PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

GSBRNA2T00107889001

Common Name

GSBRNA2T00107889001

Organism

Brassica napus

Taxonomic ID

3708

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Family

bHLH

Protein Properties

Length: 135aa MW: 16003.6 Da PI: 11.4327

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
GSBRNA2T00107889001	genome	Genoscope	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	28.1	3.8e-09	99	134	4	44
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiL 44 +h + rrRR+++N++f +Lr+l+P +K++K++iL GSBRNA2T00107889001 99 NHVVAMRRRREKLNERFITLRSLVPFV-----TKMDKVSIL 134 688999******************8.....7****98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50888	13.681	95	134	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	5.37E-10	96	134	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	4.54E-8	98	134	No hit	No description
Gene3D	G3DSA:4.10.280.10	4.7E-9	99	134	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	8.3E-6	100	134	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 135 aa Download sequence Send to blast
MTTSRHKPSV YPSPSQHILH PYGSTCIFTT HRSSFRFSFH IFLPFISWRV ENVKEHQQYQ 60 RVEKAASSSS QWMLKHIILK VPFVHDNTKN KRLSREELNH VVAMRRRREK LNERFITLRS 120 LVPFVTKMDK VSIL*

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	104	109	RRRREK

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Bna.6320	1e-132	seed

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Faint expression in buds and flowers. Increases during the very early stages of seed development, reaches a maximum at the globular embryo stage and stays high throughout seed formation.
Uniprot	TISSUE SPECIFICITY: Buds, flowers and developing siliques, but not in leaves, stems and roots. {ECO:0000269\|PubMed:12679534}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator, when associated with MYB75/PAP1 or MYB90/PAP2. Involved in the control of flavonoid pigmentation. Plays a key role in regulating leucoanthocyanidin reductase (BANYULS) and dihydroflavonol-4-reductase (DFR). Not required for leucoanthocyanidin dioxygenase (LDOX) expression. {ECO:0000269\|PubMed:15361138}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	GSBRNA2T00107889001

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	GU219990	1e-139	GU219990.1 Brassica oleracea var. botrytis cultivar Stovepipe TT8 (TT8) gene, complete cds.
GenBank	GU255865	1e-139	GU255865.1 Brassica napus cultivar Darmor-bzh bHLH DNA binding domain transcription factor BnaC.TT8 (TT8) gene, complete cds.
GenBank	GU255867	1e-139	GU255867.1 Brassica oleracea cultivar C10 bHLH DNA binding domain transcription factor BolC.TT8 (TT8) gene, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_013609428.1	2e-52	PREDICTED: transcription factor TT8
Swissprot	Q9FT81	1e-36	TT8_ARATH; Transcription factor TT8
TrEMBL	A0A0D3E8T7	1e-91	A0A0D3E8T7_BRAOL; Uncharacterized protein
TrEMBL	A0A3P6EFG3	2e-93	A0A3P6EFG3_BRAOL; Uncharacterized protein
STRING	Bo9g086920.1	2e-92	(Brassica oleracea)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM9422	28	36

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G09820.1	9e-39	bHLH family protein

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Kim K,Jiang K,Teng SL,Feldman LJ,Huang H Using biologically interrelated experiments to identify pathway genes in Arabidopsis. Bioinformatics, 2012. 28(6): p. 815-22 [PMID:22271267] Liu Z,Shi MZ,Xie DY Regulation of anthocyanin biosynthesis in Arabidopsis thaliana red pap1-D cells metabolically programmed by auxins. Planta, 2014. 239(4): p. 765-81 [PMID:24370633] Chalhoub B, et al. Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome. Science, 2014. 345(6199): p. 950-3 [PMID:25146293] Ranocha P,Francoz E,Burlat V,Dunand C Expression of PRX36, PMEI6 and SBT1.7 is controlled by complex transcription factor regulatory networks for proper seed coat mucilage extrusion. Plant Signal Behav, 2014. 9(11): p. e977734 [PMID:25531128] Zhu Z, et al. Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation. J. Exp. Bot., 2015. 66(13): p. 3775-89 [PMID:25911741] Zhu Q, et al. Ectopic Expression of the Coleus R2R3 MYB-Type Proanthocyanidin Regulator Gene SsMYB3 Alters the Flower Color in Transgenic Tobacco. PLoS ONE, 2015. 10(10): p. e0139392 [PMID:26448466] Li P, et al. Regulation of anthocyanin and proanthocyanidin biosynthesis by Medicago truncatula bHLH transcription factor MtTT8. New Phytol., 2016. 210(3): p. 905-21 [PMID:26725247] Bulgakov VP,Veremeichik GN,Grigorchuk VP,Rybin VG,Shkryl YN The rolB gene activates secondary metabolism in Arabidopsis calli via selective activation of genes encoding MYB and bHLH transcription factors. Plant Physiol. Biochem., 2016. 102: p. 70-9 [PMID:26913794] Petridis A,Döll S,Nichelmann L,Bilger W,Mock HP Arabidopsis thaliana G2-LIKE FLAVONOID REGULATOR and BRASSINOSTEROID ENHANCED EXPRESSION1 are low-temperature regulators of flavonoid accumulation. New Phytol., 2016. 211(3): p. 912-25 [PMID:27125220] Rai A, et al. Coordinate Regulation of Metabolite Glycosylation and Stress Hormone Biosynthesis by TT8 in Arabidopsis. Plant Physiol., 2016. 171(4): p. 2499-515 [PMID:27432888] Li Y, et al. Two IIIf Clade-bHLHs from Freesia hybrida Play Divergent Roles in Flavonoid Biosynthesis and Trichome Formation when Ectopically Expressed in Arabidopsis. Sci Rep, 2016. 6: p. 30514 [PMID:27465838] Mahmood K,Xu Z,El-Kereamy A,Casaretto JA,Rothstein SJ The Arabidopsis Transcription Factor ANAC032 Represses Anthocyanin Biosynthesis in Response to High Sucrose and Oxidative and Abiotic Stresses. Front Plant Sci, 2016. 7: p. 1548 [PMID:27790239] Zou B, et al. Calmodulin-binding protein CBP60g functions as a negative regulator in Arabidopsis anthocyanin accumulation. PLoS ONE, 2017. 12(3): p. e0173129 [PMID:28253311] Chen K,Liu H,Lou Q,Liu Y Ectopic Expression of the Grape Hyacinth (Muscari armeniacum) R2R3-MYB Transcription Factor Gene, MaAN2, Induces Anthocyanin Accumulation in Tobacco. Front Plant Sci, 2017. 8: p. 965 [PMID:28642775] Kataya ARA, et al. PLATINUM SENSITIVE 2 LIKE impacts growth, root morphology, seed set, and stress responses. PLoS ONE, 2017. 12(7): p. e0180478 [PMID:28678890] Ezer D, et al. The G-Box Transcriptional Regulatory Code in Arabidopsis. Plant Physiol., 2017. 175(2): p. 628-640 [PMID:28864470] Feng K, et al. An R2R3-MYB transcription factor, OjMYB1, functions in anthocyanin biosynthesis in Oenanthe javanica. Planta, 2018. 247(2): p. 301-315 [PMID:28965159] Wen J, et al. The C-terminal domains of Arabidopsis GL3/EGL3/TT8 interact with JAZ proteins and mediate dimeric interactions. Plant Signal Behav, 2018. 13(1): p. e1422460 [PMID:29293407]