PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Rsa1.0_00548.1_g00007.1
Organism
Raphanus sativus
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus
Family ARF
Protein Properties Length: 820aa    MW: 90556.5 Da    PI: 6.3993
Description ARF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Rsa1.0_00548.1_g00007.1genomeRGDView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1B372.84.2e-23127228199
                              EEEE-..-HHHHTT-EE--HHH.HTT......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-EE CS
                       B3   1 ffkvltpsdvlksgrlvlpkkfaeeh......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfv 80 
                              f+k+lt sd++++g +++p++ ae+       +++++ + +l  +d++  +W++++i+r++++r++lt+GW+ Fv+a++L +gD+v
  Rsa1.0_00548.1_g00007.1 127 FCKTLTASDTSTHGGFSVPRRAAEKVfppldySLQPPAQ-ELLARDLHDVEWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSV 211
                              99***********************9*****97777664.9********************************************* PP

                              EEEE-SSSEE..EEEEE-S CS
                       B3  81 vFkldgrsefelvvkvfrk 99 
                              +F   ++++ +l+++++r+
  Rsa1.0_00548.1_g00007.1 212 IFI--RNEKNQLFLGIRRA 228
                              ***..5688889***9997 PP
2Auxin_resp110.71.5e-36253336183
               Auxin_resp   1 aahaastksvFevvYnPrastseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 
                              aaha +t+s F+v+++Pras+seFv++++k+ ka+  +++svGmRf+m fete+ss rr++Gt++g+sdld+vrWpnS+Wrs+k
  Rsa1.0_00548.1_g00007.1 253 AAHASATNSCFTVFFHPRASQSEFVIQLSKYIKAVFhTRISVGMRFRMLFETEESSVRRYMGTITGISDLDSVRWPNSHWRSVK 336
                              799******************************986599******************************************985 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF1019361.31E-47113256IPR015300DNA-binding pseudobarrel domain
Gene3DG3DSA:2.40.330.107.2E-40120242IPR015300DNA-binding pseudobarrel domain
CDDcd100171.74E-20126222No hitNo description
PROSITE profilePS5086312.28127229IPR003340B3 DNA binding domain
PfamPF023625.5E-21127228IPR003340B3 DNA binding domain
SMARTSM010199.3E-23127229IPR003340B3 DNA binding domain
PfamPF065076.3E-32253336IPR010525Auxin response factor
SuperFamilySSF542772.49E-7708788No hitNo description
PROSITE profilePS5174523.082714798IPR000270PB1 domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009733Biological Processresponse to auxin
GO:0009908Biological Processflower development
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0005515Molecular Functionprotein binding
Sequence ? help Back to Top
Protein Sequence    Length: 820 aa     Download sequence    Send to blast
MKLSTSGLVQ QGSHEGEKCL NSELWHACAG PLVSLPSSGS RVVYFPQGHS EQVAATTNKE  60
VDGHIPNYPS LPPQLICQLH NVTMHADVET DEVYAQMTLQ PLTPEEQKET FVPIELGIPS  120
KQPSNYFCKT LTASDTSTHG GFSVPRRAAE KVFPPLDYSL QPPAQELLAR DLHDVEWKFR  180
HIFRGQPKRH LLTTGWSVFV SAKRLVAGDS VIFIRNEKNQ LFLGIRRATR PQTIVPSSVL  240
SSDSMHIGLL AAAAHASATN SCFTVFFHPR ASQSEFVIQL SKYIKAVFHT RISVGMRFRM  300
LFETEESSVR RYMGTITGIS DLDSVRWPNS HWRSVKVGWD ESTAGERQPR VSLWEIEPLT  360
TFPMYPSLFP LRLKRPWHAG TSSLHDGRGE LGSGLPWLRG GGGEQQGLLP LNYPSVGLFP  420
WMQQRLDLAQ LGTDNNQQYQ AMLAAGLQNM GGGDPLRQQF VQLQEPHHQY LQQSASHNSD  480
LMLQQQQQQQ QQASHHLMHA QTQIMSENLP LRQEVSKQPA GGQQSVDQNA YLNAFKMQNG  540
QLQQQWQQHS DMPSPSFTDA SNKYGTTASP MQQNSASPGS AGQHGSNLLN FSITGQSVLP  600
EQLMGEGWSP KASNTFSEPL SLPQAYAGGK SLALEPSSAN VNPSVFGVDP GSSGLFLPST  660
VPRFASSSGG DAEAYPISLA DSGFENSLYN SCMQDTTHEL LHGVGQINSS NQTKNFVKVY  720
KSGSVGRSLD ISRFSSYHEL REELGKMFAI EGLLEDPLRS GWQLVFVDKE NDILLLGDDP  780
