PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Gh_D12G0491
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium
Family ARF
Protein Properties Length: 1354aa    MW: 150830 Da    PI: 6.2857
Description ARF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Gh_D12G0491genomeNAU-NBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1B371.88.6e-23128229199
                  EEEE-..-HHHHTT-EE--HHH.HTT.......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-EEEEEE-SSSEE. CS
           B3   1 ffkvltpsdvlksgrlvlpkkfaeeh.......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfvvFkldgrsefe 91 
                  f+k+lt sd++++g +++p++ ae+        +++++  ++l+ +d++  +W++++i+r++++r++lt+GW+ Fv+a++L +gD+v+F   ++++ +
  Gh_D12G0491 128 FCKTLTASDTSTHGGFSVPRRAAEKVfppldfsQQPPA--QELIARDLHDIEWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFI--WNENNQ 221
                  99*****************************8544444..48************************************************..788888 PP

                  .EEEEE-S CS
           B3  92 lvvkvfrk 99 
                  l+++++r+
  Gh_D12G0491 222 LLLGIRRA 229
                  9****997 PP

2Auxin_resp108.85.8e-36254338183
   Auxin_resp   1 aahaastksvFevvYnPr.astseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 
                  aahaa+t+s F+v+YnPr as+seFv++++k+ ka+  ++vsvGmRf+m fete+ss rr++Gtv+g+sdld+vrWpnS+Wrs+k
  Gh_D12G0491 254 AAHAAATNSCFTVFYNPRwASPSEFVIPLSKYIKAVFhTRVSVGMRFRMLFETEESSVRRYMGTVTGISDLDQVRWPNSHWRSVK 338
                  79***************85899************986599******************************************985 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF1019364.32E-49112257IPR015300DNA-binding pseudobarrel domain
Gene3DG3DSA:2.40.330.102.8E-41121243IPR015300DNA-binding pseudobarrel domain
CDDcd100176.47E-21127228No hitNo description
SMARTSM010198.0E-24128230IPR003340B3 DNA binding domain
PfamPF023624.7E-21128229IPR003340B3 DNA binding domain
PROSITE profilePS5086312.407128230IPR003340B3 DNA binding domain
PfamPF065072.5E-31254338IPR010525Auxin response factor
SuperFamilySSF542773.73E-7691772No hitNo description
PfamPF023091.1E-7692782IPR033389AUX/IAA domain
PROSITE profilePS5174524.019698782IPR000270PB1 domain
Gene3DG3DSA:2.60.200.204.4E-17788920IPR000253Forkhead-associated (FHA) domain
CDDcd000603.13E-13824918No hitNo description
SuperFamilySSF498798.03E-17824927IPR008984SMAD/FHA domain
SMARTSM002401.4E-4832885IPR000253Forkhead-associated (FHA) domain
PROSITE profilePS5000611.095833885IPR000253Forkhead-associated (FHA) domain
PfamPF004988.5E-12833909IPR000253Forkhead-associated (FHA) domain
CDDcd000271.30E-4922989No hitNo description
Gene3DG3DSA:3.40.50.101905.4E-6931989IPR001357BRCT domain
SuperFamilySSF521131.22E-5933991IPR001357BRCT domain
PROSITE profilePS501729.139938988IPR001357BRCT domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009725Biological Processresponse to hormone
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0005515Molecular Functionprotein binding
Sequence ? help Back to Top
Protein Sequence    Length: 1354 aa     Download sequence    Send to blast
MKLSASGLGQ QAAHEGENKC LNSELWHACA GPLVCLPTVG SRVVYFPQGH SEQVAATTNK  60
EVDAHIPNYP NLPPQLICQL HNVTMHADVE TDEVYAQMTL QPLTPEEQKD TFLPMELGIP  120
SKQPTNYFCK TLTASDTSTH GGFSVPRRAA EKVFPPLDFS QQPPAQELIA RDLHDIEWKF  180
RHIFRGQPKR HLLTTGWSVF VSAKRLVAGD SVLFIWNENN QLLLGIRRAT RPQTVMPSSV  240
LSSDSMHIGL LAAAAHAAAT NSCFTVFYNP RWASPSEFVI PLSKYIKAVF HTRVSVGMRF  300
RMLFETEESS VRRYMGTVTG ISDLDQVRWP NSHWRSVKVG WDESTAGERQ PRVSLWEIEP  360
LTTFPMYPSL FPLRLKRPWH PGSSSLHDNR DDVSSGLMWL RGGSGESFST FPWMQQRVDP  420
NFPVNDHNLQ YQAMLANGLQ NLGSGDPLRQ QLQQSLPYVQ QPGNLSLLLQ QQQQRGVSQS  480
VPHNIVQVQS QILSESLPSV LGREQVSNHP EEQVQQQHNM IQSDQLLQRQ PANLPSSFLK  540
TDFINSGKFS GSVPPVQNML GSLCPESSAN LLNFSRSGQS MLAGQLTPRS WAPKYTHSEV  600
NAFGSSTSLP QVFPGKDAVI EPDIGNSGAQ NSALFGNNNS YGLLLPTMMP AIATSSCEAD  660
VPSIPLGDSS FQNPLYGCMQ DSSELQSRGQ VDPPTPTPTF VKVYKSGSVG RSLDISRFSS  720
YHELREELAQ MFGIEGKLED PLRSGWQLVF VDRENDILLL GDDPWEAFVN NVWYIKILSP  780
EDVQKMGEQR AEPFSPTTPG QRMNSTGTDT GTAEQEEEEE EEDKYYIFKN GTYSVGRKGC  840
DIIIHKDKGV SRIHAHIMVD EMTQSCQVRI RDLSKYGTFI NKNLSSNKKV HHFPNKQTFL  900
EDGDLLSFGT GNATFRFSYV PLVLYICCAE ASQMNHHLQD KVSSIGARLT HVYSEDCTHV  960
IVDQHVPLKR DMLDAIVAKK PLLRTSWLEL VAERSIQTKF PDCCSHVPTI MVDGVFVEVS  1020
DPKTRENCLG GYTFLLESTQ VYKFGDRLQS LLEVSGSRVN YVEEICSSSQ AFGCGENTCL  1080
TYVIPGRSVD KFDHLDNLGL SYRVDEMALI RALLSGKLDQ SILISSSVPV SSSCSTDETV  1140
VADSDEEIEM ATSVNVNATT CKEGAQNYVS RDGISMEAPS CVSKAEVPTC QDAIRLKDDR  1200
VTSKDDNCCL TARRDKADQV TSKDDNCCLT ARRDKADESE YGSSNIVYSQ VLIVRDVNIT  1260
