PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G45050.2

Common Name

ATWRKY16, TTR1, WRKY16

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

WRKY

Protein Properties

Length: 1344aa MW: 152643 Da PI: 6.3271

Description

Disease resistance protein (TIR-NBS-LRR class)

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G45050.2	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	89.4	3e-28	1152	1210	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 +D + WrKYGqK++k+s++prsYYrC+s+ gC ++k+vers++dp+v +itY +eHnh+ AT5G45050.2 1152 SDLWVWRKYGQKPIKSSPYPRSYYRCASSkGCFARKQVERSRTDPNVSVITYISEHNHP 1210 699*********************9988**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF52200	2.62E-6	8	98	IPR000157	Toll/interleukin-1 receptor homology (TIR) domain
SuperFamily	SSF52540	3.33E-49	130	390	IPR027417	P-loop containing nucleoside triphosphate hydrolase
Pfam	PF00931	2.6E-27	154	399	IPR002182	NB-ARC
Gene3D	G3DSA:3.40.50.300	3.4E-10	161	285	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PRINTS	PR00364	6.6E-23	165	180	No hit	No description
PRINTS	PR00364	6.6E-23	237	251	No hit	No description
PRINTS	PR00364	6.6E-23	330	344	No hit	No description
SuperFamily	SSF46785	1.82E-12	370	467	IPR011991	Winged helix-turn-helix DNA-binding domain
Gene3D	G3DSA:3.40.50.300	6.5E-5	418	469	IPR027417	P-loop containing nucleoside triphosphate hydrolase
SuperFamily	SSF52058	1.09E-29	522	792	IPR032675	Leucine-rich repeat domain, L domain-like
Gene3D	G3DSA:3.80.10.10	4.2E-24	534	783	IPR032675	Leucine-rich repeat domain, L domain-like
Pfam	PF07725	9.0E-9	570	589	IPR011713	Leucine-rich repeat 3
PRINTS	PR00364	6.6E-23	716	732	No hit	No description
Gene3D	G3DSA:2.20.25.80	3.0E-27	1138	1212	IPR003657	WRKY domain
SuperFamily	SSF118290	1.44E-24	1149	1212	IPR003657	WRKY domain
SMART	SM00774	3.5E-34	1151	1211	IPR003657	WRKY domain
PROSITE profile	PS50811	30.745	1152	1212	IPR003657	WRKY domain
Pfam	PF03106	4.4E-23	1153	1210	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0006952	Biological Process	defense response
GO:0007165	Biological Process	signal transduction
GO:0005634	Cellular Component	nucleus
GO:0005774	Cellular Component	vacuolar membrane
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0005524	Molecular Function	ATP binding
GO:0043531	Molecular Function	ADP binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000005	anatomy	cultured plant cell
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 1344 aa Download sequence Send to blast
MTESEQIVYI SCIEEVRYSF VSHLSKALQR KGVNDVFIDS DDSLSNESQS MVERARVSVM 60 ILPGNRTVSL DKLVKVLDCQ KNKDQVVVPV LYGVRSSETE WLSALDSKGF SSVHHSRKEC 120 SDSQLVKETV RDVYEKLFYM ERIGIYSKLL EIEKMINKQP LDIRCVGIWG MPGIGKTTLA 180 KAVFDQMSGE FDAHCFIEDY TKAIQEKGVY CLLEEQFLKE NAGASGTVTK LSLLRDRLNN 240 KRVLVVLDDV RSPLVVESFL GGFDWFGPKS LIIITSKDKS VFRLCRVNQI YEVQGLNEKE 300 ALQLFSLCAS IDDMAEQNLH EVSMKVIKYA NGHPLALNLY GRELMGKKRP PEMEIAFLKL 360 KECPPAIFVD AIKSSYDTLN DREKNIFLDI ACFFQGENVD YVMQLLEGCG FFPHVGIDVL 420 VEKSLVTISE NRVRMHNLIQ DVGRQIINRE TRQTKRRSRL WEPCSIKYLL EDKEQNENEE 480 QKTTFERAQV PEEIEGMFLD TSNLSFDIKH VAFDNMLNLR LFKIYSSNPE VHHVNNFLKG 540 SLSSLPNVLR LLHWENYPLQ FLPQNFDPIH LVEINMPYSQ LKKLWGGTKD LEMLKTIRLC 600 HSQQLVDIDD LLKAQNLEVV DLQGCTRLQS FPATGQLLHL RVVNLSGCTE IKSFPEIPPN 660 IETLNLQGTG VSNLEQSDLK PLTSLMKIST SYQNPGKLSC LELNDCSRLR SLPNMVNLEL 720 LKALDLSGCS ELETIQGFPR NLKELYLVGT AVRQVPQLPQ SLEFFNAHGC VSLKSIRLDF 780 KKLPVHYTFS NCFDLSPQVV NDFLVQAMAN VIAKHIPRER HVTGFSQKTV QRSSRDSQQE 840 LNKTLAFSFC APSHANQNSK LDLQPGSSSM TRLDPSWRNT LVGFAMLVQV AFSEGYCDDT 900 DFGISCVCKW KNKEGHSHRR EINLHCWALG KAVERDHTFV FFDVNMRPDT DEGNDPDIWA 960 DLVVFEFFPV NKQRKPLNDS CTVTRCGVRL ITAVNCNTSI ENISPVLSLD PMEVSGNEDE 1020 EVLRVRYAGL QEIYKALFLY IAGLFNDEDV GLVAPLIANI IDMDVSYGLK VLAYRSLIRV 1080 SSNGEIVMHY LLRQMGKEIL HTESKKTDKL VDNIQSSMIA TKEIEITRSK SRRKNNKEKR 1140 VVCVVDRGSR SSDLWVWRKY GQKPIKSSPY PRSYYRCASS KGCFARKQVE RSRTDPNVSV 1200 ITYISEHNHP FPTLRNTLAG STRSSSSKCS DVTTSASSTV SQDKEGPDKS HLPSSPASPP 1260 YAAMVVKEED MEQWDNMEFD VDVEEDTFIP ELFPEDTFAD MDKLEENSQT MFLSRRSSGG 1320 NMEAQGKNSS DDREVNLPSK ILNR

