PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

MDP0000301666

Organism

Malus domestica

Taxonomic ID

3750

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Maloideae; Maleae; Malus

Family

WRKY

Protein Properties

Length: 629aa MW: 68044.8 Da PI: 7.3585

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
MDP0000301666	genome	GDR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	96.8	1.5e-30	371	429	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 Dg++WrKYGqK+ kg+++pr+YYrCt+a gCpv+k+v+r+aed +++++tYeg+Hnh+ MDP0000301666 371 TDGCQWRKYGQKMAKGNPCPRAYYRCTMAvGCPVRKQVQRCAEDRTILITTYEGNHNHP 429 7*********************************************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	2.9E-33	355	431	IPR003657	WRKY domain
SuperFamily	SSF118290	1.57E-27	363	431	IPR003657	WRKY domain
PROSITE profile	PS50811	29.516	365	431	IPR003657	WRKY domain
SMART	SM00774	1.5E-36	370	430	IPR003657	WRKY domain
Pfam	PF03106	2.8E-26	372	429	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 629 aa Download sequence Send to blast
MDKGWGLTLD SDSFGYLLNK PPTAAVKLDH HNNKRSSFFG GERMFQGMEF PVKLGRREEQ 60 DASQPSNDNN RMVVDEVDFF SDRKNKLNTN STDDHHQDSK SNGAVSVKKE NWTGLDVNTG 120 LQLVTANTGS DQSMVDDGIS SDHGVNKRAK NHEIKVEFSI FRLMVYSVVG VDYDXQLAQL 180 QVEVQRMNAE NQRLKEMLGQ VTNNYSALQM HLAAVVQRQN HTTAAPADXR SQLDHDQNAE 240 AKSDQEKKQG LVPRQFLNLG PRATAETTDD QVSNSSSEAR TGSASPPQNI NEVAKNDQIG 300 PLDPPNSNNF RDGKRVGGDE SPESQSQGWV PNKVPKLNNS AAPKPIDQST EATMRKARVS 360 VRARSEAPMI TDGCQWRKYG QKMAKGNPCP RAYYRCTMAV GCPVRKQVQR CAEDRTILIT 420 TYEGNHNHPL PPAAMAMAST TTAAASMLLS GSMASADGMN MNPNNLLARA ILPCSSSVAT 480 ISASAPFPTV TLDLTTSPLQ FQRPNTPFQI PNFPGQPQSF GAASQQLPQG FGQALYNQSK 540 FSGLQLSQDL MGSTSSQQQQ MQQTQSSSFA DTVSAITADP SFTAALAAAI TSIIGGGAHP 600 SNNNNSASTT SNNSTGANSN KNISSFPGN

Sequence ? help Back to Top

Protein Sequence Length: 629 aa Download sequence Send to blast

MDKGWGLTLD SDSFGYLLNK PPTAAVKLDH HNNKRSSFFG GERMFQGMEF PVKLGRREEQ  60
DASQPSNDNN RMVVDEVDFF SDRKNKLNTN STDDHHQDSK SNGAVSVKKE NWTGLDVNTG  120
LQLVTANTGS DQSMVDDGIS SDHGVNKRAK NHEIKVEFSI FRLMVYSVVG VDYDXQLAQL  180
QVEVQRMNAE NQRLKEMLGQ VTNNYSALQM HLAAVVQRQN HTTAAPADXR SQLDHDQNAE  240
AKSDQEKKQG LVPRQFLNLG PRATAETTDD QVSNSSSEAR TGSASPPQNI NEVAKNDQIG  300
PLDPPNSNNF RDGKRVGGDE SPESQSQGWV PNKVPKLNNS AAPKPIDQST EATMRKARVS  360
VRARSEAPMI TDGCQWRKYG QKMAKGNPCP RAYYRCTMAV GCPVRKQVQR CAEDRTILIT  420
TYEGNHNHPL PPAAMAMAST TTAAASMLLS GSMASADGMN MNPNNLLARA ILPCSSSVAT  480
ISASAPFPTV TLDLTTSPLQ FQRPNTPFQI PNFPGQPQSF GAASQQLPQG FGQALYNQSK  540
FSGLQLSQDL MGSTSSQQQQ MQQTQSSSFA DTVSAITADP SFTAALAAAI TSIIGGGAHP  600
SNNNNSASTT SNNSTGANSN KNISSFPGN

