PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID LOC_Os02g08440.3
Common NameOsJ_05628
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa
Family WRKY
Protein Properties Length: 232aa    MW: 24961.4 Da    PI: 8.3674
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
LOC_Os02g08440.3genomeMSUView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY61.12.1e-19192230138
                       ---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEE CS
              WRKY   1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkv 38 
                       ++Dgy+WrKYGqK+ k++++pr+Y+rC++a  Cpvkkkv
  LOC_Os02g08440.3 192 VKDGYQWRKYGQKVTKDNPCPRAYFRCSFApACPVKKKV 230
                       58***************************99*******9 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5081117.523187231IPR003657WRKY domain
SuperFamilySSF1182902.09E-16191230IPR003657WRKY domain
Gene3DG3DSA:2.20.25.802.8E-18191230IPR003657WRKY domain
SMARTSM007749.2E-9192231IPR003657WRKY domain
PfamPF031062.6E-15193230IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0002237Biological Processresponse to molecule of bacterial origin
GO:0002238Biological Processresponse to molecule of fungal origin
GO:0009409Biological Processresponse to cold
GO:0009414Biological Processresponse to water deprivation
GO:0009611Biological Processresponse to wounding
GO:0009623Biological Processresponse to parasitic fungus
GO:0009651Biological Processresponse to salt stress
GO:0009685Biological Processgibberellin metabolic process
GO:0009723Biological Processresponse to ethylene
GO:0009737Biological Processresponse to abscisic acid
GO:0009751Biological Processresponse to salicylic acid
GO:0009753Biological Processresponse to jasmonic acid
GO:0009938Biological Processnegative regulation of gibberellic acid mediated signaling pathway
GO:0010200Biological Processresponse to chitin
GO:0031347Biological Processregulation of defense response
GO:0042742Biological Processdefense response to bacterium
GO:0045892Biological Processnegative regulation of transcription, DNA-templated
GO:0050691Biological Processregulation of defense response to virus by host
GO:0050832Biological Processdefense response to fungus
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
GO:1990841Molecular Functionpromoter-specific chromatin binding
Sequence ? help Back to Top
Protein Sequence    Length: 232 aa     Download sequence    Send to blast
MDPWISTQPS LSLDLRVGLP ATAAVAMVKP KVLVEEDFFH QQPLKKDPEV AALEAELKRM  60
GAENRQLSEM LAAVAAKYEA LQSQFSDMVT ASANNGGGGG NNPSSTSEGG SVSPSRKRKS  120
ESLDDSPPPP PPPHPHAAPH HMHVMPGAAA AGYADQTECT SGEPCKRIRE ECKPKISKLY  180
VHADPSDLSL VVKDGYQWRK YGQKVTKDNP CPRAYFRCSF APACPVKKKV T*
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1114119SRKRKS
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Os.120320.0callus| flower| leaf| panicle| root| seed| stem
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1166416240.0
Expression AtlasA3A3T5-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Highly expressed in aleurone cells (PubMed:15047897, PubMed:16623886). In seeds, predominantly present in the plumule, radicle and scutellum of the embryo (PubMed:16623886). Expressed in roots, stems, young leaves and spikelets (PubMed:16919842). {ECO:0000269|PubMed:15047897, ECO:0000269|PubMed:16623886, ECO:0000269|PubMed:16919842}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription repressor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:18175928, PubMed:15047897, PubMed:16623886). Represses specifically gibberellic acid (GA)-induced promoters in aleurone cells, probably by interfering with GAM1 (PubMed:15047897, PubMed:16623886, PubMed:19199048). Regulates, probably indirectly, the activation of defense-related genes such as GF14E during defense response (PubMed:18175928, PubMed:26851365, PubMed:26467468). Modulates plant innate immunity against X.oryzae pv. oryzae (Xoo) (PubMed:21961049). Confers resistance to the virulent bacterial pathogen Xoo 13751, probably via the regulation of NPR1 and PR1b defense signaling pathways (PubMed:16919842). {ECO:0000269|PubMed:15047897, ECO:0000269|PubMed:16623886, ECO:0000269|PubMed:16919842, ECO:0000269|PubMed:18175928, ECO:0000269|PubMed:19199048, ECO:0000269|PubMed:21961049, ECO:0000269|PubMed:26467468, ECO:0000269|PubMed:26851365}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00251DAPTransfer from AT1G80840Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapLOC_Os02g08440.3
