PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G62020.1

Common Name

AT-HSFB2A, HSF22, HSF6, HSFB2A, MTG10.4

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

HSF

Protein Properties

Length: 299aa MW: 34050.8 Da PI: 6.0656

Description

heat shock transcription factor B2A

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G62020.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HSF_DNA-bind	115.8	2.6e-36	24	115	2	102
HHHHHHHHHCTGGGTTTSEESSSSSEEEES-HHHHHHHTHHHHSTT--HHHHHHHHHHTTEEE---SSBTTTTXTTSEEEEESXXXXXXXXXXXXXX CS HSF_DNA-bind 2 FlkklyeiledeelkeliswsengnsfvvldeeefakkvLpkyFkhsnfaSFvRQLnmYgFkkvkdeekkskskekiweFkhksFkkgkkellekik 98 Fl+k+++++ed++++++isw+e+g+sf+v+++++fak++Lpk+Fkh+nf+SFvRQLn+YgFkkv ++ weF+++ Fk+g+k+ll++i+ AT5G62020.1 24 FLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSFVRQLNTYGFKKVVPDR---------WEFSNDFFKRGEKRLLREIQ 111 9***************************************************************99.........****************** PP XXXX CS HSF_DNA-bind 99 rkks 102 r+k AT5G62020.1 112 RRKI 115 *985 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SMART	SM00415	1.1E-54	20	113	IPR000232	Heat shock factor (HSF)-type, DNA-binding
Gene3D	G3DSA:1.10.10.10	2.2E-37	21	113	IPR011991	Winged helix-turn-helix DNA-binding domain
SuperFamily	SSF46785	3.94E-34	21	113	IPR011991	Winged helix-turn-helix DNA-binding domain
Pfam	PF00447	1.1E-31	24	113	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	5.4E-19	24	47	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	5.4E-19	62	74	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PROSITE pattern	PS00434	0	63	87	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	5.4E-19	75	87	IPR000232	Heat shock factor (HSF)-type, DNA-binding

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0010200	Biological Process	response to chitin
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000037	anatomy	shoot apex
PO:0000293	anatomy	guard cell
PO:0009005	anatomy	root
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020100	anatomy	hypocotyl
PO:0025022	anatomy	collective leaf structure
PO:0025195	anatomy	pollen tube cell
PO:0025281	anatomy	pollen
PO:0001017	developmental stage	M germinated pollen stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 299 aa Download sequence Send to blast
MNSPPVDAMI TGESSSQRSI PTPFLTKTFN LVEDSSIDDV ISWNEDGSSF IVWNPTDFAK 60 DLLPKHFKHN NFSSFVRQLN TYGFKKVVPD RWEFSNDFFK RGEKRLLREI QRRKITTTHQ 120 TVVAPSSEQR NQTMVVSPSN SGEDNNNNQV MSSSPSSWYC HQTKTTGNGG LSVELLEENE 180 KLRSQNIQLN RELTQMKSIC DNIYSLMSNY VGSQPTDRSY SPGGSSSQPM EFLPAKRFSE 240 MEIEEEEEAS PRLFGVPIGL KRTRSEGVQV KTTAVVGENS DEETPWLRHY NRTNQRVCN

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5d5u_B	6e-23	6	113	12	129	Heat shock factor protein 1
5d5v_B	6e-23	6	113	12	129	Heat shock factor protein 1
5d5v_D	6e-23	6	113	12	129	Heat shock factor protein 1
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Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.5973	0.0	leaf

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	42568701	0.0
Genevisible	247509_at	0.0
Expression Atlas	AT5G62020	-
AtGenExpress	AT5G62020	-
ATTED-II	AT5G62020	-

Functional Description ? help Back to Top
Source	Description
TAIR	member of Heat Stress Transcription Factor (Hsf) family
UniProt	Transcriptional regulator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).

