PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Sp_048190_wgha.t3
Common NameSOVF_048190
Organism
Spinacia oleracea
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia
Family ARF
Protein Properties Length: 809aa    MW: 90398.5 Da    PI: 6.4101
Description ARF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Sp_048190_wgha.t3genomeTBVRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1B3741.7e-23128229199
                        EEEE-..-HHHHTT-EE--HHH.HTT.......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-EEEEEE- CS
                 B3   1 ffkvltpsdvlksgrlvlpkkfaeeh.......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfvvFkld 85 
                        f+k+lt sd++++g +++p++ ae+        +++++  ++l+ +d++  +W++++i+r++++r++lt+GW+ Fv+a++L +gD+v+F   
  Sp_048190_wgha.t3 128 FCKTLTASDTSTHGGFSVPRRAAEKVfppldysQQPPA--QELVARDLHDVEWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFI-- 215
                        99*****************************8544444..48************************************************.. PP

                        SSSEE..EEEEE-S CS
                 B3  86 grsefelvvkvfrk 99 
                        ++++ +l+++++r+
  Sp_048190_wgha.t3 216 WNEKNQLLLGIRRA 229
                        8899999****997 PP
2Auxin_resp1187.8e-39254337183
         Auxin_resp   1 aahaastksvFevvYnPrastseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 
                        aahaa+t+s F+v++nPras+seF+++++k++ka++ ++vsvGmRf+m fete+ss rr++Gt++g+sdld+vrWpnS+Wrs+k
  Sp_048190_wgha.t3 254 AAHAANTNSCFTVFFNPRASPSEFIIPLSKYVKAVYhTRVSVGMRFRMLFETEESSVRRYMGTITGISDLDSVRWPNSHWRSVK 337
                        79*********************************9789******************************************985 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF1019361.18E-48115257IPR015300DNA-binding pseudobarrel domain
Gene3DG3DSA:2.40.330.107.0E-42121243IPR015300DNA-binding pseudobarrel domain
CDDcd100172.21E-21127228No hitNo description
PfamPF023621.3E-21128229IPR003340B3 DNA binding domain
PROSITE profilePS5086312.379128230IPR003340B3 DNA binding domain
SMARTSM010196.9E-24128230IPR003340B3 DNA binding domain
PfamPF065075.6E-34254337IPR010525Auxin response factor
PfamPF023097.8E-8700798IPR033389AUX/IAA domain
SuperFamilySSF542772.16E-7706785No hitNo description
PROSITE profilePS5174523.464712796IPR000270PB1 domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009734Biological Processauxin-activated signaling pathway
GO:0009908Biological Processflower development
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0005515Molecular Functionprotein binding
Sequence ? help Back to Top
Protein Sequence    Length: 809 aa     Download sequence    Send to blast
MKLSTSGLGQ QGNEGGEKKC LNSELWHACA GPLVCLPTAG SRVVYFPQGH SEQVAATTNR  60
DVDGQIPNYP SLPAQLICQL HNVTMHADVE TDEVYAQMTL QPLSPQEQKD IYLPADFGIP  120
SRQPTNYFCK TLTASDTSTH GGFSVPRRAA EKVFPPLDYS QQPPAQELVA RDLHDVEWKF  180
RHIFRGQPKR HLLTTGWSVF VSAKRLVAGD SVLFIWNEKN QLLLGIRRAT RPQTVMPSSV  240
LSSDSMHIGL LAAAAHAANT NSCFTVFFNP RASPSEFIIP LSKYVKAVYH TRVSVGMRFR  300
MLFETEESSV RRYMGTITGI SDLDSVRWPN SHWRSVKVGW DESTAGERQP RVSLWEIEPL  360
TTFPMYPSLF PLRLKRPWHA DNIDDAANSL MWLRGQGGEQ GLNSMNFQSL GMFPWMQTRA  420
DNLLPLGNQN QQYQALLSSN FQNLRGSDVL NPQMMQFQHP VQYLQQSTGQ IPMLQQQQQQ  480
QQQLFHPSIA QQLLQGQSQV SKDNLPLPQR ILPQEYFKQN EGLANQQLCG SEAYSLSNNE  540
MQSRQQHHTM SSSLGKADTS TANAELPSQS PVQNMMSSTR TEGISSLSDM SNVNHTMLNE  600
QTSQHPMVSL NSYTPINAFG DAVSLPPYPG KDASLETGNC SLDVQNHNVF GANLDSSGLV  660
LPMTMPTTYR NSSHDTDLCS IPSGFHNPMY GCNSNSPELH SEGQGDLQTP TRTFVKVYKS  720