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced by biotic elicitors (e.g. fungal chitin oligosaccharide and fungal cerebroside elicitors) and pathogen infection (e.g. the compatible pathogenic fungus M.grisea race 007, M. grisea crabgrass BR29) (PubMed:18175928, PubMed:19772648, PubMed:23462973). Accumulates in response to M.oryzae (PubMed:21726398). Triggered by defense signaling molecules, such as salicylic acid (SA), methyl jasmonate (MeJA), 1-aminocyclo-propane-1-carboxylic acid (ACC), wounding and pathogen infection (e.g. X.oryzae) (PubMed:16919842). Repressed by gibberellic acid (GA) (at protein level) (PubMed:15047897, PubMed:19199048). Induced by abscisic acid (ABA) in aleurone cells, roots and leaves (PubMed:15618416, PubMed:16623886). Accumulates in response to uniconazole, a GA biosynthesis inhibitor (PubMed:16623886). Triggered strongly by cold in leaves, stems and developing spikes, but moderately by drought and salt stresses (PubMed:14645724, PubMed:23495849). {ECO:0000269|PubMed:14645724, ECO:0000269|PubMed:15047897, ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:16623886, ECO:0000269|PubMed:16919842, ECO:0000269|PubMed:18175928, ECO:0000269|PubMed:19199048, ECO:0000269|PubMed:19772648, ECO:0000269|PubMed:21726398, ECO:0000269|PubMed:23462973, ECO:0000269|PubMed:23495849}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Phenotype -- Mutation ? help Back to Top
Source ID
RiceGEOs02g08440
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAB1908170.0AB190817.1 Oryza sativa Japonica Group OsWRKY71 mRNA for transcription factor OsWRKY71, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_015627417.11e-170WRKY transcription factor WRKY71
SwissprotQ6QHD11e-171WRK71_ORYSJ; WRKY transcription factor WRKY71
TrEMBLA0A0D9YNA91e-169A0A0D9YNA9_9ORYZ; Uncharacterized protein
TrEMBLA0A0E0NAS61e-169A0A0E0NAS6_ORYRU; Uncharacterized protein
TrEMBLA3A3T51e-169A3A3T5_ORYSJ; Uncharacterized protein
STRINGOGLUM02G06150.11e-170(Oryza glumipatula)
STRINGORUFI02G06290.11e-170(Oryza rufipogon)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT1G80840.13e-34WRKY DNA-binding protein 40
Publications ? help Back to Top
  1. Rabbani MA, et al.
    Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses.
    Plant Physiol., 2003. 133(4): p. 1755-67
    [PMID:14645724]
  2. Zhang Y,Wang L
    The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants.
    BMC Evol. Biol., 2005. 5: p. 1
    [PMID:15629062]
  3. Wu KL,Guo ZJ,Wang HH,Li J
    The WRKY family of transcription factors in rice and Arabidopsis and their origins.
    DNA Res., 2005. 12(1): p. 9-26
    [PMID:16106749]
  4. Xie Z, et al.
    Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells.
    Plant J., 2006. 46(2): p. 231-42
    [PMID:16623886]
  5. Zou X,Neuman D,Shen QJ
    Interactions of two transcriptional repressors and two transcriptional activators in modulating gibberellin signaling in aleurone cells.
    Plant Physiol., 2008. 148(1): p. 176-86
    [PMID:18621977]
  6. Zhang ZL, et al.
    A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells.
    Plant Mol. Biol., 2009. 70(1-2): p. 139-51
    [PMID:19199048]
  7. Berri S, et al.
    Characterization of WRKY co-regulatory networks in rice and Arabidopsis.
    BMC Plant Biol., 2009. 9: p. 120
    [PMID:19772648]
  8. Delteil A, et al.
    Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence.
    Mol. Plant Pathol., 2012. 13(1): p. 72-82
    [PMID:21726398]
  9. Peng Y,Bartley LE,Canlas P,Ronald PC
    OsWRKY IIa Transcription Factors Modulate Rice Innate Immunity.
    Rice (N Y), 2010. 3(1): p. 36-42
    [PMID:21961049]
  10. Chujo T, et al.
    OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate immune responses in rice against rice blast fungus.
    Plant Mol. Biol., 2013. 82(1-2): p. 23-37
    [PMID:23462973]
  11. Kovalchuk N, et al.
    Optimization of TaDREB3 gene expression in transgenic barley using cold-inducible promoters.
    Plant Biotechnol. J., 2013. 11(6): p. 659-70
    [PMID:23495849]
  12. Basu S,Roychoudhury A
    Expression profiling of abiotic stress-inducible genes in response to multiple stresses in rice (Oryza sativa L.) varieties with contrasting level of stress tolerance.
    Biomed Res Int, 2014. 2014: p. 706890
    [PMID:25110688]
  13. Liu Q, et al.
    OsGF14b Positively Regulates Panicle Blast Resistance but Negatively Regulates Leaf Blast Resistance in Rice.
    Mol. Plant Microbe Interact., 2016. 29(1): p. 46-56
    [PMID:26467468]
  14. Liu Q, et al.
    OsGF14e positively regulates panicle blast resistance in rice.
    Biochem. Biophys. Res. Commun., 2016. 471(1): p. 247-52
    [PMID:26851365]