PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID GSBRNA2T00150198001
Common NameGSBRNA2T00150198001
Organism
Brassica napus
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica
Family bHLH
Protein Properties Length: 569aa    MW: 64604.9 Da    PI: 5.0845
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
GSBRNA2T00150198001genomeGenoscopeView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH39.31.2e-12316359755
                          HHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
                  HLH   7 erErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                           +ErrRR+++N+++  Lr+l+P       +Kl+ a+iL  A++Y+k+Lq
  GSBRNA2T00150198001 316 HAERRRRKKLNDRLYALRSLVPRI-----TKLDRASILGDAINYVKELQ 359
                          68*********************7.....7******************9 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF142151.6E-1610148IPR025610Transcription factor MYC/MYB N-terminal
CDDcd000832.12E-14308361No hitNo description
PROSITE profilePS5088816.247309358IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474592.36E-17311374IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003537.7E-16315364IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000103.2E-10316359IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.102.0E-16317372IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0046983Molecular Functionprotein dimerization activity
Sequence ? help Back to Top
Protein Sequence    Length: 569 aa     Download sequence    Send to blast
MESNMQNLLE KLRPLVGARA WDYCVLWSLN EDQRFVKWMG CCCGGTQLIE EAGTEEFSFG  60
GCRDVMFHHP RTKSCEFLAH LPSSISLDSG IYAETLLTNQ TGWLTESSGP GFMQETICTR  120
VLVPIPRGLV ELFAARHVAE DQNVVDFVMG HCNMLMDETV RINMMAGDKV ESKPYGMLLP  180
GDIYQKGSSK DEEMMNLPSP YDISADQIRL NFLPQMNDYE SQQHLKMKSD YDHHHQTLGY  240
DLPENGNNMN PFNTVAEEGM SVMGEASLLV NEQQVGNDKE MNENATGSDC SDQIDDEDDP  300
KRKKKTGKHT QAKNLHAERR RRKKLNDRLY ALRSLVPRIT KLDRASILGD AINYVKELQN  360
EAKELQDELE ENSETEDGSN RQQGGMSLNG TVLTGFHQGL SCNSNLPDMK QDVDLENCND  420
KGQEMEPQVD VAHLDGREFF VKVICEYKPG GFTRLMEALD SLGLEVTNAN TTRFLSLVSN  480
VFKVEKNDSE MVPAEHVRNS LLEITRNTSR GWHDDQMATG SMQNEKNEVD YQHYDDHHHH  540
GGHHQLHDHQ MNQSAHHHHQ HINHYQNQ*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5gnj_A2e-12307371273Transcription factor MYC2
5gnj_B2e-12307371273Transcription factor MYC2
5gnj_E2e-12307371273Transcription factor MYC2
5gnj_F2e-12307371273Transcription factor MYC2
5gnj_G2e-12307371273Transcription factor MYC2
5gnj_I2e-12307371273Transcription factor MYC2
5gnj_M2e-12307371273Transcription factor MYC2
5gnj_N2e-12307371273Transcription factor MYC2
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1302323KKKTGKHTQAKNLHAERRRRKK
2317324ERRRRKKL
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Bna.22630.0flower
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: First observed a bud stage of 0.6-mm, shortly after microspore release from the postmeiotic tetrads. Later restricted within the tapetum, microspores and anther locule. Still visible within the haploid nuclei, each of which had migrated to the pollen cell wall prior to pollen mitosis I. Not expressed later. {ECO:0000269|PubMed:12535353}.
UniprotTISSUE SPECIFICITY: Mostly expressed in closed, post-meiotic buds, and, to a lower extent, in pre-meiotic buds. Detected in leaves, stems, and flowers. {ECO:0000269|PubMed:12535353, ECO:0000269|PubMed:12679534}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther (PubMed:12535353). Binds E-box regions in the AHL16/TEK promoter. {ECO:0000269|PubMed:12535353, ECO:0000269|PubMed:24804694}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapGSBRNA2T00150198001
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAJ5119910.0AJ511991.1 Brassica napus mRNA for bHLH transcription factor.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_013629190.10.0PREDICTED: transcription factor ABORTED MICROSPORES-like
RefseqXP_013629191.10.0PREDICTED: transcription factor ABORTED MICROSPORES-like
SwissprotQ9ZVX20.0AMS_ARATH; Transcription factor ABORTED MICROSPORES
TrEMBLA0A3N6Q4810.0A0A3N6Q481_BRACR; Uncharacterized protein
STRINGBo3g091780.10.0(Brassica oleracea)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM75892741
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT2G16910.10.0bHLH family protein
Publications ? help Back to Top
  1. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  2. Jiang J,Zhang Z,Cao J
    Pollen wall development: the associated enzymes and metabolic pathways.
    Plant Biol (Stuttg), 2013. 15(2): p. 249-63
    [PMID:23252839]
  3. Xu J, et al.
    ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis.
    Plant Cell, 2014. 26(4): p. 1544-1556
    [PMID:24781116]
  4. Chalhoub B, et al.
    Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome.
    Science, 2014. 345(6199): p. 950-3
    [PMID:25146293]
  5. Qian H, et al.
    Trace concentrations of imazethapyr (IM) affect floral organs development and reproduction in Arabidopsis thaliana: IM-induced inhibition of key genes regulating anther and pollen biosynthesis.
    Ecotoxicology, 2015. 24(1): p. 163-71
    [PMID:25348600]
  6. Xiong SX, et al.
    The transcription factors MS188 and AMS form a complex to activate the expression of CYP703A2 for sporopollenin biosynthesis in Arabidopsis thaliana.
    Plant J., 2016. 88(6): p. 936-946
    [PMID:27460657]
  7. Ferguson AC, et al.
    Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis.
    New Phytol., 2017. 213(2): p. 778-790
    [PMID:27787905]
  8. Lou Y, et al.
    Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for anther development in Arabidopsis thaliana.
    New Phytol., 2018. 217(1): p. 378-391
    [PMID:28940573]
  9. Bi H,Fan W,Zhang P
    C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1689
    [PMID:29021807]