PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G65050.2
Common NameAGL31, MAF2
Organism
Arabidopsis thaliana
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family MIKC_MADS
Protein Properties Length: 178aa    MW: 19980.7 Da    PI: 6.5584
Description AGAMOUS-like 31
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G65050.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1SRF-TF65.17.5e-21956148
                 S---SHHHHHHHHHHHHHHHHHHHHHHHHHHT-EEEEEEE-TTSEEEE CS
       SRF-TF  1 krienksnrqvtfskRrngilKKAeELSvLCdaevaviifsstgklye 48
                 krienks rqvtfskRrng++ KA  LS+LC+  +av+++s +gkly 
  AT5G65050.2  9 KRIENKSSRQVTFSKRRNGLIEKARQLSILCESSIAVLVVSGSGKLYK 56
                 79********************************************96 PP
2K-box39.22.9e-14921642398
        K-box  23 kLkkeienLqreqRhllGedLesLslkeLqqLeqqLekslkkiRskKnellleqieelqkkekelqeenkaLrkkl 98 
                   Lk+ +e  q+   +l   ++++ s+  L +Le+qLe++l+  R++K+el++ +++ lqk e+ l+een++L +++
  AT5G65050.2  92 PLKELLEIVQS---KLEESNVDNASVDTLISLEEQLETALSVTRARKTELMMGEVKSLQKTENLLREENQTLASQV 164
                  55555555554...47778899999***********************************************9987 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF554551.23E-25174IPR002100Transcription factor, MADS-box
PROSITE profilePS5006627.74161IPR002100Transcription factor, MADS-box
SMARTSM004327.7E-31160IPR002100Transcription factor, MADS-box
CDDcd002651.34E-31278No hitNo description
PRINTSPR004041.7E-23323IPR002100Transcription factor, MADS-box
PROSITE patternPS003500357IPR002100Transcription factor, MADS-box
PfamPF003197.3E-211055IPR002100Transcription factor, MADS-box
PRINTSPR004041.7E-232338IPR002100Transcription factor, MADS-box
PRINTSPR004041.7E-233859IPR002100Transcription factor, MADS-box
PROSITE profilePS5129711.00380170IPR002487Transcription factor, K-box
PfamPF014861.3E-1097164IPR002487Transcription factor, K-box
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009910Biological Processnegative regulation of flower development
GO:0010221Biological Processnegative regulation of vernalization response
GO:0030154Biological Processcell differentiation
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000293anatomyguard cell
PO:0006339anatomyjuvenile vascular leaf
PO:0006340anatomyadult vascular leaf
PO:0009001anatomyfruit
PO:0009005anatomyroot
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009049anatomyinflorescence
Sequence ? help Back to Top
Protein Sequence    Length: 178 aa     Download sequence    Send to blast
MGRKKVEIKR IENKSSRQVT FSKRRNGLIE KARQLSILCE SSIAVLVVSG SGKLYKSASG  60
DNMSKIIDRY EIHHADELEA LDLAEKTRNY LPLKELLEIV QSKLEESNVD NASVDTLISL  120
EEQLETALSV TRARKTELMM GEVKSLQKTE NLLREENQTL ASQVTKTSLE ANSSVDTQ
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5f28_A2e-17185192MEF2C
5f28_B2e-17185192MEF2C
5f28_C2e-17185192MEF2C
5f28_D2e-17185192MEF2C
6byy_A3e-17170169MEF2 CHIMERA
6byy_B3e-17170169MEF2 CHIMERA
6byy_C3e-17170169MEF2 CHIMERA
6byy_D3e-17170169MEF2 CHIMERA
6bz1_A3e-17170169MEF2 CHIMERA
6bz1_B3e-17170169MEF2 CHIMERA
6bz1_C3e-17170169MEF2 CHIMERA
6bz1_D3e-17170169MEF2 CHIMERA
Search in ModeBase
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.492250.0bud| flower| inflorescence| leaf| root
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible247217_s_at1e-159
Expression AtlasAT5G65050-
AtGenExpressAT5G65050-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in most plant tissues, roots, seedlings, leaves, stems, inflorescences, pollen, siliques and flowers. {ECO:0000269|PubMed:11115127, ECO:0000269|PubMed:12837945, ECO:0000269|PubMed:12949148}.
Functional Description ? help Back to Top
Source Description
TAIROriginally published as Agamous like MADS-box protein AGL31. One of a group of MADS box genes involved in control of flowering time. Four variant sequences have been identified for this locus but have not been characterized for differences in expression pattern and/or function.