WESFVSNVWY IKILSPEDVQ EMGDHGEGSG GSLPQNPTHL
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4ldu_A1e-154835739388Auxin response factor 5
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtAuxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Regulates both stamen and gynoecium maturation. Promotes jasmonic acid production. Partially redundant with ARF6. Involved in fruit initiation. Acts as an inhibitor to stop further carpel development in the absence of fertilization and the generation of signals required to initiate fruit and seed development. {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:16107481, ECO:0000269|PubMed:16829592}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00033PBMTransfer from AT5G37020Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapRsa1.0_00548.1_g00007.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by miR167. {ECO:0000269|PubMed:17021043}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF0421960.0AF042196.1 Arabidopsis thaliana auxin response factor 8 (ARF8) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_018483964.10.0PREDICTED: auxin response factor 8
SwissprotQ9FGV10.0ARFH_ARATH; Auxin response factor 8
TrEMBLA0A397ZJ870.0A0A397ZJ87_BRACM; Auxin response factor
STRINGBra028110.1-P0.0(Brassica rapa)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM13442894
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G37020.10.0auxin response factor 8
Publications ? help Back to Top
  1. Liu N, et al.
    Down-regulation of AUXIN RESPONSE FACTORS 6 and 8 by microRNA 167 leads to floral development defects and female sterility in tomato.
    J. Exp. Bot., 2014. 65(9): p. 2507-20
    [PMID:24723401]
  2. Minato N, et al.
    The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways.
    Sci Rep, 2014. 4: p. 7399
    [PMID:25492247]
  3. Ortega-Amaro MA, et al.
    Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance.
    Front Plant Sci, 2014. 5: p. 782
    [PMID:25653657]
  4. Wang Y, et al.
    MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.
    Plant Physiol., 2015. 168(3): p. 984-99
    [PMID:25941314]
  5. Barik S, et al.
    Coevolution Pattern and Functional Conservation or Divergence of miR167s and their targets across Diverse Plant Species.
    Sci Rep, 2015. 5: p. 14611
    [PMID:26459056]
  6. Pashkovskiy PP,Kartashov AV,Zlobin IE,Pogosyan SI,Kuznetsov VV
    Blue light alters miR167 expression and microRNA-targeted auxin response factor genes in Arabidopsis thaliana plants.
    Plant Physiol. Biochem., 2016. 104: p. 146-54
    [PMID:27031426]
  7. Mlotshwa S,Pruss GJ,MacArthur JL,Reed JW,Vance V
    Developmental Defects Mediated by the P1/HC-Pro Potyviral Silencing Suppressor Are Not Due to Misregulation of AUXIN RESPONSE FACTOR 8.
    Plant Physiol., 2016. 172(3): p. 1853-1861
    [PMID:27688620]
  8. Wójcikowska B,Gaj MD
    Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis.
    Plant Cell Rep., 2017. 36(6): p. 843-858
    [PMID:28255787]
  9. Zhang GZ, et al.
    Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis.
    Plant Cell Rep., 2017. 36(12): p. 1995-2006
    [PMID:29027578]
  10. Zheng K, et al.
    Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1813
    [PMID:29114256]
  11. Liu Z, et al.
    ARF2-ARF4 and ARF5 are Essential for Female and Male Gametophyte Development in Arabidopsis.
    Plant Cell Physiol., 2018. 59(1): p. 179-189
    [PMID:29145642]
  12. Ghelli R, et al.
    A Newly Identified Flower-Specific Splice Variant of AUXIN RESPONSE FACTOR8 Regulates Stamen Elongation and Endothecium Lignification in Arabidopsis.
    Plant Cell, 2018. 30(3): p. 620-637
    [PMID:29514943]