PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

GSBRNA2T00023683001

Common Name

GSBRNA2T00023683001

Organism

Brassica napus

Taxonomic ID

3708

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Family

bHLH

Protein Properties

Length: 450aa MW: 49641.5 Da PI: 5.3985

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
GSBRNA2T00023683001	genome	Genoscope	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	40	7.3e-13	273	318	4	54
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 +h e+Er+RR+++N++f Lr ++P+ K++Ka+ L A+ YI++L GSBRNA2T00023683001 273 NHVEAERQRREKLNQRFYSLRAVVPNV-----SKMDKASLLGDAISYINEL 318 799*********************6.....5*************998 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	7.7E-48	50	213	IPR025610	Transcription factor MYC/MYB N-terminal
PROSITE profile	PS50888	17.282	269	318	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	7.59E-19	272	338	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	5.09E-15	272	323	No hit	No description
Pfam	PF00010	2.7E-10	273	318	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	1.4E-18	273	337	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	9.8E-17	275	324	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 450 aa Download sequence Send to blast
MNDYFLNEST PPGDNASTAM EAFIGTNHSS LWPQPPVPTP SLSQFNEDTL QQRLQALIES 60 AGEKWTYAIF WQISHDFESP AGDNAVVLGW GDGYYKGEED KEKKKKSSNS NPAEQEHRKR 120 EVTDTEWFFL VSMTQSFANG VGLPGESFLN SRVIWLSGSG ALTGSGCERA NQGQIYGLQT 180 MVCIAAENGV VELGSSEAIS QSSDLMDKVN SLFNSSNGML SFSTVVRSTA KSGESDHSDL 240 EASVVKEAIV VEPEKKPRKR GRKPANGREE PLNHVEAERQ RREKLNQRFY SLRAVVPNVS 300 KMDKASLLGD AISYINELKS KLQQAESEKE EIQKQLDGMS KEGNGKSGAS RAVKERRSSY 360 QDSASSVEME IDVKIIGWDV MIRVQCSKKN HPGSRFMDAL KELDLEVNHA SLSVVNDLMI 420 QQATVKMGSQ FFNHDQLRAA LMLKVGGDN*

Sequence ? help Back to Top

Protein Sequence Length: 450 aa Download sequence Send to blast

MNDYFLNEST PPGDNASTAM EAFIGTNHSS LWPQPPVPTP SLSQFNEDTL QQRLQALIES  60
AGEKWTYAIF WQISHDFESP AGDNAVVLGW GDGYYKGEED KEKKKKSSNS NPAEQEHRKR  120
EVTDTEWFFL VSMTQSFANG VGLPGESFLN SRVIWLSGSG ALTGSGCERA NQGQIYGLQT  180
MVCIAAENGV VELGSSEAIS QSSDLMDKVN SLFNSSNGML SFSTVVRSTA KSGESDHSDL  240
EASVVKEAIV VEPEKKPRKR GRKPANGREE PLNHVEAERQ RREKLNQRFY SLRAVVPNVS  300
KMDKASLLGD AISYINELKS KLQQAESEKE EIQKQLDGMS KEGNGKSGAS RAVKERRSSY  360
QDSASSVEME IDVKIIGWDV MIRVQCSKKN HPGSRFMDAL KELDLEVNHA SLSVVNDLMI  420
QQATVKMGSQ FFNHDQLRAA LMLKVGGDN*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4rru_A	4e-99	6	209	4	227	Transcription factor MYC3
4ywc_A	4e-99	6	209	4	227	Transcription factor MYC3
4ywc_B	4e-99	6	209	4	227	Transcription factor MYC3
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	254	262	KKPRKRGRK

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Bna.6979	0.0	flower\| leaf\| seed

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Constitutively expressed in roots, stems, leaves, flowers, and seedlings. {ECO:0000269\|PubMed:12136026, ECO:0000269\|PubMed:12679534, ECO:0000269\|PubMed:21335373}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in tryptophan, jasmonic acid (JA) and other stress-responsive gene regulation. With MYC2 and MYC4, controls additively subsets of JA-dependent responses. Can form complexes with all known glucosinolate-related MYBs to regulate glucosinolate biosynthesis. Binds to the G-box (5'-CACGTG-3') of promoters. Activates multiple TIFY/JAZ promoters. {ECO:0000269\|PubMed:12136026, ECO:0000269\|PubMed:21242320, ECO:0000269\|PubMed:21321051, ECO:0000269\|PubMed:21335373, ECO:0000269\|PubMed:23943862}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	GSBRNA2T00023683001

