PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Lj2g3v2314850.1

Organism

Lotus japonicus

Taxonomic ID

34305

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Loteae; Lotus

Family

WRKY

Protein Properties

Length: 1232aa MW: 140831 Da PI: 6.3945

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Lj2g3v2314850.1	genome	Kazusa	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	82.4	4.5e-26	594	650	2	58
--SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnh 58 +Dg++WrKYGqK+++ s++pr+YYrCt ++C +kk+v+r ++ p++ e+tY++eH++ Lj2g3v2314850.1 594 EDGFTWRKYGQKVIHASKYPRNYYRCTRQKCLAKKRVQRLDDTPEIYEVTYRNEHTC 650 7******************************************************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF74788	6.8E-69	231	553	IPR016159	Cullin repeat-like-containing domain
Pfam	PF03081	2.6E-65	318	554	IPR004140	Exocyst complex protein Exo70
Gene3D	G3DSA:2.20.25.80	3.1E-25	592	651	IPR003657	WRKY domain
SuperFamily	SSF118290	7.45E-24	592	651	IPR003657	WRKY domain
SMART	SM00774	5.0E-33	593	652	IPR003657	WRKY domain
PROSITE profile	PS50811	20.392	594	648	IPR003657	WRKY domain
Pfam	PF03106	1.1E-22	594	650	IPR003657	WRKY domain
SuperFamily	SSF74788	2.04E-74	808	1232	IPR016159	Cullin repeat-like-containing domain
Pfam	PF03081	1.0E-72	895	1232	IPR004140	Exocyst complex protein Exo70

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0006887	Biological Process	exocytosis
GO:0000145	Cellular Component	exocyst
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 1232 aa Download sequence Send to blast
MTRWLIQILS WLMQPNVWRF VSFASAVVGL LCNALSSSFN HLFGDWTFFK ISLYSVFSVI 60 MSLMMLFAKK WRHSRSLQFK AHLAFLVLTI TSLYSFISDK VMNGKPDVYS LISCAAFAIM 120 SLSISRQIQC GFEVDLLYFF LGCLMVQLMK TKLVLVIVGA GFSYSLIVLR SSFSSIDGLP 180 KIDHIPAPQE EPSIDILVDS QHLARTDIAS VTQQLSTHLK DLQQHDSNLR KLLLERVKEY 240 LEHNSELVAT DHNFIIDALP SETLNKFHKT AKLMVEAGFE KVFFAVYSSW REEWLKNLLG 300 DTHKMAWQDL KDETGFGRWV KASNVALKIL FPNERQLCDR ILLGFSSAAD FSFTEVCRGS 360 VIQLLNFADV VAIGKRSLQR LSRILEVFET LTELIPEYES LFCDQYSLSL RNKAITIWKR 420 LGEAIRGILN VLESLVWRDP AKEAAPGGAV HPFTRYVMYY LQETCRSWQT LEKVFENYSL 480 SAQMHWIIEL LESNLEAKST FYKDPAVGFV FLMNNGDYIV QKAKDSELGT LLGDDWIQKH 540 TTKVRQYLLQ FRRTMTKQSA ATSVQDLPGR TKNAPERTVR FPAPQVGDLG MPPEDGFTWR 600 KYGQKVIHAS KYPRNYYRCT RQKCLAKKRV QRLDDTPEIY EVTYRNEHTC YMSLAEPSLL 660 LPSQQVDISK YMTQPTMSLQ FSPSSSSVSG QSFKNLVQQL SGSGSTGGGV GPSTSEYSTD 720 YPIADMVDAM FTSSCSNVSS VASFVPFAED KWEPDGAPEN EHSEVIEVDS PQLVSTDIAG 780 MMQQLSNCMK GLQQHDCKLT NILLMPKEHV DDKSMLLRTD INFVIDALPS ERINSLHKTV 840 KLMVASGFEE ECSKVYSSWR RRWLEECLIN KLLELQENEI ENEHEKKTCL DKRVERWIEA 900 SQVALLILFR EWRLCNRIFS GFSSAADSCF TQVCQGPMIQ MLNFAAAVAD GSPSAYRLFK 960 ILDMFMTLNA LIPEFQSLLF PNPLVNEAIA VQNRLGEASR DLFMEFEILV FDIPEVKLLA 1020 PPNGGHHPMM NNIVAYLNSA YRSQQILEQI LQQYPKVATG AETSSFMTQM KRTLKLLEKT 1080 LVEKSENYKD PALPYFFMMN NWRLIEVALG TMYGHDLFQN NRAKVQKNLE QYRGSSWNKM 1140 LDILKVESNE SEAPNVAADL MKDKLNLFNM HFEEICRVQS TWSVIDEQLR EEIRISVNDI 1200 LLPAYGNFIG RFQDVLGKHA YEYIEYGLFD IE

