PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

GSBRNA2T00126445001

Common Name

BnAP3, GSBRNA2T00126445001, LOC106449291

Organism

Brassica napus

Taxonomic ID

3708

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Family

MIKC_MADS

Protein Properties

Length: 225aa MW: 26378.8 Da PI: 8.6586

Description

MIKC_MADS family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
GSBRNA2T00126445001	genome	Genoscope	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	SRF-TF	93.8	7.7e-30	9	59	1	51
S---SHHHHHHHHHHHHHHHHHHHHHHHHHHT-EEEEEEE-TTSEEEEEE- CS SRF-TF 1 krienksnrqvtfskRrngilKKAeELSvLCdaevaviifsstgklyeyss 51 krien++nrqvt+skRrng++KKA+EL vLCda+v++i+fss++kl+e++s GSBRNA2T00126445001 9 KRIENQTNRQVTYSKRRNGLFKKAHELTVLCDARVSIIMFSSSNKLHEFIS 59 79***********************************************86 PP
2	K-box	80.5	4.1e-27	71	169	1	99
K-box 1 yqkssgksleeakaeslqqelakLkkeienLqreqRhllGedLesLslkeLqqLeqqLekslkkiRskKnellleqieelqkkekelqee 90 yq+ s++++++a++e++q+ +kL + ++nL+++++++lGe+L++L+++eL++Le+++e+ k +R++K + l +qie+++kk+k++q++ GSBRNA2T00126445001 71 YQTVSDVDVWSAHYERMQETKRKLLETNRNLRTQIKQRLGECLDELDIQELRSLEEEMENTFKLVRERKFKSLGNQIETTKKKNKSQQDI 160 78899999******************************************************************************** PP K-box 91 nkaLrkkle 99 +k+L ++le GSBRNA2T00126445001 161 QKNLIHELE 169 **99875 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SMART	SM00432	9.7E-41	1	60	IPR002100	Transcription factor, MADS-box
PROSITE profile	PS50066	32.226	1	61	IPR002100	Transcription factor, MADS-box
CDD	cd00265	2.21E-40	2	80	No hit	No description
PROSITE pattern	PS00350	0	3	57	IPR002100	Transcription factor, MADS-box
PRINTS	PR00404	7.4E-29	3	23	IPR002100	Transcription factor, MADS-box
SuperFamily	SSF55455	3.01E-36	3	94	IPR002100	Transcription factor, MADS-box
Pfam	PF00319	1.8E-25	10	57	IPR002100	Transcription factor, MADS-box
PRINTS	PR00404	7.4E-29	23	38	IPR002100	Transcription factor, MADS-box
PRINTS	PR00404	7.4E-29	38	59	IPR002100	Transcription factor, MADS-box
Pfam	PF01486	6.5E-16	82	168	IPR002487	Transcription factor, K-box
PROSITE profile	PS51297	13.906	84	174	IPR002487	Transcription factor, K-box

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 225 aa Download sequence Send to blast
MARGKIQIKR IENQTNRQVT YSKRRNGLFK KAHELTVLCD ARVSIIMFSS SNKLHEFISP 60 NTTTKEIIDL YQTVSDVDVW SAHYERMQET KRKLLETNRN LRTQIKQRLG ECLDELDIQE 120 LRSLEEEMEN TFKLVRERKF KSLGNQIETT KKKNKSQQDI QKNLIHELEL RAEDPHYGLV 180 DNGGDYDSVL GYQLRFHQNH HHHYPNHALH AASASDIITF HLLE*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
6byy_A	2e-17	1	61	1	61	MEF2 CHIMERA
6byy_B	2e-17	1	61	1	61	MEF2 CHIMERA
6byy_C	2e-17	1	61	1	61	MEF2 CHIMERA
6byy_D	2e-17	1	61	1	61	MEF2 CHIMERA
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Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Bna.7355	1e-178	flower

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in petals and stamens.