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Barely up-regulated by jasmonic acid. {ECO:0000269\|PubMed:21242320, ECO:0000269\|PubMed:21335373}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK352707	0.0	AK352707.1 Thellungiella halophila mRNA, complete cds, clone: RTFL01-05-E20.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_009101493.1	1e-160	PREDICTED: transcription factor MYC3
Refseq	XP_013672555.1	1e-160	transcription factor MYC3-like isoform X4
Refseq	XP_022572553.1	1e-160	transcription factor MYC3-like isoform X2
Refseq	XP_022572554.1	1e-160	transcription factor MYC3-like isoform X3
Swissprot	Q9FIP9	1e-135	MYC3_ARATH; Transcription factor MYC3
TrEMBL	A0A078J098	0.0	A0A078J098_BRANA; BnaA06g40330D protein
STRING	Bra024998.1-P	1e-159	(Brassica rapa)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM1265	28	96

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G46760.1	1e-124	bHLH family protein

Publications ? help Back to Top
Chico JM, et al. Repression of Jasmonate-Dependent Defenses by Shade Involves Differential Regulation of Protein Stability of MYC Transcription Factors and Their JAZ Repressors in Arabidopsis. Plant Cell, 2014. 26(5): p. 1967-1980 [PMID:24824488] Chalhoub B, et al. Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome. Science, 2014. 345(6199): p. 950-3 [PMID:25146293] Gasperini D, et al. Multilayered Organization of Jasmonate Signalling in the Regulation of Root Growth. PLoS Genet., 2015. 11(6): p. e1005300 [PMID:26070206] Qi T, et al. Regulation of Jasmonate-Induced Leaf Senescence by Antagonism between bHLH Subgroup IIIe and IIId Factors in Arabidopsis. Plant Cell, 2015. 27(6): p. 1634-49 [PMID:26071420] de Torres Zabala M, et al. Novel JAZ co-operativity and unexpected JA dynamics underpin Arabidopsis defence responses to Pseudomonas syringae infection. New Phytol., 2016. 209(3): p. 1120-34 [PMID:26428397] Yu J, et al. JAZ7 negatively regulates dark-induced leaf senescence in Arabidopsis. J. Exp. Bot., 2016. 67(3): p. 751-62 [PMID:26547795] Chen X,Huang H,Qi T,Liu B,Song S New perspective of the bHLH-MYB complex in jasmonate-regulated plant fertility in arabidopsis. Plant Signal Behav, 2016. 11(2): p. e1135280 [PMID:26829586] Schmiesing A,Emonet A,Gouhier-Darimont C,Reymond P Arabidopsis MYC Transcription Factors Are the Target of Hormonal Salicylic Acid/Jasmonic Acid Cross Talk in Response to Pieris brassicae Egg Extract. Plant Physiol., 2016. 170(4): p. 2432-43 [PMID:26884488] Thatcher LF, et al. Characterization of a JAZ7 activation-tagged Arabidopsis mutant with increased susceptibility to the fungal pathogen Fusarium oxysporum. J. Exp. Bot., 2016. 67(8): p. 2367-86 [PMID:26896849] Gao C, et al. MYC2, MYC3, and MYC4 function redundantly in seed storage protein accumulation in Arabidopsis. Plant Physiol. Biochem., 2016. 108: p. 63-70 [PMID:27415132] Gimenez-Ibanez S, et al. JAZ2 controls stomata dynamics during bacterial invasion. New Phytol., 2017. 213(3): p. 1378-1392 [PMID:28005270] Zhang F, et al. Structural insights into alternative splicing-mediated desensitization of jasmonate signaling. Proc. Natl. Acad. Sci. U.S.A., 2017. 114(7): p. 1720-1725 [PMID:28137867] Lian TF,Xu YP,Li LF,Su XD Crystal Structure of Tetrameric Arabidopsis MYC2 Reveals the Mechanism of Enhanced Interaction with DNA. Cell Rep, 2017. 19(7): p. 1334-1342 [PMID:28514654] Wang H, et al. The bHLH Transcription Factors MYC2, MYC3, and MYC4 Are Required for Jasmonate-Mediated Inhibition of Flowering in Arabidopsis. Mol Plant, 2017. 10(11): p. 1461-1464 [PMID:28827172] Song S, et al. MYC5 is Involved in Jasmonate-Regulated Plant Growth, Leaf Senescence and Defense Responses. Plant Cell Physiol., 2017. 58(10): p. 1752-1763 [PMID:29017003] Li B, et al. Network-Guided Discovery of Extensive Epistasis between Transcription Factors Involved in Aliphatic Glucosinolate Biosynthesis. Plant Cell, 2018. 30(1): p. 178-195 [PMID:29317470] Han X, et al. Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons. Plant Physiol., 2018. 176(4): p. 2871-2885 [PMID:29496884]