PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G49450.1

Common Name

AtbZIP1, bZIP1, K7J8.13

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bZIP

Protein Properties

Length: 145aa MW: 16224.3 Da PI: 9.7147

Description

basic leucine-zipper 1

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G49450.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	bZIP_1	23.2	1.5e-07	15	81	4	63
XCHHHCHHHHHHHHHHHHHHHHHHHH.......HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH CS bZIP_1 4 lkrerrkqkNReAArrsRqRKkaeie.......eLeekvkeLeaeNkaLkkeleelkkevaklksev 63 k+ +rk +NRe+ArrsR++K++ +e Le+++ke ++ +a k++l++ + e a l+se+ AT5G49450.1 15 EKKRKRKLSNRESARRSRLKKQKLMEdtiheisSLERRIKENSERCRAVKQRLDSVETENAGLRSEK 81 5999******************966522222227999999999999999999999999999888875 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:1.20.5.170	1.7E-9	10	79	No hit	No description
SMART	SM00338	1.3E-8	12	76	IPR004827	Basic-leucine zipper domain
Pfam	PF07716	8.3E-6	14	55	IPR004827	Basic-leucine zipper domain
PROSITE profile	PS50217	9.002	14	77	IPR004827	Basic-leucine zipper domain
SuperFamily	SSF57959	1.59E-6	16	67	No hit	No description
CDD	cd14702	2.26E-13	17	68	No hit	No description
PROSITE pattern	PS00036	0	19	34	IPR004827	Basic-leucine zipper domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006521	Biological Process	regulation of cellular amino acid metabolic process
GO:0009267	Biological Process	cellular response to starvation
GO:0009617	Biological Process	response to bacterium
GO:0009651	Biological Process	response to salt stress
GO:0009901	Biological Process	anther dehiscence
GO:0010182	Biological Process	sugar mediated signaling pathway
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0071333	Biological Process	cellular response to glucose stimulus
GO:0005634	Cellular Component	nucleus
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding
GO:0046982	Molecular Function	protein heterodimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000293	anatomy	guard cell
PO:0009066	anatomy	anther
PO:0025195	anatomy	pollen tube cell
PO:0025281	anatomy	pollen
PO:0001016	developmental stage	L mature pollen stage
PO:0001017	developmental stage	M germinated pollen stage

Sequence ? help Back to Top
Protein Sequence Length: 145 aa Download sequence Send to blast
MANAEKTSSG SDIDEKKRKR KLSNRESARR SRLKKQKLME DTIHEISSLE RRIKENSERC 60 RAVKQRLDSV ETENAGLRSE KIWLSSYVSD LENMIATTSL TLTQSGGGDC VDDQNANAGI 120 AVGDCRRTPW KLSCGSLQPM ASFKT

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	14	21	EKKRKRKL
2	15	20	KKRKRK

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.26573	0.0	leaf\| root

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	248606_at	0.0
Expression Atlas	AT5G49450	-
AtGenExpress	AT5G49450	-
ATTED-II	AT5G49450	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Expressed in seeds during late stage of development. {ECO:0000269\|PubMed:18841482}.
Uniprot	TISSUE SPECIFICITY: Expressed in both shoots, including young leaves, stipulae and trichomes (except in cotyledons and hypocotyl), and roots, including vascular tissues (e.g. in both the phloem and the xylem). Present in seeds and pollen. Restricted to vasculatures and roots in the presence of sucrose or glucose. {ECO:0000269\|PubMed:18841482, ECO:0000269\|PubMed:20080816}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor that binds to the C-box-like motif (5'-TGCTGACGTCA-3') and G-box-like motif (5'-CCACGTGGCC-3'), ABRE elements, of gene promoters involved in sugar signaling. Activated by low energy stress both at transcriptional and post-transcriptional mechanisms. Promotes dark-induced senescence and participates in the transcriptional reprogramming of amino acid metabolism during the dark-induced starvation response (PubMed:20080816, PubMed:21278122). Transcription activator of the mannan synthase CSLA9. Recognizes and binds to DNA-specific sequence of CSLA9 promoter (PubMed:24243147). {ECO:0000269\|PubMed:20080816, ECO:0000269\|PubMed:21278122, ECO:0000269\|PubMed:24243147}.

