PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Solyc09g014990.2.1

Common Name

SlWRKY33B

Organism

Solanum lycopersicum

Taxonomic ID

4081

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum; Lycopersicon

Family

WRKY

Protein Properties

Length: 530aa MW: 58673.9 Da PI: 7.9331

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Solyc09g014990.2.1	genome	ITAG	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	104.7	5.1e-33	197	254	2	60
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 +DgynWrKYGqK+vkgse+prsYY+Ct+++Cp+kkkver+ d++++ei+Y+g+Hnh+k Solyc09g014990.2.1 197 EDGYNWRKYGQKQVKGSENPRSYYKCTFPNCPTKKKVERNL-DGHITEIVYKGSHNHPK 254 8**************************************.*************85 PP
2	WRKY	106.1	1.8e-33	361	419	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 ldDgy+WrKYGqK+vkg+++prsYY+Ct++gCpv+k+ver+++d ++v++tYeg+Hnh+ Solyc09g014990.2.1 361 LDDGYRWRKYGQKVVKGNPNPRSYYKCTFTGCPVRKHVERASHDLRAVITTYEGKHNHD 419 59********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	23.388	191	255	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	1.1E-27	191	255	IPR003657	WRKY domain
SuperFamily	SSF118290	1.03E-24	192	255	IPR003657	WRKY domain
SMART	SM00774	3.1E-35	196	254	IPR003657	WRKY domain
Pfam	PF03106	8.3E-25	197	253	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	2.5E-37	346	421	IPR003657	WRKY domain
SuperFamily	SSF118290	2.35E-29	353	421	IPR003657	WRKY domain
PROSITE profile	PS50811	38.067	356	421	IPR003657	WRKY domain
SMART	SM00774	4.3E-39	361	420	IPR003657	WRKY domain
Pfam	PF03106	8.2E-26	362	419	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009409	Biological Process	response to cold
GO:0009414	Biological Process	response to water deprivation
GO:0009651	Biological Process	response to salt stress
GO:0010120	Biological Process	camalexin biosynthetic process
GO:0010200	Biological Process	response to chitin
GO:0010508	Biological Process	positive regulation of autophagy
GO:0042742	Biological Process	defense response to bacterium
GO:0050832	Biological Process	defense response to fungus
GO:0070370	Biological Process	cellular heat acclimation
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 530 aa Download sequence Send to blast
MASSGGNMNT FMNSFNSFSS SQFMTSSFSD LLSDNNNNNN SNNDNKNWGF SEDRSKSFPM 60 MNSSTSPASP SSYLAFPHSL SPSMLLDSPV LFNNSNTLSS PTTGSFGNLN SKEGNSEFSF 120 QSRPATSSSI FQSSAPRNSL EDLMTRQQQT TEFSTAKTGV KSEVAPIQSF SQENMSNNPA 180 PVHYCQPSQY VREQKAEDGY NWRKYGQKQV KGSENPRSYY KCTFPNCPTK KKVERNLDGH 240 ITEIVYKGSH NHPKPQSTRR SSSQSIQNLA YSNLDVTNQP NAFLENGQRD SFAVTDNSSA 300 SFGDDDVDQG SPISKSGEND ENEPEAKRWK GDNENEVISS ASRTVREPRI VVQTTSDIDI 360 LDDGYRWRKY GQKVVKGNPN PRSYYKCTFT GCPVRKHVER ASHDLRAVIT TYEGKHNHDV 420 PAARGSGSYA MNKPPSGSNN NNSMPVVPRP TVLANHSNQG MNFNDTFFNT TQIQPPITLQ 480 MLQSSGTSSY SGFGNSSGSY MNQMQHTNNS KPISKEEPKD DLFFSSFLN*