Functional Description ? help Back to Top
Source	Description
UniProt	Probable transcription factor involved in the genetic control of flower development. Is required for normal development of petals and stamens in the wild-type flower. Forms a heterodimer with PISTILLATA that is required for autoregulation of both AP3 and PI genes. AP3/PI heterodimer interacts with APETALA1 or SEPALLATA3 to form a ternary complex that could be responsible for the regulation of the genes involved in the flower development. AP3/PI heterodimer activates the expression of NAP. AP3/PI prevents GATA22/GNL and GATA21/GNC expression (PubMed:18417639). {ECO:0000269\|PubMed:18417639, ECO:0000269\|PubMed:8565821, ECO:0000269\|PubMed:9489703}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	GSBRNA2T00126445001

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Positively regulated by the meristem identity proteins APETALA1 and LEAFY with the cooperation of UFO. Repressed by silencing mediated by polycomb group (PcG) protein complex containing EMF1 and EMF2. {ECO:0000269\|PubMed:11283333, ECO:0000269\|PubMed:19783648}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF124814	0.0	AF124814.1 Brassica napus APETALA3 (BnAP3) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001302937.1	1e-167	floral homeotic protein APETALA 3-like
Swissprot	P35632	1e-158	AP3_ARATH; Floral homeotic protein APETALA 3
TrEMBL	A0A397ZQB5	1e-165	A0A397ZQB5_BRACM; Uncharacterized protein
TrEMBL	Q6IV03	1e-165	Q6IV03_BRARC; Floral homeotic protein APETALA3
TrEMBL	Q9M7M0	1e-165	Q9M7M0_BRANA; APETALA3-4
STRING	Bra014822.1-P	1e-166	(Brassica rapa)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM5261	27	50

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G54340.1	1e-145	MIKC_MADS family protein

Link Out ? help Back to Top
Entrez Gene	106449291

Publications ? help Back to Top
Pylatuik JD,Lindsay DL,Davis AR,Bonham-Smith PC Isolation and characterization of a Brassica napus cDNA corresponding to a B-class floral development gene. J. Exp. Bot., 2003. 54(391): p. 2385-7 [PMID:12909692] Furner I,Ellis L,Bakht S,Mirza B,Sheikh M CAUT lines: a novel resource for studies of cell autonomy in Arabidopsis. Plant J., 2008. 53(4): p. 645-60 [PMID:18269574] Jing D, et al. Two ancestral APETALA3 homologs from the basal angiosperm Magnolia wufengensis (Magnoliaceae) can affect flower development of Arabidopsis. Gene, 2014. 537(1): p. 100-7 [PMID:24334124] Zhang Y, et al. A cucumber DELLA homolog CsGAIP may inhibit staminate development through transcriptional repression of B class floral homeotic genes. PLoS ONE, 2014. 9(3): p. e91804 [PMID:24632777] Nakamura Y,Liu YC,Lin YC Floral glycerolipid profiles in homeotic mutants of Arabidopsis thaliana. Biochem. Biophys. Res. Commun., 2014. 450(4): p. 1272-5 [PMID:24984150] Chalhoub B, et al. Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome. Science, 2014. 345(6199): p. 950-3 [PMID:25146293] Behrend A,Borchert T,Hohe A "The usual suspects"- analysis of transcriptome sequences reveals deviating B gene activity in C. vulgaris bud bloomers. BMC Plant Biol., 2015. 15: p. 8 [PMID:25604890] Chen Z, et al. Overexpression of AtAP1M3 regulates flowering time and floral development in Arabidopsis and effects key flowering-related genes in poplar. Transgenic Res., 2015. 24(4): p. 705-15 [PMID:25820621] Yunus IS, et al. Phosphatidic acid is a major phospholipid class in reproductive organs of Arabidopsis thaliana. Plant Signal Behav, 2015. 10(8): p. e1049790 [PMID:26179579] Sun JJ, et al. CsAP3: A Cucumber Homolog to Arabidopsis APETALA3 with Novel Characteristics. Front Plant Sci, 2016. 7: p. 1181 [PMID:27540391]