Sequence ? help Back to Top

Protein Sequence Length: 1344 aa Download sequence Send to blast

MTESEQIVYI SCIEEVRYSF VSHLSKALQR KGVNDVFIDS DDSLSNESQS MVERARVSVM  60
ILPGNRTVSL DKLVKVLDCQ KNKDQVVVPV LYGVRSSETE WLSALDSKGF SSVHHSRKEC  120
SDSQLVKETV RDVYEKLFYM ERIGIYSKLL EIEKMINKQP LDIRCVGIWG MPGIGKTTLA  180
KAVFDQMSGE FDAHCFIEDY TKAIQEKGVY CLLEEQFLKE NAGASGTVTK LSLLRDRLNN  240
KRVLVVLDDV RSPLVVESFL GGFDWFGPKS LIIITSKDKS VFRLCRVNQI YEVQGLNEKE  300
ALQLFSLCAS IDDMAEQNLH EVSMKVIKYA NGHPLALNLY GRELMGKKRP PEMEIAFLKL  360
KECPPAIFVD AIKSSYDTLN DREKNIFLDI ACFFQGENVD YVMQLLEGCG FFPHVGIDVL  420
VEKSLVTISE NRVRMHNLIQ DVGRQIINRE TRQTKRRSRL WEPCSIKYLL EDKEQNENEE  480
QKTTFERAQV PEEIEGMFLD TSNLSFDIKH VAFDNMLNLR LFKIYSSNPE VHHVNNFLKG  540
SLSSLPNVLR LLHWENYPLQ FLPQNFDPIH LVEINMPYSQ LKKLWGGTKD LEMLKTIRLC  600
HSQQLVDIDD LLKAQNLEVV DLQGCTRLQS FPATGQLLHL RVVNLSGCTE IKSFPEIPPN  660
IETLNLQGTG VSNLEQSDLK PLTSLMKIST SYQNPGKLSC LELNDCSRLR SLPNMVNLEL  720
LKALDLSGCS ELETIQGFPR NLKELYLVGT AVRQVPQLPQ SLEFFNAHGC VSLKSIRLDF  780
KKLPVHYTFS NCFDLSPQVV NDFLVQAMAN VIAKHIPRER HVTGFSQKTV QRSSRDSQQE  840
LNKTLAFSFC APSHANQNSK LDLQPGSSSM TRLDPSWRNT LVGFAMLVQV AFSEGYCDDT  900
DFGISCVCKW KNKEGHSHRR EINLHCWALG KAVERDHTFV FFDVNMRPDT DEGNDPDIWA  960
DLVVFEFFPV NKQRKPLNDS CTVTRCGVRL ITAVNCNTSI ENISPVLSLD PMEVSGNEDE  1020
EVLRVRYAGL QEIYKALFLY IAGLFNDEDV GLVAPLIANI IDMDVSYGLK VLAYRSLIRV  1080
SSNGEIVMHY LLRQMGKEIL HTESKKTDKL VDNIQSSMIA TKEIEITRSK SRRKNNKEKR  1140
VVCVVDRGSR SSDLWVWRKY GQKPIKSSPY PRSYYRCASS KGCFARKQVE RSRTDPNVSV  1200
ITYISEHNHP FPTLRNTLAG STRSSSSKCS DVTTSASSTV SQDKEGPDKS HLPSSPASPP  1260
YAAMVVKEED MEQWDNMEFD VDVEEDTFIP ELFPEDTFAD MDKLEENSQT MFLSRRSSGG  1320
NMEAQGKNSS DDREVNLPSK ILNR

