PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Medtr7g085810.1
Common NameERN, MtrDRAFT_AC170580g4v1
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Trifolieae; Medicago
Family ERF
Protein Properties Length: 260aa    MW: 28842 Da    PI: 5.8228
Description ERF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Medtr7g085810.1genomeMtView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP258.22.1e-182978255
              AP2  2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55
                     ++ GVr+++ +grWvAeI+d +   ++ r +lg+f taeeAa+a+++a+  l+g
  Medtr7g085810.1 29 KFVGVRQRP-SGRWVAEIKDTT---QKIRMWLGTFETAEEAARAYDEAACLLRG 78
                     799******.7*********32...35**********************99987 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5103219.5852986IPR001471AP2/ERF domain
PfamPF008474.9E-132978IPR001471AP2/ERF domain
CDDcd000182.46E-322985No hitNo description
SuperFamilySSF541712.94E-192986IPR016177DNA-binding domain
Gene3DG3DSA:3.30.730.106.2E-282986IPR001471AP2/ERF domain
SMARTSM003801.6E-352992IPR001471AP2/ERF domain
PRINTSPR003678.5E-83041IPR001471AP2/ERF domain
PRINTSPR003678.5E-85268IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0000302Biological Processresponse to reactive oxygen species
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009723Biological Processresponse to ethylene
GO:0035865Biological Processcellular response to potassium ion
GO:0048528Biological Processpost-embryonic root development
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 260 aa     Download sequence    Send to blast
MQNQKAGISV TNKGGKFKGR NRNSNNTNKF VGVRQRPSGR WVAEIKDTTQ KIRMWLGTFE  60
TAEEAARAYD EAACLLRGSN TRTNFITHVS LDSPLASRIR NLLNNRKGDK KQEDGAVASA  120
PSNSKTTISN TSTITSNDDN KESTLSTCAT RNTELFEDAY KPDLSNCKEV FESGSQSNIS  180
CGFGPFFDHF SFTQLLDMAK NDDITDASSL ELSEFERMKV ERQISASLYA INGVHEYMET  240
VQESNEALWD LPPLCSLFC*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5wx9_A2e-1428951381Ethylene-responsive transcription factor ERF096
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Mtr.113880.0root
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in roots, root hairs and leaves (PubMed:17827349). Expressed in root epidermis and root hairs (PubMed:23077241). {ECO:0000269|PubMed:17827349, ECO:0000269|PubMed:23077241}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor involved in symbiotic nodule signaling in response to rhizobial Nod factors (NFs) (PubMed:17449807, PubMed:17827349). Binds to the GCC-box (NF-responsive box) of ENOD11 promoter. Acts as transcriptional activator of NF-responsive box-containing target gene promoters in root hairs (PubMed:17827349). Functions as a transcriptional regulator required for root infection by symbiotic rhizobia, infection thread (IT) formation and maintenance, and nodule development. Necessary for NF-induced gene expression and spontaneous nodulation activated by CCAMK. Functions downstream of CCAMK to activate nodulation gene expression (PubMed:17449807). Involved in early stages of root nodule development. Functions redundantly with ERN2. Is essential with ERN2 for the initiation of root hair infection, and nodule organogenesis and development. Required for accurate expression of the NF signaling genes ENOD11 and ENOD12 (PubMed:23077241, PubMed:27208242). {ECO:0000269|PubMed:17449807, ECO:0000269|PubMed:17827349, ECO:0000269|PubMed:23077241, ECO:0000269|PubMed:27208242}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00521DAPTransfer from AT5G19790Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapMedtr7g085810.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced in roots from 1 to 21 day after inoculation with Sinorhizobium meliloti (PubMed:17449807). Induced by Nod factors in root hairs (PubMed:17827349). {ECO:0000269|PubMed:17449807, ECO:0000269|PubMed:17827349}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAC1705800.0AC170580.3 Medicago truncatula chromosome 7 clone mte1-32m6, complete sequence.
GenBankDQ9849420.0DQ984942.1 Medicago truncatula Ser/Thr protein kinase (PRK), exostosin-like protein (EXO), putative RNA binding protein (RAN BP), transcription factor ERF (ERN), and alpha-tubulin (aTUB) genes, complete cds; and unknown gene.
GenBankEF3963300.0EF396330.1 Medicago truncatula ERF transcription factor (ERN) gene, complete cds.
GenBankEU0388020.0EU038802.2 Medicago truncatula ERN1 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_003624634.30.0ethylene-responsive transcription factor RAP2-11
SwissprotA2Q5W10.0ERN1_MEDTR; Ethylene-responsive transcription factor ERN1
TrEMBLA0A396H8I10.0A0A396H8I1_MEDTR; Putative transcription factor AP2-EREBP family
STRINGAES808520.0(Medicago truncatula)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
FabidsOGEF33413069
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G19790.11e-38related to AP2 11
Publications ? help Back to Top
  1. Middleton PH, et al.
    An ERF transcription factor in Medicago truncatula that is essential for Nod factor signal transduction.
    Plant Cell, 2007. 19(4): p. 1221-34
    [PMID:17449807]
  2. Andriankaja A, et al.
    AP2-ERF transcription factors mediate Nod factor dependent Mt ENOD11 activation in root hairs via a novel cis-regulatory motif.
    Plant Cell, 2007. 19(9): p. 2866-85
    [PMID:17827349]
  3. Cerri MR, et al.
    Medicago truncatula ERN transcription factors: regulatory interplay with NSP1/NSP2 GRAS factors and expression dynamics throughout rhizobial infection.
    Plant Physiol., 2012. 160(4): p. 2155-72
    [PMID:23077241]
  4. Tang H, et al.
    An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
    BMC Genomics, 2014. 15: p. 312
    [PMID:24767513]
  5. Cerri MR, et al.
    The Symbiosis-Related ERN Transcription Factors Act in Concert to Coordinate Rhizobial Host Root Infection.
    Plant Physiol., 2016. 171(2): p. 1037-54
    [PMID:27208242]