PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G65210.1

Common Name

BZIP47, MQN23.15, TGA1

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bZIP

Protein Properties

Length: 368aa MW: 42063.6 Da PI: 7.7595

Description

bZIP family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G65210.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	bZIP_1	35.5	2.2e-11	82	118	3	39
XXCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS bZIP_1 3 elkrerrkqkNReAArrsRqRKkaeieeLeekvkeLe 39 ++k +rr+++NReAAr+sR+RKka++++Le+ +L AT5G65210.1 82 PDKIQRRLAQNREAARKSRLRKKAYVQQLETSRLKLI 118 6899**************************8655554 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SMART	SM00338	2.1E-6	80	143	IPR004827	Basic-leucine zipper domain
Pfam	PF00170	2.1E-8	82	113	IPR004827	Basic-leucine zipper domain
PROSITE profile	PS50217	8.772	82	124	IPR004827	Basic-leucine zipper domain
CDD	cd14708	3.12E-22	84	135	No hit	No description
SuperFamily	SSF57959	2.56E-7	84	119	No hit	No description
Gene3D	G3DSA:1.20.5.170	1.9E-7	86	112	No hit	No description
PROSITE pattern	PS00036	0	87	102	IPR004827	Basic-leucine zipper domain
Pfam	PF14144	5.1E-30	170	245	IPR025422	Transcription factor TGA like domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0042742	Biological Process	defense response to bacterium
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0005516	Molecular Function	calmodulin binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0020030	anatomy	cotyledon
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 368 aa Download sequence Send to blast
MNSTSTHFVP PRRVGIYEPV HQFGMWGESF KSNISNGTMN TPNHIIIPNN QKLDNNVSED 60 TSHGTAGTPH MFDQEASTSR HPDKIQRRLA QNREAARKSR LRKKAYVQQL ETSRLKLIQL 120 EQELDRARQQ GFYVGNGIDT NSLGFSETMN PGIAAFEMEY GHWVEEQNRQ ICELRTVLHG 180 HINDIELRSL VENAMKHYFE LFRMKSSAAK ADVFFVMSGM WRTSAERFFL WIGGFRPSDL 240 LKVLLPHFDV LTDQQLLDVC NLKQSCQQAE DALTQGMEKL QHTLADCVAA GQLGEGSYIP 300 QVNSAMDRLE ALVSFVNQAD HLRHETLQQM YRILTTRQAA RGLLALGEYF QRLRALSSSW 360 ATRHREPT

Sequence ? help Back to Top

Protein Sequence Length: 368 aa Download sequence Send to blast

MNSTSTHFVP PRRVGIYEPV HQFGMWGESF KSNISNGTMN TPNHIIIPNN QKLDNNVSED  60
TSHGTAGTPH MFDQEASTSR HPDKIQRRLA QNREAARKSR LRKKAYVQQL ETSRLKLIQL  120
EQELDRARQQ GFYVGNGIDT NSLGFSETMN PGIAAFEMEY GHWVEEQNRQ ICELRTVLHG  180
HINDIELRSL VENAMKHYFE LFRMKSSAAK ADVFFVMSGM WRTSAERFFL WIGGFRPSDL  240
LKVLLPHFDV LTDQQLLDVC NLKQSCQQAE DALTQGMEKL QHTLADCVAA GQLGEGSYIP  300
QVNSAMDRLE ALVSFVNQAD HLRHETLQQM YRILTTRQAA RGLLALGEYF QRLRALSSSW  360
ATRHREPT

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.20818	0.0	leaf\| root

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	247199_at	0.0
Expression Atlas	AT5G65210	-
AtGenExpress	AT5G65210	-
ATTED-II	AT5G65210	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Predominantly expressed in roots. {ECO:0000269\|PubMed:8628224}.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes TGA1, a redox-controlled regulator of systemic acquired resistance. TGA1 targets the activation sequence-1 (as-1) element of the promoter region of defense proteins. TGA1 are S-nitrosylated.
UniProt	Transcriptional activator that binds specifically to the DNA sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis elements mediate auxin- and salicylic acid-inducible transcription. May be involved in the induction of the systemic acquired resistance (SAR) via its interaction with NPR1. Could also bind to the Hex-motif (5'-TGACGTGG-3') another cis-acting element found in plant histone promoters.

Function -- GeneRIF ? help Back to Top
S-nitrosoglutathione enhances the DNA binding activity of TGA1 in presence of NPR1 and that both proteins, NPR1 and TGA1, are S-nitrosylated after treatment with the NO donor. [PMID: 20716698] clade I TGA factors as positive regulators of one or more ER-related secretion pathways [PMID: 24086773] TGA1 and TGA4 as important regulatory factors of the nitrate response in Arabidopsis roots. [PMID: 25039575] Our study showed that TGA1 and TGA4 regulate Pip and SA biosynthesis by modulating the expression of SARD1 and CBP60g. [PMID: 28898429]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00586	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G65210.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- Hormone ? help Back to Top
Source	Hormone
AHD	salicylic acid

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT5G65210
IntAct	Search Q39237