GSVGRSLDIS RFSSYHELRE ELGQMFGIEG KLDDPLRSGW QLVFVDREND VLLLGDDPWE  780
AFVYNVSYIK ILSPEDVQKL GKHGTETYS
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4ldu_A1e-1641535845388Auxin response factor 5
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtAuxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Regulates both stamen and gynoecium maturation. Promotes jasmonic acid production. Partially redundant with ARF6. Involved in fruit initiation. Acts as an inhibitor to stop further carpel development in the absence of fertilization and the generation of signals required to initiate fruit and seed development. {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:16107481, ECO:0000269|PubMed:16829592}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00033PBMTransfer from AT5G37020Download
Motif logo
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by miR167. {ECO:0000269|PubMed:17021043}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_021854186.10.0auxin response factor 8 isoform X3
SwissprotQ9FGV10.0ARFH_ARATH; Auxin response factor 8
TrEMBLA0A0K9RMW90.0A0A0K9RMW9_SPIOL; Auxin response factor
STRINGXP_010675199.10.0(Beta vulgaris)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G37020.10.0auxin response factor 8
Publications ? help Back to Top
  1. Liu N, et al.
    Down-regulation of AUXIN RESPONSE FACTORS 6 and 8 by microRNA 167 leads to floral development defects and female sterility in tomato.
    J. Exp. Bot., 2014. 65(9): p. 2507-20
    [PMID:24723401]
  2. Minato N, et al.
    The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways.
    Sci Rep, 2014. 4: p. 7399
    [PMID:25492247]
  3. Ortega-Amaro MA, et al.
    Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance.
    Front Plant Sci, 2014. 5: p. 782
    [PMID:25653657]
  4. Wang Y, et al.
    MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.
    Plant Physiol., 2015. 168(3): p. 984-99
    [PMID:25941314]
  5. Barik S, et al.
    Coevolution Pattern and Functional Conservation or Divergence of miR167s and their targets across Diverse Plant Species.
    Sci Rep, 2015. 5: p. 14611
    [PMID:26459056]
  6. Pashkovskiy PP,Kartashov AV,Zlobin IE,Pogosyan SI,Kuznetsov VV
    Blue light alters miR167 expression and microRNA-targeted auxin response factor genes in Arabidopsis thaliana plants.
    Plant Physiol. Biochem., 2016. 104: p. 146-54
    [PMID:27031426]
  7. Mlotshwa S,Pruss GJ,MacArthur JL,Reed JW,Vance V
    Developmental Defects Mediated by the P1/HC-Pro Potyviral Silencing Suppressor Are Not Due to Misregulation of AUXIN RESPONSE FACTOR 8.
    Plant Physiol., 2016. 172(3): p. 1853-1861
    [PMID:27688620]
  8. Wójcikowska B,Gaj MD
    Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis.
    Plant Cell Rep., 2017. 36(6): p. 843-858
    [PMID:28255787]
  9. Zhang GZ, et al.
    Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis.
    Plant Cell Rep., 2017. 36(12): p. 1995-2006
    [PMID:29027578]
  10. Zheng K, et al.
    Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1813
    [PMID:29114256]
  11. Liu Z, et al.
    ARF2-ARF4 and ARF5 are Essential for Female and Male Gametophyte Development in Arabidopsis.
    Plant Cell Physiol., 2018. 59(1): p. 179-189
    [PMID:29145642]
  12. Ghelli R, et al.
    A Newly Identified Flower-Specific Splice Variant of AUXIN RESPONSE FACTOR8 Regulates Stamen Elongation and Endothecium Lignification in Arabidopsis.
    Plant Cell, 2018. 30(3): p. 620-637
    [PMID:29514943]