PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

MELO3C006315P1

Organism

Cucumis melo

Taxonomic ID

3656

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis

Family

bHLH

Protein Properties

Length: 379aa MW: 42271.1 Da PI: 4.902

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
MELO3C006315P1	genome	MELONOMICS	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	38.2	2.6e-12	178	229	1	55
CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +r++h ++Er+RR+++N+ + Lr+l+P + + ++ + a+i A+e++++L+ MELO3C006315P1 178 QRMTHIAVERNRRKQMNEHLRVLRSLMPGS---YVQRGDQASIIGGAIEFVRELE 229 79**************************...9***************9995 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
CDD	cd00083	3.61E-13	176	233	No hit	No description
Gene3D	G3DSA:4.10.280.10	1.3E-13	176	232	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	1.31E-16	177	244	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	15.428	177	228	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	1.2E-9	178	229	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	3.4E-9	183	234	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd04873	0.00206	304	368	No hit	No description
PROSITE profile	PS51671	8.573	304	378	IPR002912	ACT domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0010377	Biological Process	guard cell fate commitment
GO:0045597	Biological Process	positive regulation of cell differentiation
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0051782	Biological Process	negative regulation of cell division
GO:0005634	Cellular Component	nucleus
GO:0016597	Molecular Function	amino acid binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 379 aa Download sequence Send to blast
MHLVNSMEKD DNYSNEGSMA PSFSGHQDLY NNQDQFMRSR PNFETSADNS TGILTYFPAN 60 PITSPDKLSF GEVMQFADFG PKLSLNRFDD DSEPPGIDPV YFLKFPVLNN ERVEETATTG 120 GETEEAAEIG GRSLNIVGEV EEENFRNNNN NNNNNNGNNS NKRKRGRTVK TSEEVESQRM 180 THIAVERNRR KQMNEHLRVL RSLMPGSYVQ RGDQASIIGG AIEFVRELEQ LLQCLESQKR 240 RRLLGESPIV QAADTPPPQQ PPFLPPGASN FAGHQNDGQI FETTAESKSC LADVEVKVIG 300 FDAMIKILSR RRPGQLIKAI AALEDLQLNI LHTNITTIEQ TVLYSFNVKI ASESRFTAED 360 IASSVQQIFN FIHANTGM*

Sequence ? help Back to Top

Protein Sequence Length: 379 aa Download sequence Send to blast

MHLVNSMEKD DNYSNEGSMA PSFSGHQDLY NNQDQFMRSR PNFETSADNS TGILTYFPAN  60
PITSPDKLSF GEVMQFADFG PKLSLNRFDD DSEPPGIDPV YFLKFPVLNN ERVEETATTG  120
GETEEAAEIG GRSLNIVGEV EEENFRNNNN NNNNNNGNNS NKRKRGRTVK TSEEVESQRM  180
THIAVERNRR KQMNEHLRVL RSLMPGSYVQ RGDQASIIGG AIEFVRELEQ LLQCLESQKR  240
RRLLGESPIV QAADTPPPQQ PPFLPPGASN FAGHQNDGQI FETTAESKSC LADVEVKVIG  300
FDAMIKILSR RRPGQLIKAI AALEDLQLNI LHTNITTIEQ TVLYSFNVKI ASESRFTAED  360
IASSVQQIFN FIHANTGM*

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	178	189	RMTHIAVERNRR
2	238	242	KRRRL

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator (PubMed:17088607, PubMed:17183265, PubMed:17183267). Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity (PubMed:24571519). Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata (PubMed:17088607, PubMed:17183265, PubMed:17183267). Prevents histone H3K27me3 marks and derepresses stem cell gene expression (PubMed:24654956). {ECO:0000269\|PubMed:17088607, ECO:0000269\|PubMed:17183265, ECO:0000269\|PubMed:17183267, ECO:0000269\|PubMed:24571519, ECO:0000269\|PubMed:24654956}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Inhibited by low relative humidity (LRH) via epigenetic CG methylation, thus leading to a reduced stomatal index. {ECO:0000269\|PubMed:22442411}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	LN681848	0.0	LN681848.1 Cucumis melo genomic scaffold, anchoredscaffold00006.
GenBank	LN713260	0.0	LN713260.1 Cucumis melo genomic chromosome, chr_6.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_016898993.1	0.0	PREDICTED: transcription factor FAMA
Swissprot	Q56YJ8	1e-131	FAMA_ARATH; Transcription factor FAMA
TrEMBL	A0A1S4DSQ1	0.0	A0A1S4DSQ1_CUCME; transcription factor FAMA
STRING	XP_008438368.1	0.0	(Cucumis melo)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF10489	33	40

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G24140.1	1e-104	bHLH family protein

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Matos JL, et al. Irreversible fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RETINOBLASTOMA-RELATED module. Elife, 2015. [PMID:25303364] Chen L, et al. NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development, 2016. 143(9): p. 1600-11 [PMID:26989174] Lee JH,Jung JH,Park CM Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis. Plant Cell, 2017. 29(11): p. 2817-2830 [PMID:29070509] Han X, et al. Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons. Plant Physiol., 2018. 176(4): p. 2871-2885 [PMID:29496884] Li X,Yang R,Chen H The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA. PLoS ONE, 2018. 13(3): p. e0193458 [PMID:29513733]