PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Rsa1.0_05893.1_g00002.1

Organism

Raphanus sativus

Taxonomic ID

3726

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus

Family

WRKY

Protein Properties

Length: 309aa MW: 34500.5 Da PI: 7.4973

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Rsa1.0_05893.1_g00002.1	genome	RGD	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	91.3	7.3e-29	151	211	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 dD ++WrKYGqK++ g++fprsYYrCt++ +C+++k+v+rs++dp+v+e tY+g+H+++ Rsa1.0_05893.1_g00002.1 151 DDVFSWRKYGQKDILGAKFPRSYYRCTHRstqNCWATKQVQRSDSDPTVFELTYRGRHTCQ 211 8*************************9999*************************97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	21.735	145	208	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	3.6E-27	146	212	IPR003657	WRKY domain
SuperFamily	SSF118290	1.2E-24	149	212	IPR003657	WRKY domain
SMART	SM00774	3.6E-38	150	212	IPR003657	WRKY domain
Pfam	PF03106	1.1E-25	151	211	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 309 aa Download sequence Send to blast
MEGQKAMLAW EQKKLQSEVS LGLEAARKLQ ARISEASPSS SSSYPAAETN EILMEQILSS 60 YEKVLVMLNR SSSPSVQPFP ATVAVVPVVN SGGVPESPAS INGSPRSEDG GGSSESHRQD 120 NIFNSRKRKM LPKWTEKVRI SPERGLEGPQ DDVFSWRKYG QKDILGAKFP RSYYRCTHRS 180 TQNCWATKQV QRSDSDPTVF ELTYRGRHTC QQGTAHPPPA SPEKRNTRPK LTTTTAQKPN 240 DLLESLKTSL TVRTEGLDDA DNVFSFPNTP PPFYDYGTIN DEFSSPIFDV VDWLNPTAFP 300 TFLPESIYY

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5w3x_B	3e-19	150	219	16	79	Disease resistance protein RRS1
5w3x_D	3e-19	150	219	16	79	Disease resistance protein RRS1
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	124	129	SRKRKM

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:20409006). May regulate the early events of leaf senescence (PubMed:17369373, PubMed:20409006). Negatively regulates the expression of ESR/ESP (PubMed:20409006). Together with WRKY46 and WRKY70, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2 (PubMed:22325892). {ECO:0000269\|PubMed:17369373, ECO:0000269\|PubMed:20409006, ECO:0000269\|PubMed:22325892}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Rsa1.0_05893.1_g00002.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid (SA) (PubMed:22268143, PubMed:22325892). Strong accumulation during leaf senescence (PubMed:22268143). Down-regulated by jasmonate. Triggered by P.syringae (PubMed:22325892). {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11506370, ECO:0000269\|PubMed:17369373, ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22325892}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KC246582	0.0	KC246582.1 Brassica napus WRKY transcription factor 53.2 (WRKY53.2) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_018462573.1	0.0	PREDICTED: probable WRKY transcription factor 53
Swissprot	Q9SUP6	1e-147	WRK53_ARATH; Probable WRKY transcription factor 53
TrEMBL	A0A3P6G554	1e-178	A0A3P6G554_BRAOL; Uncharacterized protein
STRING	Bra013732.1-P	1e-177	(Brassica rapa)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1933	27	83

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G23810.1	1e-131	WRKY family protein

Publications ? help Back to Top
Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P An extracellular subtilase switch for immune priming in Arabidopsis. PLoS Pathog., 2013. 9(6): p. e1003445 [PMID:23818851] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Singh V,Roy S,Singh D,Nandi AK Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. J. Biosci., 2014. 39(1): p. 119-26 [PMID:24499796] Zhang H, et al. Arabidopsis AtERF15 positively regulates immunity against Pseudomonas syringae pv. tomato DC3000 and Botrytis cinerea. Front Plant Sci, 2015. 6: p. 686 [PMID:26388886] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P Correction: An Extracellular Subtilase Switch for Immune Priming in Arabidopsis. PLoS Pathog., 2016. 12(11): p. e1006003 [PMID:27806116] Chen X, et al. POWERDRESS interacts with HISTONE DEACETYLASE 9 to promote aging in Arabidopsis. Elife, 2017. [PMID:27873573] Nie P, et al. Induced Systemic Resistance against Botrytis cinerea by Bacillus cereus AR156 through a JA/ET- and NPR1-Dependent Signaling Pathway and Activates PAMP-Triggered Immunity in Arabidopsis. Front Plant Sci, 2017. 8: p. 238 [PMID:28293243] Ren Y,Li Y,Jiang Y,Wu B,Miao Y Phosphorylation of WHIRLY1 by CIPK14 Shifts Its Localization and Dual Functions in Arabidopsis. Mol Plant, 2017. 10(5): p. 749-763 [PMID:28412544] Sarkar S, et al. Interaction of Arabidopsis TGA3 and WRKY53 transcription factors on Cestrum yellow leaf curling virus (CmYLCV) promoter mediates salicylic acid-dependent gene expression in planta. Planta, 2018. 247(1): p. 181-199 [PMID:28913593] Crespo-Salvador Ó,Escamilla-Aguilar M,López-Cruz J,López-Rodas G,González-Bosch C Determination of histone epigenetic marks in Arabidopsis and tomato genes in the early response to Botrytis cinerea. Plant Cell Rep., 2018. 37(1): p. 153-166 [PMID:29119291] Yuan N, et al. STRESS INDUCED FACTOR 2, a Leucine-Rich Repeat Kinase Regulates Basal Plant Pathogen Defense. Plant Physiol., 2018. 176(4): p. 3062-3080 [PMID:29463771]