Function -- GeneRIF ? help Back to Top
Data show that AtbZIP1 can bind ACGT-based motifs in vitro and that the binding characteristics appear to be affected by the heterodimerization between AtbZIP1 and the C-group AtbZIPs, including AtbZIP10 and AtbZIP63. [PMID: 20080816] bZIP1 and bZIP53 expression is enhanced during dark-induced starvation. Heterodimerization with members of the partially redundant C/S1 bZIP factor network reprograms primary metabolism in the starvation response. [PMID: 21278122] Data indicate that transcription factors ANAC041, bZIP1 and MYB46 directly regulate the expression of CSLA9. [PMID: 24243147] promoters of these transient targets are uniquely enriched with cis-regulatory motifs coinherited with bZIP1 binding sites, suggesting a recruitment role for bZIP1. [PMID: 24958886] Data show that the group S1 basic leucine zipper transcription factors bZIP1 and bZIP53 reprogram primary C- and N-metabolism. [PMID: 26276836]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G49450.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Reversibly repressed by glucose and mannose. Slowly induced by Pseudomonas syringae. Induced in roots upon cold and salt stress but then repressed in leaves. Promoted by low energy stress and dark-induced starvation. {ECO:0000269\|PubMed:18841482, ECO:0000269\|PubMed:20080816, ECO:0000269\|PubMed:21278122}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source	Upstream Regulator (A: Activate/R: Repress)
ATRM	AT2G01570 (A), AT2G46830 (R)

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT1G13600, AT1G59530, AT1G75390
IntAct	Search Q9FGX2

Phenotype -- Disruption Phenotype ? help Back to Top
Source	Description
UniProt	DISRUPTION PHENOTYPE: Reduced requirement for exogenous sugar for seedling growth and higher rates of true leaf development. {ECO:0000269\|PubMed:20080816}.

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G49450

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AB023034	0.0	AB023034.1 Arabidopsis thaliana genomic DNA, chromosome 5, TAC clone:K7J8.
GenBank	AF400618	0.0	AF400618.1 Arabidopsis thaliana transcription factor-like protein bZIP1 mRNA, complete cds.
GenBank	AY088207	0.0	AY088207.1 Arabidopsis thaliana clone 43004 mRNA, complete sequence.
GenBank	AY136307	0.0	AY136307.1 Arabidopsis thaliana putative protein (At5g49450) mRNA, complete cds.
GenBank	BT000400	0.0	BT000400.1 Arabidopsis thaliana putative protein (At5g49450) mRNA, complete cds.
GenBank	CP002688	0.0	CP002688.1 Arabidopsis thaliana chromosome 5 sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_199756.1	1e-102	basic leucine-zipper 1
Swissprot	Q9FGX2	1e-103	BZIP1_ARATH; Basic leucine zipper 1
TrEMBL	A0A178UPE4	1e-101	A0A178UPE4_ARATH; Uncharacterized protein
STRING	AT5G49450.1	1e-102	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM12581	22	31
Representative plant	OGRP13793	3	4

Link Out ? help Back to Top
Phytozome	AT5G49450.1
Entrez Gene	835005
iHOP	AT5G49450
wikigenes	AT5G49450

