PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G67110.1

Common Name

ALC, BHLH73, EN98, K21H1.7

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bHLH

Protein Properties

Length: 210aa MW: 22963 Da PI: 9.7384

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G67110.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	50.6	3.5e-16	97	143	4	55
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 hn E++RR++iN+++ L++l+P++ K +Ka++L +A+eY+k+Lq AT5G67110.1 97 FHNLSEKKRRSKINEKMKALQKLIPNS-----NKTDKASMLDEAIEYLKQLQ 143 6***********************7.....5****************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50888	16.977	93	142	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	1.3E-13	97	143	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	2.09E-19	97	165	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	3.2E-19	98	150	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	4.40E-17	98	147	No hit	No description
SMART	SM00353	4.2E-18	99	148	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0010047	Biological Process	fruit dehiscence
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020100	anatomy	hypocotyl
PO:0025022	anatomy	collective leaf structure
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 210 aa Download sequence Send to blast
MGDSDVGDRL PPPSSSDELS SFLRQILSRT PTAQPSSPPK STNVSSAETF FPSVSGGAVS 60 SVGYGVSETG QDKYAFEHKR SGAKQRNSLK RNIDAQFHNL SEKKRRSKIN EKMKALQKLI 120 PNSNKTDKAS MLDEAIEYLK QLQLQVQTLA VMNGLGLNPM RLPQVPPPTH TRINETLEQD 180 LNLETLLAAP HSLEPAKTSQ GMCFSTATLL

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	102	107	KKRRSK

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.28825	0.0	flower\| inflorescence

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	247035_at	0.0
Expression Atlas	AT5G67110	-
AtGenExpress	AT5G67110	-
ATTED-II	AT5G67110	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems, and flowers. Confined to the valve margins of the silique. {ECO:0000269\|PubMed:11747817, ECO:0000269\|PubMed:12679534}.

Functional Description ? help Back to Top
Source	Description
TAIR	encodes a myc/bHLH transcription factor-like protein. Gene product is involved in fruit dehiscence. Mutant siliques fail to dehisce.
UniProt	Required for the dehiscence of fruit, especially for the separation of the valve cells from the replum. Promotes the differentiation of a strip of labile nonlignified cells sandwiched between layers of lignified cells. {ECO:0000269\|PubMed:11747817}.

Function -- GeneRIF ? help Back to Top
Over-expression of ALCATRAZ can partially compensate for a loss of SPATULA function, and over-expression of SPATULA can fully compensate for loss of ALCATRAZ function. [PMID: 21801252]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G67110.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT2G42830 (A), AT3G58780 (A), AT5G02030 (R), AT5G60910 (R)

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT1G14920
IntAct	Search Q9FHA2

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G67110

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	BT028946	0.0	BT028946.1 Arabidopsis thaliana At5g67110 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_201512.1	1e-153	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Swissprot	Q9FHA2	1e-154	ALC_ARATH; Transcription factor ALC
TrEMBL	Q680B1	1e-151	Q680B1_ARATH; Putative bHLH transcription factor (BHLH073/ALCATRAZ)
TrEMBL	Q8LFU9	1e-151	Q8LFU9_ARATH; Uncharacterized protein
STRING	AT5G67110.1	1e-153	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM13982	17	24
Representative plant	OGRP258	16	128

Link Out ? help Back to Top
Phytozome	AT5G67110.1
Entrez Gene	836846
iHOP	AT5G67110
wikigenes	AT5G67110

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Rajani S,Sundaresan V The Arabidopsis myc/bHLH gene ALCATRAZ enables cell separation in fruit dehiscence. Curr. Biol., 2001. 11(24): p. 1914-22 [PMID:11747817] Heim MA, et al. The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol. Biol. Evol., 2003. 20(5): p. 735-47 [PMID:12679534] Toledo-Ortiz G,Huq E,Quail PH The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell, 2003. 15(8): p. 1749-70 [PMID:12897250] Robles P,Pelaz S Flower and fruit development in Arabidopsis thaliana. Int. J. Dev. Biol., 2005. 49(5-6): p. 633-43 [PMID:16096970] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Mitsuda N,Ohme-Takagi M NAC transcription factors NST1 and NST3 regulate pod shattering in a partially redundant manner by promoting secondary wall formation after the establishment of tissue identity. Plant J., 2008. 56(5): p. 768-78 [PMID:18657234] Wang F,Shi DQ,Liu J,Yang WC Novel nuclear protein ALC-INTERACTING PROTEIN1 is expressed in vascular and mesocarp cells in Arabidopsis. J Integr Plant Biol, 2008. 50(7): p. 918-27 [PMID:18713402] Mummenhoff K,Polster A,M Lepidium as a model system for studying the evolution of fruit development in Brassicaceae. J. Exp. Bot., 2009. 60(5): p. 1503-13 [PMID:19052256] Hua S, et al. Sequence, expression divergence, and complementation of homologous ALCATRAZ loci in Brassica napus. Planta, 2009. 230(3): p. 493-503 [PMID:19504267] Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Irish VF The flowering of Arabidopsis flower development. Plant J., 2010. 61(6): p. 1014-28 [PMID:20409275] Arnaud N, et al. Gibberellins control fruit patterning in Arabidopsis thaliana. Genes Dev., 2010. 24(19): p. 2127-32 [PMID:20889713] Moran CN,Halliday KJ Fruit development: new directions for an old pathway. Curr. Biol., 2010. 20(24): p. R1081-3 [PMID:21172630] Arabidopsis Interactome Mapping Consortium Evidence for network evolution in an Arabidopsis interactome map. Science, 2011. 333(6042): p. 601-7 [PMID:21798944] Groszmann M,Paicu T,Alvarez JP,Swain SM,Smyth DR SPATULA and ALCATRAZ, are partially redundant, functionally diverging bHLH genes required for Arabidopsis gynoecium and fruit development. Plant J., 2011. 68(5): p. 816-29 [PMID:21801252] Avino M,Kramer EM,Donohue K,Hammel AJ,Hall JC Understanding the basis of a novel fruit type in Brassicaceae: conservation and deviation in expression patterns of six genes. Evodevo, 2012. 3(1): p. 20 [PMID:22943452] Lenser T,Thei Conservation of fruit dehiscence pathways between Lepidium campestre and Arabidopsis thaliana sheds light on the regulation of INDEHISCENT. Plant J., 2013. 76(4): p. 545-56 [PMID:24004048] Pabón-Mora N,Wong GK,Ambrose BA Evolution of fruit development genes in flowering plants. Front Plant Sci, 2014. 5: p. 300 [PMID:25018763] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Pfannebecker KC,Lange M,Rupp O,Becker A Seed Plant-Specific Gene Lineages Involved in Carpel Development. Mol. Biol. Evol., 2017. 34(4): p. 925-942 [PMID:28087776] Zumajo-Cardona C,Ambrose BA,Pabón-Mora N Evolution of the SPATULA/ALCATRAZ gene lineage and expression analyses in the basal eudicot, Bocconia frutescens L. (Papaveraceae). Evodevo, 2017. 8: p. 5 [PMID:28331573] He H, et al. CELLULASE6 and MANNANASE7 Affect Cell Differentiation and Silique Dehiscence. Plant Physiol., 2018. 176(3): p. 2186-2201 [PMID:29348141]