PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Gh_D04G0466

Organism

Gossypium hirsutum

Taxonomic ID

3635

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium

Family

AP2

Protein Properties

Length: 432aa MW: 49565.6 Da PI: 9.4336

Description

AP2 family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Gh_D04G0466	genome	NAU-NBI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	23.6	1.3e-07	102	151	8	55
AP2 8 wdkkrgrWvAeIrd.pseng..krkrfslgkfgtaeeAakaaiaarkkleg 55 +++++gr++A+++d s+n k+k + g ++ +e Aa+a++ a++k++g Gh_D04G0466 102 RHRWTGRYEAHLWDkLSWNItqKKKGKQ-GAYDEEEAAARAYDLAALKYWG 151 799*********88887784446666.9*****************98 PP
2	AP2	26.3	1.9e-08	194	226	1	36
AP2 1 sgykGVrwdkkrgrWvAeIrd.psengkrkrfslgkf 36 s+y+GV +++++grW+A+I + g +k+ +lg++ Gh_D04G0466 194 SKYRGVARHHNNGRWEARIGRvF---G-NKYLYLGTY 226 89**************99966...3.6*****9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS51032	16.186	71	159	IPR001471	AP2/ERF domain
Pfam	PF00847	1.5E-4	102	151	IPR001471	AP2/ERF domain
SMART	SM00380	7.9E-11	102	165	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	1.7E-9	102	160	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	3.01E-11	102	161	IPR016177	DNA-binding domain
SuperFamily	SSF54171	2.55E-5	194	228	IPR016177	DNA-binding domain
Pfam	PF00847	1.4E-4	194	226	IPR001471	AP2/ERF domain
CDD	cd00018	3.31E-19	194	281	No hit	No description
SMART	SM00380	1.6E-15	195	285	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	6.3E-4	195	227	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	15.198	195	279	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	1.18E-5	247	281	IPR016177	DNA-binding domain
Gene3D	G3DSA:3.30.730.10	1.4E-6	254	280	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 432 aa Download sequence Send to blast
MEMRMVNDER YLGLGRRRKC CDDSENIQVE AIRCAKRRRR DPAGSVAFDN RDGHQQRNAA 60 ATATTVKRSS RFRGVSRYCK IKQVFGDIQN YNNHRINMKM NRHRWTGRYE AHLWDKLSWN 120 ITQKKKGKQG AYDEEEAAAR AYDLAALKYW GTSTFTNFPI SDYGKEIEIM QTLTKEEYLA 180 SLRRKSSGFS RGVSKYRGVA RHHNNGRWEA RIGRVFGNKY LYLGTYSKFH IWTPGRYIFV 240 SNTYTGLYIS FTGTQEEAAR AYDIAAIEYR GINAVTNFDL STYIRWLKPN ESLPMAVEPE 300 PVTLPSQAST PREESKPSFN HSSTADYLNS FPKQVVESKI HVMNSNKCSS TTALGLLLRS 360 SIFRDLVEKN LANVCEDESG STDEDEEKNK HLAGNDDEFC GLFYDGIGTG FPFFRSSMKD 420 TMELQERGSS FI

Sequence ? help Back to Top

Protein Sequence Length: 432 aa Download sequence Send to blast

MEMRMVNDER YLGLGRRRKC CDDSENIQVE AIRCAKRRRR DPAGSVAFDN RDGHQQRNAA  60
ATATTVKRSS RFRGVSRYCK IKQVFGDIQN YNNHRINMKM NRHRWTGRYE AHLWDKLSWN  120
ITQKKKGKQG AYDEEEAAAR AYDLAALKYW GTSTFTNFPI SDYGKEIEIM QTLTKEEYLA  180
SLRRKSSGFS RGVSKYRGVA RHHNNGRWEA RIGRVFGNKY LYLGTYSKFH IWTPGRYIFV  240
SNTYTGLYIS FTGTQEEAAR AYDIAAIEYR GINAVTNFDL STYIRWLKPN ESLPMAVEPE  300
PVTLPSQAST PREESKPSFN HSSTADYLNS FPKQVVESKI HVMNSNKCSS TTALGLLLRS  360
SIFRDLVEKN LANVCEDESG STDEDEEKNK HLAGNDDEFC GLFYDGIGTG FPFFRSSMKD  420
TMELQERGSS FI

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	16	38	RRKCCDDSENIQVEAIRCAKRRR

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Mostly expressed in siliques, especially in seeds. Also detected in roots and flowers, and, to a lower extent, in leaves stems and seedlings. {ECO:0000269\|PubMed:15500472, ECO:0000269\|PubMed:15753106}.

Functional Description ? help Back to Top
Source	Description
UniProt	May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds. Binds to the GCC-box pathogenesis-related promoter element. Promotes sugar uptake and seed oil accumulation by glycolysis. Required for embryo development, seed germination and, indirectly, for seedling establishment. Negative regulator of the ABA-mediated germination inhibition. {ECO:0000250, ECO:0000269\|PubMed:12084821, ECO:0000269\|PubMed:15500472, ECO:0000269\|PubMed:15753106, ECO:0000269\|PubMed:16553903, ECO:0000269\|PubMed:16632590, ECO:0000269\|PubMed:9733529}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Transiantly in leaves by sucrose, but not by abscisic acid (ABA). {ECO:0000269\|PubMed:15753106}.

