PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

RrC264_p4

Organism

Raphanus raphanistrum

Taxonomic ID

109996

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus

Family

NF-YC

Protein Properties

Length: 872aa MW: 96146.1 Da PI: 5.6178

Description

NF-YC family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
RrC264_p4	genome	MSU	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	NF-YC	175.6	4.9e-55	58	161	1	101
NF-YC 1 qlksfwekq...iekatdfknhelPlarikkilkadedvkmisaeaPvllskacelfileltlrswlhaeenkrrtlkksdiaaavtrtdifdflvdivp 97 ql++fwe+q iek+tdfknh+lPlarikki+kadedv+misaeaPv++++ace+fileltlrsw+h+eenkrrtl+k+diaaavtrtdifdflvdivp RrC264_p4 58 QLQVFWENQfkeIEKTTDFKNHSLPLARIKKIMKADEDVRMISAEAPVVFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAAVTRTDIFDFLVDIVP 157 89****99999************************************************************************************* PP NF-YC 98 rdel 101 r++l RrC264_p4 158 REDL 161 *986 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47113	4.19E-31	52	152	IPR009072	Histone-fold
Gene3D	G3DSA:1.10.20.10	5.6E-37	72	144	IPR009072	Histone-fold
Pfam	PF00808	4.4E-21	80	143	IPR003958	Transcription factor CBF/NF-Y/archaeal histone domain
Gene3D	G3DSA:3.30.200.20	5.9E-35	269	402	No hit	No description
CDD	cd06606	9.47E-165	288	551	No hit	No description
SuperFamily	SSF56112	1.35E-86	289	581	IPR011009	Protein kinase-like domain
SMART	SM00220	5.1E-98	289	551	IPR000719	Protein kinase domain
PROSITE profile	PS50011	52.47	289	551	IPR000719	Protein kinase domain
Pfam	PF00069	2.4E-73	290	551	IPR000719	Protein kinase domain
PROSITE pattern	PS00107	0	295	318	IPR017441	Protein kinase, ATP binding site
Gene3D	G3DSA:1.10.510.10	3.6E-53	403	557	No hit	No description
PROSITE pattern	PS00108	0	413	425	IPR008271	Serine/threonine-protein kinase, active site

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006979	Biological Process	response to oxidative stress
GO:0009908	Biological Process	flower development
GO:0046777	Biological Process	protein autophosphorylation
GO:0004674	Molecular Function	protein serine/threonine kinase activity
GO:0005524	Molecular Function	ATP binding
GO:0046982	Molecular Function	protein heterodimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 872 aa Download sequence Send to blast
MDQQEHGQSG AMNYGANPYQ TNPMTTNVAG SAGPAAPQGQ LAFHQIHQKQ QQQQLTQQLQ 60 VFWENQFKEI EKTTDFKNHS LPLARIKKIM KADEDVRMIS AEAPVVFARA CEMFILELTL 120 RSWNHTEENK RRTLQKNDIA AAVTRTDIFD FLVDIVPRED LRDEVLGRGT VPEAAAAGYP 180 YGYLPPGTGP IGNPGMVMGN PGGAYPPNPV KTKPPKSIEM QDFFGSVRRS LVFRPPAGDE 240 DNNQENQPPF PGVLADKITS CIRKSRALIK PSSSPPPPTT VDTPPPISWR KGQLIGRGAF 300 GTVYMGMNLD SGELLAVKQV LIASNYASKE KTQAHIQELE EEVKLLKNLS HPNIVRYLGT 360 VREDDTLNIL LEFVPGGSIS SLLEKFGPFP ESVVRTYTKQ LLFGLEYLHN HAIMHRDIKG 420 ANILVDNKGC IKLADFGASK QVAELATISG AKSMKGTPYW MAPEVILQTG HSFSADIWSV 480 GCTVIEMVTG KAPWSQQYKE VAAIFFIGTT KSHPPIPDTL SSDAKDFLLK CLQEEPKMRP 540 TASELLKHPF VTGQHKESAS TDLVSIMNNG STPTLSELTN TKCSPHSTCN DVGDMCNLGS 600 LNYSLASPFK SIQNNNFRQQ NDNGGEEDDM CLIDDDNFLT FNGESGPILK NNSDLKKSCD 660 ATSDISNALN AKFDESPCNG EKETKMSMEV DQPSYSEDDE ELTETKIKSF LDEKAAELKK 720 LQTPLYEEYY NNLITCSPSG MESNLSSSKR EDTARGFLKL PPKSRSPSRG PLGGSPSRAT 780 DTVSCTKSPG SGSSREVNMN DGGDEASQDT EEESKNQSAA LSEIEKKWKE ELDQELERKR 840 QEIMRQAGMG SSPRDRSLSR HREKSRFASP GK

