PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G59820.1

Common Name

AtZAT12, MMN10.11, RHL41, ZAT12

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

C2H2

Protein Properties

Length: 162aa MW: 17332.1 Da PI: 10.1879

Description

C2H2 family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G59820.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	zf-C2H2	13.7	0.00019	82	104	1	23
EEETTTTEEESSHHHHHHHHHHT CS zf-C2H2 1 ykCpdCgksFsrksnLkrHirtH 23 ++Cp+Cg F+ L H+r+H AT5G59820.1 82 HPCPICGVEFPMGQALGGHMRRH 104 78********************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF57667	1.21E-6	37	104	No hit	No description
Pfam	PF13912	2.5E-11	38	63	IPR007087	Zinc finger, C2H2
PROSITE profile	PS50157	8.891	39	66	IPR007087	Zinc finger, C2H2
SMART	SM00355	2.5	39	61	IPR015880	Zinc finger, C2H2-like
PROSITE pattern	PS00028	0	41	61	IPR007087	Zinc finger, C2H2
Pfam	PF13912	2.4E-11	82	105	IPR007087	Zinc finger, C2H2
PROSITE profile	PS50157	9.681	82	109	IPR007087	Zinc finger, C2H2
SMART	SM00355	0.079	82	104	IPR015880	Zinc finger, C2H2-like
PROSITE pattern	PS00028	0	84	104	IPR007087	Zinc finger, C2H2

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0006979	Biological Process	response to oxidative stress
GO:0009408	Biological Process	response to heat
GO:0009611	Biological Process	response to wounding
GO:0009631	Biological Process	cold acclimation
GO:0009643	Biological Process	photosynthetic acclimation
GO:0010200	Biological Process	response to chitin
GO:0010224	Biological Process	response to UV-B
GO:0042538	Biological Process	hyperosmotic salinity response
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0008270	Molecular Function	zinc ion binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000084	anatomy	plant sperm cell
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025195	anatomy	pollen tube cell
PO:0025281	anatomy	pollen
PO:0001016	developmental stage	L mature pollen stage
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 162 aa Download sequence Send to blast
MVAISEIKST VDVTAANCLM LLSRVGQENV DGGDQKRVFT CKTCLKQFHS FQALGGHRAS 60 HKKPNNDALS SGLMKKVKTS SHPCPICGVE FPMGQALGGH MRRHRNESGA AGGALVTRAL 120 LPEPTVTTLK KSSSGKRVAC LDLSLGMVDN LNLKLELGRT VY

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.24260	0.0	flower\| inflorescence\| leaf\| seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	247655_at	0.0
Expression Atlas	AT5G59820	-
AtGenExpress	AT5G59820	-
ATTED-II	AT5G59820	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in roots, stems and flowers. {ECO:0000269\|PubMed:9132053}.

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a zinc finger protein involved in high light and cold acclimation. Overexpression of this putative transcription factor increases the expression level of 9 cold-responsive genes and represses the expression level of 15 cold-responsive genes, including CBF genes. Also, lines overexpressing this gene exhibits a small but reproducible increase in freeze tolerance. Because of the repression of the CBF genes by the overexpression of this gene, the authors speculate that this gene may be involved in negative regulatory circuit of the CBF pathway.
UniProt	Transcriptional repressor involved in light acclimation, cold and oxidative stress responses. May regulate a collection of transcripts involved in response to high-light, cold and oxidative stress. {ECO:0000269\|PubMed:11069694, ECO:0000269\|PubMed:14722088, ECO:0000269\|PubMed:15634197, ECO:0000269\|PubMed:16183833}.

Function -- GeneRIF ? help Back to Top
Zat12 expression is activated at the transcriptional level during different abiotic stresses and in response to a wound-induced systemic signal [PMID: 16183833] Overall, our findings indicate that H(2) acts as a novel and cytoprotective regulator in coupling ZAT10/12-mediated antioxidant defence and maintenance of ion homeostasis in the improvement of Arabidopsis salt tolerance. [PMID: 23185443] ZAT12 interacts with FIT linking iron deficiency and oxidative stress responses. [PMID: 26556796]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G59820.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By H(2)O(2), cold, drought, cold or heat stresses, wounding, cucumber mosaic virus (CMV), exposure to high-intensity light and low-oxygen conditions in roots. {ECO:0000269\|PubMed:12368499, ECO:0000269\|PubMed:14722088, ECO:0000269\|PubMed:15634197, ECO:0000269\|PubMed:16183833, ECO:0000269\|PubMed:18201973, ECO:0000269\|PubMed:18922600}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G07890(A), AT2G30250(A), AT3G46090(A), AT4G25470(R), AT4G25480(R), AT4G25490(R)

Phenotype -- Disruption Phenotype ? help Back to Top
Source	Description
UniProt	DISRUPTION PHENOTYPE: No visible phenotype under normal growth condition, but enhanced tolerance to heat stress and increased sensitivity to salt stress. {ECO:0000269\|PubMed:14722088, ECO:0000269\|PubMed:16183833}.