UniProtProbable transcription factor that prevents vernalization by short periods of cold. Acts as a floral repressor. {ECO:0000269|PubMed:12724541, ECO:0000269|PubMed:19139056, ECO:0000269|PubMed:20551443}.
Function -- GeneRIF ? help Back to Top
  1. Association studies support a correlation between the described MAF2-MAF3 gene rearrangements and flowering time variation in the species
    [PMID: 19139056]
  2. molecular basis of MAF2 gene function and role of alternative MAF2 transcripts in flowering time modulation
    [PMID: 23111501]
  3. FLM and MAF2 repress flowering in parallel at low temperature. [MAF2]
    [PMID: 25955034]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G65050.2
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Requires EARLY FLOWERING 7 (ELF7) and ELF8 to be expressed. Up-regulated by HUA2. {ECO:0000269|PubMed:15520273, ECO:0000269|PubMed:15659097}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT2G45660(R)
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G65050
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF3126670.0AF312667.1 Arabidopsis thaliana MADS-box protein AGL31 (AGL31) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001078798.11e-124AGAMOUS-like 31
SwissprotQ9FPN71e-115AGL31_ARATH; Agamous-like MADS-box protein AGL31
TrEMBLA0A178UKR61e-112A0A178UKR6_ARATH; MAF2
STRINGAT5G65050.31e-113(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Alvarez-Buylla ER, et al.
    MADS-box gene evolution beyond flowers: expression in pollen, endosperm, guard cells, roots and trichomes.
    Plant J., 2000. 24(4): p. 457-66
    [PMID:11115127]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Ratcliffe OJ,Kumimoto RW,Wong BJ,Riechmann JL
    Analysis of the Arabidopsis MADS AFFECTING FLOWERING gene family: MAF2 prevents vernalization by short periods of cold.
    Plant Cell, 2003. 15(5): p. 1159-69
    [PMID:12724541]
  4. Parenicová L, et al.
    Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: new openings to the MADS world.
    Plant Cell, 2003. 15(7): p. 1538-51
    [PMID:12837945]
  5. Bae MS,Cho EJ,Choi EY,Park OK
    Analysis of the Arabidopsis nuclear proteome and its response to cold stress.
    Plant J., 2003. 36(5): p. 652-63
    [PMID:14617066]
  6. Kim SY, et al.
    Establishment of the vernalization-responsive, winter-annual habit in Arabidopsis requires a putative histone H3 methyl transferase.
    Plant Cell, 2005. 17(12): p. 3301-10
    [PMID:16258034]
  7. Andersson CR, et al.
    The FLX gene of Arabidopsis is required for FRI-dependent activation of FLC expression.
    Plant Cell Physiol., 2008. 49(2): p. 191-200
    [PMID:18156133]
  8. Alexandre CM,Hennig L
    FLC or not FLC: the other side of vernalization.
    J. Exp. Bot., 2008. 59(6): p. 1127-35
    [PMID:18390846]
  9. Caicedo AL,Richards C,Ehrenreich IM,Purugganan MD
    Complex rearrangements lead to novel chimeric gene fusion polymorphisms at the Arabidopsis thaliana MAF2-5 flowering time gene cluster.
    Mol. Biol. Evol., 2009. 26(3): p. 699-711
    [PMID:19139056]
  10. Rosloski SM,Jali SS,Balasubramanian S,Weigel D,Grbic V
    Natural diversity in flowering responses of Arabidopsis thaliana caused by variation in a tandem gene array.
    Genetics, 2010. 186(1): p. 263-76
    [PMID:20551443]
  11. Rosloski SM, et al.
    Functional analysis of splice variant expression of MADS AFFECTING FLOWERING 2 of Arabidopsis thaliana.
    Plant Mol. Biol., 2013. 81(1-2): p. 57-69
    [PMID:23111501]
  12. Suter L,Rüegg M,Zemp N,Hennig L,Widmer A
    Gene regulatory variation mediates flowering responses to vernalization along an altitudinal gradient in Arabidopsis.
    Plant Physiol., 2014. 166(4): p. 1928-42
    [PMID:25339407]
  13. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  14. Airoldi CA,McKay M,Davies B
    MAF2 Is Regulated by Temperature-Dependent Splicing and Represses Flowering at Low Temperatures in Parallel with FLM.
    PLoS ONE, 2015. 10(5): p. e0126516
    [PMID:25955034]