PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Sobic.004G065900.1.p

Common Name

Sb04g005520, SORBIDRAFT_04g005520

Organism

Sorghum bicolor

Taxonomic ID

4558

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Sorghinae; Sorghum

Family

WRKY

Protein Properties

Length: 365aa MW: 38952.1 Da PI: 8.3762

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Sobic.004G065900.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	95.8	3e-30	193	252	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 ++Dgy+WrKYGqK+ k++++pr+Y+rC++a Cpvkkkv+rsa+d+++++ tYegeHnh Sobic.004G065900.1.p 193 VKDGYQWRKYGQKVTKDNPCPRAYFRCSFApACPVKKKVQRSADDTSILVATYEGEHNHG 252 58*************************99**************************5 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	26.314	188	254	IPR003657	WRKY domain
SuperFamily	SSF118290	5.1E-26	192	254	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	2.2E-29	192	254	IPR003657	WRKY domain
SMART	SM00774	4.4E-36	193	253	IPR003657	WRKY domain
Pfam	PF03106	4.3E-25	194	251	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0002237	Biological Process	response to molecule of bacterial origin
GO:0002238	Biological Process	response to molecule of fungal origin
GO:0009409	Biological Process	response to cold
GO:0009414	Biological Process	response to water deprivation
GO:0009611	Biological Process	response to wounding
GO:0009623	Biological Process	response to parasitic fungus
GO:0009651	Biological Process	response to salt stress
GO:0009685	Biological Process	gibberellin metabolic process
GO:0009723	Biological Process	response to ethylene
GO:0009737	Biological Process	response to abscisic acid
GO:0009751	Biological Process	response to salicylic acid
GO:0009753	Biological Process	response to jasmonic acid
GO:0009938	Biological Process	negative regulation of gibberellic acid mediated signaling pathway
GO:0010200	Biological Process	response to chitin
GO:0031347	Biological Process	regulation of defense response
GO:0045892	Biological Process	negative regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:1990841	Molecular Function	promoter-specific chromatin binding

Sequence ? help Back to Top
Protein Sequence Length: 365 aa Download sequence Send to blast
MDPWISSQPS LSLDLHVGLP PLSLHQAPVA AVALARPKVL VEENFLPPKK EPEVAALETE 60 LHRMSEENRR LTEALAAVAS KYEALRSQYT EMVAAAAAGT NNNPSSTSEG GSVSPSRKRK 120 SESMDTAPAP PAAAQQQQQH GTHLHQQQHP GLAAPDQNEC TSGEPCKRIR EECKPKVSKL 180 YVHADPADLS LVVKDGYQWR KYGQKVTKDN PCPRAYFRCS FAPACPVKKK VQRSADDTSI 240 LVATYEGEHN HGQPPPAAPS QAAHDGSAAP GATKNAAAVA KPPSPPRPAA PAPAPAPHRP 300 QLQLLQQEGV AMNVEQPVAA AASEMIRRNL AEQMAMTLTR DPSFKAALVT ALSGRILELS 360 PTKD*

Sequence ? help Back to Top

Protein Sequence Length: 365 aa Download sequence Send to blast

MDPWISSQPS LSLDLHVGLP PLSLHQAPVA AVALARPKVL VEENFLPPKK EPEVAALETE  60
LHRMSEENRR LTEALAAVAS KYEALRSQYT EMVAAAAAGT NNNPSSTSEG GSVSPSRKRK  120
SESMDTAPAP PAAAQQQQQH GTHLHQQQHP GLAAPDQNEC TSGEPCKRIR EECKPKVSKL  180
YVHADPADLS LVVKDGYQWR KYGQKVTKDN PCPRAYFRCS FAPACPVKKK VQRSADDTSI  240
LVATYEGEHN HGQPPPAAPS QAAHDGSAAP GATKNAAAVA KPPSPPRPAA PAPAPAPHRP  300
QLQLLQQEGV AMNVEQPVAA AASEMIRRNL AEQMAMTLTR DPSFKAALVT ALSGRILELS  360
PTKD*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	6e-22	178	255	1	75	WRKY transcription factor 1
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	115	120	SRKRKS

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Sbi.11937	0.0	embryo\| leaf\| root

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Highly expressed in aleurone cells (PubMed:15047897, PubMed:16623886). In seeds, predominantly present in the plumule, radicle and scutellum of the embryo (PubMed:16623886). Expressed in roots, stems, young leaves and spikelets (PubMed:16919842). {ECO:0000269\|PubMed:15047897, ECO:0000269\|PubMed:16623886, ECO:0000269\|PubMed:16919842}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription repressor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:18175928, PubMed:15047897, PubMed:16623886). Represses specifically gibberellic acid (GA)-induced promoters in aleurone cells, probably by interfering with GAM1 (PubMed:15047897, PubMed:16623886, PubMed:19199048). Regulates, probably indirectly, the activation of defense-related genes such as GF14E during defense response (PubMed:18175928, PubMed:26851365, PubMed:26467468). Modulates plant innate immunity against X.oryzae pv. oryzae (Xoo) (PubMed:21961049). Confers resistance to the virulent bacterial pathogen Xoo 13751, probably via the regulation of NPR1 and PR1b defense signaling pathways (PubMed:16919842). {ECO:0000269\|PubMed:15047897, ECO:0000269\|PubMed:16623886, ECO:0000269\|PubMed:16919842, ECO:0000269\|PubMed:18175928, ECO:0000269\|PubMed:19199048, ECO:0000269\|PubMed:21961049, ECO:0000269\|PubMed:26467468, ECO:0000269\|PubMed:26851365}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Sobic.004G065900.1.p

