PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

SMil_00021978-RA_Salv

Organism

Salvia miltiorrhiza

Taxonomic ID

226208

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Mentheae; Salvia

Family

WRKY

Protein Properties

Length: 590aa MW: 63075.1 Da PI: 6.5903

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
SMil_00021978-RA_Salv	genome	NDCTCM	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	97.6	7.9e-31	301	359	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 Dg++WrKYGqK kg+++pr+YYrCt+a gCpv+k+v+r+a+d++++++tYeg+Hnh+ SMil_00021978-RA_Salv 301 TDGCQWRKYGQKLAKGNPCPRAYYRCTMAaGCPVRKQVQRCADDTSILITTYEGNHNHP 359 7*************************99**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.4E-33	285	361	IPR003657	WRKY domain
SuperFamily	SSF118290	6.15E-28	293	361	IPR003657	WRKY domain
PROSITE profile	PS50811	30.171	295	361	IPR003657	WRKY domain
SMART	SM00774	1.5E-37	300	360	IPR003657	WRKY domain
Pfam	PF03106	2.9E-27	302	359	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0010200	Biological Process	response to chitin
GO:0016036	Biological Process	cellular response to phosphate starvation
GO:0045892	Biological Process	negative regulation of transcription, DNA-templated
GO:0080169	Biological Process	cellular response to boron-containing substance deprivation
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 590 aa Download sequence Send to blast
MATRGGLSFD PDHHSITRLI HHQAKTHFHN NQLKLQQILP RFPAPPPPSP PLMDAPVKSP 60 PTTTIQFPVS HGDHGKRKVL DEMDFFADKN EGGGGGGRDD DEEENVGIEH HRLDFKVNTG 120 LHLLTTANTG SEESGVDDGM SSIPENKRSK IEMAFLQSEM ERMNSENARL KEMLDQMTNN 180 YNGLQMHVLT MMHQRSDNQA GGATAEESKN GHDNGLLVPR QFMDLGFGGN EEGSLSPPEG 240 RIGRDMPPAP DKDVVGGDEP CSESKVQKLA HSPRSVDQAT EATMRKARVS VRARSEAAMI 300 TDGCQWRKYG QKLAKGNPCP RAYYRCTMAA GCPVRKQVQR CADDTSILIT TYEGNHNHPL 360 PPAAMAMAST TSSAARMLLS GSMPSADGIM NSNFLARTLL PCSSSMATIS ASAPFPTITL 420 DLTQSPNPLQ FPKPSNQFPL SFLNPSQNMA AAGSSAAAAL LPQIFGQALY NQSKFSGLQM 480 SSAQELDQGA HEHLSSPLPP SLHQACQQNQ LSETVNALAT DPNFTAALAA AISSLIGGGG 540 GPSPNNGGPN PNNNNGNNNN NNNNGNGNVT SSNNSSNGNN KLNSSGFQVN

Sequence ? help Back to Top

Protein Sequence Length: 590 aa Download sequence Send to blast

MATRGGLSFD PDHHSITRLI HHQAKTHFHN NQLKLQQILP RFPAPPPPSP PLMDAPVKSP  60
PTTTIQFPVS HGDHGKRKVL DEMDFFADKN EGGGGGGRDD DEEENVGIEH HRLDFKVNTG  120
LHLLTTANTG SEESGVDDGM SSIPENKRSK IEMAFLQSEM ERMNSENARL KEMLDQMTNN  180
YNGLQMHVLT MMHQRSDNQA GGATAEESKN GHDNGLLVPR QFMDLGFGGN EEGSLSPPEG  240
RIGRDMPPAP DKDVVGGDEP CSESKVQKLA HSPRSVDQAT EATMRKARVS VRARSEAAMI  300
TDGCQWRKYG QKLAKGNPCP RAYYRCTMAA GCPVRKQVQR CADDTSILIT TYEGNHNHPL  360
PPAAMAMAST TSSAARMLLS GSMPSADGIM NSNFLARTLL PCSSSMATIS ASAPFPTITL  420
DLTQSPNPLQ FPKPSNQFPL SFLNPSQNMA AAGSSAAAAL LPQIFGQALY NQSKFSGLQM  480
SSAQELDQGA HEHLSSPLPP SLHQACQQNQ LSETVNALAT DPNFTAALAA AISSLIGGGG  540
GPSPNNGGPN PNNNNGNNNN NNNNGNGNVT SSNNSSNGNN KLNSSGFQVN

