PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Thecc1EG006871t1

Common Name

TCM_006871

Organism

Theobroma cacao

Taxonomic ID

3641

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Byttnerioideae; Theobroma

Family

bHLH

Protein Properties

Length: 441aa MW: 48564.9 Da PI: 5.86

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Thecc1EG006871t1	genome	CGD	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	30.7	5.9e-10	248	295	4	55
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +h+ +Er RR++i +++ L++l+P + +k Ka +L + ++Y++sLq Thecc1EG006871t1 248 SHSLAERVRREKISERMKYLQDLVPGC----NKITGKAGMLDEIINYVQSLQ 295 8************************....999***************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
CDD	cd00083	1.70E-10	242	299	No hit	No description
SuperFamily	SSF47459	6.67E-17	242	313	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	15.618	244	294	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	3.6E-17	244	312	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	2.4E-7	248	295	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	2.3E-9	250	300	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006351	Biological Process	transcription, DNA-templated
GO:0009637	Biological Process	response to blue light
GO:0009911	Biological Process	positive regulation of flower development
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 441 aa Download sequence Send to blast
MNRALPEMLQ CSDMTVLERQ RARLKWQQEQ LQQQQLQQQE QQQSYFSELS GVFSSQPSHV 60 EGFQGGLMSG DSVLGDMVMT RQLKPDPGLE TAWPELVKVD MPGMGFGPCG YGNGPSFDMN 120 YAISRTSSCP PAVAAAVAGE VVEVKGKESV VSEKIGSAVG RESFKKRKVD KLQNLKVVAE 180 DDSKRIKACA EEGESKITGP NTNKSSSNNN NNKKESSTDT SKENSKVSEV QKPDYIHVRA 240 RRGQATDSHS LAERVRREKI SERMKYLQDL VPGCNKITGK AGMLDEIINY VQSLQRQVEF 300 LSMKLAAVNP RLDFNVENLF AKEVFPSCTT NFPTVGMSSE MANPPYLQVS PVQHVVSCCG 360 LEMGMNTPDM APRRTISAPV SIPDASFLDS SCFPQIQPSA TWDVELQNLY NVAFDQGRST 420 SFPSQPFTGS IEASNLKMEM *

Sequence ? help Back to Top

Protein Sequence Length: 441 aa Download sequence Send to blast

MNRALPEMLQ CSDMTVLERQ RARLKWQQEQ LQQQQLQQQE QQQSYFSELS GVFSSQPSHV  60
EGFQGGLMSG DSVLGDMVMT RQLKPDPGLE TAWPELVKVD MPGMGFGPCG YGNGPSFDMN  120
YAISRTSSCP PAVAAAVAGE VVEVKGKESV VSEKIGSAVG RESFKKRKVD KLQNLKVVAE  180
DDSKRIKACA EEGESKITGP NTNKSSSNNN NNKKESSTDT SKENSKVSEV QKPDYIHVRA  240
RRGQATDSHS LAERVRREKI SERMKYLQDL VPGCNKITGK AGMLDEIINY VQSLQRQVEF  300
LSMKLAAVNP RLDFNVENLF AKEVFPSCTT NFPTVGMSSE MANPPYLQVS PVQHVVSCCG  360
LEMGMNTPDM APRRTISAPV SIPDASFLDS SCFPQIQPSA TWDVELQNLY NVAFDQGRST  420
SFPSQPFTGS IEASNLKMEM *

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor that binds DNA to G box 5'-CACGTG-3' and, to a lower extent, to E-box 5'-CANNTG-3' in vitro. Binds to chromatin DNA of the FT gene and promotes its expression, and thus triggers flowering in response to blue light. {ECO:0000269\|PubMed:18988809, ECO:0000269\|PubMed:24130508}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Accumulates strongly in response to blue light due to reduced preventing 26S proteasome-mediated degradation in an ADO1/ZTL and ADO2/LKP2 dependent manner, but levels decrease in the absence of blue light via 26S proteasome degradation (at protein level). {ECO:0000269\|PubMed:24101505}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	EU532258	9e-75	EU532258.1 Gossypium barbadense clone I8-1 ISSR marker genomic sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_007042169.2	0.0	PREDICTED: transcription factor bHLH63
Swissprot	Q8GY61	1e-63	BH063_ARATH; Transcription factor bHLH63
TrEMBL	A0A061DZ54	0.0	A0A061DZ54_THECC; Cryptochrome-interacting basic-helix-loop-helix 1, putative isoform 1
STRING	EOX98000	0.0	(Theobroma cacao)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM5947	25	43

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G34530.1	4e-62	cryptochrome-interacting basic-helix-loop-helix 1

Link Out ? help Back to Top
Phytozome	Thecc1EG006871t1
Entrez Gene	18607768

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Motamayor JC, et al. The genome sequence of the most widely cultivated cacao type and its use to identify candidate genes regulating pod color. Genome Biol., 2013. 14(6): p. r53 [PMID:23731509] Yang D,Zhao W,Meng Y,Li H,Liu B A CIB1-LIKE transcription factor GmCIL10 from soybean positively regulates plant flowering. Sci China Life Sci, 2015. 58(3): p. 261-9 [PMID:25651969] Katsura Y, et al. An optogenetic system for interrogating the temporal dynamics of Akt. Sci Rep, 2015. 5: p. 14589 [PMID:26423353] Taslimi A, et al. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Nat. Chem. Biol., 2016. 12(6): p. 425-30 [PMID:27065233] Liu Q, et al. The Blue Light-Dependent Polyubiquitination and Degradation of Arabidopsis Cryptochrome2 Requires Multiple E3 Ubiquitin Ligases. Plant Cell Physiol., 2016. 57(10): p. 2175-2186 [PMID:27516416] Pathak GP, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res., 2017. 45(20): p. e167 [PMID:28431041] Duan L, et al. Understanding CRY2 interactions for optical control of intracellular signaling. Nat Commun, 2017. 8(1): p. 547 [PMID:28916751] Quejada JR, et al. Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1. Nucleic Acids Res., 2017. 45(20): p. e172 [PMID:29040770] Liu Y, et al. CIB1 and CO interact to mediate CRY2-dependent regulation of flowering. EMBO Rep., 2019. [PMID:30126927]