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Jakoby M, et al. bZIP transcription factors in Arabidopsis. Trends Plant Sci., 2002. 7(3): p. 106-11 [PMID:11906833] Dal Bosco C, et al. Inactivation of the chloroplast ATP synthase gamma subunit results in high non-photochemical fluorescence quenching and altered nuclear gene expression in Arabidopsis thaliana. J. Biol. Chem., 2004. 279(2): p. 1060-9 [PMID:14576160] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Jiao Y, et al. A genome-wide analysis of blue-light regulation of Arabidopsis transcription factor gene expression during seedling development. Plant Physiol., 2003. 133(4): p. 1480-93 [PMID:14605227] Satoh R,Fujita Y,Nakashima K,Shinozaki K,Yamaguchi-Shinozaki K A novel subgroup of bZIP proteins functions as transcriptional activators in hypoosmolarity-responsive expression of the ProDH gene in Arabidopsis. Plant Cell Physiol., 2004. 45(3): p. 309-17 [PMID:15047879] Wiese A,Elzinga N,Wobbes B,Smeekens S A conserved upstream open reading frame mediates sucrose-induced repression of translation. Plant Cell, 2004. 16(7): p. 1717-29 [PMID:15208401] Deppmann CD, et al. Dimerization specificity of all 67 B-ZIP motifs in Arabidopsis thaliana: a comparison to Homo sapiens B-ZIP motifs. Nucleic Acids Res., 2004. 32(11): p. 3435-45 [PMID:15226410] Guan Y,Nothnagel EA Binding of arabinogalactan proteins by Yariv phenylglycoside triggers wound-like responses in Arabidopsis cell cultures. Plant Physiol., 2004. 135(3): p. 1346-66 [PMID:15235117] Price J,Laxmi A,St Martin SK,Jang JC Global transcription profiling reveals multiple sugar signal transduction mechanisms in Arabidopsis. Plant Cell, 2004. 16(8): p. 2128-50 [PMID:15273295] Contento AL,Kim SJ,Bassham DC Transcriptome profiling of the response of Arabidopsis suspension culture cells to Suc starvation. Plant Physiol., 2004. 135(4): p. 2330-47 [PMID:15310832] Suzuki N, et al. Enhanced tolerance to environmental stress in transgenic plants expressing the transcriptional coactivator multiprotein bridging factor 1c. Plant Physiol., 2005. 139(3): p. 1313-22 [PMID:16244138] Vergnolle C, et al. The cold-induced early activation of phospholipase C and D pathways determines the response of two distinct clusters of genes in Arabidopsis cell suspensions. Plant Physiol., 2005. 139(3): p. 1217-33 [PMID:16258011] Truman W,de Zabala MT,Grant M Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance. Plant J., 2006. 46(1): p. 14-33 [PMID:16553893] Ehlert A, et al. Two-hybrid protein-protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors. Plant J., 2006. 46(5): p. 890-900 [PMID:16709202] Deppmann CD,Alvania RS,Taparowsky EJ Cross-species annotation of basic leucine zipper factor interactions: Insight into the evolution of closed interaction networks. Mol. Biol. Evol., 2006. 23(8): p. 1480-92 [PMID:16731568] Osuna D, et al. Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon-deprived Arabidopsis seedlings. Plant J., 2007. 49(3): p. 463-91 [PMID:17217462] de Torres-Zabala M, et al. Pseudomonas syringae pv. tomato hijacks the Arabidopsis abscisic acid signalling pathway to cause disease. EMBO J., 2007. 26(5): p. 1434-43 [PMID:17304219] Popescu SC, et al. Differential binding of calmodulin-related proteins to their targets revealed through high-density Arabidopsis protein microarrays. Proc. Natl. Acad. Sci. U.S.A., 2007. 104(11): p. 4730-5 [PMID:17360592] Hayden CA,Jorgensen RA Identification of novel conserved peptide uORF homology groups in Arabidopsis and rice reveals ancient eukaryotic origin of select groups and preferential association with transcription factor-encoding genes. BMC Biol., 2007. 5: p. 32 [PMID:17663791] Usadel B, et al. Global transcript levels respond to small changes of the carbon status during progressive exhaustion of carbohydrates in Arabidopsis rosettes. Plant Physiol., 2008. 146(4): p. 1834-61 [PMID:18305208] Gutiérrez RA, et al. Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1. Proc. Natl. Acad. Sci. U.S.A., 2008. 