PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

DCAR_031998

Organism

Daucus carota

Taxonomic ID

4039

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Apiales; Apiineae; Apiaceae; Apioideae; Scandiceae; Daucinae; Daucus

Family

bHLH

Protein Properties

Length: 425aa MW: 46407.4 Da PI: 6.629

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
DCAR_031998	genome	ARS-USDA	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	13.2	0.00016	341	371	21	55
HHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 21 eeLrellPkaskapskKlsKaeiLekAveYIksLq 55 ++L +lP++ p K Ka+i+e A +YIk L+ DCAR_031998 341 ARLHAMLPHL---P-AKSGKATIVEAAANYIKTLE 371 68999**6...3.88999**********995 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:3.90.550.10	7.4E-73	79	303	IPR029044	Nucleotide-diphospho-sugar transferases
Hamap	MF_00108	27.78	81	304	IPR001228	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
CDD	cd02516	8.05E-87	82	297	No hit	No description
SuperFamily	SSF53448	2.71E-52	82	302	IPR029044	Nucleotide-diphospho-sugar transferases
Pfam	PF01128	3.7E-53	82	302	IPR001228	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
TIGRFAMs	TIGR00453	5.6E-63	83	301	IPR001228	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
SuperFamily	SSF47459	2.62E-6	338	382	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	1.6E-6	338	384	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0008299	Biological Process	isoprenoid biosynthetic process
GO:0046983	Molecular Function	protein dimerization activity
GO:0050518	Molecular Function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity

Sequence ? help Back to Top
Protein Sequence Length: 425 aa Download sequence Send to blast
MSALKFSCCP QLNSLEISSK SLNSLPNSKI FLPVSKNCSF VNTHYPKILS LRNGGASSRI 60 SCSASSSELS KDYDEFVEKK SVSVILLAGG KGKRMGASMP KQYLPLFGQP IALYSFYTFS 120 LLPEVKEIIV VCDPSYQDIF EDAKENTHVD LKFALPGKER QDSVYSGLQV VDPNSSLLCI 180 HDSARPLVSS SDTKKVLIDG WLTGAAVLGV PAKATIKEAN MESFVVKTLD RKTLWEVQTP 240 QVMKPGLLKK GFELVNREGL EVTDDVSIVE HLKHPVYITE GSYTNIKVTT PDDMILAEKI 300 LNATSSCDAS SSATSFGNEV VPVPKPSSEQ EYIVLKTEEV ARLHAMLPHL PAKSGKATIV 360 EAAANYIKTL EDEVKKLQTL KQEAVNGTET MKSNLPSAAN QEMPRVEMME TFVANQVLLP 420 KINL*

Sequence ? help Back to Top

Protein Sequence Length: 425 aa Download sequence Send to blast

MSALKFSCCP QLNSLEISSK SLNSLPNSKI FLPVSKNCSF VNTHYPKILS LRNGGASSRI  60
SCSASSSELS KDYDEFVEKK SVSVILLAGG KGKRMGASMP KQYLPLFGQP IALYSFYTFS  120
LLPEVKEIIV VCDPSYQDIF EDAKENTHVD LKFALPGKER QDSVYSGLQV VDPNSSLLCI  180
HDSARPLVSS SDTKKVLIDG WLTGAAVLGV PAKATIKEAN MESFVVKTLD RKTLWEVQTP  240
QVMKPGLLKK GFELVNREGL EVTDDVSIVE HLKHPVYITE GSYTNIKVTT PDDMILAEKI  300
LNATSSCDAS SSATSFGNEV VPVPKPSSEQ EYIVLKTEEV ARLHAMLPHL PAKSGKATIV  360
EAAANYIKTL EDEVKKLQTL KQEAVNGTET MKSNLPSAAN QEMPRVEMME TFVANQVLLP  420
KINL*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1w77_A	1e-126	79	305	2	228	2C-METHYL-D-ERYTHRITOL 4-PHOSPHATE CYTIDYLYLTRANSFERASE
4nai_A	1e-126	79	305	2	228	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic
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Functional Description ? help Back to Top
Source	Description
UniProt	Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP). Is essential for chloroplast development and required for pigments and gibberellins biosynthesis. {ECO:0000269\|PubMed:10841550, ECO:0000269\|PubMed:12029484, ECO:0000269\|PubMed:18236010}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Circadian-regulated with a peak in the late period of the light phase. {ECO:0000269\|PubMed:15863698, ECO:0000269\|PubMed:18236010}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_017228499.1	0.0	PREDICTED: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic-like
Swissprot	P69834	1e-122	ISPD_ARATH; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic
TrEMBL	A0A175YAK5	0.0	A0A175YAK5_DAUCS; 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
STRING	XP_009607716.1	1e-148	(Nicotiana tomentosiformis)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G49770.1	4e-06	bHLH family protein

