PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Seita.8G089100.1.p

Organism

Setaria italica

Taxonomic ID

4555

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Panicodae; Paniceae; Cenchrinae; Setaria

Family

WRKY

Protein Properties

Length: 1359aa MW: 151493 Da PI: 6.5089

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Seita.8G089100.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	96.3	2e-30	1032	1090	2	59
--SS-EEEEEEE--TT.-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkg.sefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 dDgy+WrKYGqK vkg e+prsYYrCt+++Cp+kk+versa d++++ei+Y+g+Hnh+ Seita.8G089100.1.p 1032 DDGYSWRKYGQKLVKGiREHPRSYYRCTFPNCPTKKRVERSALDGQIIEIVYNGTHNHD 1090 8*************5689************************************8 PP
2	WRKY	97.1	1.2e-30	1186	1243	1	58
---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnh 58 ldDg++WrKYG+K+vk++++prsYY+C +agC+v+k+vers++dp++v++tY+g+Hnh Seita.8G089100.1.p 1186 LDDGFRWRKYGKKMVKENPYPRSYYKCLMAGCSVRKHVERSSRDPSTVITTYKGKHNH 1243 59******************************************************** PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
CDD	cd14798	1.26E-10	9	91	No hit	No description
SuperFamily	SSF52540	3.71E-52	140	410	IPR027417	P-loop containing nucleoside triphosphate hydrolase
Pfam	PF00931	3.5E-52	159	437	IPR002182	NB-ARC
Gene3D	G3DSA:3.40.50.300	1.1E-15	160	297	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PRINTS	PR00364	6.5E-12	173	188	No hit	No description
PRINTS	PR00364	6.5E-12	246	260	No hit	No description
PRINTS	PR00364	6.5E-12	343	357	No hit	No description
SuperFamily	SSF52058	4.42E-19	503	859	IPR032675	Leucine-rich repeat domain, L domain-like
Gene3D	G3DSA:3.80.10.10	2.1E-7	573	840	IPR032675	Leucine-rich repeat domain, L domain-like
PRINTS	PR00364	6.5E-12	643	659	No hit	No description
Gene3D	G3DSA:2.20.25.80	1.2E-27	1022	1093	IPR003657	WRKY domain
SuperFamily	SSF118290	1.09E-23	1026	1092	IPR003657	WRKY domain
PROSITE profile	PS50811	24.248	1026	1092	IPR003657	WRKY domain
SMART	SM00774	2.1E-34	1031	1091	IPR003657	WRKY domain
Pfam	PF03106	7.9E-24	1032	1090	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	2.9E-32	1172	1244	IPR003657	WRKY domain
SuperFamily	SSF118290	1.29E-26	1178	1244	IPR003657	WRKY domain
PROSITE profile	PS50811	31.456	1181	1243	IPR003657	WRKY domain
SMART	SM00774	9.9E-35	1186	1245	IPR003657	WRKY domain
Pfam	PF03106	7.7E-24	1187	1243	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043531	Molecular Function	ADP binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 1359 aa Download sequence Send to blast
MEVQMFSSSL GAMGSLLGKL RSLLVSPGDQ LPEPLKPQDK LELLKQDLEE IHTLLMDLSR 60 VEAPKTMAKL WMNQVRELSY DIEDCIDKMM HPPSNTGEEN RFDVEEFNTL MKQASDARKR 120 YHRYDLGRWA SNPTFRVVNR QVWVPTMDLV GIGDSRANLI NLLSNEAEKL MKVISVLGPV 180 GVGKTTLAKE VYRQMRGQFK CRAFVRASKT PDTRRLLRSI ISQIQRHQQP PHGLPVQELI 240 DYIRKHLQQK RYFIIIDGLW ETISWDIVNG AFPEGIDCSR ILITTDFEEV ALECSDYQSD 300 CIFKMEPLSR NNSRELFLNR LFGSKHECTE QLKDVSEKII EKCGGLPLAT ICIASILVSQ 360 SDNSELWLHV NECLSSFIRK NLTSEGMLSE IVGMSYNSLS DHLKTCLLYL SMYPEGYTFL 420 KTDLVKQWMA EGFISAVEGK DTGEVAEFYF DELVCRGLIL PNCIGFSDED IFYTVHSTVF 480 EVIRCKSMEE NFATVIDYSE AITKLSAKVR RLSLTFSSAK YATKPDGIAL SELRSLTFYG 540 LVNCLPSIME FKLLRVLILE FWGDKEELDI IGINKLFQLR YLRIRTDMTV KLPASMQELL 600 CLETLEMYAR VTTFPSDAFV LPRLLHLCLH DVINVPEGIG HMRSLRTLQC FDLSRISNGI 660 VWSLNEMTNL RDLRLTCTTA SREHHLKSNL IALISCLEKL GNLKTMILAP SASCTSINLC 720 CSGIVSSLPI SLGRLELLPP ICIFSRLPLC MGQLQKLRIL KIVLGELTGS DIYSIGRLQE 780 LTILSLYVRQ PTGELIVVNR AAFPVLKCFK LRCAIMRLAF QADAMPGLQS LKLEFNAHSG 840 EQNGNMLAGI EHLLNLQEVT GRIGAAPGAE ESDRVGAESV FKDAISKHSR LISFNLRIVH 900 SVDQEIPQFA DTPESCSGSD ETLNCSSISD GINEVHEVDT VQELLPFNNA SSTDNIVLLP 960 KYQMAPVGEK AATSTTLPQP IPPGYGCQEQ PSDGNTAAFS VPFQMTSSDV TTAIAPHHVV 1020 GSNTLSECRS SDDGYSWRKY GQKLVKGIRE HPRSYYRCTF PNCPTKKRVE RSALDGQIIE 1080 IVYNGTHNHD KPQNTSRGGS SMAAQPMQSG GSEAFEGKFG GMSGRNDEVG VSSSRAGNSE 1140 FDEHEEDFKR LRKDNEGEGI SMSGSRTTMV HEPRVALVTR SAIDILDDGF RWRKYGKKMV 1200 KENPYPRSYY KCLMAGCSVR KHVERSSRDP STVITTYKGK HNHGPLYPPP AMANQSSGAG 1260 QQQHAHGFGG QGWKGVSGSA SGQGTVGGSF VASTPDGVEL ELTPRALAKE EPKEDALSRC 1320 SPSILEHEEN QGPPLPSVHE TWSFQREIDV QDEVKPAV*

