PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Tp57577_TGAC_v2_mRNA38291

Organism

Trifolium pratense

Taxonomic ID

57577

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Trifolieae; Trifolium

Family

WRKY

Protein Properties

Length: 497aa MW: 54351 Da PI: 5.1885

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Tp57577_TGAC_v2_mRNA38291	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	95.9	2.7e-30	264	322	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 +Dg++WrKYGqK+ kg+++pr+YYrCt+a gCpv+k+v+r+aed +++++tYeg+H h+ Tp57577_TGAC_v2_mRNA38291 264 NDGCQWRKYGQKMAKGNPCPRAYYRCTMAmGCPVRKQVQRCAEDRTILITTYEGTHSHP 322 8**************************9**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	2.7E-33	248	324	IPR003657	WRKY domain
SuperFamily	SSF118290	8.63E-28	256	324	IPR003657	WRKY domain
PROSITE profile	PS50811	29.126	258	324	IPR003657	WRKY domain
SMART	SM00774	4.7E-36	263	323	IPR003657	WRKY domain
Pfam	PF03106	1.4E-26	264	322	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 497 aa Download sequence Send to blast
MLTFPMNLMT NWSDDNENDN DNDGDQRRIN RVIVDEVDFF SDRNDKSSSP DDDVSMKINN 60 EIYDPHCNLA ADHVNTGLQL LISNTGSDQS MVEDVSSINA EEKRAKTAQV EQLQEELRFK 120 NEENQKLKEM LNTMRSSHTN LQMRYVSLMQ QKQNQTEHDQ VVRGKSEEKG DSVVARKFTS 180 GTAEVDDQQV SNSCNSDEKT QTSTPQNKRK LETDPDSSEL VQAWDPIKRP ILNPPNAADQ 240 SAAEATMRKA RVSVRARSEA HMINDGCQWR KYGQKMAKGN PCPRAYYRCT MAMGCPVRKQ 300 VQRCAEDRTI LITTYEGTHS HPLPPAAVAM ASTTAAAATV LLSGSMSSAD GVINPNILAR 360 ILPNCSSSMA TLSASAPFPT VTLDLTRDAT DNNQNSHSQF QLGQPQNFGS GQLPQVIAQA 420 LYNTSKFSGL QLSQDVGGSS QLNHTQQASS LSAAITSDPN FTAALAAAIS SIIGASPPNN 480 NNNNNPNIIN STTYNQ*

Sequence ? help Back to Top

Protein Sequence Length: 497 aa Download sequence Send to blast

MLTFPMNLMT NWSDDNENDN DNDGDQRRIN RVIVDEVDFF SDRNDKSSSP DDDVSMKINN  60
EIYDPHCNLA ADHVNTGLQL LISNTGSDQS MVEDVSSINA EEKRAKTAQV EQLQEELRFK  120
NEENQKLKEM LNTMRSSHTN LQMRYVSLMQ QKQNQTEHDQ VVRGKSEEKG DSVVARKFTS  180
GTAEVDDQQV SNSCNSDEKT QTSTPQNKRK LETDPDSSEL VQAWDPIKRP ILNPPNAADQ  240
SAAEATMRKA RVSVRARSEA HMINDGCQWR KYGQKMAKGN PCPRAYYRCT MAMGCPVRKQ  300
VQRCAEDRTI LITTYEGTHS HPLPPAAVAM ASTTAAAATV LLSGSMSSAD GVINPNILAR  360
ILPNCSSSMA TLSASAPFPT VTLDLTRDAT DNNQNSHSQF QLGQPQNFGS GQLPQVIAQA  420
LYNTSKFSGL QLSQDVGGSS QLNHTQQASS LSAAITSDPN FTAALAAAIS SIIGASPPNN  480
NNNNNPNIIN STTYNQ*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	2e-23	250	326	1	76	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the control of processes related to senescence and pathogen defense (PubMed:12000796). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:12000796). Activates the transcription of the SIRK gene and represses its own expression and that of the WRKY42 genes (PubMed:12000796). Modulates phosphate homeostasis and Pi translocation by regulating PHO1 expression (PubMed:25733771). {ECO:0000269\|PubMed:12000796, ECO:0000269\|PubMed:25733771}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Tp57577_TGAC_v2_mRNA38291

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid, ethylene, jasmonic acid, pathogens, wounding and strongly during leaf senescence. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11722756}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AC159536	0.0	AC159536.21 Medicago truncatula chromosome 8 clone mth2-187n4, complete sequence.
GenBank	AC225537	0.0	AC225537.2 Medicago truncatula clone mth2-66l14, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_003610463.2	0.0	probable WRKY transcription factor 31
Swissprot	Q9C519	1e-125	WRKY6_ARATH; WRKY transcription factor 6
TrEMBL	A0A2K3MPK3	0.0	A0A2K3MPK3_TRIPR; WRKY transcription factor 6-like protein
STRING	AES75500	0.0	(Medicago truncatula)
STRING	AES92660	0.0	(Medicago truncatula)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF866	34	121

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G62300.1	1e-117	WRKY family protein

Link Out ? help Back to Top
Phytozome	Tp57577_TGAC_v2_mRNA38291

Publications ? help Back to Top
Zhou F, et al. High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression. Plant J., 2004. 39(6): p. 920-32 [PMID:15341634] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P An extracellular subtilase switch for immune priming in Arabidopsis. PLoS Pathog., 2013. 9(6): p. e1003445 [PMID:23818851] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Singh V,Roy S,Singh D,Nandi AK Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. J. Biosci., 2014. 39(1): p. 119-26 [PMID:24499796] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Chai J,Liu J,Zhou J,Xing D Mitogen-activated protein kinase 6 regulates NPR1 gene expression and activation during leaf senescence induced by salicylic acid. J. Exp. Bot., 2014. 65(22): p. 6513-28 [PMID:25210078] Vermeirssen V,De Clercq I,Van Parys T,Van Breusegem F,Van de Peer Y Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress. Plant Cell, 2014. 26(12): p. 4656-79 [PMID:25549671] Xiao D, et al. SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE Directly Interacts with the Cytoplasmic Domain of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE and Negatively Regulates Leaf Senescence in Arabidopsis. Plant Physiol., 2015. 169(2): p. 1275-91 [PMID:26304848] Bakshi M, et al. WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis. BMC Plant Biol., 2015. 15: p. 305 [PMID:26718529] Huang Y,Feng CZ,Ye Q,Wu WH,Chen YF Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development. PLoS Genet., 2016. 12(2): p. e1005833 [PMID:26829043] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P Correction: An Extracellular Subtilase Switch for Immune Priming in Arabidopsis. PLoS Pathog., 2016. 12(11): p. e1006003 [PMID:27806116] Li H, et al. BZR1 Positively Regulates Freezing Tolerance via CBF-Dependent and CBF-Independent Pathways in Arabidopsis. Mol Plant, 2017. 10(4): p. 545-559 [PMID:28089951] Stetter MG,Benz M,Ludewig U Increased root hair density by loss of WRKY6 in Arabidopsis thaliana. PeerJ, 2017. 5: p. e2891 [PMID:28149680] Zhou X, et al. StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato. J. Exp. Bot., 2017. 68(5): p. 1265-1281 [PMID:28338870] Bakshi M, et al. Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation. Front Microbiol, 2017. 8: p. 1262 [PMID:28747898] Zhang Y, et al. DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6. Plant Cell Rep., 2018. 37(7): p. 981-992 [PMID:29574486]