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G59820

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AB015475	0.0	AB015475.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MMN10.
GenBank	AY050915	0.0	AY050915.1 Arabidopsis thaliana putative zinc finger protein Zat12 (At5g59820) mRNA, complete cds.
GenBank	AY133818	0.0	AY133818.1 Arabidopsis thaliana clone U11346 putative zinc finger protein Zat12 (At5g59820) mRNA, complete cds.
GenBank	CP002688	0.0	CP002688.1 Arabidopsis thaliana chromosome 5 sequence.
GenBank	X98673	0.0	X98673.1 A.thaliana mRNA for ZAT12 protein.
GenBank	X98674	0.0	X98674.1 A.thaliana zat12 gene.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_200790.1	1e-117	C2H2-type zinc finger family protein
Swissprot	Q42410	1e-118	ZAT12_ARATH; Zinc finger protein ZAT12
TrEMBL	A0A178UHQ4	1e-115	A0A178UHQ4_ARATH; ZAT12
STRING	AT5G59820.1	1e-116	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2028	18	76
Representative plant	OGRP131	15	149

Link Out ? help Back to Top
Phytozome	AT5G59820.1
Entrez Gene	836103
iHOP	AT5G59820
wikigenes	AT5G59820

Publications ? help Back to Top
Iida A,Kazuoka T,Torikai S,Kikuchi H,Oeda K A zinc finger protein RHL41 mediates the light acclimatization response in Arabidopsis. Plant J., 2000. 24(2): p. 191-203 [PMID:11069694] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Klok EJ, et al. Expression profile analysis of the low-oxygen response in Arabidopsis root cultures. Plant Cell, 2002. 14(10): p. 2481-94 [PMID:12368499] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Rizhsky L,Davletova S,Liang H,Mittler R The zinc finger protein Zat12 is required for cytosolic ascorbate peroxidase 1 expression during oxidative stress in Arabidopsis. J. Biol. Chem., 2004. 279(12): p. 11736-43 [PMID:14722088] Zhang JZ,Creelman RA,Zhu JK From laboratory to field. Using information from Arabidopsis to engineer salt, cold, and drought tolerance in crops. Plant Physiol., 2004. 135(2): p. 615-21 [PMID:15173567] Vogel JT,Zarka DG,Van Buskirk HA,Fowler SG,Thomashow MF Roles of the CBF2 and ZAT12 transcription factors in configuring the low temperature transcriptome of Arabidopsis. Plant J., 2005. 41(2): p. 195-211 [PMID:15634197] Fowler SG,Cook D,Thomashow MF Low temperature induction of Arabidopsis CBF1, 2, and 3 is gated by the circadian clock. Plant Physiol., 2005. 137(3): p. 961-8 [PMID:15728337] Ramonell K, et al. Loss-of-function mutations in chitin responsive genes show increased susceptibility to the powdery mildew pathogen Erysiphe cichoracearum. Plant Physiol., 2005. 138(2): p. 1027-36 [PMID:15923325] Davletova S,Schlauch K,Coutu J,Mittler R The zinc-finger protein Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis. Plant Physiol., 2005. 139(2): p. 847-56 [PMID:16183833] Skinner JS, et al. Mapping of barley homologs to genes that regulate low temperature tolerance in Arabidopsis. Theor. Appl. Genet., 2006. 112(5): p. 832-42 [PMID:16365758] Miller G,Mittler R Could heat shock transcription factors function as hydrogen peroxide sensors in plants? Ann. Bot., 2006. 98(2): p. 279-88 [PMID:16740587] Libault M,Wan J,Czechowski T,Udvardi M,Stacey G Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor. Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11 [PMID:17722694] Suzuki N,Bajad S,Shuman J,Shulaev V,Mittler R The transcriptional co-activator MBF1c is a key regulator of thermotolerance in Arabidopsis thaliana. J. Biol. Chem., 2008. 283(14): p. 9269-75 [PMID:18201973] Safrany J, et al. Identification of a novel cis-regulatory element for UV-B-induced transcription in Arabidopsis. Plant J., 2008. 54(3): p. 402-14 [PMID:18266923] Ascencio-Ib Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection. Plant Physiol., 2008. 148(1): p. 436-54 [PMID:18650403] Wang Y, et al. Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol., 2008. 148(3): p. 1201-11 [PMID:18775970] Mitsuya Y, et al. Spermine signaling plays a significant role in the defense response of Arabidopsis thaliana to cucumber mosaic virus. J. Plant Physiol., 2009. 