Sequence ? help Back to Top

Protein Sequence Length: 1359 aa Download sequence Send to blast

MEVQMFSSSL GAMGSLLGKL RSLLVSPGDQ LPEPLKPQDK LELLKQDLEE IHTLLMDLSR  60
VEAPKTMAKL WMNQVRELSY DIEDCIDKMM HPPSNTGEEN RFDVEEFNTL MKQASDARKR  120
YHRYDLGRWA SNPTFRVVNR QVWVPTMDLV GIGDSRANLI NLLSNEAEKL MKVISVLGPV  180
GVGKTTLAKE VYRQMRGQFK CRAFVRASKT PDTRRLLRSI ISQIQRHQQP PHGLPVQELI  240
DYIRKHLQQK RYFIIIDGLW ETISWDIVNG AFPEGIDCSR ILITTDFEEV ALECSDYQSD  300
CIFKMEPLSR NNSRELFLNR LFGSKHECTE QLKDVSEKII EKCGGLPLAT ICIASILVSQ  360
SDNSELWLHV NECLSSFIRK NLTSEGMLSE IVGMSYNSLS DHLKTCLLYL SMYPEGYTFL  420
KTDLVKQWMA EGFISAVEGK DTGEVAEFYF DELVCRGLIL PNCIGFSDED IFYTVHSTVF  480
EVIRCKSMEE NFATVIDYSE AITKLSAKVR RLSLTFSSAK YATKPDGIAL SELRSLTFYG  540
LVNCLPSIME FKLLRVLILE FWGDKEELDI IGINKLFQLR YLRIRTDMTV KLPASMQELL  600
CLETLEMYAR VTTFPSDAFV LPRLLHLCLH DVINVPEGIG HMRSLRTLQC FDLSRISNGI  660
VWSLNEMTNL RDLRLTCTTA SREHHLKSNL IALISCLEKL GNLKTMILAP SASCTSINLC  720
CSGIVSSLPI SLGRLELLPP ICIFSRLPLC MGQLQKLRIL KIVLGELTGS DIYSIGRLQE  780
LTILSLYVRQ PTGELIVVNR AAFPVLKCFK LRCAIMRLAF QADAMPGLQS LKLEFNAHSG  840
EQNGNMLAGI EHLLNLQEVT GRIGAAPGAE ESDRVGAESV FKDAISKHSR LISFNLRIVH  900
SVDQEIPQFA DTPESCSGSD ETLNCSSISD GINEVHEVDT VQELLPFNNA SSTDNIVLLP  960
KYQMAPVGEK AATSTTLPQP IPPGYGCQEQ PSDGNTAAFS VPFQMTSSDV TTAIAPHHVV  1020
GSNTLSECRS SDDGYSWRKY GQKLVKGIRE HPRSYYRCTF PNCPTKKRVE RSALDGQIIE  1080
IVYNGTHNHD KPQNTSRGGS SMAAQPMQSG GSEAFEGKFG GMSGRNDEVG VSSSRAGNSE  1140
FDEHEEDFKR LRKDNEGEGI SMSGSRTTMV HEPRVALVTR SAIDILDDGF RWRKYGKKMV  1200
KENPYPRSYY KCLMAGCSVR KHVERSSRDP STVITTYKGK HNHGPLYPPP AMANQSSGAG  1260
QQQHAHGFGG QGWKGVSGSA SGQGTVGGSF VASTPDGVEL ELTPRALAKE EPKEDALSRC  1320
SPSILEHEEN QGPPLPSVHE TWSFQREIDV QDEVKPAV*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
6j5t_C	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j5t_F	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j5t_G	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j5t_L	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j5t_O	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j5u_A	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j5v_A	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j5w_A	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j6i_C	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j6i_F	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j6i_G	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j6i_L	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
6j6i_O	8e-37	40	702	30	726	Disease resistance RPP13-like protein 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Disease resistance (R) protein that recognizes the AVR-Pia and AVR1-CO39 effector avirulence proteins from M.oryzae. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Contribution of RGA4 is required to recognize the effector avirulence proteins AVR-Pia and AVR1-CO39 from M.oryzae (PubMed:21251109, PubMed:23548743). Acts as a repressor of the RGA4-mediated cell death activation. Upon infection, recognition and binding of the AVR effectors relieve the RGA5-mediated repression and triggers the hypersensitive response (PubMed:25024433). Immune response triggered by the RGA4-RGA5 -mediated recognition of AVR1-CO39 confers resistance to X.oryzae pathovars (PubMed:27289079). {ECO:0000269\|PubMed:21251109, ECO:0000269\|PubMed:23548743, ECO:0000269\|PubMed:25024433, ECO:0000269\|PubMed:27289079}.
UniProt	Probable disease resistance protein. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. At the opposite of cultivars Aichi asahi and Sasanishiki, the cultivars Nipponbare, Mokoto and Hitomebore don't recognize the effector avirulence protein AVR-Pia from M.oryzae. {ECO:0000269\|PubMed:21251109}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Seita.8G089100.1.p