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	JX578540	9e-32	JX578540.1 Gossypium hirsutum clone NBRI_GE10584 microsatellite sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_016752994.1	0.0	PREDICTED: AP2-like ethylene-responsive transcription factor At1g79700
Swissprot	Q6X5Y6	6e-76	WRI1_ARATH; Ethylene-responsive transcription factor WRI1
TrEMBL	A0A1U8PRV0	0.0	A0A1U8PRV0_GOSHI; AP2-like ethylene-responsive transcription factor At1g79700
STRING	Gorai.012G056300.1	0.0	(Gossypium raimondii)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM553	27	140

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G54320.3	1e-65	AP2 family protein

Publications ? help Back to Top
Kim HU, et al. Ectopic overexpression of castor bean LEAFY COTYLEDON2 (LEC2) in Arabidopsis triggers the expression of genes that encode regulators of seed maturation and oil body proteins in vegetative tissues. FEBS Open Bio, 2013. 4: p. 25-32 [PMID:24363987] Wu XL,Liu ZH,Hu ZH,Huang RZ BnWRI1 coordinates fatty acid biosynthesis and photosynthesis pathways during oil accumulation in rapeseed. J Integr Plant Biol, 2014. 56(6): p. 582-93 [PMID:24393360] Shigematsu H, et al. Structural characterization of the mechanosensitive channel candidate MCA2 from Arabidopsis thaliana. PLoS ONE, 2014. 9(1): p. e87724 [PMID:24475319] Kim HU, et al. Senescence-inducible LEC2 enhances triacylglycerol accumulation in leaves without negatively affecting plant growth. Plant Biotechnol. J., 2015. 13(9): p. 1346-59 [PMID:25790072] Grimberg Å,Carlsson AS,Marttila S,Bhalerao R,Hofvander P Transcriptional transitions in Nicotiana benthamiana leaves upon induction of oil synthesis by WRINKLED1 homologs from diverse species and tissues. BMC Plant Biol., 2015. 15: p. 192 [PMID:26253704] Kanai M,Mano S,Kondo M,Hayashi M,Nishimura M Extension of oil biosynthesis during the mid-phase of seed development enhances oil content in Arabidopsis seeds. Plant Biotechnol. J., 2016. 14(5): p. 1241-50 [PMID:26503031] Li Q, et al. Wrinkled1 Accelerates Flowering and Regulates Lipid Homeostasis between Oil Accumulation and Membrane Lipid Anabolism in Brassica napus. Front Plant Sci, 2015. 6: p. 1015 [PMID:26635841] Hofvander P, et al. Potato tuber expression of Arabidopsis WRINKLED1 increase triacylglycerol and membrane lipids while affecting central carbohydrate metabolism. Plant Biotechnol. J., 2016. 14(9): p. 1883-98 [PMID:26914183] Shen SL, et al. CitAP2.10 activation of the terpene synthase CsTPS1 is associated with the synthesis of (+)-valencene in 'Newhall' orange. J. Exp. Bot., 2016. 67(14): p. 4105-15 [PMID:27194737] Adhikari ND,Bates PD,Browse J WRINKLED1 Rescues Feedback Inhibition of Fatty Acid Synthesis in Hydroxylase-Expressing Seeds. Plant Physiol., 2016. 171(1): p. 179-91 [PMID:27208047] Kim MJ,Jang IC,Chua NH The Mediator Complex MED15 Subunit Mediates Activation of Downstream Lipid-Related Genes by the WRINKLED1 Transcription Factor. Plant Physiol., 2016. 171(3): p. 1951-64 [PMID:27246098] Bhattacharya S,Das N,Maiti MK Cumulative effect of heterologous AtWRI1 gene expression and endogenous BjAGPase gene silencing increases seed lipid content in Indian mustard Brassica juncea. Plant Physiol. Biochem., 2016. 107: p. 204-213 [PMID:27314514] Ma W, et al. 14-3-3 protein mediates plant seed oil biosynthesis through interaction with AtWRI1. Plant J., 2016. 88(2): p. 228-235 [PMID:27322486] Li D, et al. MYB89 Transcription Factor Represses Seed Oil Accumulation. Plant Physiol., 2017. 173(2): p. 1211-1225 [PMID:27932421] Ivarson E, et al. Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre. Front Plant Sci, 2016. 7: p. 2032 [PMID:28119714] An D, et al. Expression of Camelina WRINKLED1 Isoforms Rescue the Seed Phenotype of the Arabidopsis wri1 Mutant and Increase the Triacylglycerol Content in Tobacco Leaves. Front Plant Sci, 2017. 8: p. 34 [PMID:28174580] Zhai Z,Liu H,Shanklin J Phosphorylation of WRINKLED1 by KIN10 Results in Its Proteasomal Degradation, Providing a Link between Energy Homeostasis and Lipid Biosynthesis. Plant Cell, 2017. 29(4): p. 871-889 [PMID:28314829] Jin J, et al. Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm. Sci Rep, 2017. 7(1): p. 439 [PMID:28348403] Kong Q, et al. The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots. J. Exp. Bot., 2017. 68(16): p. 4627-4634 [PMID:28981783] Kang NK, et al. Increased lipid production by heterologous expression of AtWRI1 transcription factor in Nannochloropsis salina. Biotechnol Biofuels, 2017. 10: p. 231 [PMID:29046718] Pellaud S, et al. WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis. New Phytol., 2018. 217(1): p. 245-260 [PMID:29105089] Hanano A,Almousally I,Shaban M,Murphy DJ Arabidopsis plants exposed to dioxin result in a WRINKLED seed phenotype due to 20S proteasomal degradation of WRI1. J. Exp. Bot., 2018. 69(7): p. 1781-1794 [PMID:29394403]