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	3e-22	357	433	1	76	WRKY transcription factor 1
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Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Mdo.11201	0.0	bud\| cell culture\| fruit\| leaf\| root\| stem

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Roots, leaves, shoots, flowers and siliques.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the control of processes related to senescence and pathogen defense (PubMed:12000796). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:12000796). Activates the transcription of the SIRK gene and represses its own expression and that of the WRKY42 genes (PubMed:12000796). Modulates phosphate homeostasis and Pi translocation by regulating PHO1 expression (PubMed:25733771). {ECO:0000269\|PubMed:12000796, ECO:0000269\|PubMed:25733771}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid, ethylene, jasmonic acid, pathogens, wounding and strongly during leaf senescence. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11722756}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	JX112905	0.0	JX112905.1 Malus hupehensis WRKY transcription factor 42 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_008364089.2	0.0	probable WRKY transcription factor 31
Swissprot	Q9C519	1e-155	WRKY6_ARATH; WRKY transcription factor 6
TrEMBL	A0A498K0F0	0.0	A0A498K0F0_MALDO; Uncharacterized protein
STRING	XP_008364089.1	0.0	(Malus domestica)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF866	34	121

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G62300.1	1e-133	WRKY family protein

Link Out ? help Back to Top
Phytozome	MDP0000301666

Publications ? help Back to Top
Zhou F, et al. High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression. Plant J., 2004. 39(6): p. 920-32 [PMID:15341634] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P An extracellular subtilase switch for immune priming in Arabidopsis. PLoS Pathog., 2013. 9(6): p. e1003445 [PMID:23818851] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Singh V,Roy S,Singh D,Nandi AK Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. J. Biosci., 2014. 39(1): p. 119-26 [PMID:24499796] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Chai J,Liu J,Zhou J,Xing D Mitogen-activated protein kinase 6 regulates NPR1 gene expression and activation during leaf senescence induced by salicylic acid. J. Exp. Bot., 2014. 65(22): p. 6513-28 [PMID:25210078] Vermeirssen V,De Clercq I,Van Parys T,Van Breusegem F,Van de Peer Y Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress. Plant Cell, 2014. 26(12): p. 4656-79 [PMID:25549671] Xiao D, et al. SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE Directly Interacts with the Cytoplasmic Domain of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE and Negatively Regulates Leaf Senescence in Arabidopsis. Plant Physiol., 2015. 169(2): p. 1275-91 [PMID:26304848] Bakshi M, et al. WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis. BMC Plant Biol., 2015. 15: p. 305 [PMID:26718529] Huang Y,Feng CZ,Ye Q,Wu WH,Chen YF Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development. PLoS Genet., 2016. 12(2): p. e1005833 [PMID:26829043] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P Correction: An Extracellular Subtilase Switch for Immune Priming in Arabidopsis. PLoS Pathog., 2016. 12(11): p. e1006003 [PMID:27806116] Li H, et al. BZR1 Positively Regulates Freezing Tolerance via CBF-Dependent and CBF-Independent Pathways in Arabidopsis. Mol Plant, 2017. 10(4): p. 545-559 [PMID:28089951] Stetter MG,Benz M,Ludewig U Increased root hair density by loss of WRKY6 in Arabidopsis thaliana. PeerJ, 2017. 5: p. e2891 [PMID:28149680] Zhou X, et al. StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato. J. Exp. Bot., 2017. 68(5): p. 1265-1281 [PMID:28338870] Bakshi M, et al. Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation. Front Microbiol, 2017. 8: p. 1262 [PMID:28747898] Zhang Y, et al. DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6. Plant Cell Rep., 2018. 37(7): p. 981-992 [PMID:29574486]