PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID GSBRNA2T00070223001
Common NameGSBRNA2T00070223001
Organism
Brassica napus
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica
Family WRKY
Protein Properties Length: 711aa    MW: 80355.1 Da    PI: 6.5208
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
GSBRNA2T00070223001genomeGenoscopeView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY96.12.4e-30200259159
                          ---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS
                 WRKY   1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 
                          ++Dg++WrKYGqK+ kg+++pr+YYrCt a gCpv+k+v+r+ ed +++++tYeg+Hnh+
  GSBRNA2T00070223001 200 MNDGCQWRKYGQKTAKGNPCPRAYYRCTVApGCPVRKQVQRCLEDMSILITTYEGTHNHP 259
                          58***************************99****************************7 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.20.25.803.0E-32185261IPR003657WRKY domain
SuperFamilySSF1182901.7E-27193261IPR003657WRKY domain
PROSITE profilePS5081128.828195261IPR003657WRKY domain
SMARTSM007741.9E-37200260IPR003657WRKY domain
PfamPF031063.4E-27201259IPR003657WRKY domain
PROSITE profilePS5173330.443442632IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain
SuperFamilySSF556818.29E-47443647No hitNo description
Gene3DG3DSA:3.30.930.101.0E-47445647No hitNo description
TIGRFAMsTIGR001212.3E-33460681IPR004408Biotin--acetyl-CoA-carboxylase ligase
PfamPF030998.4E-33460587IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain
PfamPF022372.5E-11649704IPR003142Biotin protein ligase, C-terminal
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0006464Biological Processcellular protein modification process
GO:0042966Biological Processbiotin carboxyl carrier protein biosynthetic process
GO:0005829Cellular Componentcytosol
GO:0009507Cellular Componentchloroplast
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0004077Molecular Functionbiotin-[acetyl-CoA-carboxylase] ligase activity
GO:0004078Molecular Functionbiotin-[methylcrotonoyl-CoA-carboxylase] ligase activity
GO:0043565Molecular Functionsequence-specific DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 711 aa     Download sequence    Send to blast
MGMDLSLKLE KEKDEERSTH ICERKELEEK EDASEDKDEK MVKEDEIEDS CLSSRTRQEE  60
KEREEIEMEH VKEENTRLRK LVEQTLQDYR QLEMKFPVID QTNNMYLETF LGAQAKDRKR  120
GVERYDSLNL VEGELGLSLS LQKKQKQDEN KELESNNTQR YNSSNKAQGQ DMNTQRVIMS  180
SPGNRKTRVS VRARCDTPTM NDGCQWRKYG QKTAKGNPCP RAYYRCTVAP GCPVRKQVQR  240
CLEDMSILIT TYEGTHNHPL PVGATAMAST ASASPFLLLD SSDNLSHPSY LQQNGSNKQS  300
VRSLINFDDS SFRGASRLRD FRASSAIIAT SVTPSSSSIL VVGLGMSCLP LSTYMQVANL  360
WRFVSLRPSW RLSFSVAATA MDGDLDSCSL VLCGKSSVEN DTAKRLKSEN VLKLPDVTTK  420
VSLFLDSEIK NLVRDDDSFN PSLFMNSLST ARFGRFLIWS PRLSSTHDVV SYNFSELPVG  480
SVCVSDIQFK GRGRTKNVWE SPKGCLMYSF TVEMEDGRIV PLIQYVVSLA VTEAVKDVCD  540
NKGLPYIDVK IKWPNDLYLN GLKVGGILCT STYRSRIFHV SVGVGLNVDN DQPTTCLNAV  600
LKDISPASVL LKREEIIAAF FHKFEKFFNL FIDQGFKSLE ELYYRTWLHS GQRVIVEDKI  660
EDQVVQNVVT IQGLTSSGYL LAIGDDYQMY ELHPDGNSFD FFKGLVRRKI *
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2ayd_A3e-22187263176WRKY transcription factor 1
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Bna.281220.0microspore| microspore-derived embryo
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in roots, leaves, stems, flowers, siliques and seeds. {ECO:0000269|PubMed:11784724, ECO:0000269|PubMed:18156294}.
Functional Description ? help Back to Top
Source Description
UniProtPlays a major role in biotin-dependent carboxylase biotinylation. Catalyzes the addition of biotin to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase and can also biotinylate methylcrotonyl-CoA carboxylase. Is responsible for most, if not all, biotin--protein ligase activity in Arabidopsis. Is essential for plant viability and required for ovule development. {ECO:0000269|PubMed:18156294, ECO:0000269|PubMed:9173880, ECO:0000269|PubMed:9874227}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapGSBRNA2T00070223001
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankKF4300320.0KF430032.1 Brassica rapa WRKY transcription factor 9 (WRKY9) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_013688682.10.0biotin--protein ligase 2-like
SwissprotQ9SL920.0HCS1_ARATH; Biotin--protein ligase 1, chloroplastic
TrEMBLA0A078FE950.0A0A078FE95_BRANA; BnaA02g13630D protein
STRINGBra033956.1-P0.0(Brassica rapa)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT1G68150.14e-96WRKY DNA-binding protein 9
Publications ? help Back to Top
  1. Denis L,Grossemy M,Douce R,Alban C
    Molecular characterization of a second copy of holocarboxylase synthetase gene (hcs2) in Arabidopsis thaliana.
    J. Biol. Chem., 2002. 277(12): p. 10435-44
    [PMID:11784724]
  2. Thelen JJ,Ohlrogge JB
    Both antisense and sense expression of biotin carboxyl carrier protein isoform 2 inactivates the plastid acetyl-coenzyme A carboxylase in Arabidopsis thaliana.
    Plant J., 2002. 32(4): p. 419-31
    [PMID:12445115]
  3. Puyaubert J,Denis L,Alban C
    Dual targeting of Arabidopsis holocarboxylase synthetase1: a small upstream open reading frame regulates translation initiation and protein targeting.
    Plant Physiol., 2008. 146(2): p. 478-91
    [PMID:18156294]
  4. Waterworth WM, et al.
    DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks.
    BMC Plant Biol., 2009. 9: p. 79
    [PMID:19558640]
  5. Chen X,Chou HH,Wurtele ES
    Holocarboxylase synthetase 1 physically interacts with histone h3 in Arabidopsis.
    Scientifica (Cairo), 2013. 2013: p. 983501
    [PMID:24278788]
  6. Chalhoub B, et al.
    Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome.
    Science, 2014. 345(6199): p. 950-3
    [PMID:25146293]
  7. Tissot G,Douce R,Alban C
    Evidence for multiple forms of biotin holocarboxylase synthetase in pea (Pisum sativum) and in Arabidopsis thaliana: subcellular fractionation studies and isolation of a cDNA clone.
    Biochem. J., 1997. 323 ( Pt 1): p. 179-88
    [PMID:9173880]
  8. Tissot G,Pepin R,Job D,Douce R,Alban C
    Purification and properties of the chloroplastic form of biotin holocarboxylase synthetase from Arabidopsis thaliana overexpressed in Escherichia coli.
    Eur. J. Biochem., 1998. 258(2): p. 586-96
    [PMID:9874227]