PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Tp57577_TGAC_v2_mRNA2494

Organism

Trifolium pratense

Taxonomic ID

57577

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Trifolieae; Trifolium

Family

bHLH

Protein Properties

Length: 497aa MW: 56218.4 Da PI: 4.6572

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Tp57577_TGAC_v2_mRNA2494	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	36.9	6.7e-12	310	353	7	55
HHHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHHHHH CS HLH 7 erErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksLq 55 +Er+RR+++N+++ +Lr+l+P + Kl+ a+iL A+e++k+Lq Tp57577_TGAC_v2_mRNA2494 310 VAERKRRKKLNDRLYNLRSLVPRiS------KLDRASILGDAIEFVKDLQ 353 68*******************66......****************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	8.7E-20	6	150	IPR025610	Transcription factor MYC/MYB N-terminal
CDD	cd00083	3.23E-13	302	357	No hit	No description
PROSITE profile	PS50888	15.294	303	352	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	8.64E-17	306	367	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	3.3E-15	309	358	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	1.6E-9	310	353	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	2.6E-16	310	366	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd04873	2.21E-4	449	492	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009555	Biological Process	pollen development
GO:0048657	Biological Process	anther wall tapetum cell differentiation
GO:0005634	Cellular Component	nucleus
GO:0000978	Molecular Function	RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 497 aa Download sequence Send to blast
MQNFTERLRP LVGFNGWDYI VYWKLSDDQR FLEWLGCCCS GSESNQNVGE EHLFPISSCR 60 DTMFSHPRTK SCDLLSQLST SIPTDSGIYA QALLTNQPNW LNYPNTLDPN TLQETINGTH 120 VLIPVPGGLV ELFVTKQVGE DQQVIDLITS QCIAMVDQEG MNHSFGFNSM SNMQLNQVLG 180 DENDGNKTLN LPHDISVMNF MQQFNYSYNQ QQNRIKNDEN FSEEYHGSFL HDLENNNILK 240 SNTEEENDNK SLMTDNNQYM FVDPLDSKKK QENGVGRSDS MSDCSDQNEE EDDGKYRRRS 300 GKGNQSKNLV AERKRRKKLN DRLYNLRSLV PRISKLDRAS ILGDAIEFVK DLQKQVKELQ 360 DELEENSDTG TESNCIVNGI NNNQYVGGVQ VQPENPNNNV GRAEHGFHVG NGFVSKQKQE 420 DVGTVTDKQT QQMEPQVEVA QIDGNEYFLK VFCEHRPGGF VKLMEALNTL GMDVVHATVT 480 SHKGLVSNVF KVEVNI*

Sequence ? help Back to Top

Protein Sequence Length: 497 aa Download sequence Send to blast

MQNFTERLRP LVGFNGWDYI VYWKLSDDQR FLEWLGCCCS GSESNQNVGE EHLFPISSCR  60
DTMFSHPRTK SCDLLSQLST SIPTDSGIYA QALLTNQPNW LNYPNTLDPN TLQETINGTH  120
VLIPVPGGLV ELFVTKQVGE DQQVIDLITS QCIAMVDQEG MNHSFGFNSM SNMQLNQVLG  180
DENDGNKTLN LPHDISVMNF MQQFNYSYNQ QQNRIKNDEN FSEEYHGSFL HDLENNNILK  240
SNTEEENDNK SLMTDNNQYM FVDPLDSKKK QENGVGRSDS MSDCSDQNEE EDDGKYRRRS  300
GKGNQSKNLV AERKRRKKLN DRLYNLRSLV PRISKLDRAS ILGDAIEFVK DLQKQVKELQ  360
DELEENSDTG TESNCIVNGI NNNQYVGGVQ VQPENPNNNV GRAEHGFHVG NGFVSKQKQE  420
DVGTVTDKQT QQMEPQVEVA QIDGNEYFLK VFCEHRPGGF VKLMEALNTL GMDVVHATVT  480
SHKGLVSNVF KVEVNI*

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	296	317	RRRSGKGNQSKNLVAERKRRKK
2	311	318	ERKRRKKL

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther (PubMed:12535353). Binds E-box regions in the AHL16/TEK promoter. {ECO:0000269\|PubMed:12535353, ECO:0000269\|PubMed:24804694}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Tp57577_TGAC_v2_mRNA2494

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_013470329.1	0.0	transcription factor ABORTED MICROSPORES
Swissprot	Q9ZVX2	1e-133	AMS_ARATH; Transcription factor ABORTED MICROSPORES
TrEMBL	A0A2K3MN83	0.0	A0A2K3MN83_TRIPR; Putative transcription factor ABORTED MICROSPORES-like protein
STRING	XP_004497179.1	0.0	(Cicer arietinum)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF4225	32	47

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G16910.1	1e-131	bHLH family protein

Link Out ? help Back to Top
Phytozome	Tp57577_TGAC_v2_mRNA2494

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Jiang J,Zhang Z,Cao J Pollen wall development: the associated enzymes and metabolic pathways. Plant Biol (Stuttg), 2013. 15(2): p. 249-63 [PMID:23252839] Xu J, et al. ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis. Plant Cell, 2014. 26(4): p. 1544-1556 [PMID:24781116] Qian H, et al. Trace concentrations of imazethapyr (IM) affect floral organs development and reproduction in Arabidopsis thaliana: IM-induced inhibition of key genes regulating anther and pollen biosynthesis. Ecotoxicology, 2015. 24(1): p. 163-71 [PMID:25348600] Xiong SX, et al. The transcription factors MS188 and AMS form a complex to activate the expression of CYP703A2 for sporopollenin biosynthesis in Arabidopsis thaliana. Plant J., 2016. 88(6): p. 936-946 [PMID:27460657] Ferguson AC, et al. Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis. New Phytol., 2017. 213(2): p. 778-790 [PMID:27787905] Lou Y, et al. Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for anther development in Arabidopsis thaliana. New Phytol., 2018. 217(1): p. 378-391 [PMID:28940573] Bi H,Fan W,Zhang P C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis. Front Plant Sci, 2017. 8: p. 1689 [PMID:29021807]