PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID GSBRNA2T00094727001
Common NameGSBRNA2T00094727001
Organism
Brassica napus
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica
Family MIKC_MADS
Protein Properties Length: 319aa    MW: 37327.2 Da    PI: 9.6547
Description MIKC_MADS family protein
Gene Model
Gene Model ID Type Source Coding Sequence
GSBRNA2T00094727001genomeGenoscopeView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1SRF-TF78.45e-2583133151
                          S---SHHHHHHHHHHHHHHHHHHHHHHHHHHT-EEEEEEE-TTSEEEEEE- CS
               SRF-TF   1 krienksnrqvtfskRrngilKKAeELSvLCdaevaviifsstgklyeyss 51 
                          k+ienk+ rqvtfskRrng++KK  ELSvLCda + +i+fs+tgkl  y+s
  GSBRNA2T00094727001  83 KKIENKTSRQVTFSKRRNGLIKKTRELSVLCDAHIGLIVFSTTGKLTQYCS 133
                          68***********************************************96 PP
2K-box56.41.3e-191612411595
                K-box  15 eslqqelakLkkeienLqreqRhllGedLesLslkeLqqLeqqLekslkkiRskKnellleqieelqkkekelqeenkaLr 95 
                            l++e++ L++e+ +L+  +R + G dL s+   eL+ LeqqLe+s++k+R++K+ell +q+ +l +k+++l+++n+++ 
  GSBRNA2T00094727001 161 DDLRHEIEILRRETCKLELRLRPYHGHDLASIPPHELEGLEQQLEHSVRKVRERKKELLQQQLGNLSRKKRMLEDDNNNMY 241
                          56889************************************************************************9976 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE profilePS5006630.44575135IPR002100Transcription factor, MADS-box
SMARTSM004322.1E-3875134IPR002100Transcription factor, MADS-box
CDDcd002655.58E-4076153No hitNo description
SuperFamilySSF554553.27E-3076159IPR002100Transcription factor, MADS-box
PROSITE patternPS00350077131IPR002100Transcription factor, MADS-box
PRINTSPR004042.9E-277797IPR002100Transcription factor, MADS-box
PfamPF003194.5E-2484131IPR002100Transcription factor, MADS-box
PRINTSPR004042.9E-2797112IPR002100Transcription factor, MADS-box
PRINTSPR004042.9E-27112133IPR002100Transcription factor, MADS-box
PfamPF014861.9E-18159244IPR002487Transcription factor, K-box
PROSITE profilePS5129713.142160250IPR002487Transcription factor, K-box
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Sequence ? help Back to Top
Protein Sequence    Length: 319 aa     Download sequence    Send to blast
MNSLQGYYGL KPTNRRASKE SRSLVDAVIS GFNQSSKPSK EVRCDKPMRD ANNLKLDFSL  60
RENRNSSSRR KRKEMGRGKI EIKKIENKTS RQVTFSKRRN GLIKKTRELS VLCDAHIGLI  120
VFSTTGKLTQ YCSEHSNMPQ LIDRYLTKEG LQLPDLNDDR DDLRHEIEIL RRETCKLELR  180
LRPYHGHDLA SIPPHELEGL EQQLEHSVRK VRERKKELLQ QQLGNLSRKK RMLEDDNNNM  240
YRWLHDEHRT GVEFQQAGIE TKPMEYQQFL EQVQYYNDHH QQQSSVLQLP TLPSEIDLSY  300
HLQLAQPNLQ NDPTTKVD*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5f28_A1e-1675145169MEF2C
5f28_B1e-1675145169MEF2C
5f28_C1e-1675145169MEF2C
5f28_D1e-1675145169MEF2C
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
16873RRKRKE
2208216RKVRERKKE
3213230RKKELLQQQLGNLSRKKR
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Bna.133050.0seed
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Expressed during seed development.
