PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G46830.1

Common Name

ATNIG1, BHLH28, EN40, MZA15.1, NIG1

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bHLH

Protein Properties

Length: 511aa MW: 57348 Da PI: 6.0145

Description

NACL-inducible gene 1

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G46830.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	37.1	5.8e-12	343	388	4	54
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 +h e+Er RR+++N++f Lr ++P+ K++K + Le Av YI++L AT5G46830.1 343 NHVEAERMRREKLNHRFYALRAVVPNV-----SKMDKTSLLEDAVCYINEL 388 799*********************6.....5*************998 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	2.1E-44	35	225	IPR025610	Transcription factor MYC/MYB N-terminal
CDD	cd00083	5.55E-13	338	389	No hit	No description
SuperFamily	SSF47459	3.14E-16	339	408	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	16.399	339	388	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	3.2E-9	343	388	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	7.6E-15	343	409	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	1.3E-13	345	394	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0042538	Biological Process	hyperosmotic salinity response
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005509	Molecular Function	calcium ion binding
GO:0046983	Molecular Function	protein dimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000037	anatomy	shoot apex
PO:0008019	anatomy	leaf lamina base
PO:0009006	anatomy	shoot system

Sequence ? help Back to Top
Protein Sequence Length: 511 aa Download sequence Send to blast
MINTDDNLLM IEALLTSDPS PPLLPANLSL ETTLPKRLHA VLNGTHEPWS YAIFWKPSYD 60 DFSGEAVLKW GDGVYTGGNE EKTRGRLRRK KTILSSPEEK ERRSNVIREL NLMISGEAFP 120 VVEDDVSDDD DVEVTDMEWF FLVSMTWSFG NGSGLAGKAF ASYNPVLVTG SDLIYGSGCD 180 RAKQGGDVGL QTILCIPSHN GVLELASTEE IRPNSDLFNR IRFLFGGSKY FSGAPNSNSE 240 LFPFQLESSC SSTVTGNPNP SPVYLQNRYN LNFSTSSSTL ARAPCGDVLS FGENVKQSFE 300 NRNPNTYSDQ IQNVVPHATV MLEKKKGKKR GRKPAHGRDK PLNHVEAERM RREKLNHRFY 360 ALRAVVPNVS KMDKTSLLED AVCYINELKS KAENVELEKH AIEIQFNELK EIAGQRNAIP 420 SVCKYEEKAS EMMKIEVKIM ESDDAMVRVE SRKDHHPGAR LMNALMDLEL EVNHASISVM 480 NDLMIQQANV KMGLRIYKQE ELRDLLMSKI S

Sequence ? help Back to Top

Protein Sequence Length: 511 aa Download sequence Send to blast

MINTDDNLLM IEALLTSDPS PPLLPANLSL ETTLPKRLHA VLNGTHEPWS YAIFWKPSYD  60
DFSGEAVLKW GDGVYTGGNE EKTRGRLRRK KTILSSPEEK ERRSNVIREL NLMISGEAFP  120
VVEDDVSDDD DVEVTDMEWF FLVSMTWSFG NGSGLAGKAF ASYNPVLVTG SDLIYGSGCD  180
RAKQGGDVGL QTILCIPSHN GVLELASTEE IRPNSDLFNR IRFLFGGSKY FSGAPNSNSE  240
LFPFQLESSC SSTVTGNPNP SPVYLQNRYN LNFSTSSSTL ARAPCGDVLS FGENVKQSFE  300
NRNPNTYSDQ IQNVVPHATV MLEKKKGKKR GRKPAHGRDK PLNHVEAERM RREKLNHRFY  360
ALRAVVPNVS KMDKTSLLED AVCYINELKS KAENVELEKH AIEIQFNELK EIAGQRNAIP  420
SVCKYEEKAS EMMKIEVKIM ESDDAMVRVE SRKDHHPGAR LMNALMDLEL EVNHASISVM  480
NDLMIQQANV KMGLRIYKQE ELRDLLMSKI S

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4rqw_A	1e-41	31	225	6	192	Transcription factor MYC3
4rqw_B	1e-41	31	225	6	192	Transcription factor MYC3
4rs9_A	1e-41	31	225	6	192	Transcription factor MYC3
4yz6_A	1e-41	31	225	6	192	Transcription factor MYC3
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Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	324	332	KKGKKRGRK

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	248867_at	0.0
Expression Atlas	AT5G46830	-
AtGenExpress	AT5G46830	-
ATTED-II	AT5G46830	-

Functional Description ? help Back to Top
Source	Description
TAIR	Calcium-binding transcription factor involved in salt stress signaling.