Function -- GeneRIF ? help Back to Top
Regulation of gene expression at the HSFB2a locus influences vegetative and gametophytic development in Arabidopsis. [PMID: 24874772]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00575	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G62020.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT2G30250 (A)

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT5G44420(A)

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G62020

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AJ251867	0.0	AJ251867.1 Arabidopsis thaliana mRNA for heat shock factor 6 (hsf6 gene).
GenBank	AK229690	0.0	AK229690.1 Arabidopsis thaliana mRNA for heat shock factor 6, complete cds, clone: RAFL21-11-D17.
GenBank	BT008542	0.0	BT008542.1 Arabidopsis thaliana clone U51273 putative heat shock factor 6 (At5g62020) mRNA, complete cds.
GenBank	BT008658	0.0	BT008658.1 Arabidopsis thaliana clone RAFL21-11-D17 (R51273) putative heat shock factor 6 (At5g62020) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_201008.2	0.0	heat shock transcription factor B2A
Swissprot	Q9SCW4	0.0	HFB2A_ARATH; Heat stress transcription factor B-2a
TrEMBL	A0A384KDZ5	0.0	A0A384KDZ5_ARATH; HSFB2A
TrEMBL	C0SVV5	0.0	C0SVV5_ARATH; Uncharacterized protein At5g62020 (Fragment)
STRING	AT5G62020.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM965	28	111
Representative plant	OGRP96	17	233

Link Out ? help Back to Top
Phytozome	AT5G62020.1
Entrez Gene	836322
iHOP	AT5G62020
wikigenes	AT5G62020

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Nover L, et al. Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need? Cell Stress Chaperones, 2001. 6(3): p. 177-89 [PMID:11599559] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Truman W,de Zabala MT,Grant M Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance. Plant J., 2006. 46(1): p. 14-33 [PMID:16553893] Lee J, et al. Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development. Plant Cell, 2007. 19(3): p. 731-49 [PMID:17337630] Ma S,Bohnert HJ Integration of Arabidopsis thaliana stress-related transcript profiles, promoter structures, and cell-specific expression. Genome Biol., 2007. 8(4): p. R49 [PMID:17408486] Libault M,Wan J,Czechowski T,Udvardi M,Stacey G Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor. Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11 [PMID:17722694] Guo J, et al. Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis. J Genet Genomics, 2008. 35(2): p. 105-18 [PMID:18407058] Wang Y, et al. Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol., 2008. 148(3): p. 1201-11 [PMID:18775970] Li S,Fu Q,Huang W,Yu D Functional analysis of an Arabidopsis transcription factor WRKY25 in heat stress. Plant Cell Rep., 2009. 28(4): p. 683-93 [PMID:19125253] Ikeda M,Ohme-Takagi M A novel group of transcriptional repressors in Arabidopsis. Plant Cell Physiol., 2009. 50(5): p. 970-5 [PMID:19324928] Kanchiswamy CN, et al. Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling. BMC Plant Biol., 2010. 10: p. 97 [PMID:20504319] Nishizawa-Yokoi A, et al. HsfA1d and HsfA1e involved in the transcriptional regulation of HsfA2 function as key regulators for the Hsf signaling network in response to environmental stress. Plant Cell Physiol., 2011. 52(5): p. 933-45 [PMID:21471117] Zhu X,Thalor SK,Takahashi Y,Berberich T,Kusano T An inhibitory effect of the sequence-conserved upstream open-reading frame on the translation of the main open-reading frame of HsfB1 transcripts in Arabidopsis. Plant Cell Environ., 2012. 35(11): p. 2014-30 [PMID:22571635] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Wunderlich M,Gross-Hardt R,Sch Heat shock factor HSFB2a involved in gametophyte development of Arabidopsis thaliana and its expression is controlled by a heat-inducible long non-coding antisense RNA. Plant Mol. Biol., 2014. 85(6): p. 541-50 [PMID:24874772] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178]