166(6): p. 626-43 [PMID:18922600] Doherty CJ,Van Buskirk HA,Myers SJ,Thomashow MF Roles for Arabidopsis CAMTA transcription factors in cold-regulated gene expression and freezing tolerance. Plant Cell, 2009. 21(3): p. 972-84 [PMID:19270186] Dong CJ,Liu JY The Arabidopsis EAR-motif-containing protein RAP2.1 functions as an active transcriptional repressor to keep stress responses under tight control. BMC Plant Biol., 2010. 10: p. 47 [PMID:20230648] Cantrel C, et al. Nitric oxide participates in cold-responsive phosphosphingolipid formation and gene expression in Arabidopsis thaliana. New Phytol., 2011. 189(2): p. 415-27 [PMID:21039566] Takahashi F,Mizoguchi T,Yoshida R,Ichimura K,Shinozaki K Calmodulin-dependent activation of MAP kinase for ROS homeostasis in Arabidopsis. Mol. Cell, 2011. 41(6): p. 649-60 [PMID:21419340] Qin Y, et al. Over-expression of TaMYB33 encoding a novel wheat MYB transcription factor increases salt and drought tolerance in Arabidopsis. Mol. Biol. Rep., 2012. 39(6): p. 7183-92 [PMID:22350156] Mehterov N, et al. Oxidative stress provokes distinct transcriptional responses in the stress-tolerant atr7 and stress-sensitive loh2 Arabidopsis thaliana mutants as revealed by multi-parallel quantitative real-time PCR analysis of ROS marker and antioxidant genes. Plant Physiol. Biochem., 2012. 59: p. 20-9 [PMID:22710144] Xie Y,Mao Y,Lai D,Zhang W,Shen W H(2) enhances arabidopsis salt tolerance by manipulating ZAT10/12-mediated antioxidant defence and controlling sodium exclusion. PLoS ONE, 2012. 7(11): p. e49800 [PMID:23185443] Schweizer F,Bodenhausen N,Lassueur S,Masclaux FG,Reymond P Differential Contribution of Transcription Factors to Arabidopsis thaliana Defense Against Spodoptera littoralis. Front Plant Sci, 2013. 4: p. 13 [PMID:23382734] Hahn A, et al. Plant core environmental stress response genes are systemically coordinated during abiotic stresses. Int J Mol Sci, 2013. 14(4): p. 7617-41 [PMID:23567274] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Shi H,Chan Z The cysteine2/histidine2-type transcription factor ZINC FINGER OF ARABIDOPSIS THALIANA 6-activated C-REPEAT-BINDING FACTOR pathway is essential for melatonin-mediated freezing stress resistance in Arabidopsis. J. Pineal Res., 2014. 57(2): p. 185-91 [PMID:24962049] Munekage YN,Inoue S,Yoneda Y,Yokota A Distinct palisade tissue development processes promoted by leaf autonomous signalling and long-distance signalling in Arabidopsis thaliana. Plant Cell Environ., 2015. 38(6): p. 1116-26 [PMID:25293694] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Le CT, et al. ZINC FINGER OF ARABIDOPSIS THALIANA12 (ZAT12) Interacts with FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT) Linking Iron Deficiency and Oxidative Stress Responses. Plant Physiol., 2016. 170(1): p. 540-57 [PMID:26556796] Ben Daniel BH, et al. Identification of novel transcriptional regulators of Zat12 using comprehensive yeast one-hybrid screens. Physiol Plant, 2016. 157(4): p. 422-41 [PMID:26923089] Tang Y, et al. Oxidative stress-induced toxicity of CuO nanoparticles and related toxicogenomic responses in Arabidopsis thaliana. Environ. Pollut., 2016. 212: p. 605-614 [PMID:27016889] Corrales AR, et al. Multifaceted role of cycling DOF factor 3 (CDF3) in the regulation of flowering time and abiotic stress responses in Arabidopsis. Plant Cell Environ., 2017. 40(5): p. 748-764 [PMID:28044345] Xu J,Tran T,Padilla Marcia CS,Braun DM,Goggin FL Superoxide-responsive gene expression in Arabidopsis thaliana and Zea mays. Plant Physiol. Biochem., 2017. 117: p. 51-60 [PMID:28587993] Nguyen HM, et al. Ethanol Enhances High-Salinity Stress Tolerance by Detoxifying Reactive Oxygen Species in Arabidopsis thaliana and Rice. Front Plant Sci, 2017. 8: p. 1001 [PMID:28717360] Meissner R,Michael AJ Isolation and characterisation of a diverse family of Arabidopsis two and three-fingered C2H2 zinc finger protein genes and cDNAs. Plant Mol. Biol., 1997. 33(4): p. 615-24 [PMID:9132053]