PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

HL.SW.v1.0.G014106.1

Organism

Humulus lupulus

Taxonomic ID

3486

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Cannabaceae; Humulus

Family

bHLH

Protein Properties

Length: 616aa MW: 67559.5 Da PI: 4.7288

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
HL.SW.v1.0.G014106.1	genome	HOPBASE	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	37.3	5e-12	225	276	1	55
CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +r++h ++Er+RR+++N+ + Lr+l+P + + ++ + a+i A+e++++L+ HL.SW.v1.0.G014106.1 225 QRMTHIAVERNRRKQMNEHLRVLRSLMPGS---YVQRGDQASIIGGAIEFVRELE 276 79**************************...9***************9995 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:4.10.280.10	2.5E-13	223	279	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	6.27E-13	223	280	No hit	No description
PROSITE profile	PS50888	15.428	224	275	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	3.53E-16	224	290	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	2.3E-9	225	276	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	3.4E-9	230	281	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF55021	8.59E-5	542	599	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0010377	Biological Process	guard cell fate commitment
GO:0045597	Biological Process	positive regulation of cell differentiation
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0051782	Biological Process	negative regulation of cell division
GO:0005634	Cellular Component	nucleus
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 616 aa Download sequence Send to blast
MAPSFSSLDY TIDNNHQFMK SHIDETSSEA GADQNVDDGR LMVNYLLNMN NVNNNSNSSS 60 HHNQQLSFAD VMQFADFGPK LGLNQTNIPA EDDDDDEEED HRHHTGQPGS NMVDPVYFLK 120 FPVLNDKNSN MMVQQDQSQS IINDVVRSSQ AAAAAGNDVV GLKDQLDHDD DDDDHDDDGS 180 MHLQFLGEDL QSKNPSSEPN NNNNNNKNKR KRPRTVKTSE EVESQRMTHI AVERNRRKQM 240 NEHLRVLRSL MPGSYVQRGD QASIIGGAIE FVRELEQLLQ CLESQKRRRL VGDQAPAAAG 300 RQQLGADQGG GTGGGPGAVV PGTPGFFPPM PLPNDQMMMN NKFVADFETG GGLREEMAES 360 KSCLADVEVK LLGFDAMIKI LSRRRPGQLI KAIAALEDLQ FNILHTNITT IEQTVLYSFN 420 VKGDQASIIG GAIEFVRELE QLLQCLESQK RRRLVGDQAS AAAGRQQLGA DQGGGTGGGP 480 GAVVPGTPGF FPPMPLPNDQ MMMMNNKFVA DFETGGGLRE EMAESKSCLA DVEVKLLGFD 540 AMIKILSRRR PGQLIKAIAA LEDLQFNILH TNITTIEQTV LYSFNVKVTS DSRFTAEDIA 600 GSVQQIFSFI HANSL*

Sequence ? help Back to Top

Protein Sequence Length: 616 aa Download sequence Send to blast

MAPSFSSLDY TIDNNHQFMK SHIDETSSEA GADQNVDDGR LMVNYLLNMN NVNNNSNSSS  60
HHNQQLSFAD VMQFADFGPK LGLNQTNIPA EDDDDDEEED HRHHTGQPGS NMVDPVYFLK  120
FPVLNDKNSN MMVQQDQSQS IINDVVRSSQ AAAAAGNDVV GLKDQLDHDD DDDDHDDDGS  180
MHLQFLGEDL QSKNPSSEPN NNNNNNKNKR KRPRTVKTSE EVESQRMTHI AVERNRRKQM  240
NEHLRVLRSL MPGSYVQRGD QASIIGGAIE FVRELEQLLQ CLESQKRRRL VGDQAPAAAG  300
RQQLGADQGG GTGGGPGAVV PGTPGFFPPM PLPNDQMMMN NKFVADFETG GGLREEMAES  360
KSCLADVEVK LLGFDAMIKI LSRRRPGQLI KAIAALEDLQ FNILHTNITT IEQTVLYSFN  420
VKGDQASIIG GAIEFVRELE QLLQCLESQK RRRLVGDQAS AAAGRQQLGA DQGGGTGGGP  480
GAVVPGTPGF FPPMPLPNDQ MMMMNNKFVA DFETGGGLRE EMAESKSCLA DVEVKLLGFD  540
AMIKILSRRR PGQLIKAIAA LEDLQFNILH TNITTIEQTV LYSFNVKVTS DSRFTAEDIA  600
GSVQQIFSFI HANSL*

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	225	236	RMTHIAVERNRR
2	449	453	KRRRL

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator (PubMed:17088607, PubMed:17183265, PubMed:17183267). Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity (PubMed:24571519). Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata (PubMed:17088607, PubMed:17183265, PubMed:17183267). Prevents histone H3K27me3 marks and derepresses stem cell gene expression (PubMed:24654956). {ECO:0000269\|PubMed:17088607, ECO:0000269\|PubMed:17183265, ECO:0000269\|PubMed:17183267, ECO:0000269\|PubMed:24571519, ECO:0000269\|PubMed:24654956}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Inhibited by low relative humidity (LRH) via epigenetic CG methylation, thus leading to a reduced stomatal index. {ECO:0000269\|PubMed:22442411}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_024028877.1	1e-143	transcription factor FAMA isoform X1
Swissprot	Q56YJ8	1e-110	FAMA_ARATH; Transcription factor FAMA
TrEMBL	A0A2P5DI01	1e-147	A0A2P5DI01_TREOI; Basic helix-loop-helix transcription factor
STRING	XP_010107771.1	1e-142	(Morus notabilis)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF10489	33	40

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G24140.1	3e-92	bHLH family protein

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Matos JL, et al. Irreversible fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RETINOBLASTOMA-RELATED module. Elife, 2015. [PMID:25303364] Chen L, et al. NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development, 2016. 143(9): p. 1600-11 [PMID:26989174] Lee JH,Jung JH,Park CM Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis. Plant Cell, 2017. 29(11): p. 2817-2830 [PMID:29070509] Han X, et al. Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons. Plant Physiol., 2018. 176(4): p. 2871-2885 [PMID:29496884] Li X,Yang R,Chen H The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA. PLoS ONE, 2018. 13(3): p. e0193458 [PMID:29513733]