PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Csa10g007600.1

Organism

Camelina sativa

Taxonomic ID

90675

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Camelina

Family

bHLH

Protein Properties

Length: 594aa MW: 66847.2 Da PI: 5.5888

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Csa10g007600.1	genome	CSGP	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	29.5	1.4e-09	181	228	4	55
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +h+++Er RR++i +++ L++l+P + +k Ka +L + ++Y++sLq Csa10g007600.1 181 SHSIAERVRREKISERMKFLQDLVPGC----DKITGKAGMLDEIINYVQSLQ 228 8***********************9....899***************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47459	2.75E-16	175	246	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	1.07E-10	175	232	No hit	No description
PROSITE profile	PS50888	15.593	177	227	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	8.0E-16	177	238	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	5.5E-7	181	228	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	2.0E-8	183	233	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF05368	1.4E-55	344	582	IPR008030	NmrA-like domain
SuperFamily	SSF51735	2.18E-33	344	564	IPR016040	NAD(P)-binding domain
Gene3D	G3DSA:3.90.25.10	2.2E-44	437	591	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 594 aa Download sequence Send to blast
MNGAIEADLL LDYNTDMSVL ERQRAHLKYL NPGFDSPLAG FFSDSSMING GVIDGFLGTD 60 GLNLPMIYGQ TTVEGDPRLS ISPETMIGTG NFKKRKFDTE TKDCDGKKKK ILMMNREDEV 120 EEEDEKSKLT EQNNGSTKSI KKMKNKAKKE ENSYSNDSSK VTIQKTDYIH VRARRGQATD 180 SHSIAERVRR EKISERMKFL QDLVPGCDKI TGKAGMLDEI INYVQSLQRQ IEFLSMKLAV 240 VNPRPDFDMD DIFAKEVAST PMTVVPSPEM VNSGYSHEMV NSGYSNEMVS SGYLHFNPMQ 300 QVDTTSSDPL SSCFNKLFDY VTEWPSSFDV GLSCAESLWK FRSLIKGSLE DERSLEEAVS 360 KVDVVISAIP SKHVLDQKLL IKVIKQAGSI KRFIPAEYGA NPDKTQVSDL DHGFYSKKSQ 420 IKRMIESEGI PYTYICCGLF MRILLPSLVQ PGLHSPPIDK ATVFGDGNVK AVFVNDVDVA 480 AFTIKTIDDP RTLNKTLYLR PPENICSMND LVHLWEGKIE KKLEKTFVTE NQLLKKIKET 540 PYPDNMEMVF IYSVFIKGDH TYFDIESSGG VNGTELYPDV KYMTVSEFLH TLR*

Sequence ? help Back to Top

Protein Sequence Length: 594 aa Download sequence Send to blast

MNGAIEADLL LDYNTDMSVL ERQRAHLKYL NPGFDSPLAG FFSDSSMING GVIDGFLGTD  60
GLNLPMIYGQ TTVEGDPRLS ISPETMIGTG NFKKRKFDTE TKDCDGKKKK ILMMNREDEV  120
EEEDEKSKLT EQNNGSTKSI KKMKNKAKKE ENSYSNDSSK VTIQKTDYIH VRARRGQATD  180
SHSIAERVRR EKISERMKFL QDLVPGCDKI TGKAGMLDEI INYVQSLQRQ IEFLSMKLAV  240
VNPRPDFDMD DIFAKEVAST PMTVVPSPEM VNSGYSHEMV NSGYSNEMVS SGYLHFNPMQ  300
QVDTTSSDPL SSCFNKLFDY VTEWPSSFDV GLSCAESLWK FRSLIKGSLE DERSLEEAVS  360
KVDVVISAIP SKHVLDQKLL IKVIKQAGSI KRFIPAEYGA NPDKTQVSDL DHGFYSKKSQ  420
IKRMIESEGI PYTYICCGLF MRILLPSLVQ PGLHSPPIDK ATVFGDGNVK AVFVNDVDVA  480
AFTIKTIDDP RTLNKTLYLR PPENICSMND LVHLWEGKIE KKLEKTFVTE NQLLKKIKET  540
PYPDNMEMVF IYSVFIKGDH TYFDIESSGG VNGTELYPDV KYMTVSEFLH TLR*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1qyc_A	5e-79	335	589	47	304	phenylcoumaran benzylic ether reductase PT1
1qyc_B	5e-79	335	589	47	304	phenylcoumaran benzylic ether reductase PT1
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	93	109	KRKFDTETKDCDGKKKK

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor that binds DNA to G box 5'-CACGTG-3' and, to a lower extent, to E-box 5'-CANNTG-3' in vitro. Binds to chromatin DNA of the FT gene and promotes its expression, and thus triggers flowering in response to blue light. {ECO:0000269\|PubMed:18988809, ECO:0000269\|PubMed:24130508}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Csa10g007600.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Accumulates strongly in response to blue light due to reduced preventing 26S proteasome-mediated degradation in an ADO1/ZTL and ADO2/LKP2 dependent manner, but levels decrease in the absence of blue light via 26S proteasome degradation (at protein level). {ECO:0000269\|PubMed:24101505}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK117846	0.0	AK117846.1 Arabidopsis thaliana At4g34530 mRNA for putative bHLH transcription factor (bHLH063), complete cds, clone: RAFL18-04-K08.
GenBank	AY120741	0.0	AY120741.1 Arabidopsis thaliana unknown protein (At4g34530:At4g34540) mRNA, complete cds.
GenBank	BT005313	0.0	BT005313.1 Arabidopsis thaliana At4g34530 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_010432403.1	0.0	PREDICTED: transcription factor bHLH63-like
Swissprot	Q8GY61	0.0	BH063_ARATH; Transcription factor bHLH63
TrEMBL	R0F5T5	0.0	R0F5T5_9BRAS; Uncharacterized protein
STRING	XP_010432403.1	0.0	(Camelina sativa)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM11509	2	2

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G34530.1	1e-177	cryptochrome-interacting basic-helix-loop-helix 1

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Yang D,Zhao W,Meng Y,Li H,Liu B A CIB1-LIKE transcription factor GmCIL10 from soybean positively regulates plant flowering. Sci China Life Sci, 2015. 58(3): p. 261-9 [PMID:25651969] Katsura Y, et al. An optogenetic system for interrogating the temporal dynamics of Akt. Sci Rep, 2015. 5: p. 14589 [PMID:26423353] Taslimi A, et al. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Nat. Chem. Biol., 2016. 12(6): p. 425-30 [PMID:27065233] Liu Q, et al. The Blue Light-Dependent Polyubiquitination and Degradation of Arabidopsis Cryptochrome2 Requires Multiple E3 Ubiquitin Ligases. Plant Cell Physiol., 2016. 57(10): p. 2175-2186 [PMID:27516416] Pathak GP, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res., 2017. 45(20): p. e167 [PMID:28431041] Duan L, et al. Understanding CRY2 interactions for optical control of intracellular signaling. Nat Commun, 2017. 8(1): p. 547 [PMID:28916751] Quejada JR, et al. Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1. Nucleic Acids Res., 2017. 45(20): p. e172 [PMID:29040770] Liu Y, et al. CIB1 and CO interact to mediate CRY2-dependent regulation of flowering. EMBO Rep., 2019. [PMID:30126927]