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G65210

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK317488	0.0	AK317488.1 Arabidopsis thaliana AT5G65210 mRNA, complete cds, clone: RAFL23-11-L19.
GenBank	AY087049	0.0	AY087049.1 Arabidopsis thaliana clone 31032 mRNA, complete sequence.
GenBank	AY099593	0.0	AY099593.1 Arabidopsis thaliana DNA binding protein TGA1a-like protein (At5g65210) mRNA, complete cds.
GenBank	AY128838	0.0	AY128838.1 Arabidopsis thaliana DNA binding protein TGA1a-like protein (At5g65210) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001032147.1	0.0	bZIP transcription factor family protein
Refseq	NP_001032148.1	0.0	bZIP transcription factor family protein
Refseq	NP_001032149.1	0.0	bZIP transcription factor family protein
Refseq	NP_001318881.1	0.0	bZIP transcription factor family protein
Refseq	NP_201324.1	0.0	bZIP transcription factor family protein
Refseq	NP_851273.1	0.0	bZIP transcription factor family protein
Swissprot	Q39237	0.0	TGA1_ARATH; Transcription factor TGA1
TrEMBL	A0A384L8H7	0.0	A0A384L8H7_ARATH; TGA1
TrEMBL	B9DGG1	0.0	B9DGG1_ARATH; AT5G65210 protein
STRING	AT5G65210.2	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2976	27	66
Representative plant	OGRP2679	11	32

Link Out ? help Back to Top
Phytozome	AT5G65210.1
Entrez Gene	836646
iHOP	AT5G65210
wikigenes	AT5G65210

Publications ? help Back to Top
Zhou JM, et al. NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid. Mol. Plant Microbe Interact., 2000. 13(2): p. 191-202 [PMID:10659709] Després C,DeLong C,Glaze S,Liu E,Fobert PR The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors. Plant Cell, 2000. 12(2): p. 279-90 [PMID:10662863] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Weigel RR,Bäuscher C,Pfitzner AJ,Pfitzner UM NIMIN-1, NIMIN-2 and NIMIN-3, members of a novel family of proteins from Arabidopsis that interact with NPR1/NIM1, a key regulator of systemic acquired resistance in plants. Plant Mol. Biol., 2001. 46(2): p. 143-60 [PMID:11442055] Reddy VS,Ali GS,Reddy AS Genes encoding calmodulin-binding proteins in the Arabidopsis genome. J. Biol. Chem., 2002. 277(12): p. 9840-52 [PMID:11782485] Jakoby M, et al. bZIP transcription factors in Arabidopsis. Trends Plant Sci., 2002. 7(3): p. 106-11 [PMID:11906833] Després C, et al. The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1. Plant Cell, 2003. 15(9): p. 2181-91 [PMID:12953119] Schindler U,Beckmann H,Cashmore AR TGA1 and G-box binding factors: two distinct classes of Arabidopsis leucine zipper proteins compete for the G-box-like element TGACGTGG. Plant Cell, 1992. 4(10): p. 1309-19 [PMID:1446171] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Zhang Y, et al. Negative regulation of defense responses in Arabidopsis by two NPR1 paralogs. Plant J., 2006. 48(5): p. 647-56 [PMID:17076807] Kesarwani M,Yoo J,Dong X Genetic interactions of TGA transcription factors in the regulation of pathogenesis-related genes and disease resistance in Arabidopsis. Plant Physiol., 2007. 144(1): p. 336-46 [PMID:17369431] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Li S, et al. Nuclear activity of ROXY1, a glutaredoxin interacting with TGA factors, is required for petal development in Arabidopsis thaliana. Plant Cell, 2009. 21(2): p. 429-41 [PMID:19218396] Lindermayr C,Sell S,M Redox regulation of the NPR1-TGA1 system of Arabidopsis thaliana by nitric oxide. Plant Cell, 2010. 22(8): p. 2894-907 [PMID:20716698] Hanada K, et al. Functional compensation of primary and secondary metabolites by duplicate genes in Arabidopsis thaliana. Mol. Biol. Evol., 2011. 28(1): p. 377-82 [PMID:20736450] Arabidopsis Interactome Mapping Consortium Evidence for network evolution in an Arabidopsis interactome map. Science, 2011. 333(6042): p. 601-7 [PMID:21798944] Klopffleisch K, et al. Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis. Mol. Syst. Biol., 2011. 7: p. 532 [PMID:21952135] Gaudinier A, et al. Enhanced Y1H assays for Arabidopsis. Nat. Methods, 2011. 8(12): p. 1053-5 [PMID:22037706] Shearer HL, et al. Arabidopsis clade I TGA transcription factors regulate plant defenses in an NPR1-independent fashion. Mol. Plant Microbe Interact., 2012. 25(11): p. 1459-68 [PMID:22876961] Wang L,Fobert PR Arabidopsis clade I TGA factors regulate apoplastic defences against the bacterial pathogen Pseudomonas syringae through endoplasmic reticulum-based processes. PLoS ONE, 2013. 8(9): p. e77378 [PMID:24086773] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Alvarez JM, et al. Systems approach identifies TGA1 and TGA4 transcription factors as important regulatory components of the nitrate response of Arabidopsis thaliana roots. Plant J., 2014. 80(1): p. 1-13 [PMID:25039575] Herrera-Vásquez A, et al. Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis. Plant Mol. Biol. Rep., 2018. [PMID:26696694] Gutsche N,Zachgo S The N-Terminus of the Floral Arabidopsis TGA Transcription Factor PERIANTHIA Mediates Redox-Sensitive DNA-Binding. PLoS ONE, 2016. 11(4): p. e0153810 [PMID:27128442] Sun T, et al. TGACG-BINDING FACTOR 1 (TGA1) and TGA4 regulate salicylic acid and pipecolic acid biosynthesis by modulating the expression of SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1) and CALMODULIN-BINDING PROTEIN 60g (CBP60g). New Phytol., 2018. 217(1): p. 344-354 [PMID:28898429] Lam E,Lam YK Binding site requirements and differential representation of TGF factors in nuclear ASF-1 activity. Nucleic Acids Res., 1995. 23(18): p. 3778-85 [PMID:7479010] de Pater S,Pham K,Memelink J,Kijne J Binding specificity and tissue-specific expression pattern of the Arabidopsis bZIP transcription factor TGA2. Mol. Gen. Genet., 1996. 250(2): p. 237-9 [PMID:8628224]