PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G54070.1

Common Name

AT-HSFA9, HSF21, HSFA9, MJP23.4

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

HSF

Protein Properties

Length: 331aa MW: 38146.2 Da PI: 5.373

Description

heat shock transcription factor A9

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G54070.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HSF_DNA-bind	108.4	5.3e-34	72	164	2	103
HHHHHHHHHCTGGGTTTSEESSSSSEEEES-HHHHHHHTHHHHSTT--HHHHHHHHHHTTEEE---SSBTTTTXTTSEEEEESXXXXXXXXXXXXXX CS HSF_DNA-bind 2 FlkklyeiledeelkeliswsengnsfvvldeeefakkvLpkyFkhsnfaSFvRQLnmYgFkkvkdeekkskskekiweFkhksFkkgkkellekik 98 Fl+k++ei++d+ ++ ++sws + +sf+++d+ ef++++LpkyFkh+nf+SF+RQLn YgFkkv++++ weF+++ F+ gkk+ll++ik AT5G54070.1 72 FLRKTFEIVDDKVTDPVVSWSPTRKSFIIWDSYEFSENLLPKYFKHKNFSSFIRQLNSYGFKKVDSDR---------WEFANEGFQGGKKHLLKNIK 159 9******************999*************************************999.........**************** PP XXXXX CS HSF_DNA-bind 99 rkkse 103 r++++ AT5G54070.1 160 RRSKN 164 985 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SMART	SM00415	2.1E-51	68	161	IPR000232	Heat shock factor (HSF)-type, DNA-binding
SuperFamily	SSF46785	3.94E-31	69	161	IPR011991	Winged helix-turn-helix DNA-binding domain
Gene3D	G3DSA:1.10.10.10	8.9E-35	70	161	IPR011991	Winged helix-turn-helix DNA-binding domain
PRINTS	PR00056	1.4E-17	72	95	IPR000232	Heat shock factor (HSF)-type, DNA-binding
Pfam	PF00447	5.6E-30	72	161	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	1.4E-17	110	122	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PROSITE pattern	PS00434	0	111	135	IPR000232	Heat shock factor (HSF)-type, DNA-binding
PRINTS	PR00056	1.4E-17	123	135	IPR000232	Heat shock factor (HSF)-type, DNA-binding

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0007632	developmental stage	seed maturation stage

Sequence ? help Back to Top
Protein Sequence Length: 331 aa Download sequence Send to blast
MTAIPNVVDI ESSSSSLCQE TATETVTVER GSSDSSSKPD DVVLLIKEEE DDAVNLSLGF 60 WKLHEIGLIT PFLRKTFEIV DDKVTDPVVS WSPTRKSFII WDSYEFSENL LPKYFKHKNF 120 SSFIRQLNSY GFKKVDSDRW EFANEGFQGG KKHLLKNIKR RSKNTKCCNK EASTTTTETE 180 VESLKEEQSP MRLEMLKLKQ QQEESQHQMV TVQEKIHGVD TEQQHMLSFF AKLAKDQRFV 240 ERLVKKRKMK IQRELEAAEF VKKLKLLQDQ ETQKNLLDVE REFMAMAATE HNPEPDILVN 300 NQSGNTRCQL NSEDLLVDGG SMDVNGRIEI E

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ldu_A	6e-21	69	161	17	120	Heat shock factor protein 1
5d5u_B	7e-21	69	161	26	129	Heat shock factor protein 1
5d5v_B	7e-21	69	161	26	129	Heat shock factor protein 1
5d5v_D	7e-21	69	161	26	129	Heat shock factor protein 1
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	154	160	LKNIKRR

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	334188379	0.0
Genevisible	248188_at	0.0
Expression Atlas	AT5G54070	-
AtGenExpress	AT5G54070	-
ATTED-II	AT5G54070	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Starts to be expressed in seeds from 18 days after pollination (DAP) to reach the highest expression in dry seeds. Expression is strongly reduced after only 2 hours of seed imbibition, declines drastically after 6 hours, but can be detected at a very low level even after 10 hours. {ECO:0000269\|PubMed:17220197}.

Functional Description ? help Back to Top
Source	Description
TAIR	A member of Heat Stress Transcription Factor (Hsf) family. Not responding to heat stress. Is regulated by the seed-specific transcription factor ABI3. In turn, it regulates other heat stress proteins including Hsp17.4-CI, Hsp17.7-CII and Hsp101 during seed maturation.
UniProt	Seed-specific transcriptional regulator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). Seems to be specialized for the developmental expression of heat shock protein (HSP) genes during seed maturation. Activated by ABI3. {ECO:0000269\|PubMed:17220197}.

Function -- GeneRIF ? help Back to Top
Developmental expression of HsfA9 is regulated by the seed-specific transcription factor ABSCISIC ACID-INSENSITIVE3 (ABI3). [PMID: 17220197]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G54070.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT3G24650 (A)

Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source	Target Gene (A: Activate/R: Repress)
ATRM	AT1G74310(A)

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G54070

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY065226	0.0	AY065226.1 Arabidopsis thaliana unknown protein (At5g54070) mRNA, complete cds.
GenBank	AY096552	0.0	AY096552.1 Arabidopsis thaliana unknown protein (At5g54070) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_200218.1	0.0	heat shock transcription factor A9
Swissprot	Q9LVW2	0.0	HSFA9_ARATH; Heat stress transcription factor A-9
TrEMBL	A0A178UP77	0.0	A0A178UP77_ARATH; HSFA9
STRING	AT5G54070.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM7498	19	40
Representative plant	OGRP11807	5	6

Link Out ? help Back to Top
Phytozome	AT5G54070.1
Entrez Gene	835493
iHOP	AT5G54070
wikigenes	AT5G54070

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Nover L, et al. Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need? Cell Stress Chaperones, 2001. 6(3): p. 177-89 [PMID:11599559] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Kotak S,Vierling E,Bäumlein H,von Koskull-Döring P A novel transcriptional cascade regulating expression of heat stress proteins during seed development of Arabidopsis. Plant Cell, 2007. 19(1): p. 182-95 [PMID:17220197] Prieto-Dapena P,Casta The ectopic overexpression of a seed-specific transcription factor, HaHSFA9, confers tolerance to severe dehydration in vegetative organs. Plant J., 2008. 54(6): p. 1004-14 [PMID:18315542] Guo J, et al. Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis. J Genet Genomics, 2008. 35(2): p. 105-18 [PMID:18407058] Babu M,Griffiths JS,Huang TS,Wang A Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection. BMC Genomics, 2008. 9: p. 325 [PMID:18613973] Nakashima K, et al. Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy. Plant Cell Physiol., 2009. 50(7): p. 1345-63 [PMID:19541597] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178]