PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

SMil_00029962-RA_Salv

Organism

Salvia miltiorrhiza

Taxonomic ID

226208

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Mentheae; Salvia

Family

WRKY

Protein Properties

Length: 347aa MW: 37336.5 Da PI: 8.3296

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
SMil_00029962-RA_Salv	genome	NDCTCM	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	94.5	7.7e-30	99	157	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 +Dg++WrKYGqK kg+++pr+YYrCt + +Cpv+k+v+r+ +d +++++tYeg+Hnh+ SMil_00029962-RA_Salv 99 HDGCQWRKYGQKIAKGNPCPRAYYRCTVSsSCPVRKQVQRCIDDMSILITTYEGTHNHP 157 7************************999**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.1E-31	83	159	IPR003657	WRKY domain
SuperFamily	SSF118290	3.27E-27	91	159	IPR003657	WRKY domain
PROSITE profile	PS50811	28.288	93	159	IPR003657	WRKY domain
SMART	SM00774	5.2E-35	98	158	IPR003657	WRKY domain
Pfam	PF03106	1.9E-26	100	157	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 347 aa Download sequence Send to blast
LGSLAASDDD DESELVCLSL GRSSRKKINL ENDKLELGLN SRNLRSENSL DESKEEGSSD 60 VRSSPKHSRS NGEDEILQHN PLKKPRVSVR ALCNTQTMHD GCQWRKYGQK IAKGNPCPRA 120 YYRCTVSSSC PVRKQVQRCI DDMSILITTY EGTHNHPLPA SAAPMASATS SAATMLSCGS 180 TPFGYNFPRA TISSSQSHPT VILDLTAPKS TTLFSTTRSS PTSFNFSSNF PAINNATAPP 240 YSQPPLFSGT QNDQKIAAAI AHDPNFQSAL AAAFTSIVGK NNNSGGMNFH HLNFAQASTS 300 SAGCAASFMN RFGQPVNSQQ ANFAPLQMGE SKSRAMSVAD EGEQMKL

Sequence ? help Back to Top

Protein Sequence Length: 347 aa Download sequence Send to blast

LGSLAASDDD DESELVCLSL GRSSRKKINL ENDKLELGLN SRNLRSENSL DESKEEGSSD  60
VRSSPKHSRS NGEDEILQHN PLKKPRVSVR ALCNTQTMHD GCQWRKYGQK IAKGNPCPRA  120
YYRCTVSSSC PVRKQVQRCI DDMSILITTY EGTHNHPLPA SAAPMASATS SAATMLSCGS  180
TPFGYNFPRA TISSSQSHPT VILDLTAPKS TTLFSTTRSS PTSFNFSSNF PAINNATAPP  240
YSQPPLFSGT QNDQKIAAAI AHDPNFQSAL AAAFTSIVGK NNNSGGMNFH HLNFAQASTS  300
SAGCAASFMN RFGQPVNSQQ ANFAPLQMGE SKSRAMSVAD EGEQMKL

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	7e-20	90	159	2	74	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator involved in the transcriptional regulation of terpene biosynthesis in glandular trichomes (PubMed:24884371). Binds to the promoter of the linalool synthase TPS5 and promotes TPS5 gene transactivation (PubMed:24884371). In association with WRKY72B, contributes to basal defense against root-knot nematodes (RKNs) and potato aphids, as well as Mi-1-mediated gene-for-gene resistance to these pests (PubMed:20409007). Both WRKY72A and WRKY72B are not required for gene-for-gene resistance mediated by Pto, another tomato R gene (PubMed:20409007). {ECO:0000269\|PubMed:20409007, ECO:0000269\|PubMed:24884371}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by infection with the root-knot nematode (RKN) Meloidogyne incognita and potato aphids. {ECO:0000269\|PubMed:20409007}.

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KM823145	0.0	KM823145.1 Salvia miltiorrhiza WRKY protein (WRKY22) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_020552049.1	1e-93	probable WRKY transcription factor 72
Swissprot	A0A3Q7GFB5	5e-67	WK72A_SOLLC; WRKY transcription factor 72A
TrEMBL	A0A0D5YAD7	0.0	A0A0D5YAD7_SALMI; WRKY protein
STRING	Migut.L01332.1.p	1e-103	(Erythranthe guttata)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Asterids	OGEA1945	24	64

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G15130.1	8e-45	WRKY DNA-binding protein 72

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Spyropoulou EA,Haring MA,Schuurink RC RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters. BMC Genomics, 2014. 15: p. 402 [PMID:24884371]