PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

462877146

Organism

Eragrostis tef

Taxonomic ID

110835

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Chloridoideae; Eragrostideae; Eragrostidinae; Eragrostis

Family

AP2

Protein Properties

Length: 636aa MW: 70264 Da PI: 9.9854

Description

AP2 family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
462877146	genome	Tef	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	46.8	7.5e-15	70	127	1	53
AP2 1 sgykGVrwdkkrgrWvAeIrd.pseng...kr.krfslgkfgtaeeAakaaiaarkkl 53 s+++GV++++++gr++A+++d s+n k+ k+++lg ++ +e Aa+a++ a++k+ 462877146 70 SRFRGVSRHRWTGRFEAHLWDkNSWNPtqrKKgKQVYLGAYDEEEAAARAYDLAALKK 127 789****************888855666447********************986 PP
2	AP2	50.4	5.4e-16	136	187	1	55
AP2 1 sgykGVrwdkkrgrWvAeIrd.psengkrkrfslgkfgtaeeAakaaiaarkkleg 55 s+y+GV +++++grW+A+I + g +k+ +lg++ t eeAa+a++ a+ +++g 462877146 136 SKYRGVARHHQNGRWEARIGRvF---G-NKYLYLGTYSTQEEAARAYDIAAIEYKG 187 89**************99966...3.6*********************9986 PP
3	AP2	49.6	9.8e-16	305	364	1	55
AP2 1 sgykGVrwdkkrgrWvAeIrd.pseng...kr.krfslgkfgtaeeAakaaiaarkkleg 55 s+++GV++++++gr++A+++d s+n k+ k+++lg ++ +e Aa+a++ a++k++g 462877146 305 SRFRGVSRHRWTGRFEAHLWDkNSWNPtqrKKgKQVYLGAYDEEEAAARAYDLAALKYWG 364 789****************888855666447***********************98 PP
4	AP2	50.4	5.4e-16	407	458	1	55
AP2 1 sgykGVrwdkkrgrWvAeIrd.psengkrkrfslgkfgtaeeAakaaiaarkkleg 55 s+y+GV +++++grW+A+I + g +k+ +lg++ t eeAa+a++ a+ +++g 462877146 407 SKYRGVARHHQNGRWEARIGRvF---G-NKYLYLGTYSTQEEAARAYDIAAIEYKG 458 89**************99966...3.6*********************9986 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF54171	2.29E-11	70	128	IPR016177	DNA-binding domain
Pfam	PF00847	5.1E-11	70	127	IPR001471	AP2/ERF domain
SMART	SM00380	1.4E-10	71	129	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	15.975	71	126	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	6.9E-11	71	126	IPR001471	AP2/ERF domain
Pfam	PF00847	6.8E-11	136	187	IPR001471	AP2/ERF domain
CDD	cd00018	2.31E-22	136	195	No hit	No description
SuperFamily	SSF54171	5.04E-17	136	196	IPR016177	DNA-binding domain
SMART	SM00380	1.7E-26	137	201	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	2.8E-17	137	195	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	18.979	137	195	IPR001471	AP2/ERF domain
Pfam	PF00847	3.1E-12	305	364	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	2.22E-16	305	373	IPR016177	DNA-binding domain
SMART	SM00380	1.1E-24	306	378	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	1.9E-15	306	373	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	19.809	306	372	IPR001471	AP2/ERF domain
Pfam	PF00847	6.8E-11	407	458	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	5.04E-17	407	467	IPR016177	DNA-binding domain
CDD	cd00018	2.31E-22	407	466	No hit	No description
SMART	SM00380	1.7E-26	408	472	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	2.8E-17	408	466	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	18.979	408	466	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006110	Biological Process	regulation of glycolytic process
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009744	Biological Process	response to sucrose
GO:0019432	Biological Process	triglyceride biosynthetic process
GO:1901959	Biological Process	positive regulation of cutin biosynthetic process
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 636 aa Download sequence Send to blast
MENMVMVKNE IENFSGSAPA VMINASEGGE ASQVVRRRRR EPALLAPISG GANSSGIGKP 60 LPSITVKRSS RFRGVSRHRW TGRFEAHLWD KNSWNPTQRK KGKQVYLGAY DEEEAAARAY 120 DLAALKKSSG FSRGVSKYRG VARHHQNGRW EARIGRVFGN KYLYLGTYST QEEAARAYDI 180 AAIEYKGVNA VTNFDLRSYI TWLKPSTPLQ LLLRSFRAAA ISASEAAFGA GSVAVMENMV 240 MVKNEIENFS GSAPAVMINA SEGGEASQVV RRRRREPALL APISGGANSS GIGKPLPSIT 300 VKRSSRFRGV SRHRWTGRFE AHLWDKNSWN PTQRKKGKQV YLGAYDEEEA AARAYDLAAL 360 KYWGPTTYTN FPVMDYEKEL KIMENLTKEE YLASLRRKSS GFSRGVSKYR GVARHHQNGR 420 WEARIGRVFG NKYLYLGTYS TQEEAARAYD IAAIEYKGVN AVTNFDLRSY ITWLKPSTPV 480 TFNPEALVMQ ATPAEQVLQT ETQMLPRGNP FLLDHNALPG SSSISQEASM ISPGGMRKRG 540 SSTALSLLFK SSMFRQLVEK NSDAEEAAGG VREAGAHPGE AYEYHNFFQA DGPDMCDLFS 600 SGCGNAPNAF HGQIACYDDG ESAATWNGFG NMSSLP