Link Out ? help Back to Top
Phytozome	DCAR_031998

Publications ? help Back to Top
Rohdich F, et al. Biosynthesis of terpenoids: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase of Arabidopsis thaliana. Proc. Natl. Acad. Sci. U.S.A., 2000. 97(12): p. 6451-6 [PMID:10841550] Okada K, et al. Antisense and chemical suppression of the nonmevalonate pathway affects ent-kaurene biosynthesis in Arabidopsis. Planta, 2002. 215(2): p. 339-44 [PMID:12029484] Lange BM,Ghassemian M Genome organization in Arabidopsis thaliana: a survey for genes involved in isoprenoid and chlorophyll metabolism. Plant Mol. Biol., 2003. 51(6): p. 925-48 [PMID:12777052] Hsieh MH,Goodman HM The Arabidopsis IspH homolog is involved in the plastid nonmevalonate pathway of isoprenoid biosynthesis. Plant Physiol., 2005. 138(2): p. 641-53 [PMID:15863698] Peltier JB, et al. The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts. Mol. Cell Proteomics, 2006. 5(1): p. 114-33 [PMID:16207701] Gabrielsen M, et al. The crystal structure of a plant 2C-methyl-D-erythritol 4-phosphate cytidylyltransferase exhibits a distinct quaternary structure compared to bacterial homologues and a possible role in feedback regulation for cytidine monophosphate. FEBS J., 2006. 273(5): p. 1065-73 [PMID:16478479] Kim SM,Kuzuyama T,Chang YJ,Kwon HJ,Kim SU Cloning and functional characterization of 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (GbMECT) gene from Ginkgo biloba. Phytochemistry, 2006. 67(14): p. 1435-41 [PMID:16828818] Hsieh MH,Chang CY,Hsu SJ,Chen JJ Chloroplast localization of methylerythritol 4-phosphate pathway enzymes and regulation of mitochondrial genes in ispD and ispE albino mutants in Arabidopsis. Plant Mol. Biol., 2008. 66(6): p. 663-73 [PMID:18236010] Rutschow H,Ytterberg AJ,Friso G,Nilsson R,van Wijk KJ Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis. Plant Physiol., 2008. 148(1): p. 156-75 [PMID:18633119] Phillips MA,León P,Boronat A,Rodríguez-Concepción M The plastidial MEP pathway: unified nomenclature and resources. Trends Plant Sci., 2008. 13(12): p. 619-23 [PMID:18948055] Bryant N,Lloyd J,Sweeney C,Myouga F,Meinke D Identification of nuclear genes encoding chloroplast-localized proteins required for embryo development in Arabidopsis. Plant Physiol., 2011. 155(4): p. 1678-89 [PMID:21139083] Witschel MC, et al. Inhibitors of the herbicidal target IspD: allosteric site binding. Angew. Chem. Int. Ed. Engl., 2011. 50(34): p. 7931-5 [PMID:21766403] Kunfermann A, et al. Pseudilins: halogenated, allosteric inhibitors of the non-mevalonate pathway enzyme IspD. Angew. Chem. Int. Ed. Engl., 2014. 53(8): p. 2235-9 [PMID:24446431] Schwab A, et al. Mechanism of Allosteric Inhibition of the Enzyme IspD by Three Different Classes of Ligands. ACS Chem. Biol., 2017. 12(8): p. 2132-2138 [PMID:28686408]