PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Gh_D12G0259

Organism

Gossypium hirsutum

Taxonomic ID

3635

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium

Family

bHLH

Protein Properties

Length: 423aa MW: 46652.6 Da PI: 5.9214

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Gh_D12G0259	genome	NAU-NBI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	30.7	5.5e-10	227	274	4	55
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +h+ +Er RR++i +++ L++l+P + +k Ka +L + ++Y++sLq Gh_D12G0259 227 SHSLAERVRREKISERMKYLQDLVPGC----NKITGKAGMLDEIINYVQSLQ 274 8************************....999***************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47459	7.33E-17	221	291	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	1.98E-10	221	278	No hit	No description
Gene3D	G3DSA:4.10.280.10	4.0E-17	223	290	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	15.618	223	273	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	2.3E-7	227	274	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	2.3E-9	229	279	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006351	Biological Process	transcription, DNA-templated
GO:0009637	Biological Process	response to blue light
GO:0009911	Biological Process	positive regulation of flower development
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 423 aa Download sequence Send to blast
MNRALAETLT VLDRQRARVK WQQESYFSEL SGVFSNQTST HVHGFQGDLI SDESVLDDLV 60 MTRQVKPDPS LETSWPELGK VDMAGMGFGP CGYSNGPSFD MNYAISRTFS CPPAVAATIA 120 KEAMEVKGKE SIVSENMGSA VARESSKKRK ADKLHNSKVA AEDDSKKTKA CGEEEEESKI 180 TGPPNTNKSS TKQEPSADTS KENSKLTEVQ KPDYIHVRAR RGQATDSHSL AERVRREKIS 240 ERMKYLQDLV PGCNKITGKA GMLDEIINYV QSLQRQVEFL SMKLAAVNPR LDFDIDNLFA 300 KDVFPPCMTN FPTVGMSSEM ANPSYLHFNP VQQVVACSGV EMGLNSPDIA LRRTISAPKS 360 TIPDASFLDT SCFTQIQPSP TWDVELQNVY NVAFEHGRST TPFPSQPFAA AGSIEASHLK 420 MEM

Sequence ? help Back to Top

Protein Sequence Length: 423 aa Download sequence Send to blast

MNRALAETLT VLDRQRARVK WQQESYFSEL SGVFSNQTST HVHGFQGDLI SDESVLDDLV  60
MTRQVKPDPS LETSWPELGK VDMAGMGFGP CGYSNGPSFD MNYAISRTFS CPPAVAATIA  120
KEAMEVKGKE SIVSENMGSA VARESSKKRK ADKLHNSKVA AEDDSKKTKA CGEEEEESKI  180
TGPPNTNKSS TKQEPSADTS KENSKLTEVQ KPDYIHVRAR RGQATDSHSL AERVRREKIS  240
ERMKYLQDLV PGCNKITGKA GMLDEIINYV QSLQRQVEFL SMKLAAVNPR LDFDIDNLFA  300
KDVFPPCMTN FPTVGMSSEM ANPSYLHFNP VQQVVACSGV EMGLNSPDIA LRRTISAPKS  360
TIPDASFLDT SCFTQIQPSP TWDVELQNVY NVAFEHGRST TPFPSQPFAA AGSIEASHLK  420
MEM

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, and stems. {ECO:0000269\|PubMed:12679534}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor that binds DNA to G box 5'-CACGTG-3' and, to a lower extent, to E-box 5'-CANNTG-3' in vitro. Binds to chromatin DNA of the FT gene and promotes its expression, and thus triggers flowering in response to blue light. {ECO:0000269\|PubMed:18988809, ECO:0000269\|PubMed:24130508}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Accumulates strongly in response to blue light due to reduced preventing 26S proteasome-mediated degradation in an ADO1/ZTL and ADO2/LKP2 dependent manner, but levels decrease in the absence of blue light via 26S proteasome degradation (at protein level). {ECO:0000269\|PubMed:24101505}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_016734342.1	0.0	PREDICTED: transcription factor bHLH63-like isoform X2
Swissprot	Q8GY61	3e-66	BH063_ARATH; Transcription factor bHLH63
TrEMBL	A0A1U8N5G6	0.0	A0A1U8N5G6_GOSHI; transcription factor bHLH63-like isoform X2
STRING	Gorai.008G029300.1	0.0	(Gossypium raimondii)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM5947	25	43

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G34530.1	1e-68	cryptochrome-interacting basic-helix-loop-helix 1

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Yang D,Zhao W,Meng Y,Li H,Liu B A CIB1-LIKE transcription factor GmCIL10 from soybean positively regulates plant flowering. Sci China Life Sci, 2015. 58(3): p. 261-9 [PMID:25651969] Katsura Y, et al. An optogenetic system for interrogating the temporal dynamics of Akt. Sci Rep, 2015. 5: p. 14589 [PMID:26423353] Taslimi A, et al. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Nat. Chem. Biol., 2016. 12(6): p. 425-30 [PMID:27065233] Liu Q, et al. The Blue Light-Dependent Polyubiquitination and Degradation of Arabidopsis Cryptochrome2 Requires Multiple E3 Ubiquitin Ligases. Plant Cell Physiol., 2016. 57(10): p. 2175-2186 [PMID:27516416] Pathak GP, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res., 2017. 45(20): p. e167 [PMID:28431041] Duan L, et al. Understanding CRY2 interactions for optical control of intracellular signaling. Nat Commun, 2017. 8(1): p. 547 [PMID:28916751] Quejada JR, et al. Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1. Nucleic Acids Res., 2017. 45(20): p. e172 [PMID:29040770] Liu Y, et al. CIB1 and CO interact to mediate CRY2-dependent regulation of flowering. EMBO Rep., 2019. [PMID:30126927]