Sequence ? help Back to Top

Protein Sequence Length: 530 aa Download sequence Send to blast

MASSGGNMNT FMNSFNSFSS SQFMTSSFSD LLSDNNNNNN SNNDNKNWGF SEDRSKSFPM  60
MNSSTSPASP SSYLAFPHSL SPSMLLDSPV LFNNSNTLSS PTTGSFGNLN SKEGNSEFSF  120
QSRPATSSSI FQSSAPRNSL EDLMTRQQQT TEFSTAKTGV KSEVAPIQSF SQENMSNNPA  180
PVHYCQPSQY VREQKAEDGY NWRKYGQKQV KGSENPRSYY KCTFPNCPTK KKVERNLDGH  240
ITEIVYKGSH NHPKPQSTRR SSSQSIQNLA YSNLDVTNQP NAFLENGQRD SFAVTDNSSA  300
SFGDDDVDQG SPISKSGEND ENEPEAKRWK GDNENEVISS ASRTVREPRI VVQTTSDIDI  360
LDDGYRWRKY GQKVVKGNPN PRSYYKCTFT GCPVRKHVER ASHDLRAVIT TYEGKHNHDV  420
PAARGSGSYA MNKPPSGSNN NNSMPVVPRP TVLANHSNQG MNFNDTFFNT TQIQPPITLQ  480
MLQSSGTSSY SGFGNSSGSY MNQMQHTNNS KPISKEEPKD DLFFSSFLN*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	1e-33	197	422	8	78	Probable WRKY transcription factor 4
2lex_A	1e-33	197	422	8	78	Probable WRKY transcription factor 4
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Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Les.9238	0.0	callus\| flower\| fruit\| leaf\| root

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Highly expressed in roots, leaves and flowers, and at lower levels in stems, siliques and seeds. {ECO:0000269\|PubMed:18839316}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-TTGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Involved in defense responses. Required for resistance to the necrotrophic fungal pathogen B.cinerea (PubMed:17059405, PubMed:21990940). Regulates the antagonistic relationship between defense pathways mediating responses to the bacterial pathogen P. syringae and the necrotrophic pathogen B.cinerea (PubMed:17059405). Required for the phytoalexin camalexin synthesis following infection with B.cinerea. Acts as positive regulator of the camalexin biosynthetic genes PAD3 (CYP71B15) and CYP71A13 by binding to their promoters (PubMed:21498677, PubMed:22392279). Acts downstream of MPK3 and MPK6 in reprogramming the expression of camalexin biosynthetic genes, which drives the metabolic flow to camalexin production (PubMed:21498677). Functions with WRKY25 as positive regulator of salt stress response and abscisic acid (ABA) signaling (PubMed:18839316). Functions with WRKY25 and WRKY26 as positive regulator of plant thermotolerance by partially participating in ethylene-response signal transduction pathway (PubMed:21336597). The DNA-binding activity of WRKY33 is increased by SIB1 and SIB2 (PubMed:21990940). {ECO:0000269\|PubMed:18839316, ECO:0000269\|PubMed:21336597, ECO:0000269\|PubMed:21498677, ECO:0000269\|PubMed:21990940, ECO:0000269\|PubMed:22392279}.

Function -- GeneRIF ? help Back to Top
Molecular complementation and gene silencing confirmed that the two tomato WRKY genes (SlWRKY33A and SlWRKY33B) play a critical role similar to that of AtWRKY33 in plant stress responses.[WRKY33B] [PMID: 25969555]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00299	DAP	Transfer from AT2G38470	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Solyc09g014990.2.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salt stress (PubMed:18839316). Induced by infection with the necrotrophic fungal pathogen B.cinerea (PubMed:17059405, PubMed:21498677, PubMed:21990940). Induced by infection with the bacterial pathogen P.syringae pv. tomato DC3000 (PubMed:17059405). {ECO:0000269\|PubMed:17059405, ECO:0000269\|PubMed:18839316, ECO:0000269\|PubMed:21498677, ECO:0000269\|PubMed:21990940}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AC244886	5e-14	Solanum lycopersicum strain Heinz 1706 chromosome 10 clone sle-19g7 map 10, complete sequence

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_004246308.1	0.0	probable WRKY transcription factor 26
Swissprot	Q8S8P5	1e-125	WRK33_ARATH; Probable WRKY transcription factor 33
TrEMBL	Q06B53	0.0	Q06B53_SOLPE; WRKY-like transcription factor
STRING	Solyc09g014990.2.1	0.0	(Solanum lycopersicum)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Asterids	OGEA2286	24	60
Representative plant	OGRP14	17	875

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G38470.1	1e-117	WRKY DNA-binding protein 33