UniprotTISSUE SPECIFICITY: Expressed in buds, flowers and immature seeds, but not in roots, stems, leaves, seedlings or siliques valves. Expression in seed coat is confined to the endothelium layer.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor involved in the developmental regulation of the endothelium and in the accumulation of proanthocyanidins (PAs) or condensed tannins which give the seed its brown pigmentation after oxidation (PubMed:12368498, PubMed:16080001). Necessary for the normal activation of the BANYULS promoter in the endothelium body (PubMed:12368498). Is required, together with AGL11/STK for the maternal control of endothelium formation, which is essential for female gametophyte development and fertilization, and seed formation (PubMed:22176531). Interacts genetically with AGL1/SHP1 and AGL5/SHP2 in a partially antagonistic manner and represses AGL1/SHP1, AGL5/SHP2, and AGL8/FUL during flower development. Is essential for the coordination of cell divisions in ovule, seed coat development and endosperm formation (PubMed:27776173). Mediates the crosstalk between endothelium and nucellus to ensure proper seed formation. Functions redundantly with AGL63/GOA to repress nucellus growth and promote its degeneration. Represses the negative regulator of autophagy and programmed cell death HVA22D in the proximal nucellus (PubMed:27233529). Binds specifically to the CArG box DNA sequence 5'-CC (A/T)6 GG-3' (PubMed:16080001). {ECO:0000269|PubMed:12368498, ECO:0000269|PubMed:16080001, ECO:0000269|PubMed:22176531, ECO:0000269|PubMed:27233529, ECO:0000269|PubMed:27776173}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapGSBRNA2T00094727001
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankHM4499890.0HM449989.1 Brassica napus cultivar DH12075 MADS-box DNA-binding domain transcription factor (TT16.4) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_009141444.10.0PREDICTED: protein TRANSPARENT TESTA 16-like isoform X1
RefseqXP_009141450.10.0PREDICTED: protein TRANSPARENT TESTA 16-like isoform X1
RefseqXP_013661058.10.0protein TRANSPARENT TESTA 16 isoform X1
SwissprotQ8RYD91e-130TT16_ARATH; Protein TRANSPARENT TESTA 16
TrEMBLA0A078FV430.0A0A078FV43_BRANA; BnaA09g05410D protein
TrEMBLA0A3P5YCZ00.0A0A3P5YCZ0_BRACM; Uncharacterized protein
STRINGBra026507.1-P0.0(Brassica rapa)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM65062543
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G23260.21e-118MIKC_MADS family protein
Publications ? help Back to Top
  1. Kim K,Jiang K,Teng SL,Feldman LJ,Huang H
    Using biologically interrelated experiments to identify pathway genes in Arabidopsis.
    Bioinformatics, 2012. 28(6): p. 815-22
    [PMID:22271267]
  2. Chalhoub B, et al.
    Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome.
    Science, 2014. 345(6199): p. 950-3
    [PMID:25146293]
  3. Rhodes DH, et al.
    Genome-wide association study of grain polyphenol concentrations in global sorghum [Sorghum bicolor (L.) Moench] germplasm.
    J. Agric. Food Chem., 2014. 62(45): p. 10916-27
    [PMID:25272193]
  4. Xu W, et al.
    Endosperm and Nucellus Develop Antagonistically in Arabidopsis Seeds.
    Plant Cell, 2016. 28(6): p. 1343-60
    [PMID:27233529]
  5. Ehlers K, et al.
    The MADS Box Genes ABS, SHP1, and SHP2 Are Essential for the Coordination of Cell Divisions in Ovule and Seed Coat Development and for Endosperm Formation in Arabidopsis thaliana.
    PLoS ONE, 2016. 11(10): p. e0165075
    [PMID:27776173]
  6. Coen O, et al.
    Developmental patterning of the sub-epidermal integument cell layer in Arabidopsis seeds.
    Development, 2017. 144(8): p. 1490-1497
    [PMID:28348169]
  7. Xu W, et al.
    TRANSPARENT TESTA 16 and 15 act through different mechanisms to control proanthocyanidin accumulation in Arabidopsis testa.
    J. Exp. Bot., 2017. 68(11): p. 2859-2870
    [PMID:28830101]
  8. Fiume E,Coen O,Xu W,Lepiniec L,Magnani E
    Developmental patterning of sub-epidermal cells in the outer integument of Arabidopsis seeds.
    PLoS ONE, 2017. 12(11): p. e0188148
    [PMID:29141031]