Sequence ? help Back to Top

Protein Sequence Length: 872 aa Download sequence Send to blast

MDQQEHGQSG AMNYGANPYQ TNPMTTNVAG SAGPAAPQGQ LAFHQIHQKQ QQQQLTQQLQ  60
VFWENQFKEI EKTTDFKNHS LPLARIKKIM KADEDVRMIS AEAPVVFARA CEMFILELTL  120
RSWNHTEENK RRTLQKNDIA AAVTRTDIFD FLVDIVPRED LRDEVLGRGT VPEAAAAGYP  180
YGYLPPGTGP IGNPGMVMGN PGGAYPPNPV KTKPPKSIEM QDFFGSVRRS LVFRPPAGDE  240
DNNQENQPPF PGVLADKITS CIRKSRALIK PSSSPPPPTT VDTPPPISWR KGQLIGRGAF  300
GTVYMGMNLD SGELLAVKQV LIASNYASKE KTQAHIQELE EEVKLLKNLS HPNIVRYLGT  360
VREDDTLNIL LEFVPGGSIS SLLEKFGPFP ESVVRTYTKQ LLFGLEYLHN HAIMHRDIKG  420
ANILVDNKGC IKLADFGASK QVAELATISG AKSMKGTPYW MAPEVILQTG HSFSADIWSV  480
GCTVIEMVTG KAPWSQQYKE VAAIFFIGTT KSHPPIPDTL SSDAKDFLLK CLQEEPKMRP  540
TASELLKHPF VTGQHKESAS TDLVSIMNNG STPTLSELTN TKCSPHSTCN DVGDMCNLGS  600
LNYSLASPFK SIQNNNFRQQ NDNGGEEDDM CLIDDDNFLT FNGESGPILK NNSDLKKSCD  660
ATSDISNALN AKFDESPCNG EKETKMSMEV DQPSYSEDDE ELTETKIKSF LDEKAAELKK  720
LQTPLYEEYY NNLITCSPSG MESNLSSSKR EDTARGFLKL PPKSRSPSRG PLGGSPSRAT  780
DTVSCTKSPG SGSSREVNMN DGGDEASQDT EEESKNQSAA LSEIEKKWKE ELDQELERKR  840
QEIMRQAGMG SSPRDRSLSR HREKSRFASP GK

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4bf2_A	2e-61	294	579	42	323	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
4bf2_B	2e-61	294	579	42	323	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
4bhn_A	2e-61	294	579	42	323	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
4bhn_B	2e-61	294	579	42	323	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
4bib_A	1e-61	294	579	26	307	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
4bib_B	1e-61	294	579	26	307	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
4bic_A	1e-61	294	579	26	307	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
4bic_B	1e-61	294	579	26	307	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
4bid_A	1e-61	294	579	26	307	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
4bid_B	1e-61	294	579	26	307	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
4bie_A	1e-61	294	579	26	307	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
4bie_B	1e-61	294	579	26	307	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	130	136	RRTLQKN