Sequence ? help Back to Top

Protein Sequence Length: 1232 aa Download sequence Send to blast

MTRWLIQILS WLMQPNVWRF VSFASAVVGL LCNALSSSFN HLFGDWTFFK ISLYSVFSVI  60
MSLMMLFAKK WRHSRSLQFK AHLAFLVLTI TSLYSFISDK VMNGKPDVYS LISCAAFAIM  120
SLSISRQIQC GFEVDLLYFF LGCLMVQLMK TKLVLVIVGA GFSYSLIVLR SSFSSIDGLP  180
KIDHIPAPQE EPSIDILVDS QHLARTDIAS VTQQLSTHLK DLQQHDSNLR KLLLERVKEY  240
LEHNSELVAT DHNFIIDALP SETLNKFHKT AKLMVEAGFE KVFFAVYSSW REEWLKNLLG  300
DTHKMAWQDL KDETGFGRWV KASNVALKIL FPNERQLCDR ILLGFSSAAD FSFTEVCRGS  360
VIQLLNFADV VAIGKRSLQR LSRILEVFET LTELIPEYES LFCDQYSLSL RNKAITIWKR  420
LGEAIRGILN VLESLVWRDP AKEAAPGGAV HPFTRYVMYY LQETCRSWQT LEKVFENYSL  480
SAQMHWIIEL LESNLEAKST FYKDPAVGFV FLMNNGDYIV QKAKDSELGT LLGDDWIQKH  540
TTKVRQYLLQ FRRTMTKQSA ATSVQDLPGR TKNAPERTVR FPAPQVGDLG MPPEDGFTWR  600
KYGQKVIHAS KYPRNYYRCT RQKCLAKKRV QRLDDTPEIY EVTYRNEHTC YMSLAEPSLL  660
LPSQQVDISK YMTQPTMSLQ FSPSSSSVSG QSFKNLVQQL SGSGSTGGGV GPSTSEYSTD  720
YPIADMVDAM FTSSCSNVSS VASFVPFAED KWEPDGAPEN EHSEVIEVDS PQLVSTDIAG  780
MMQQLSNCMK GLQQHDCKLT NILLMPKEHV DDKSMLLRTD INFVIDALPS ERINSLHKTV  840
KLMVASGFEE ECSKVYSSWR RRWLEECLIN KLLELQENEI ENEHEKKTCL DKRVERWIEA  900
SQVALLILFR EWRLCNRIFS GFSSAADSCF TQVCQGPMIQ MLNFAAAVAD GSPSAYRLFK  960
ILDMFMTLNA LIPEFQSLLF PNPLVNEAIA VQNRLGEASR DLFMEFEILV FDIPEVKLLA  1020
PPNGGHHPMM NNIVAYLNSA YRSQQILEQI LQQYPKVATG AETSSFMTQM KRTLKLLEKT  1080
LVEKSENYKD PALPYFFMMN NWRLIEVALG TMYGHDLFQN NRAKVQKNLE QYRGSSWNKM  1140
LDILKVESNE SEAPNVAADL MKDKLNLFNM HFEEICRVQS TWSVIDEQLR EEIRISVNDI  1200
LLPAYGNFIG RFQDVLGKHA YEYIEYGLFD IE