Sequence ? help Back to Top

Protein Sequence Length: 636 aa Download sequence Send to blast

MENMVMVKNE IENFSGSAPA VMINASEGGE ASQVVRRRRR EPALLAPISG GANSSGIGKP  60
LPSITVKRSS RFRGVSRHRW TGRFEAHLWD KNSWNPTQRK KGKQVYLGAY DEEEAAARAY  120
DLAALKKSSG FSRGVSKYRG VARHHQNGRW EARIGRVFGN KYLYLGTYST QEEAARAYDI  180
AAIEYKGVNA VTNFDLRSYI TWLKPSTPLQ LLLRSFRAAA ISASEAAFGA GSVAVMENMV  240
MVKNEIENFS GSAPAVMINA SEGGEASQVV RRRRREPALL APISGGANSS GIGKPLPSIT  300
VKRSSRFRGV SRHRWTGRFE AHLWDKNSWN PTQRKKGKQV YLGAYDEEEA AARAYDLAAL  360
KYWGPTTYTN FPVMDYEKEL KIMENLTKEE YLASLRRKSS GFSRGVSKYR GVARHHQNGR  420
WEARIGRVFG NKYLYLGTYS TQEEAARAYD IAAIEYKGVN AVTNFDLRSY ITWLKPSTPV  480
TFNPEALVMQ ATPAEQVLQT ETQMLPRGNP FLLDHNALPG SSSISQEASM ISPGGMRKRG  540
SSTALSLLFK SSMFRQLVEK NSDAEEAAGG VREAGAHPGE AYEYHNFFQA DGPDMCDLFS  600
SGCGNAPNAF HGQIACYDDG ESAATWNGFG NMSSLP

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	330	338	PTQRKKGKQ

Functional Description ? help Back to Top
Source	Description
UniProt	May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds. Binds to the GCC-box pathogenesis-related promoter element. Promotes sugar uptake and seed oil accumulation by glycolysis. Required for embryo development, seed germination and, indirectly, for seedling establishment. Negative regulator of the ABA-mediated germination inhibition. {ECO:0000250, ECO:0000269\|PubMed:12084821, ECO:0000269\|PubMed:15500472, ECO:0000269\|PubMed:15753106, ECO:0000269\|PubMed:16553903, ECO:0000269\|PubMed:16632590, ECO:0000269\|PubMed:9733529}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	462877146

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Transiantly in leaves by sucrose, but not by abscisic acid (ABA). {ECO:0000269\|PubMed:15753106}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY685112	1e-169	AY685112.1 Oryza sativa (japonica cultivar-group) transcription factor AP2D4 (AP2D4) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_004961382.1	0.0	ethylene-responsive transcription factor WRI1
Swissprot	Q6X5Y6	2e-87	WRI1_ARATH; Ethylene-responsive transcription factor WRI1
TrEMBL	A0A1E5WDW3	0.0	A0A1E5WDW3_9POAL; Ethylene-responsive transcription factor WRI1
STRING	Si022186m	0.0	(Setaria italica)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP4162	34	73

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G54320.1	4e-77	AP2 family protein

