PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

676721930

Organism

Sisymbrium irio

Taxonomic ID

3730

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Sisymbrieae; Sisymbrium

Family

bHLH

Protein Properties

Length: 604aa MW: 68910.6 Da PI: 5.0549

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
676721930	genome	VEGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	35.9	1.4e-11	336	387	5	55
HHHHHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHHHHH CS HLH 5 hnerErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksLq 55 + ++ErrRR+++N+++ Lr+l+P+ + Kl+ a+iL A++Y+k+Lq 676721930 336 NLMAERRRRKKLNERLYALRSLVPTiTKFFWAWKLDRASILGDAINYVKELQ 387 5589*******************774445789***************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	1.7E-18	28	168	IPR025610	Transcription factor MYC/MYB N-terminal
SuperFamily	SSF47459	4.58E-17	329	400	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	3.46E-13	330	389	No hit	No description
PROSITE profile	PS50888	15.993	331	386	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	3.3E-9	336	387	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	6.3E-13	337	392	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	3.2E-16	338	400	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009555	Biological Process	pollen development
GO:0048657	Biological Process	anther wall tapetum cell differentiation
GO:0005634	Cellular Component	nucleus
GO:0000978	Molecular Function	RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 604 aa Download sequence Send to blast
MHNLNGNEEK LSQQSHFERM ESSSMQNLSE KLRPLVGARA WDYCVLWRLN EDQRFVKWMG 60 CCCGGTQLIE ENGTEEFSFG GCRDVMFHHP RTKSCEFLAH LPSSIPLDSG IYAETLLTNQ 120 TGWLSESSEP GFMQETICTR VLIPVPGGLV ELFATRHVAE DQNVVDFVMG HCNMLMDETV 180 TINMMTADEV ESKPYGMLSG EVHQKGSKDE EIMNLTSPYD ISADQIRLNF LPQMSDYEAQ 240 QQLKMRSDYH HQALGYLPEN GNKEMMGMNP FNTVAEEGMP VMGEPSLLVN EQQVANDKET 300 NENGRLDTAS DCSDQIDDED DPKYKKKSGK QSQAKNLMAE RRRRKKLNER LYALRSLVPT 360 ITKFFWAWKL DRASILGDAI NYVKELQNEA KELQDELEEN SETEDGSNRQ QQGGMSLNGT 420 VVTGFHPGLS CNSNVPNLKQ DVDLENSNDK GQEMEGFQPQ VDVAQLDGRE FFVKVICEYK 480 PGGFTRLMEA LDSLGLEVTN ANTTRFLSLV SNVFKVEKND SEMVPAEHVR NSLLEITRNT 540 SRGWHDDQMA TGSMQNEKNE VDYQHYDDHH HHGGHHHLHD HQMNQSAHHH HHQHHQHINH 600 YQNQ

Sequence ? help Back to Top

Protein Sequence Length: 604 aa Download sequence Send to blast

MHNLNGNEEK LSQQSHFERM ESSSMQNLSE KLRPLVGARA WDYCVLWRLN EDQRFVKWMG  60
CCCGGTQLIE ENGTEEFSFG GCRDVMFHHP RTKSCEFLAH LPSSIPLDSG IYAETLLTNQ  120
TGWLSESSEP GFMQETICTR VLIPVPGGLV ELFATRHVAE DQNVVDFVMG HCNMLMDETV  180
TINMMTADEV ESKPYGMLSG EVHQKGSKDE EIMNLTSPYD ISADQIRLNF LPQMSDYEAQ  240
QQLKMRSDYH HQALGYLPEN GNKEMMGMNP FNTVAEEGMP VMGEPSLLVN EQQVANDKET  300
NENGRLDTAS DCSDQIDDED DPKYKKKSGK QSQAKNLMAE RRRRKKLNER LYALRSLVPT  360
ITKFFWAWKL DRASILGDAI NYVKELQNEA KELQDELEEN SETEDGSNRQ QQGGMSLNGT  420
VVTGFHPGLS CNSNVPNLKQ DVDLENSNDK GQEMEGFQPQ VDVAQLDGRE FFVKVICEYK  480
PGGFTRLMEA LDSLGLEVTN ANTTRFLSLV SNVFKVEKND SEMVPAEHVR NSLLEITRNT  540
SRGWHDDQMA TGSMQNEKNE VDYQHYDDHH HHGGHHHLHD HQMNQSAHHH HHQHHQHINH  600
YQNQ

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	324	345	KKKSGKQSQAKNLMAERRRRKK
2	339	346	ERRRRKKL

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther (PubMed:12535353). Binds E-box regions in the AHL16/TEK promoter. {ECO:0000269\|PubMed:12535353, ECO:0000269\|PubMed:24804694}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	676721930

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AJ511991	0.0	AJ511991.1 Brassica napus mRNA for bHLH transcription factor.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006409372.1	0.0	transcription factor ABORTED MICROSPORES
Swissprot	Q9ZVX2	0.0	AMS_ARATH; Transcription factor ABORTED MICROSPORES
TrEMBL	V4LJU2	0.0	V4LJU2_EUTSA; Uncharacterized protein
STRING	XP_006409372.1	0.0	(Eutrema salsugineum)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM7589	27	41

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G16910.1	0.0	bHLH family protein

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Jiang J,Zhang Z,Cao J Pollen wall development: the associated enzymes and metabolic pathways. Plant Biol (Stuttg), 2013. 15(2): p. 249-63 [PMID:23252839] Xu J, et al. ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis. Plant Cell, 2014. 26(4): p. 1544-1556 [PMID:24781116] Qian H, et al. Trace concentrations of imazethapyr (IM) affect floral organs development and reproduction in Arabidopsis thaliana: IM-induced inhibition of key genes regulating anther and pollen biosynthesis. Ecotoxicology, 2015. 24(1): p. 163-71 [PMID:25348600] Xiong SX, et al. The transcription factors MS188 and AMS form a complex to activate the expression of CYP703A2 for sporopollenin biosynthesis in Arabidopsis thaliana. Plant J., 2016. 88(6): p. 936-946 [PMID:27460657] Ferguson AC, et al. Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis. New Phytol., 2017. 213(2): p. 778-790 [PMID:27787905] Lou Y, et al. Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for anther development in Arabidopsis thaliana. New Phytol., 2018. 217(1): p. 378-391 [PMID:28940573] Bi H,Fan W,Zhang P C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis. Front Plant Sci, 2017. 8: p. 1689 [PMID:29021807]