PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Glyma.03G115400.1.p
Common NameGLYMA_03G115400, LOC100805384
Organism
Glycine max
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Phaseoleae; Glycine; Soja
Family MYB
Protein Properties Length: 312aa    MW: 35625.1 Da    PI: 5.0322
Description MYB family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Glyma.03G115400.1.pgenomeJGIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1Myb_DNA-binding41.14e-131358146
                         TSSS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHHHH CS
      Myb_DNA-binding  1 rgrWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrwqk 46
                         +g W +eEde l++ vk++G+++W++I ++  + Rt+k+c++rw +
  Glyma.03G115400.1.p 13 KGQWEAEEDEVLLNHVKKYGPRDWSSIRSKGLLQRTGKSCRLRWVN 58
                         799************************8887799**********87 PP
2Myb_DNA-binding43.19.9e-1469108344
                          SS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHH CS
      Myb_DNA-binding   3 rWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrw 44 
                          +++ eE+   +++ +q+G++ W++Ia++++ gRt++++k++w
  Glyma.03G115400.1.p  69 KFSLEEERVVIELQAQFGNR-WAKIASYLP-GRTDNDVKNFW 108
                          799*****************.*********.*********** PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:1.10.10.602.2E-20661IPR009057Homeodomain-like
PROSITE profilePS5129414.107860IPR017930Myb domain
SMARTSM007171.6E-91262IPR001005SANT/Myb domain
SuperFamilySSF466891.45E-2712108IPR009057Homeodomain-like
PfamPF139215.2E-131675No hitNo description
CDDcd001671.42E-101660No hitNo description
PROSITE profilePS5129419.46361116IPR017930Myb domain
Gene3DG3DSA:1.10.10.605.7E-1862114IPR009057Homeodomain-like
SMARTSM007176.6E-1366114IPR001005SANT/Myb domain
CDDcd001671.26E-1069108No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0003677Molecular FunctionDNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 312 aa     Download sequence    Send to blast
MHGKKEQEEY IRKGQWEAEE DEVLLNHVKK YGPRDWSSIR SKGLLQRTGK SCRLRWVNKL  60
RPNLKNGCKF SLEEERVVIE LQAQFGNRWA KIASYLPGRT DNDVKNFWSS RQKRLARILQ  120
TSATTFRSQK NKTKVPSFLD VPTLEAPKFS SSSEGEPSSK PQTCTLPYID NSEVIQMVSL  180
PDLIKSELPS SDTIHVEQEF TLFENYKSTE QIAFPQIPEL QNGLTFSMEN QDLVPTIEEP  240
CFIEDLCPFD VSEFGIGPQH PFGFPFFEPP GSCRFGTRDA IDNFKISDSF FDDLPVDMFD  300
HMDPPASPSN L*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1h8a_C7e-271111425126MYB TRANSFORMING PROTEIN
Search in ModeBase
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Strict spatial and temporal transcriptional activation leading to a specific expression in the male germline cells; this specific expression pattern is dependent of the regulatory region of DUO1 (ROD1) 5'-YAACYGY-3' in its promoter (PubMed:15694308, PubMed:27624837). First observed in the germ cell during or soon after engulfment by the vegetative cell, just after asymmetric division. In maturating pollen, accumulates in germ cells before mitosis and remains high in mature sperm cells. Expression persists during pollen development (PubMed:19300502). {ECO:0000269|PubMed:15694308, ECO:0000269|PubMed:19300502, ECO:0000269|PubMed:27624837}.
UniprotTISSUE SPECIFICITY: Confined to inflorescences, especially in stamens and pollen. {ECO:0000269|PubMed:15694308}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription activator that acts as a positive regulator of male germline development by promoting both gametic cell specification and cell cycle progression (PubMed:15694308, PubMed:15618418, PubMed:19300502, PubMed:21285328). Binds to canonical MYB sites 5'-AACCGTC-3', 5'-AAACCGC-3' and 5'-AACCGT-3' in promoters to trigger the expression of male germline-specific or enriched genes (e.g. MGH3, GEX2 and GCS1), including those required for fertilization (PubMed:21285328, PubMed:19300502). Required for sperm cell specification leading to pollen maturation by activating a germline-specific regulon (PubMed:21285328, PubMed:15694308, PubMed:15618418, PubMed:19300502). Involved in pollen mitosis entry at G2-M transition via the regulation of CYCB1-1, DAZ1 and DAZ2 expression (PubMed:15618418, PubMed:19300502, PubMed:24876252). {ECO:0000269|PubMed:15618418, ECO:0000269|PubMed:15694308, ECO:0000269|PubMed:19300502, ECO:0000269|PubMed:21285328, ECO:0000269|PubMed:24876252}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapGlyma.03G115400.1.p
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by the microRNA 159 (miR159); the production of miR159 is stimulated by the anaphase promoting complex/cyclosome (APC/C) (PubMed:21441434). Activated by ARID1 in male germline cells via specific histone acetylation regulation (e.g. H3K9Ac) (PubMed:25057814). {ECO:0000269|PubMed:21441434, ECO:0000269|PubMed:25057814}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAP0150421e-111AP015042.1 Vigna angularis var. angularis DNA, chromosome 9, almost complete sequence, cultivar: Shumari.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_003520442.10.0transcription factor DUO1
RefseqXP_028223798.10.0transcription factor DUO1-like
SwissprotA0A178VEK71e-80DUO1_ARATH; Transcription factor DUO1
TrEMBLA0A445LA980.0A0A445LA98_GLYSO; Transcription factor DUO1 (Fragment)
STRINGGLYMA03G26830.20.0(Glycine max)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
FabidsOGEF36933164
Representative plantOGRP5171784
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G60460.14e-76MYB family protein
Publications ? help Back to Top
  1. Zheng B,He H,Zheng Y,Wu W,McCormick S
    An ARID domain-containing protein within nuclear bodies is required for sperm cell formation in Arabidopsis thaliana.
    PLoS Genet., 2014. 10(7): p. e1004421
    [PMID:25057814]
  2. Peters B, et al.
    A Conserved cis-Regulatory Module Determines Germline Fate through Activation of the Transcription Factor DUO1 Promoter.
    Plant Physiol., 2017. 173(1): p. 280-293
    [PMID:27624837]
  3. Zheng Z, et al.
    Target RNA Secondary Structure Is a Major Determinant of miR159 Efficacy.
    Plant Physiol., 2017. 174(3): p. 1764-1778
    [PMID:28515145]