proembryo stage
PO:0007001developmental stageearly whole plant fruit ripening stage
PO:0007003developmental stageIL.03 full inflorescence length reached stage
PO:0007006developmental stageIL.00 inflorescence just visible stage
PO:0007016developmental stagewhole plant flowering stage
PO:0007022developmental stageseed imbibition stage
PO:0007026developmental stageFL.00 first flower(s) open stage
PO:0007031developmental stagemid whole plant fruit ripening stage
PO:0007032developmental stagewhole plant fruit formation stage up to 10%
PO:0007063developmental stageLP.07 seven leaves visible stage
PO:0007065developmental stageLP.05 five leaves visible stage
PO:0007072developmental stageLP.18 eighteen leaves visible stage
PO:0007101developmental stageLP.09 nine leaves visible stage
PO:0007104developmental stageLP.15 fifteen leaves visible stage
PO:0007106developmental stageLP.03 three leaves visible stage
PO:0007116developmental stageLP.11 eleven leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007633developmental stageendosperm development stage
PO:0021004developmental stageinflorescence initiation stage
Sequence ? help Back to Top
Protein Sequence    Length: 518 aa     Download sequence    Send to blast
MLPPFGNPLW VPEDVDDQQQ HHAPPPTPMG LGPGQGHDEQ NLLALASAAA MGAGGIFSSP  60
AVLDDDWYFD PVAAAAAAGA QGQLLLAPPG PAPDAGSQMF SLFNVGGAAT FDVHGFDLGL  120
GGGGGGDLVP FAGAGNASNS ASFSLVPAGN AGGFLGSFGG FGTAPAQMPD FGGLGGFDMF  180
NSGAGSSSAA PPPPASVSLT APFSGRGKAA VLRPLEIFPP VGAQPTLFQK RALRRNAGEE  240
DDDKKRKVEA VAAAAGASSG GGGDTVLDDA DDDDGGSIDA SGLNYDSEDA RGVEDSGKKD  300
GKDSNANSTV TGGATGDGKG KRKGLPAKNL MAERRRRKKL NDRLYMLRSV VPKISKMDRA  360
SILGDAIEYL KELLQKINDL QNDLESSPST ASLPPTPTSF HPLTPTLPTL PSRVKEELCP  420
SALPSPTSQQ PRVEVRMREG RAVNIHMLCA RRPGLLLSAM RAIEGLGLDV QQAVISCFNG  480
FSLDIFKAEL CKEGPGLLPE EIKSVLLQSA GFHGGVMP
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5gnj_A2e-14326377657Transcription factor MYC2
5gnj_B2e-14326377657Transcription factor MYC2
5gnj_E2e-14326377657Transcription factor MYC2
5gnj_F2e-14326377657Transcription factor MYC2
5gnj_G2e-14326377657Transcription factor MYC2
5gnj_I2e-14326377657Transcription factor MYC2
5gnj_M2e-14326377657Transcription factor MYC2
5gnj_N2e-14326377657Transcription factor MYC2
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1320338KRKGLPAKNLMAERRRRKK
2332339ERRRRKKL
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Zm.52590.0embryo| meristem| shoot
Expression -- Microarray ? help Back to Top
Source ID
Expression AtlasGRMZM2G033356
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Widely expressed in the whole plant with high expression in leaves and stem. Broad expression within stomatal cell lineages of leaf epidermis. {ECO:0000269|PubMed:18641265}.
Functional Description ? help Back to Top
Source Description
UniProtTranscriptional activator that regulates the cold-induced transcription of CBF/DREB1 genes. Binds specifically to the MYC recognition sites (5'-CANNTG-3') found in the CBF3/DREB1A promoter. Mediates stomatal differentiation in the epidermis probably by controlling successive roles of SPCH, MUTE, and FAMA. Functions as a dimer with SPCH during stomatal initiation (PubMed:18641265, PubMed:28507175). {ECO:0000269|PubMed:17416732, ECO:0000269|PubMed:18641265, ECO:0000269|PubMed:28507175}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapGRMZM2G033356_P01
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By high-salt stress, cold stress and abscisic acid (ABA) treatment.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_008678166.20.0transcription factor SCREAM2
SwissprotQ9LSE21e-113ICE1_ARATH; Transcription factor ICE1
TrEMBLK7UAI80.0K7UAI8_MAIZE; Putative HLH DNA-binding domain superfamily protein
STRINGGRMZM2G033356_P010.0(Zea mays)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MonocotsOGMP164637101
Representative plantOGRP5571678
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT1G12860.19e-87bHLH family protein
Publications ? help Back to Top
  1. Chen Y, et al.
    Ambient temperature enhanced freezing tolerance of Chrysanthemum dichrum CdICE1 Arabidopsis via miR398.
    BMC Biol., 2013. 11: p. 121
    [PMID:24350981]
  2. Xu F, et al.
    Increased drought tolerance through the suppression of ESKMO1 gene and overexpression of CBF-related genes in Arabidopsis.
