PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Manes.04G072000.1.p

Organism

Manihot esculenta

Taxonomic ID

3983

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Manihoteae; Manihot

Family

bHLH

Protein Properties

Length: 587aa MW: 66250.7 Da PI: 5.5914

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Manes.04G072000.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	35.1	2.4e-11	342	384	8	55
HHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHHHHH CS HLH 8 rErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksLq 55 +ErrRR+r+N ++ Lr l+Pk + l+Ka+iL A+e++k+Lq Manes.04G072000.1.p 342 AERRRRKRLNGRLYDLRALVPKiS------NLNKAAILGDAIEFVKELQ 384 7*******************66......8***************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	1.4E-23	9	157	IPR025610	Transcription factor MYC/MYB N-terminal
PROSITE profile	PS50888	13.579	334	383	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	8.24E-15	338	397	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	1.6E-12	340	389	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	3.1E-14	342	397	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	8.4E-9	342	384	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	5.71E-5	348	386	No hit	No description
CDD	cd04873	0.00221	470	533	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009555	Biological Process	pollen development
GO:0048657	Biological Process	anther wall tapetum cell differentiation
GO:0005634	Cellular Component	nucleus
GO:0000978	Molecular Function	RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 587 aa Download sequence Send to blast
MIVFQDSMER LRPLVGLKGW DYCVLWKLSD DQRFLEWIDC CCGGNENMQN GAEELQFPVS 60 SSSAMACRDV IFQHPRTKSC ELLAQLPSSM PLDSGVHAQT LLSNQPRWLN FSNSCDSSVL 120 EGTVGTRALI PVAGGIIELF VTKQVSEDQN VINFITSQCS ILMEQEAIIN STNMETGFSA 180 NVNMISEQQS RPFIADGHDT EHKDHQMNQF QAPVSPATAL ENLNLPYDIS ADRIHLCDMK 240 FLQQFNYNDQ ENKNKNDMFF EGVQDMDALQ KSMVMNNTEN MHMKFTESSA NKEQQGNDKD 300 SVKQENGRSD SISDCSDQND DENDAKYRRR PGRGPQAKNL FAERRRRKRL NGRLYDLRAL 360 VPKISNLNKA AILGDAIEFV KELQKQAKEL QDELEEHSDD ERAKNGNHNN NIPQEILNQN 420 GGFVNGFDVG ASEVSCSKLN HKASEISHDK GQQMEVQVEV AQIDGNEFFV KVFCEHKPGG 480 FVRLMEALDS LGLEVTNANV TSFRGLVSNV LKVEKKDSEM VQADYVRDSL LELTRDPPTG 540 IWSEMAKASE NGSGMDYHHH QHHHHHNHHH QLHNGHVNSN HHHLHS*

Sequence ? help Back to Top

Protein Sequence Length: 587 aa Download sequence Send to blast

MIVFQDSMER LRPLVGLKGW DYCVLWKLSD DQRFLEWIDC CCGGNENMQN GAEELQFPVS  60
SSSAMACRDV IFQHPRTKSC ELLAQLPSSM PLDSGVHAQT LLSNQPRWLN FSNSCDSSVL  120
EGTVGTRALI PVAGGIIELF VTKQVSEDQN VINFITSQCS ILMEQEAIIN STNMETGFSA  180
NVNMISEQQS RPFIADGHDT EHKDHQMNQF QAPVSPATAL ENLNLPYDIS ADRIHLCDMK  240
FLQQFNYNDQ ENKNKNDMFF EGVQDMDALQ KSMVMNNTEN MHMKFTESSA NKEQQGNDKD  300
SVKQENGRSD SISDCSDQND DENDAKYRRR PGRGPQAKNL FAERRRRKRL NGRLYDLRAL  360
VPKISNLNKA AILGDAIEFV KELQKQAKEL QDELEEHSDD ERAKNGNHNN NIPQEILNQN  420
GGFVNGFDVG ASEVSCSKLN HKASEISHDK GQQMEVQVEV AQIDGNEFFV KVFCEHKPGG  480
FVRLMEALDS LGLEVTNANV TSFRGLVSNV LKVEKKDSEM VQADYVRDSL LELTRDPPTG  540
IWSEMAKASE NGSGMDYHHH QHHHHHNHHH QLHNGHVNSN HHHLHS*

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	327	348	RRRPGRGPQAKNLFAERRRRKR
2	342	349	ERRRRKRL

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther (PubMed:12535353). Binds E-box regions in the AHL16/TEK promoter. {ECO:0000269\|PubMed:12535353, ECO:0000269\|PubMed:24804694}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Manes.04G072000.1.p

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_021611257.1	0.0	transcription factor ABORTED MICROSPORES
Swissprot	Q9ZVX2	1e-160	AMS_ARATH; Transcription factor ABORTED MICROSPORES
TrEMBL	A0A251L277	0.0	A0A251L277_MANES; Uncharacterized protein
STRING	cassava4.1_029776m	0.0	(Manihot esculenta)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF4225	32	47

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G16910.1	1e-156	bHLH family protein

Link Out ? help Back to Top
Phytozome	Manes.04G072000.1.p

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Jiang J,Zhang Z,Cao J Pollen wall development: the associated enzymes and metabolic pathways. Plant Biol (Stuttg), 2013. 15(2): p. 249-63 [PMID:23252839] Xu J, et al. ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis. Plant Cell, 2014. 26(4): p. 1544-1556 [PMID:24781116] Qian H, et al. Trace concentrations of imazethapyr (IM) affect floral organs development and reproduction in Arabidopsis thaliana: IM-induced inhibition of key genes regulating anther and pollen biosynthesis. Ecotoxicology, 2015. 24(1): p. 163-71 [PMID:25348600] Xiong SX, et al. The transcription factors MS188 and AMS form a complex to activate the expression of CYP703A2 for sporopollenin biosynthesis in Arabidopsis thaliana. Plant J., 2016. 88(6): p. 936-946 [PMID:27460657] Ferguson AC, et al. Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis. New Phytol., 2017. 213(2): p. 778-790 [PMID:27787905] Lou Y, et al. Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for anther development in Arabidopsis thaliana. New Phytol., 2018. 217(1): p. 378-391 [PMID:28940573] Bi H,Fan W,Zhang P C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis. Front Plant Sci, 2017. 8: p. 1689 [PMID:29021807]