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4rl5_A	2e-52	259	1232	129	548	Exocyst complex component EXO70A1
4rl5_B	2e-52	259	1232	129	548	Exocyst complex component EXO70A1
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Lja.3879	1e-167	floral bud\| pod

Functional Description ? help Back to Top
Source	Description
UniProt	Component of an exocyst subcomplex specifically involved in autophagy-related, Golgi-independent membrane traffic to the vacuole. Regulates autophagosome formation and autophagy-related Golgi-independent import into the vacuole. Positive regulator of both abscisic acid (ABA)-promoted and mannitol (drought)-promoted stomatal closure (PubMed:27956469). {ECO:0000269\|PubMed:23944713, ECO:0000269\|PubMed:27956469}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Lj2g3v2314850.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_004490669.1	0.0	uncharacterized protein LOC101500706 isoform X2
Swissprot	Q9FGH9	1e-115	E70B1_ARATH; Exocyst complex component EXO70B1
TrEMBL	A0A1S2XKF6	0.0	A0A1S2XKF6_CICAR; Exocyst subunit Exo70 family protein
STRING	XP_004490668.1	0.0	(Cicer arietinum)
STRING	AES98854	0.0	(Medicago truncatula)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF1741	4	5

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G40740.1	5e-25	WRKY DNA-binding protein 55

Publications ? help Back to Top
Synek L, et al. AtEXO70A1, a member of a family of putative exocyst subunits specifically expanded in land plants, is important for polar growth and plant development. Plant J., 2006. 48(1): p. 54-72 [PMID:16942608] Li S, et al. Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis. Plant Physiol., 2010. 154(4): p. 1819-30 [PMID:20943851] Wang J, et al. EXPO, an exocyst-positive organelle distinct from multivesicular endosomes and autophagosomes, mediates cytosol to cell wall exocytosis in Arabidopsis and tobacco cells. Plant Cell, 2010. 22(12): p. 4009-30 [PMID:21193573] Kulich I, et al. Arabidopsis exocyst subcomplex containing subunit EXO70B1 is involved in autophagy-related transport to the vacuole. Traffic, 2013. 14(11): p. 1155-65 [PMID:23944713] Stegmann M, et al. The exocyst subunit Exo70B1 is involved in the immune response of Arabidopsis thaliana to different pathogens and cell death. Plant Signal Behav, 2013. 8(12): p. e27421 [PMID:24389869] Tzfadia O,Galili G The Arabidopsis exocyst subcomplex subunits involved in a golgi-independent transport into the vacuole possess consensus autophagy-associated atg8 interacting motifs. Plant Signal Behav, 2013. 8(10): p. doi: 10.4161/psb.26732 [PMID:24494242] Zhao T, et al. A truncated NLR protein, TIR-NBS2, is required for activated defense responses in the exo70B1 mutant. PLoS Genet., 2015. 11(1): p. e1004945 [PMID:25617755] Hong D,Jeon BW,Kim SY,Hwang JU,Lee Y The ROP2-RIC7 pathway negatively regulates light-induced stomatal opening by inhibiting exocyst subunit Exo70B1 in Arabidopsis. New Phytol., 2016. 209(2): p. 624-35 [PMID:26451971] Seo DH,Ahn MY,Park KY,Kim EY,Kim WT The N-Terminal UND Motif of the Arabidopsis U-Box E3 Ligase PUB18 Is Critical for the Negative Regulation of ABA-Mediated Stomatal Movement and Determines Its Ubiquitination Specificity for Exocyst Subunit Exo70B1. Plant Cell, 2016. 28(12): p. 2952-2973 [PMID:27956469] Sabol P,Kulich I,Žárský V RIN4 recruits the exocyst subunit EXO70B1 to the plasma membrane. J. Exp. Bot., 2017. 68(12): p. 3253-3265 [PMID:28338727] Liu N, et al. CALCIUM-DEPENDENT PROTEIN KINASE5 Associates with the Truncated NLR Protein TIR-NBS2 to Contribute to exo70B1-Mediated Immunity. Plant Cell, 2017. 29(4): p. 746-759 [PMID:28351987]