PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID MDP0000708692
Common NameWRKY9
Organism
Malus domestica
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Maloideae; Maleae; Malus
Family WRKY
Protein Properties Length: 521aa    MW: 57709 Da    PI: 7.5962
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
MDP0000708692genomeGDRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY105.82.3e-33221278260
                    --SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS
           WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 
                    dDg+nWrKYGqK+vkgse+prsYY+Ct+++Cp+kkkvers  d+++++i+Y+g+Hnh+k
  MDP0000708692 221 DDGFNWRKYGQKQVKGSENPRSYYKCTFPNCPTKKKVERSL-DGQITQIVYKGSHNHPK 278
                    8****************************************.***************85 PP
2WRKY106.11.8e-33385443159
                    ---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS
           WRKY   1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 
                    ldDgy+WrKYGqK+vkg+++prsYY+Cts gCpv+k+ver+++d+k+v++tYeg+Hnh+
  MDP0000708692 385 LDDGYRWRKYGQKVVKGNPNPRSYYKCTSVGCPVRKHVERASHDTKAVITTYEGKHNHD 443
                    59********************************************************7 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5081123.548215279IPR003657WRKY domain
Gene3DG3DSA:2.20.25.802.8E-28215279IPR003657WRKY domain
SuperFamilySSF1182905.89E-25219279IPR003657WRKY domain
SMARTSM007742.8E-36220278IPR003657WRKY domain
PfamPF031062.4E-25221277IPR003657WRKY domain
Gene3DG3DSA:2.20.25.801.4E-37370445IPR003657WRKY domain
SuperFamilySSF1182902.09E-29377445IPR003657WRKY domain
PROSITE profilePS5081138.802380445IPR003657WRKY domain
SMARTSM007743.2E-39385444IPR003657WRKY domain
PfamPF031061.2E-25386443IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009409Biological Processresponse to cold
GO:0009414Biological Processresponse to water deprivation
GO:0009651Biological Processresponse to salt stress
GO:0010120Biological Processcamalexin biosynthetic process
GO:0010200Biological Processresponse to chitin
GO:0010508Biological Processpositive regulation of autophagy
GO:0042742Biological Processdefense response to bacterium
GO:0050832Biological Processdefense response to fungus
GO:0070370Biological Processcellular heat acclimation
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 521 aa     Download sequence    Send to blast
MTSSFTNLLT SNMENNGIGQ DRTNWGGQFS DYSSNPNRFT DRNGTEIPKF RSLQPPSLPL  60
SPPPVSPSSY LASTPAFSPT DFFSSPMFLS SSNNLESPTT GAFSSQIFDW VNNSKDTQQG  120
IRESEPKMFS DFSFQPESRP ATNLQSASSM VSVEEPFKRE RQAWDFSTTR QADSSAEKTG  180
VKSEFEPIEA NTQSNGLNGA PKSDYLHSTQ SSQYGREQKS DDGFNWRKYG QKQVKGSENP  240
RSYYKCTFPN CPTKKKVERS LDGQITQIVY KGSHNHPKPQ STRRSSSNAI QGSSYVISDQ  300
SVPTLSNPKV ESITLQEDSS TSMGEDEFEQ NSPISNSGGA EDENEPEAKR WKGENANDQA  360
YVSSGSRIVK EPRIVVQTTS EIDILDDGYR WRKYGQKVVK GNPNPRSYYK CTSVGCPVRK  420
HVERASHDTK AVITTYEGKH NHDVPAARGS GNYSNASRPA ADNSSNNVSM AVRPLALPNH  480
SNLRYLNSLQ NTRQPSTTES QPPFTLKMLQ SEGSYGFSEF *
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1wj2_A8e-38221446878Probable WRKY transcription factor 4
2lex_A8e-38221446878Probable WRKY transcription factor 4
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Mdo.158840.0fruit| leaf| stem
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: In dry seeds, expressed in aleurone cells and embryos. Levels drop rapidly but transiently in the embryos of imbibed seeds. {ECO:0000269|PubMed:19199048}.
UniprotTISSUE SPECIFICITY: Expressed in aleurone cells (PubMed:15618416). Mostly expressed in aleurone layers and leaves, and, to a lower extent, in roots, panicles and embryos (PubMed:26025535). {ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:26025535}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription activator (PubMed:26025535). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:19199048, PubMed:26025535). Negative regulator of both gibberellic acid (GA) and abscisic acid (ABA) signaling in aleurone cells, probably by interfering with GAM1, via the specific repression of GA- and ABA-induced promoters (PubMed:15618416, PubMed:19199048, PubMed:26025535). {ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:19199048, ECO:0000269|PubMed:26025535}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00299DAPTransfer from AT2G38470Download
Motif logo
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced by abscisic acid (ABA) in aleurone cells, embryos, roots and leaves (PubMed:15618416, PubMed:19199048). Slightly down-regulated by gibberellic acid (GA) (PubMed:15618416). Accumulates in response to jasmonic acid (MeJA) (By similarity). {ECO:0000250|UniProtKB:Q6B6R4, ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:19199048}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001281056.10.0probable WRKY transcription factor 33
SwissprotQ6IEQ71e-117WRK24_ORYSJ; WRKY transcription factor WRKY24
TrEMBLD9ZJG30.0D9ZJG3_MALDO; WRKY domain class transcription factor
STRINGXP_008370211.10.0(Malus domestica)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
FabidsOGEF107734114
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT2G38470.11e-118WRKY DNA-binding protein 33
Publications ? help Back to Top
  1. Kikuchi S, et al.
    Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice.
    Science, 2003. 301(5631): p. 376-9
    [PMID:12869764]
  2. Zhang ZL, et al.
    A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells.
    Plant Mol. Biol., 2009. 70(1-2): p. 139-51
    [PMID:19199048]
  3. Basu S,Roychoudhury A
    Expression profiling of abiotic stress-inducible genes in response to multiple stresses in rice (Oryza sativa L.) varieties with contrasting level of stress tolerance.
    Biomed Res Int, 2014. 2014: p. 706890
    [PMID:25110688]
  4. Zhang L, et al.
    Three WRKY transcription factors additively repress abscisic acid and gibberellin signaling in aleurone cells.
    Plant Sci., 2015. 236: p. 214-22
    [PMID:26025535]