Publications ? help Back to Top
Kim HU, et al. Ectopic overexpression of castor bean LEAFY COTYLEDON2 (LEC2) in Arabidopsis triggers the expression of genes that encode regulators of seed maturation and oil body proteins in vegetative tissues. FEBS Open Bio, 2013. 4: p. 25-32 [PMID:24363987] Wu XL,Liu ZH,Hu ZH,Huang RZ BnWRI1 coordinates fatty acid biosynthesis and photosynthesis pathways during oil accumulation in rapeseed. J Integr Plant Biol, 2014. 56(6): p. 582-93 [PMID:24393360] Shigematsu H, et al. Structural characterization of the mechanosensitive channel candidate MCA2 from Arabidopsis thaliana. PLoS ONE, 2014. 9(1): p. e87724 [PMID:24475319] Kim HU, et al. Senescence-inducible LEC2 enhances triacylglycerol accumulation in leaves without negatively affecting plant growth. Plant Biotechnol. J., 2015. 13(9): p. 1346-59 [PMID:25790072] Grimberg Å,Carlsson AS,Marttila S,Bhalerao R,Hofvander P Transcriptional transitions in Nicotiana benthamiana leaves upon induction of oil synthesis by WRINKLED1 homologs from diverse species and tissues. BMC Plant Biol., 2015. 15: p. 192 [PMID:26253704] Kanai M,Mano S,Kondo M,Hayashi M,Nishimura M Extension of oil biosynthesis during the mid-phase of seed development enhances oil content in Arabidopsis seeds. Plant Biotechnol. J., 2016. 14(5): p. 1241-50 [PMID:26503031] Li Q, et al. Wrinkled1 Accelerates Flowering and Regulates Lipid Homeostasis between Oil Accumulation and Membrane Lipid Anabolism in Brassica napus. Front Plant Sci, 2015. 6: p. 1015 [PMID:26635841] Hofvander P, et al. Potato tuber expression of Arabidopsis WRINKLED1 increase triacylglycerol and membrane lipids while affecting central carbohydrate metabolism. Plant Biotechnol. J., 2016. 14(9): p. 1883-98 [PMID:26914183] Shen SL, et al. CitAP2.10 activation of the terpene synthase CsTPS1 is associated with the synthesis of (+)-valencene in 'Newhall' orange. J. Exp. Bot., 2016. 67(14): p. 4105-15 [PMID:27194737] Adhikari ND,Bates PD,Browse J WRINKLED1 Rescues Feedback Inhibition of Fatty Acid Synthesis in Hydroxylase-Expressing Seeds. Plant Physiol., 2016. 171(1): p. 179-91 [PMID:27208047] Kim MJ,Jang IC,Chua NH The Mediator Complex MED15 Subunit Mediates Activation of Downstream Lipid-Related Genes by the WRINKLED1 Transcription Factor. Plant Physiol., 2016. 171(3): p. 1951-64 [PMID:27246098] Bhattacharya S,Das N,Maiti MK Cumulative effect of heterologous AtWRI1 gene expression and endogenous BjAGPase gene silencing increases seed lipid content in Indian mustard Brassica juncea. Plant Physiol. Biochem., 2016. 107: p. 204-213 [PMID:27314514] Ma W, et al. 14-3-3 protein mediates plant seed oil biosynthesis through interaction with AtWRI1. Plant J., 2016. 88(2): p. 228-235 [PMID:27322486] Li D, et al. MYB89 Transcription Factor Represses Seed Oil Accumulation. Plant Physiol., 2017. 173(2): p. 1211-1225 [PMID:27932421] Ivarson E, et al. Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre. Front Plant Sci, 2016. 7: p. 2032 [PMID:28119714] An D, et al. Expression of Camelina WRINKLED1 Isoforms Rescue the Seed Phenotype of the Arabidopsis wri1 Mutant and Increase the Triacylglycerol Content in Tobacco Leaves. Front Plant Sci, 2017. 8: p. 34 [PMID:28174580] Zhai Z,Liu H,Shanklin J Phosphorylation of WRINKLED1 by KIN10 Results in Its Proteasomal Degradation, Providing a Link between Energy Homeostasis and Lipid Biosynthesis. Plant Cell, 2017. 29(4): p. 871-889 [PMID:28314829] Jin J, et al. Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm. Sci Rep, 2017. 7(1): p. 439 [PMID:28348403] Kong Q, et al. The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots. J. Exp. Bot., 2017. 68(16): p. 4627-4634 [PMID:28981783] Kang NK, et al. Increased lipid production by heterologous expression of AtWRI1 transcription factor in Nannochloropsis salina. Biotechnol Biofuels, 2017. 10: p. 231 [PMID:29046718] Pellaud S, et al. WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis. New Phytol., 2018. 217(1): p. 245-260 [PMID:29105089] Hanano A,Almousally I,Shaban M,Murphy DJ Arabidopsis plants exposed to dioxin result in a WRINKLED seed phenotype due to 20S proteasomal degradation of WRI1. J. Exp. Bot., 2018. 69(7): p. 1781-1794 [PMID:29394403]