    PLoS ONE, 2014. 9(9): p. e106509
    [PMID:25184213]
  3. Jiang W,Wu J,Zhang Y,Yin L,Lu J
    Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses.
    Protoplasma, 2015. 252(5): p. 1361-74
    [PMID:25643917]
  4. Lang Z,Zhu J
    OST1 phosphorylates ICE1 to enhance plant cold tolerance.
    Sci China Life Sci, 2015. 58(3): p. 317-8
    [PMID:25680856]
  5. Juan JX, et al.
    Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene.
    Genet. Mol. Res., 2015. 14(1): p. 597-608
    [PMID:25729995]
  6. Lee HG,Seo PJ
    The MYB96-HHP module integrates cold and abscisic acid signaling to activate the CBF-COR pathway in Arabidopsis.
    Plant J., 2015. 82(6): p. 962-77
    [PMID:25912720]
  7. Horst RJ, et al.
    Molecular Framework of a Regulatory Circuit Initiating Two-Dimensional Spatial Patterning of Stomatal Lineage.
    PLoS Genet., 2015. 11(7): p. e1005374
    [PMID:26203655]
  8. Lee JH,Jung JH,Park CM
    INDUCER OF CBF EXPRESSION 1 integrates cold signals into FLOWERING LOCUS C-mediated flowering pathways in Arabidopsis.
    Plant J., 2015. 84(1): p. 29-40
    [PMID:26248809]
  9. Wang CL,Zhang SC,Qi SD,Zheng CC,Wu CA
    Delayed germination of Arabidopsis seeds under chilling stress by overexpressing an abiotic stress inducible GhTPS11.
    Gene, 2016. 575(2 Pt 1): p. 206-12
    [PMID:26325072]
  10. Lee JH,Park CM
    Integration of photoperiod and cold temperature signals into flowering genetic pathways in Arabidopsis.
    Plant Signal Behav, 2015. 10(11): p. e1089373
    [PMID:26430754]
  11. Su F, et al.
    Burkholderia phytofirmans PsJN reduces impact of freezing temperatures on photosynthesis in Arabidopsis thaliana.
    Front Plant Sci, 2015. 6: p. 810
    [PMID:26483823]
  12. Klermund C, et al.
    LLM-Domain B-GATA Transcription Factors Promote Stomatal Development Downstream of Light Signaling Pathways in Arabidopsis thaliana Hypocotyls.
    Plant Cell, 2016. 28(3): p. 646-60
    [PMID:26917680]
  13. Chen L, et al.
    NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis.
    Development, 2016. 143(9): p. 1600-11
    [PMID:26989174]
  14. Lu X, et al.
    A novel Zea mays ssp. mexicana L. MYC-type ICE-like transcription factor gene ZmmICE1, enhances freezing tolerance in transgenic Arabidopsis thaliana.
    Plant Physiol. Biochem., 2017. 113: p. 78-88
    [PMID:28189052]
  15. Deng C,Ye H,Fan M,Pu T,Yan J
    The rice transcription factors OsICE confer enhanced cold tolerance in transgenic Arabidopsis.
    Plant Signal Behav, 2017. 12(5): p. e1316442
    [PMID:28414264]
  16. de Marcos A, et al.
    A Mutation in the bHLH Domain of the SPCH Transcription Factor Uncovers a BR-Dependent Mechanism for Stomatal Development.
    Plant Physiol., 2017. 174(2): p. 823-842
    [PMID:28507175]
  17. Kim SH, et al.
    Phosphorylation of the transcriptional repressor MYB15 by mitogen-activated protein kinase 6 is required for freezing tolerance in Arabidopsis.
    Nucleic Acids Res., 2017. 45(11): p. 6613-6627
    [PMID:28510716]
  18. Pal S, et al.
    TransDetect Identifies a New Regulatory Module Controlling Phosphate Accumulation.
    Plant Physiol., 2017. 175(2): p. 916-926
    [PMID:28827455]
  19. Zhao C, et al.
    MAP Kinase Cascades Regulate the Cold Response by Modulating ICE1 Protein Stability.
    Dev. Cell, 2017. 43(5): p. 618-629.e5
    [PMID:29056551]
  20. Li H, et al.
    MPK3- and MPK6-Mediated ICE1 Phosphorylation Negatively Regulates ICE1 Stability and Freezing Tolerance in Arabidopsis.
    Dev. Cell, 2017. 43(5): p. 630-642.e4
    [PMID:29056553]
  21. Lee JH,Jung JH,Park CM
    Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis.
    Plant Cell, 2017. 29(11): p. 2817-2830
    [PMID:29070509]
  22. Liu Y,Zhou J
    MAPping Kinase Regulation of ICE1 in Freezing Tolerance.
    Trends Plant Sci., 2018. 23(2): p. 91-93
    [PMID:29248419]
  23. Xie H, et al.
    Variation in ICE1 Methylation Primarily Determines Phenotypic Variation in Freezing Tolerance in Arabidopsis thaliana.
    Plant Cell Physiol., 2019. 60(1): p. 152-165
    [PMID:30295898]