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by biotic elicitors (e.g. fungal chitin oligosaccharide and fungal cerebroside elicitors) and pathogen infection (e.g. the compatible pathogenic fungus M.grisea race 007, M. grisea crabgrass BR29) (PubMed:18175928, PubMed:19772648, PubMed:23462973). Accumulates in response to M.oryzae (PubMed:21726398). Triggered by defense signaling molecules, such as salicylic acid (SA), methyl jasmonate (MeJA), 1-aminocyclo-propane-1-carboxylic acid (ACC), wounding and pathogen infection (e.g. X.oryzae) (PubMed:16919842). Repressed by gibberellic acid (GA) (at protein level) (PubMed:15047897, PubMed:19199048). Induced by abscisic acid (ABA) in aleurone cells, roots and leaves (PubMed:15618416, PubMed:16623886). Accumulates in response to uniconazole, a GA biosynthesis inhibitor (PubMed:16623886). Triggered strongly by cold in leaves, stems and developing spikes, but moderately by drought and salt stresses (PubMed:14645724, PubMed:23495849). {ECO:0000269\|PubMed:14645724, ECO:0000269\|PubMed:15047897, ECO:0000269\|PubMed:15618416, ECO:0000269\|PubMed:16623886, ECO:0000269\|PubMed:16919842, ECO:0000269\|PubMed:18175928, ECO:0000269\|PubMed:19199048, ECO:0000269\|PubMed:19772648, ECO:0000269\|PubMed:21726398, ECO:0000269\|PubMed:23462973, ECO:0000269\|PubMed:23495849}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_002451666.1	0.0	WRKY transcription factor WRKY71
Swissprot	Q6QHD1	1e-155	WRK71_ORYSJ; WRKY transcription factor WRKY71
TrEMBL	C5XWV9	0.0	C5XWV9_SORBI; Uncharacterized protein
STRING	Sb04g005520.1	0.0	(Sorghum bicolor)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP1822	38	103
Representative plant	OGRP14	17	875

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G80840.1	3e-52	WRKY DNA-binding protein 40

Link Out ? help Back to Top
Phytozome	Sobic.004G065900.1.p
Entrez Gene	8077628

Publications ? help Back to Top
Rabbani MA, et al. Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses. Plant Physiol., 2003. 133(4): p. 1755-67 [PMID:14645724] Zhang Y,Wang L The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evol. Biol., 2005. 5: p. 1 [PMID:15629062] Wu KL,Guo ZJ,Wang HH,Li J The WRKY family of transcription factors in rice and Arabidopsis and their origins. DNA Res., 2005. 12(1): p. 9-26 [PMID:16106749] Xie Z, et al. Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells. Plant J., 2006. 46(2): p. 231-42 [PMID:16623886] Zou X,Neuman D,Shen QJ Interactions of two transcriptional repressors and two transcriptional activators in modulating gibberellin signaling in aleurone cells. Plant Physiol., 2008. 148(1): p. 176-86 [PMID:18621977] Zhang ZL, et al. A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells. Plant Mol. Biol., 2009. 70(1-2): p. 139-51 [PMID:19199048] Berri S, et al. Characterization of WRKY co-regulatory networks in rice and Arabidopsis. BMC Plant Biol., 2009. 9: p. 120 [PMID:19772648] Delteil A, et al. Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence. Mol. Plant Pathol., 2012. 13(1): p. 72-82 [PMID:21726398] Peng Y,Bartley LE,Canlas P,Ronald PC OsWRKY IIa Transcription Factors Modulate Rice Innate Immunity. Rice (N Y), 2010. 3(1): p. 36-42 [PMID:21961049] Chujo T, et al. OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate immune responses in rice against rice blast fungus. Plant Mol. Biol., 2013. 82(1-2): p. 23-37 [PMID:23462973] Kovalchuk N, et al. Optimization of TaDREB3 gene expression in transgenic barley using cold-inducible promoters. Plant Biotechnol. J., 2013. 11(6): p. 659-70 [PMID:23495849] Basu S,Roychoudhury A Expression profiling of abiotic stress-inducible genes in response to multiple stresses in rice (Oryza sativa L.) varieties with contrasting level of stress tolerance. Biomed Res Int, 2014. 2014: p. 706890 [PMID:25110688] Liu Q, et al. OsGF14b Positively Regulates Panicle Blast Resistance but Negatively Regulates Leaf Blast Resistance in Rice. Mol. Plant Microbe Interact., 2016. 29(1): p. 46-56 [PMID:26467468] Liu Q, et al. OsGF14e positively regulates panicle blast resistance in rice. Biochem. Biophys. Res. Commun., 2016. 471(1): p. 247-52 [PMID:26851365]