Function -- GeneRIF ? help Back to Top
MYC5, probably together with other, redundant transcription factors, may be activated by JA signaling to induce the expression of MYB21 and components required for male fertility. [MYC5] [PMID: 25627909] These findings define MYC5 as an important regulator that is essential for diverse jasmonic acid responses.[MYC5] [PMID: 29017003]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00547	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G46830.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By UV treatment. {ECO:0000269\|PubMed:12679534}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT5G60890, AT5G61420, AT1G18570, AT1G74080

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G46830

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AB022221	0.0	AB022221.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MSD23.
GenBank	AF252636	0.0	AF252636.1 Arabidopsis thaliana putative transcription factor bHLH28 mRNA, complete cds.
GenBank	CP002688	0.0	CP002688.1 Arabidopsis thaliana chromosome 5 sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_199495.1	0.0	calcium-binding transcription factor NIG1
Swissprot	Q9LUK7	0.0	BH028_ARATH; Transcription factor bHLH28
TrEMBL	C0SVS8	0.0	C0SVS8_ARATH; Uncharacterized protein At5g46830 (Fragment)
STRING	AT5G46830.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM13953	16	24
Representative plant	OGRP3733	12	24

Link Out ? help Back to Top
Phytozome	AT5G46830.1
Entrez Gene	834727
iHOP	AT5G46830
wikigenes	AT5G46830

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Day IS,Reddy VS,Shad Ali G,Reddy AS Analysis of EF-hand-containing proteins in Arabidopsis. Genome Biol., 2002. 3(10): p. RESEARCH0056 [PMID:12372144] Heim MA, et al. The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol. Biol. Evol., 2003. 20(5): p. 735-47 [PMID:12679534] Toledo-Ortiz G,Huq E,Quail PH The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell, 2003. 15(8): p. 1749-70 [PMID:12897250] Bailey PC, et al. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana. Plant Cell, 2003. 15(11): p. 2497-502 [PMID:14600211] Czechowski T,Bari RP,Stitt M,Scheible WR,Udvardi MK Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes. Plant J., 2004. 38(2): p. 366-79 [PMID:15078338] Lorenzo O,Chico JM,S JASMONATE-INSENSITIVE1 encodes a MYC transcription factor essential to discriminate between different jasmonate-regulated defense responses in Arabidopsis. Plant Cell, 2004. 16(7): p. 1938-50 [PMID:15208388] Kim J,Kim HY Functional analysis of a calcium-binding transcription factor involved in plant salt stress signaling. FEBS Lett., 2006. 580(22): p. 5251-6 [PMID:16962584] Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Tominaga-Wada R,Iwata M,Nukumizu Y,Wada T Analysis of IIId, IIIe and IVa group basic-helix-loop-helix proteins expressed in Arabidopsis root epidermis. Plant Sci., 2011. 181(4): p. 471-8 [PMID:21889054] Frerigmann H,Berger B,Gigolashvili T bHLH05 is an interaction partner of MYB51 and a novel regulator of glucosinolate biosynthesis in Arabidopsis. Plant Physiol., 2014. 166(1): p. 349-69 [PMID:25049362] Figueroa P,Browse J Male sterility in Arabidopsis induced by overexpression of a MYC5-SRDX chimeric repressor. Plant J., 2015. 81(6): p. 849-60 [PMID:25627909] Qi T,Huang H,Song S,Xie D Regulation of Jasmonate-Mediated Stamen Development and Seed Production by a bHLH-MYB Complex in Arabidopsis. Plant Cell, 2015. 27(6): p. 1620-33 [PMID:26002869] Chen X,Huang H,Qi T,Liu B,Song S New perspective of the bHLH-MYB complex in jasmonate-regulated plant fertility in arabidopsis. Plant Signal Behav, 2016. 11(2): p. e1135280 [PMID:26829586] Ezer D, et al. The G-Box Transcriptional Regulatory Code in Arabidopsis. Plant Physiol., 2017. 175(2): p. 628-640 [PMID:28864470] Song S, et al. MYC5 is Involved in Jasmonate-Regulated Plant Growth, Leaf Senescence and Defense Responses. Plant Cell Physiol., 2017. 58(10): p. 1752-1763 [PMID:29017003] Min JH, et al. Arabidopsis Basic Helix-Loop-Helix 34 (bHLH34) Is Involved in Glucose Signaling through Binding to a GAGA Cis-Element. Front Plant Sci, 2017. 8: p. 2100 [PMID:29321786]