Link Out ? help Back to Top
Phytozome	Solyc09g014990.2.1
Entrez Gene	101268787

Publications ? help Back to Top
Wang Y,van der Hoeven RS,Nielsen R,Mueller LA,Tanksley SD Characteristics of the tomato nuclear genome as determined by sequencing undermethylated EcoRI digested fragments. Theor. Appl. Genet., 2005. 112(1): p. 72-84 [PMID:16208505] Brand LH,Kirchler T,Hummel S,Chaban C,Wanke D DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro. Plant Methods, 2010. 6: p. 25 [PMID:21108821] Brand LH, et al. Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA. PLoS ONE, 2013. 8(10): p. e75177 [PMID:24146751] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Zhou J, et al. Characterization of the promoter and extended C-terminal domain of Arabidopsis WRKY33 and functional analysis of tomato WRKY33 homologues in plant stress responses. J. Exp. Bot., 2015. 66(15): p. 4567-83 [PMID:25969555] Divi UK,Rahman T,Krishna P Gene expression and functional analyses in brassinosteroid-mediated stress tolerance. Plant Biotechnol. J., 2016. 14(1): p. 419-32 [PMID:25973891] Peskan-Berghöfer T, et al. Sustained exposure to abscisic acid enhances the colonization potential of the mutualist fungus Piriformospora indica on Arabidopsis thaliana roots. New Phytol., 2015. 208(3): p. 873-86 [PMID:26075497] Wang C, et al. The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum. Plant Physiol., 2015. 169(1): p. 856-72 [PMID:26143252] Wang C, et al. Arabidopsis Elongator subunit 2 positively contributes to resistance to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola. Plant J., 2015. 83(6): p. 1019-33 [PMID:26216741] Datta R, et al. Glutathione Regulates 1-Aminocyclopropane-1-Carboxylate Synthase Transcription via WRKY33 and 1-Aminocyclopropane-1-Carboxylate Oxidase by Modulating Messenger RNA Stability to Induce Ethylene Synthesis during Stress. Plant Physiol., 2015. 169(4): p. 2963-81 [PMID:26463088] Daumann M,Fischer M,Niopek-Witz S,Girke C,Möhlmann T Apoplastic Nucleoside Accumulation in Arabidopsis Leads to Reduced Photosynthetic Performance and Increased Susceptibility Against Botrytis cinerea. Front Plant Sci, 2015. 6: p. 1158 [PMID:26779190] Liu S,Bartnikas LM,Volko SM,Ausubel FM,Tang D Mutation of the Glucosinolate Biosynthesis Enzyme Cytochrome P450 83A1 Monooxygenase Increases Camalexin Accumulation and Powdery Mildew Resistance. Front Plant Sci, 2016. 7: p. 227 [PMID:26973671] Jiang Y,Yu D The WRKY57 Transcription Factor Affects the Expression of Jasmonate ZIM-Domain Genes Transcriptionally to Compromise Botrytis cinerea Resistance. Plant Physiol., 2016. 171(4): p. 2771-82 [PMID:27268959] Liao CJ,Lai Z,Lee S,Yun DJ,Mengiste T Arabidopsis HOOKLESS1 Regulates Responses to Pathogens and Abscisic Acid through Interaction with MED18 and Acetylation of WRKY33 and ABI5 Chromatin. Plant Cell, 2016. 28(7): p. 1662-81 [PMID:27317674] Birkenbihl RP,Kracher B,Roccaro M,Somssich IE Induced Genome-Wide Binding of Three Arabidopsis WRKY Transcription Factors during Early MAMP-Triggered Immunity. Plant Cell, 2017. 29(1): p. 20-38 [PMID:28011690] Nguyen CC, et al. Overexpression of oligouridylate binding protein 1b results in ABA hypersensitivity. Plant Signal Behav, 2017. 12(2): p. e1282591 [PMID:28112571] Liu S,Ziegler J,Zeier J,Birkenbihl RP,Somssich IE Botrytis cinerea B05.10 promotes disease development in Arabidopsis by suppressing WRKY33-mediated host immunity. Plant Cell Environ., 2017. 40(10): p. 2189-2206 [PMID:28708934] D'Ambrosio JM, et al. Phospholipase C2 Affects MAMP-Triggered Immunity by Modulating ROS Production. Plant Physiol., 2017. 175(2): p. 970-981 [PMID:28827453] Liu F, et al. Interactions of WRKY15 and WRKY33 transcription factors and their roles in the resistance of oilseed rape to Sclerotinia infection. Plant Biotechnol. J., 2018. 16(4): p. 911-925 [PMID:28929638] Crespo-Salvador Ó,Escamilla-Aguilar M,López-Cruz J,López-Rodas G,González-Bosch C Determination of histone epigenetic marks in Arabidopsis and tomato genes in the early response to Botrytis cinerea. Plant Cell Rep., 2018. 37(1): p. 153-166 [PMID:29119291]