PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Home  TFext  BLAST  Prediction  Download  Help  About  Links  PlantRegMap
(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G52830.1
Common NameATWRKY27, MXC20.5, WRKY27
Organism
Arabidopsis thaliana
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family WRKY
Protein Properties Length: 348aa    MW: 38721.5 Da    PI: 4.4731
Description WRKY DNA-binding protein 27
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G52830.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY98.44.6e-31165223259
                  --SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS
         WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 
                  +D ++WrKYGqK++kgs++pr+YYrC+s+ gC ++k+vers+ dp+++++tY+geH+h+
  AT5G52830.1 165 SDLWAWRKYGQKPIKGSPYPRNYYRCSSSkGCLARKQVERSNLDPNIFIVTYTGEHTHP 223
                  799************************998****************************8 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.20.25.804.6E-31151225IPR003657WRKY domain
SuperFamilySSF1182904.71E-27157225IPR003657WRKY domain
PROSITE profilePS5081133.212159225IPR003657WRKY domain
SMARTSM007742.5E-39164224IPR003657WRKY domain
PfamPF031064.5E-26166223IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0007263Biological Processnitric oxide mediated signal transduction
GO:0009739Biological Processresponse to gibberellin
GO:0042742Biological Processdefense response to bacterium
GO:0045892Biological Processnegative regulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0005515Molecular Functionprotein binding
GO:0043565Molecular Functionsequence-specific DNA binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000036anatomyleaf vascular system
PO:0000039anatomyshoot axis vascular system
PO:0000293anatomyguard cell
PO:0003011anatomyroot vascular system
PO:0005417anatomyphloem
PO:0006502anatomyfloral organ abscission zone
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0009066anatomyanther
PO:0020030anatomycotyledon
PO:0020100anatomyhypocotyl
PO:0025022anatomycollective leaf structure
PO:0025166anatomypapilla cell
PO:0025181anatomyroot elongation zone
PO:0025195anatomypollen tube cell
PO:0025281anatomypollen
PO:0001017developmental stageM germinated pollen stage
PO:0001054developmental stagevascular leaf senescent stage
PO:0001185developmental stageplant embryo globular stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 348 aa     Download sequence    Send to blast
MSSEDWDLFA VVRSCSSSVS TTNSCAGHED DIGNCKQQQD PPPPPLFQAS SSCNELQDSC  60
KPFLPVTTTT TTTWSPPPLL PPPKASSPSP NILLKQEQVL LESQDQKPPL SVRVFPPSTS  120
SSVFVFRGQR DQLLQQQSQP PLRSRKRKNQ QKRTICHVTQ ENLSSDLWAW RKYGQKPIKG  180
SPYPRNYYRC SSSKGCLARK QVERSNLDPN IFIVTYTGEH THPRPTHRNS LAGSTRNKSQ  240
PVNPVPKPDT SPLSDTVKEE IHLSPTTPLK GNDDVQETNG DEDMVGQEVN MEEEEEEEEV  300
EEDDEEEEDD DDVDDLLIPN LAVRDRDDLF FAGSFPSWSA GSAGDGGG
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5w3x_B3e-201662261880Disease resistance protein RRS1
5w3x_D3e-201662261880Disease resistance protein RRS1
Search in ModeBase
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible248306_at0.0
Expression AtlasAT5G52830-
AtGenExpressAT5G52830-
ATTED-IIAT5G52830-
Functional Description ? help Back to Top
Source Description
TAIREncodes a WRKY transcription factor WRKY27. Mutation in Arabidopsis WRKY27 results in delayed symptom development in response to the bacterial wilt pathogen Ralstonia solanacearum.
UniProtTranscription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250}.
Function -- GeneRIF ? help Back to Top
  1. Plants lacking a functional WRKY27 transcription factor showed delayed symptom development in response to Ralstonia solanacearum.
    [PMID: 18702671]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00561DAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G52830.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G37130(R), AT1G77760(R), AT5G65010(R)
Interaction ? help Back to Top
Source Intact With
BioGRIDAT1G10585
IntActSearch Q9FLX8
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G52830
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF4183100.0AF418310.1 Arabidopsis thaliana WRKY transcription factor 27 (WRKY27) mRNA, complete cds.
GenBankAK2304360.0AK230436.1 Arabidopsis thaliana mRNA for hypothetical protein, complete cds, clone: RAFL25-40-N05.
GenBankBT0264510.0BT026451.1 Arabidopsis thaliana At5g52830 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_568777.10.0WRKY DNA-binding protein 27
SwissprotQ9FLX80.0WRK27_ARATH; Probable WRKY transcription factor 27
TrEMBLQ0WKX30.0Q0WKX3_ARATH; At5g52830
STRINGAT5G52830.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM99982636
Representative plantOGRP1417875
Publications ? help Back to Top
  1. Eulgem T,Rushton PJ,Robatzek S,Somssich IE
    The WRKY superfamily of plant transcription factors.
    Trends Plant Sci., 2000. 5(5): p. 199-206
    [PMID:10785665]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Thilmony R,Underwood W,He SY
    Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7.
    Plant J., 2006. 46(1): p. 34-53
    [PMID:16553894]
  4. Zentella R, et al.
    Global analysis of della direct targets in early gibberellin signaling in Arabidopsis.
    Plant Cell, 2007. 19(10): p. 3037-57
    [PMID:17933900]
  5. Mukhtar MS,Deslandes L,Auriac MC,Marco Y,Somssich IE
    The Arabidopsis transcription factor WRKY27 influences wilt disease symptom development caused by Ralstonia solanacearum.
    Plant J., 2008. 56(6): p. 935-47
    [PMID:18702671]
  6. Wang Y, et al.
    Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
    Plant Physiol., 2008. 148(3): p. 1201-11
    [PMID:18775970]
  7. Chang IF, et al.
    Proteomic profiling of tandem affinity purified 14-3-3 protein complexes in Arabidopsis thaliana.
    Proteomics, 2009. 9(11): p. 2967-85
    [PMID:19452453]
  8. Ding Y, et al.
    Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
    BMC Plant Biol., 2013. 13: p. 229
    [PMID:24377444]
  9. Lumba S, et al.
    A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis.
    Dev. Cell, 2014. 29(3): p. 360-72
    [PMID:24823379]
  10. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]
  11. Mukhtar MS,Liu X,Somssich IE
    Elucidating the role of WRKY27 in male sterility in Arabidopsis.
    Plant Signal Behav, 2017. 12(9): p. e1363945
    [PMID:28816593]