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	1e-22	287	363	1	76	WRKY transcription factor 1
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Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the control of processes related to senescence and pathogen defense (PubMed:12000796). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:12000796). Activates the transcription of the SIRK gene and represses its own expression and that of the WRKY42 genes (PubMed:12000796). Modulates phosphate homeostasis and Pi translocation by regulating PHO1 expression (PubMed:25733771). {ECO:0000269\|PubMed:12000796, ECO:0000269\|PubMed:25733771}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00209	DAP	Transfer from AT1G62300	Download

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid, ethylene, jasmonic acid, pathogens, wounding and strongly during leaf senescence. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11722756}.

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KM823152	0.0	KM823152.1 Salvia miltiorrhiza WRKY protein (WRKY29) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_011100410.1	0.0	WRKY transcription factor 6 isoform X2
Swissprot	Q9C519	1e-134	WRKY6_ARATH; WRKY transcription factor 6
TrEMBL	A0A0D5YA16	0.0	A0A0D5YA16_SALMI; WRKY protein
STRING	XP_009804478.1	0.0	(Nicotiana sylvestris)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Asterids	OGEA945	24	92

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G62300.1	1e-105	WRKY family protein

Publications ? help Back to Top
Zhou F, et al. High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression. Plant J., 2004. 39(6): p. 920-32 [PMID:15341634] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P An extracellular subtilase switch for immune priming in Arabidopsis. PLoS Pathog., 2013. 9(6): p. e1003445 [PMID:23818851] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Singh V,Roy S,Singh D,Nandi AK Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. J. Biosci., 2014. 39(1): p. 119-26 [PMID:24499796] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Chai J,Liu J,Zhou J,Xing D Mitogen-activated protein kinase 6 regulates NPR1 gene expression and activation during leaf senescence induced by salicylic acid. J. Exp. Bot., 2014. 65(22): p. 6513-28 [PMID:25210078] Vermeirssen V,De Clercq I,Van Parys T,Van Breusegem F,Van de Peer Y Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress. Plant Cell, 2014. 26(12): p. 4656-79 [PMID:25549671] Xiao D, et al. SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE Directly Interacts with the Cytoplasmic Domain of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE and Negatively Regulates Leaf Senescence in Arabidopsis. Plant Physiol., 2015. 169(2): p. 1275-91 [PMID:26304848] Bakshi M, et al. WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis. BMC Plant Biol., 2015. 15: p. 305 [PMID:26718529] Huang Y,Feng CZ,Ye Q,Wu WH,Chen YF Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development. PLoS Genet., 2016. 12(2): p. e1005833 [PMID:26829043] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P Correction: An Extracellular Subtilase Switch for Immune Priming in Arabidopsis. PLoS Pathog., 2016. 12(11): p. e1006003 [PMID:27806116] Li H, et al. BZR1 Positively Regulates Freezing Tolerance via CBF-Dependent and CBF-Independent Pathways in Arabidopsis. Mol Plant, 2017. 10(4): p. 545-559 [PMID:28089951] Stetter MG,Benz M,Ludewig U Increased root hair density by loss of WRKY6 in Arabidopsis thaliana. PeerJ, 2017. 5: p. e2891 [PMID:28149680] Zhou X, et al. StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato. J. Exp. Bot., 2017. 68(5): p. 1265-1281 [PMID:28338870] Bakshi M, et al. Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation. Front Microbiol, 2017. 8: p. 1262 [PMID:28747898] Zhang Y, et al. DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6. Plant Cell Rep., 2018. 37(7): p. 981-992 [PMID:29574486]