Functional Description ? help Back to Top
Source	Description
UniProt	May be involved in an oxidative stress-mediated signaling cascade that phosphorylates downstream MAP kinases MPK3 and MPK6. May suppress auxin signaling that promotes cell cycle. Functionally redundant to ANP2 and ANP3 in the positive regulation of cytokinesis. {ECO:0000269\|PubMed:10717008, ECO:0000269\|PubMed:12034900}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KM083105	0.0	KM083105.1 Brassica napus cultivar DH12075 mitogen-activated protein kinase kinase kinase 1.1 (MAPKKK1) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_018435510.1	0.0	PREDICTED: mitogen-activated protein kinase kinase kinase ANP1
Swissprot	O22040	0.0	ANP1_ARATH; Mitogen-activated protein kinase kinase kinase ANP1
TrEMBL	A0A0U1XJX7	0.0	A0A0U1XJX7_BRANA; Mitogen-activated protein kinase kinase kinase 1.1
TrEMBL	A0A3P6F166	0.0	A0A3P6F166_BRAOL; Uncharacterized protein
STRING	Bra018588.1-P	0.0	(Brassica rapa)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G08970.4	2e-86	nuclear factor Y, subunit C9

Publications ? help Back to Top
Kovtun Y,Chiu WL,Tena G,Sheen J Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proc. Natl. Acad. Sci. U.S.A., 2000. 97(6): p. 2940-5 [PMID:10717008] Krysan PJ,Jester PJ,Gottwald JR,Sussman MR An Arabidopsis mitogen-activated protein kinase kinase kinase gene family encodes essential positive regulators of cytokinesis. Plant Cell, 2002. 14(5): p. 1109-20 [PMID:12034900] Lukowitz W,Roeder A,Parmenter D,Somerville C A MAPKK kinase gene regulates extra-embryonic cell fate in Arabidopsis. Cell, 2004. 116(1): p. 109-19 [PMID:14718171] Takahashi Y,Soyano T,Kosetsu K,Sasabe M,Machida Y HINKEL kinesin, ANP MAPKKKs and MKK6/ANQ MAPKK, which phosphorylates and activates MPK4 MAPK, constitute a pathway that is required for cytokinesis in Arabidopsis thaliana. Plant Cell Physiol., 2010. 51(10): p. 1766-76 [PMID:20802223] Sasabe M,Machida Y Regulation of organization and function of microtubules by the mitogen-activated protein kinase cascade during plant cytokinesis. Cytoskeleton (Hoboken), 2012. 69(11): p. 913-8 [PMID:23027702] Christian M,Qi Y,Zhang Y,Voytas DF Targeted mutagenesis of Arabidopsis thaliana using engineered TAL effector nucleases. G3 (Bethesda), 2013. 3(10): p. 1697-705 [PMID:23979944] Savatin DV, et al. The Arabidopsis NUCLEUS- AND PHRAGMOPLAST-LOCALIZED KINASE1-Related Protein Kinases Are Required for Elicitor-Induced Oxidative Burst and Immunity. Plant Physiol., 2014. 165(3): p. 1188-1202 [PMID:24812107] Su SH,Krysan PJ A double-mutant collection targeting MAP kinase related genes in Arabidopsis for studying genetic interactions. Plant J., 2016. 88(5): p. 867-878 [PMID:27490954] Gigli Bisceglia N,Savatin DV,Cervone F,Engelsdorf T,De Lorenzo G Loss of the Arabidopsis Protein Kinases ANPs Affects Root Cell Wall Composition, and Triggers the Cell Wall Damage Syndrome. Front Plant Sci, 2017. 8: p. 2234 [PMID:29403509] Nishihama R,Banno H,Kawahara E,Irie K,Machida Y Possible involvement of differential splicing in regulation of the activity of Arabidopsis ANP1 that is related to mitogen-activated protein kinase kinase kinases (MAPKKKs). Plant J., 1997. 12(1): p. 39-48 [PMID:9263451]