105(12): p. 4939-44 [PMID:18344319] Hou X, et al. Global identification of DELLA target genes during Arabidopsis flower development. Plant Physiol., 2008. 147(3): p. 1126-42 [PMID:18502975] Mentzen WI,Peng J,Ransom N,Nikolau BJ,Wurtele ES Articulation of three core metabolic processes in Arabidopsis: fatty acid biosynthesis, leucine catabolism and starch metabolism. BMC Plant Biol., 2008. 8: p. 76 [PMID:18616834] Wang Y, et al. Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol., 2008. 148(3): p. 1201-11 [PMID:18775970] Weltmeier F, et al. Expression patterns within the Arabidopsis C/S1 bZIP transcription factor network: availability of heterodimerization partners controls gene expression during stress response and development. Plant Mol. Biol., 2009. 69(1-2): p. 107-19 [PMID:18841482] Kang SG,Price J,Lin PC,Hong JC,Jang JC The arabidopsis bZIP1 transcription factor is involved in sugar signaling, protein networking, and DNA binding. Mol Plant, 2010. 3(2): p. 361-73 [PMID:20080816] Obertello M,Krouk G,Katari MS,Runko SJ,Coruzzi GM Modeling the global effect of the basic-leucine zipper transcription factor 1 (bZIP1) on nitrogen and light regulation in Arabidopsis. BMC Syst Biol, 2010. 4: p. 111 [PMID:20704717] Brady SM, et al. A stele-enriched gene regulatory network in the Arabidopsis root. Mol. Syst. Biol., 2011. 7: p. 459 [PMID:21245844] Dietrich K, et al. Heterodimers of the Arabidopsis transcription factors bZIP1 and bZIP53 reprogram amino acid metabolism during low energy stress. Plant Cell, 2011. 23(1): p. 381-95 [PMID:21278122] Arabidopsis Interactome Mapping Consortium Evidence for network evolution in an Arabidopsis interactome map. Science, 2011. 333(6042): p. 601-7 [PMID:21798944] Sun X, et al. The Arabidopsis AtbZIP1 transcription factor is a positive regulator of plant tolerance to salt, osmotic and drought stresses. J. Plant Res., 2012. 125(3): p. 429-38 [PMID:21938515] Renault H, et al. γ-Aminobutyric acid transaminase deficiency impairs central carbon metabolism and leads to cell wall defects during salt stress in Arabidopsis roots. Plant Cell Environ., 2013. 36(5): p. 1009-18 [PMID:23148892] Kim WC, et al. Transcription factors that directly regulate the expression of CSLA9 encoding mannan synthase in Arabidopsis thaliana. Plant Mol. Biol., 2014. 84(4-5): p. 577-87 [PMID:24243147] Para A, et al. Hit-and-run transcriptional control by bZIP1 mediates rapid nutrient signaling in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 2014. 111(28): p. 10371-6 [PMID:24958886] Jin J, et al. An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors. Mol. Biol. Evol., 2015. 32(7): p. 1767-73 [PMID:25750178] Mair A, et al. SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants. Elife, 2016. [PMID:26263501] Hartmann L, et al. Crosstalk between Two bZIP Signaling Pathways Orchestrates Salt-Induced Metabolic Reprogramming in Arabidopsis Roots. Plant Cell, 2015. 27(8): p. 2244-60 [PMID:26276836] Doidy J, et al. "Hit-and-Run" transcription: de novo transcription initiated by a transient bZIP1 "hit" persists after the "run". BMC Genomics, 2016. 17: p. 92 [PMID:26843062] Dash M, et al. Poplar PtabZIP1-like enhances lateral root formation and biomass growth under drought stress. Plant J., 2017. 89(4): p. 692-705 [PMID:27813246] Ezer D, et al. The G-Box Transcriptional Regulatory Code in Arabidopsis. Plant Physiol., 2017. 175(2): p. 628-640 [PMID:28864470] Lee DH,Park SJ,Ahn CS,Pai HS MRF Family Genes Are Involved in Translation Control, Especially under Energy-Deficient Conditions, and Their Expression and Functions Are Modulated by the TOR Signaling Pathway. Plant Cell, 2017. 29(11): p. 2895-2920 [PMID:29084871] Pedrotti L, et al. Snf1-RELATED KINASE1-Controlled C/S1-bZIP Signaling Activates Alternative Mitochondrial Metabolic Pathways to Ensure Plant Survival in Extended Darkness. Plant Cell, 2018. 30(2): p. 495-509 [PMID:29348240] Para A,Li Y,Coruzzi GM μChIP-Seq for Genome-Wide Mapping of In Vivo TF-DNA Interactions in Arabidopsis Root Protoplasts. Methods Mol. Biol., 2018. 1761: p. 249-261 [PMID:29525963]