PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Pgl016155

Organism

Picea glauca

Taxonomic ID

3330

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Acrogymnospermae; Pinidae; Pinales; Pinaceae; Picea

Family

bHLH

Protein Properties

Length: 395aa MW: 43976.6 Da PI: 7.629

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
gnl\|UG\|Pgl#S55334526	PU_ref	Unigene	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	40.6	4.7e-13	142	193	1	55
CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +r +h e+Er+RR+++N ++ Lr+l+P++ + ++ + a+i A++ +k+L+ Pgl016155 142 QRITHIEVERNRRKQMNAHLDVLRSLIPQS---YTQRGDQASIIGGAIDLVKELE 193 799*************************...9***************9995 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47459	3.14E-15	134	202	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	14.818	141	192	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	6.38E-12	141	197	No hit	No description
Gene3D	G3DSA:4.10.280.10	1.5E-12	142	196	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	2.7E-10	142	193	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	1.3E-9	147	198	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 395 aa Download sequence Send to blast
MEIIEEMIEI PRLNDNLGLN IPWLHGGLFR KRLTSSPLHS EPAGKQGNTG PNLENYFTRL 60 VQGFEPVESC VTALSELKPG GNIDTFNAVH SHSDTAEDGI SDTTQEDQQN AISMAVPAAD 120 AGKGRKRRRL KVSKNSEEVL SQRITHIEVE RNRRKQMNAH LDVLRSLIPQ SYTQRGDQAS 180 IIGGAIDLVK ELEQILQSLQ FQKIKRENED RTDPKNCLSI LPPHQLSVPC NSKCVVDTSS 240 SRLREAEDKS SIADIEVTLI ETHGSLKILS KKKHGQLLKI VTALEGFHMT ILHLNITSMD 300 EAVLFSFNVK VEDECPLTSA NDXSKITSPN FENSACMLVI GPFIENARLL ARSTQQQLYR 360 KQGNWSTARV AYQFKAKIAS RANANGLNYS SHNVV

Sequence ? help Back to Top

Protein Sequence Length: 395 aa Download sequence Send to blast

MEIIEEMIEI PRLNDNLGLN IPWLHGGLFR KRLTSSPLHS EPAGKQGNTG PNLENYFTRL  60
VQGFEPVESC VTALSELKPG GNIDTFNAVH SHSDTAEDGI SDTTQEDQQN AISMAVPAAD  120
AGKGRKRRRL KVSKNSEEVL SQRITHIEVE RNRRKQMNAH LDVLRSLIPQ SYTQRGDQAS  180
IIGGAIDLVK ELEQILQSLQ FQKIKRENED RTDPKNCLSI LPPHQLSVPC NSKCVVDTSS  240
SRLREAEDKS SIADIEVTLI ETHGSLKILS KKKHGQLLKI VTALEGFHMT ILHLNITSMD  300
EAVLFSFNVK VEDECPLTSA NDXSKITSPN FENSACMLVI GPFIENARLL ARSTQQQLYR  360
KQGNWSTARV AYQFKAKIAS RANANGLNYS SHNVV

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	124	128	RKRRR
2	125	129	KRRRL

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Pgl.6613	0.0	cone\| somatic embryo\| stem

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Not expressed in meristemoids, but strongly expressed in guard mother cells (GMCs) and in young guard cells (at protein level) (PubMed:17088607). Expressed at the transition to terminal stomatal differentiation, just before and after the symmetric division of stomatal differentiation, being confined to late-stage GMC and to young, still differentiating guard cells (PubMed:24571519). {ECO:0000269\|PubMed:17088607, ECO:0000269\|PubMed:24571519}.
Uniprot	TISSUE SPECIFICITY: Resctricted to stomatal cell lineages (at protein level). Expressed in roots, leaves, stems, and flowers. {ECO:0000269\|PubMed:12679534, ECO:0000269\|PubMed:17088607}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator (PubMed:17088607, PubMed:17183265, PubMed:17183267). Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity (PubMed:24571519). Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata (PubMed:17088607, PubMed:17183265, PubMed:17183267). Prevents histone H3K27me3 marks and derepresses stem cell gene expression (PubMed:24654956). {ECO:0000269\|PubMed:17088607, ECO:0000269\|PubMed:17183265, ECO:0000269\|PubMed:17183267, ECO:0000269\|PubMed:24571519, ECO:0000269\|PubMed:24654956}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Inhibited by low relative humidity (LRH) via epigenetic CG methylation, thus leading to a reduced stomatal index. {ECO:0000269\|PubMed:22442411}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_010272971.1	2e-63	PREDICTED: transcription factor bHLH96
Swissprot	Q56YJ8	2e-53	FAMA_ARATH; Transcription factor FAMA
TrEMBL	B8LLL6	3e-70	B8LLL6_PICSI; Uncharacterized protein
STRING	XP_010272971.1	7e-63	(Nelumbo nucifera)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G24140.1	1e-53	bHLH family protein

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Matos JL, et al. Irreversible fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RETINOBLASTOMA-RELATED module. Elife, 2015. [PMID:25303364] Chen L, et al. NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development, 2016. 143(9): p. 1600-11 [PMID:26989174] Lee JH,Jung JH,Park CM Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis. Plant Cell, 2017. 29(11): p. 2817-2830 [PMID:29070509] Han X, et al. Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons. Plant Physiol., 2018. 176(4): p. 2871-2885 [PMID:29496884] Li X,Yang R,Chen H The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA. PLoS ONE, 2018. 13(3): p. e0193458 [PMID:29513733]