PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G66730.1
Common NameENY, IDD1, MSN2.12
Organism
Arabidopsis thaliana
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family C2H2
Protein Properties Length: 500aa    MW: 52626.4 Da    PI: 8.8201
Description C2H2-like zinc finger protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G66730.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1zf-C2H217.21.4e-056183123
                 EEETTTTEEESSHHHHHHHHHHT CS
      zf-C2H2  1 ykCpdCgksFsrksnLkrHirtH 23
                 ++C++C+k F r  nL+ H r H
  AT5G66730.1 61 FVCEICNKGFQRDQNLQLHRRGH 83
                 89*******************88 PP
2zf-C2H211.90.0007137159123
                  EEETTTTEEESSHHHHHHHHHHT CS
      zf-C2H2   1 ykCpdCgksFsrksnLkrHirtH 23 
                  +kC++C+k +  +s+ k H + +
  AT5G66730.1 137 WKCEKCSKKYAVQSDWKAHSKIC 159
                  58*****************9876 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF576671.71E-66083No hitNo description
Gene3DG3DSA:3.30.160.601.6E-56083IPR013087Zinc finger C2H2-type/integrase DNA-binding domain
SMARTSM003550.0176183IPR015880Zinc finger, C2H2-like
PROSITE profilePS5015710.9496183IPR007087Zinc finger, C2H2
PfamPF121718.0E-56183IPR022755Zinc finger, double-stranded RNA binding
PROSITE patternPS0002806383IPR007087Zinc finger, C2H2
SMARTSM0035581102132IPR015880Zinc finger, C2H2-like
Gene3DG3DSA:3.30.160.605.4E-4125158IPR013087Zinc finger C2H2-type/integrase DNA-binding domain
SuperFamilySSF576671.71E-6132157No hitNo description
SMARTSM00355140137157IPR015880Zinc finger, C2H2-like
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009937Biological Processregulation of gibberellic acid mediated signaling pathway
GO:0010029Biological Processregulation of seed germination
GO:0010431Biological Processseed maturation
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0008270Molecular Functionzinc ion binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000016anatomylateral root primordium
PO:0000025anatomyroot tip
PO:0000027anatomylateral root tip
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
PO:0007632developmental stageseed maturation stage
Sequence ? help Back to Top
Protein Sequence    Length: 500 aa     Download sequence    Send to blast
MPVDLDNSST VSGDASVSST GNQNLTPKSV GKKKRNLPGM PDPDAEVIAL SPKTLMATNR  60
FVCEICNKGF QRDQNLQLHR RGHNLPWKLR QRSTKEVRKK VYVCPVSGCV HHDPSRALGD  120
LTGIKKHFCR KHGEKKWKCE KCSKKYAVQS DWKAHSKICG TKEYKCDCGT LFSRRDSFIT  180
HRAFCDALAE ESAKNHTQSK KLYPETVTRK NPEIEQKSPA AVESSPSLPP SSPPSVAIAP  240
APAISVETES VKIISSSVLP IQNSPESQEN NNHPEVIIEE ASRTIGFNVS SSDLSNDHSN  300
NNGGYAGLFV SSTASPSLYA SSTASPSLFA PSSSMEPISL CLSTNPSLFG PTIRDPPHFL  360
TPLPPQPAMS ATALLQKAAQ MGSTGSGGSL LRGLGIVSTT SSSMELSNHD ALSLAPGLGL  420
GLPCSSGGSG SGLKELMMGN SSVFGPKQTT LDFLGLGRAV GNGGNTGGGL SALLTSIGGG  480
GGIDLFGSGE FSGKDIGRSS
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5b3h_C5e-35133194364Zinc finger protein JACKDAW
5b3h_F5e-35133194364Zinc finger protein JACKDAW
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
18799KLRQRSTKEVRKK
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.276430.0bud| flower| leaf| root| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO425688180.0
Genevisible247054_at0.0
Expression AtlasAT5G66730-
AtGenExpressAT5G66730-
ATTED-IIAT5G66730-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Strongly up-regulated during seed development. {ECO:0000269|PubMed:21571950}.
UniprotTISSUE SPECIFICITY: At 3 days post anthesis (DPA), expressed in the chalazal endosperm region. By 6 DPA, expressed in the endosperm and embryo. In fully germinated seed, strongest expression in the root tip and not detected in the cotyledons. In 4-days old seedlings, restricted to the vasculature of the cotyledons, the shoot apical meristem region, and the root tip. By 8 days, restricted to newly emerged leaves. {ECO:0000269|PubMed:21571950}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor promoting the transition to germination by regulating light and hormonal signaling during seed maturation (PubMed:21571950). Acts as a positive regulator of phytochrome and/or gibberellin action (PubMed:21571950, PubMed:25035403). {ECO:0000269|PubMed:21571950, ECO:0000269|PubMed:25035403}.
Function -- GeneRIF ? help Back to Top
  1. The physical interaction of ENY with DELLAs and an ENY-triggered accumulation of DELLA transcripts during maturation support that ENY mediates gibberellin effects to balance abscisic acid-promoted maturation during late seed development.
    [PMID: 21571950]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00592DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G66730.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Interaction ? help Back to Top
Source Intact With
BioGRIDAT1G14920, AT1G66350
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G66730
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY0998590.0AY099859.1 Arabidopsis thaliana zinc finger protein (At5g66730) mRNA, complete cds.
GenBankBT0003100.0BT000310.1 Arabidopsis thaliana zinc finger protein (At5g66730) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_201474.10.0C2H2-like zinc finger protein
SwissprotQ9LVQ70.0IDD1_ARATH; Zinc finger protein ENHYDROUS
TrEMBLA0A178UMT20.0A0A178UMT2_ARATH; IDD1
STRINGAT5G66730.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM15528306
Representative plantOGRP9516242
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  3. Englbrecht CC,Schoof H,B
    Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome.
    BMC Genomics, 2004. 5(1): p. 39
    [PMID:15236668]
  4. Colasanti J, et al.
    The maize INDETERMINATE1 flowering time regulator defines a highly conserved zinc finger protein family in higher plants.
    BMC Genomics, 2006. 7: p. 158
    [PMID:16784536]
  5. Morita MT, et al.
    A C2H2-type zinc finger protein, SGR5, is involved in early events of gravitropism in Arabidopsis inflorescence stems.
    Plant J., 2006. 47(4): p. 619-28
    [PMID:16813575]
  6. Bassel GW, et al.
    Elucidating the germination transcriptional program using small molecules.
    Plant Physiol., 2008. 147(1): p. 143-55
    [PMID:18359847]
  7. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  8. Feurtado JA, et al.
    The Arabidopsis C2H2 zinc finger INDETERMINATE DOMAIN1/ENHYDROUS promotes the transition to germination by regulating light and hormonal signaling during seed maturation.
    Plant Cell, 2011. 23(5): p. 1772-94
    [PMID:21571950]
  9. Gaudinier A, et al.
    Enhanced Y1H assays for Arabidopsis.
    Nat. Methods, 2011. 8(12): p. 1053-5
    [PMID:22037706]
  10. Fukazawa J, et al.
    DELLAs function as coactivators of GAI-ASSOCIATED FACTOR1 in regulation of gibberellin homeostasis and signaling in Arabidopsis.
    Plant Cell, 2014. 26(7): p. 2920-38
    [PMID:25035403]
  11. Moreno-Risueno MA, et al.
    Transcriptional control of tissue formation throughout root development.
    Science, 2015. 350(6259): p. 426-30
    [PMID:26494755]