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_004979046.2	0.0	putative disease resistance RPP13-like protein 2 isoform X1
Swissprot	F7J0N2	0.0	RGA5R_ORYSJ; Disease resistance protein RGA5
Swissprot	Q2R8L1	0.0	RGA5S_ORYSJ; Disease resistance protein RGA5
TrEMBL	A0A368S5X3	0.0	A0A368S5X3_SETIT; Uncharacterized protein
STRING	Si028373m	0.0	(Setaria italica)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP5435	6	6

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G07100.2	3e-65	WRKY DNA-binding protein 26

Link Out ? help Back to Top
Phytozome	Seita.8G089100.1.p

Publications ? help Back to Top
Kikuchi S, et al. Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice. Science, 2003. 301(5631): p. 376-9 [PMID:12869764] Okuyama Y, et al. A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes. Plant J., 2011. 66(3): p. 467-79 [PMID:21251109] Cesari S, et al. The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding. Plant Cell, 2013. 25(4): p. 1463-81 [PMID:23548743] Césari S, et al. The NB-LRR proteins RGA4 and RGA5 interact functionally and physically to confer disease resistance. EMBO J., 2014. 33(17): p. 1941-59 [PMID:25024433] Cesari S,Bernoux M,Moncuquet P,Kroj T,Dodds PN A novel conserved mechanism for plant NLR protein pairs: the "integrated decoy" hypothesis. Front Plant Sci, 2014. 5: p. 606 [PMID:25506347] Huang D,Zhang Y,Zhao Y,Liu J,Peng YL Expression, purification, crystallization and preliminary X-ray diffraction analysis of the effector-interaction domain of the resistance protein RGA5-A from Oryza sativa L. japonica. Acta Crystallogr F Struct Biol Commun, 2015. 71(Pt 2): p. 171-4 [PMID:25664791] Hutin M, et al. Ectopic activation of the rice NLR heteropair RGA4/RGA5 confers resistance to bacterial blight and bacterial leaf streak diseases. Plant J., 2016. 88(1): p. 43-55 [PMID:27289079] Ortiz D, et al. Recognition of the Magnaporthe oryzae Effector AVR-Pia by the Decoy Domain of the Rice NLR Immune Receptor RGA5. Plant Cell, 2017. 29(1): p. 156-168 [PMID:28087830] Guo L, et al. Crystallization of the rice immune receptor RGA5A_S with the rice blast fungus effector AVR1-CO39 prepared via mixture and tandem strategies. Acta Crystallogr F Struct Biol Commun, 2018. 74(Pt 4): p. 262-267 [PMID:29633975] Guo L, et al. Specific recognition of two MAX effectors by integrated HMA domains in plant immune receptors involves distinct binding surfaces. Proc. Natl. Acad. Sci. U.S.A., 2018. 115(45): p. 11637-11642 [PMID:30355769]