SKNRFKEDNG VINYKRFRKA SIQSGNSFNN LVPFSKHPYK DYEFGSGDLA ESVKEEKKRK  1320
KMNAIADDMF NSEKGGRQRG LSGSLRRGLL HTRG
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4ldu_A1e-1532135951388Auxin response factor 5
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
113161320KKRKK
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Ghi.20380.0boll| ovule| stem
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Expressed in sepals at stages 11, 12 and 13 of flower development. Highly expressed in petals at stages 9-10, decreases at stage 11 and disappears after flower stage 12. In anthers, expressed at stage 11 in the tapetum, disappears early in stage 12 when the tapetum degrades and reappears throughout the anther late in stage 12 to persist at least until stage 13. In stamen filaments, expressed at stages 12 to 13, especially near the apical end of the filament. Expressed throughout the gynoecium at early stages up to stage 12, especially strongly in ovules. Expression in gynoecium decreases late in stage 12, but persists through stage 13, especially near the apical end including the style. Expressed in the mesocarp of the fruit and the carpel septum during fruit growth. {ECO:0000269|PubMed:16107481, ECO:0000269|PubMed:16829592}.
UniprotTISSUE SPECIFICITY: Expressed in the whole plant. {ECO:0000269|PubMed:10476078}.
Functional Description ? help Back to Top
Source Description
UniProtAuxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Regulates both stamen and gynoecium maturation. Promotes jasmonic acid production. Partially redundant with ARF6. Involved in fruit initiation. Acts as an inhibitor to stop further carpel development in the absence of fertilization and the generation of signals required to initiate fruit and seed development. {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:16107481, ECO:0000269|PubMed:16829592}.
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by miR167. {ECO:0000269|PubMed:17021043}.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_012436680.10.0PREDICTED: auxin response factor 8-like isoform X1
SwissprotQ9FGV10.0ARFH_ARATH; Auxin response factor 8
TrEMBLA0A0D2SVH70.0A0A0D2SVH7_GOSRA; Auxin response factor
STRINGGorai.008G054600.10.0(Gossypium raimondii)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM13442894
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G37020.10.0auxin response factor 8
Publications ? help Back to Top
  1. Liu N, et al.
    Down-regulation of AUXIN RESPONSE FACTORS 6 and 8 by microRNA 167 leads to floral development defects and female sterility in tomato.
    J. Exp. Bot., 2014. 65(9): p. 2507-20
    [PMID:24723401]
  2. Minato N, et al.
    The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways.
    Sci Rep, 2014. 4: p. 7399
    [PMID:25492247]
  3. Ortega-Amaro MA, et al.
    Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance.
    Front Plant Sci, 2014. 5: p. 782
    [PMID:25653657]
  4. Wang Y, et al.
    MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.
    Plant Physiol., 2015. 168(3): p. 984-99
    [PMID:25941314]
  5. Barik S, et al.
    Coevolution Pattern and Functional Conservation or Divergence of miR167s and their targets across Diverse Plant Species.
    Sci Rep, 2015. 5: p. 14611
    [PMID:26459056]
  6. Pashkovskiy PP,Kartashov AV,Zlobin IE,Pogosyan SI,Kuznetsov VV
    Blue light alters miR167 expression and microRNA-targeted auxin response factor genes in Arabidopsis thaliana plants.
    Plant Physiol. Biochem., 2016. 104: p. 146-54
    [PMID:27031426]
  7. Mlotshwa S,Pruss GJ,MacArthur JL,Reed JW,Vance V
    Developmental Defects Mediated by the P1/HC-Pro Potyviral Silencing Suppressor Are Not Due to Misregulation of AUXIN RESPONSE FACTOR 8.
    Plant Physiol., 2016. 172(3): p. 1853-1861
    [PMID:27688620]
  8. Wójcikowska B,Gaj MD
    Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis.
    Plant Cell Rep., 2017. 36(6): p. 843-858
    [PMID:28255787]
  9. Zhang GZ, et al.
    Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis.
    Plant Cell Rep., 2017. 36(12): p. 1995-2006
    [PMID:29027578]
  10. Zheng K, et al.
    Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1813
    [PMID:29114256]
  11. Liu Z, et al.
    ARF2-ARF4 and ARF5 are Essential for Female and Male Gametophyte Development in Arabidopsis.
    Plant Cell Physiol., 2018. 59(1): p. 179-189
    [PMID:29145642]
  12. Ghelli R, et al.
    A Newly Identified Flower-Specific Splice Variant of AUXIN RESPONSE FACTOR8 Regulates Stamen Elongation and Endothecium Lignification in Arabidopsis.
    Plant Cell, 2018. 30(3): p. 620-637
    [PMID:29514943]