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4c6s_A	4e-41	5	144	5	150	PROBABLE WRKY TRANSCRIPTION FACTOR 52
4c6t_A	4e-41	2	144	3	151	PROBABLE WRKY TRANSCRIPTION FACTOR 52
4c6t_C	4e-41	2	144	3	151	PROBABLE WRKY TRANSCRIPTION FACTOR 52
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Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.21909	0.0	flower\| leaf\| root\| seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	30694674	0.0
Genevisible	248973_at	0.0
Expression Atlas	AT5G45050	-
AtGenExpress	AT5G45050	-
ATTED-II	AT5G45050	-

Functional Description ? help Back to Top
Source	Description
TAIR	Encodes a member of WRKY Transcription Factor (Group II-e) that confers resistance to tobacco ringspot nepovirus.
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Acts also as a disease resistance protein that specifically recognizes the AvrRps4 type III effector avirulence protein from P.syringae. Heterodimerization with RPS4B is required to form a functional complex to recognize AvrRps4 and to mediate the hypersensitive response (PubMed:25744164). {ECO:0000250\|UniProtKB:P0DKH5, ECO:0000269\|PubMed:25744164}.

Function -- GeneRIF ? help Back to Top
An R gene pair that resembles and is linked to RRS1/RPS4, designated as RRS1B/RPS4B, confers recognition of AvrRps4 from Pseudomonas syringae. [RRS1B] [PMID: 25744164]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G45050.2

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G45050

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AY045888	0.0	AY045888.2 Arabidopsis thaliana At5g45050 mRNA sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_199318.2	0.0	Disease resistance protein (TIR-NBS-LRR class)
Swissprot	Q9FL92	0.0	RRS1B_ARATH; Disease resistance protein RRS1B
TrEMBL	F4KBS5	0.0	F4KBS5_ARATH; Disease resistance protein (TIR-NBS-LRR class)
STRING	AT5G45050.1	0.0	(Arabidopsis thaliana)

Link Out ? help Back to Top
Phytozome	AT5G45050.2
Entrez Gene	834536
iHOP	AT5G45050
wikigenes	AT5G45050

Publications ? help Back to Top
Eulgem T,Rushton PJ,Robatzek S,Somssich IE The WRKY superfamily of plant transcription factors. Trends Plant Sci., 2000. 5(5): p. 199-206 [PMID:10785665] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Meyers BC,Kozik A,Griego A,Kuang H,Michelmore RW Genome-wide analysis of NBS-LRR-encoding genes in Arabidopsis. Plant Cell, 2003. 15(4): p. 809-34 [PMID:12671079] Jaquinod M, et al. A proteomics dissection of Arabidopsis thaliana vacuoles isolated from cell culture. Mol. Cell Proteomics, 2007. 6(3): p. 394-412 [PMID:17151019] Tan X, et al. Global expression analysis of nucleotide binding site-leucine rich repeat-encoding and related genes in Arabidopsis. BMC Plant Biol., 2007. 7: p. 56 [PMID:17956627] Chang IF, et al. Proteomic profiling of tandem affinity purified 14-3-3 protein complexes in Arabidopsis thaliana. Proteomics, 2009. 9(11): p. 2967-85 [PMID:19452453] Saucet SB, et al. Two linked pairs of Arabidopsis TNL resistance genes independently confer recognition of bacterial effector AvrRps4. Nat Commun, 2015. 6: p. 6338 [PMID:25744164] Lee JM,Hartman GL,Domier LL,Bent AF Identification and map location of TTR1, a single locus in Arabidopsis thaliana that confers tolerance to tobacco ringspot nepovirus. Mol. Plant Microbe Interact., 1996. 9(8): p. 729-35 [PMID:8870272] Speulman E,Bouchez D,Holub EB,Beynon JL Disease resistance gene homologs correlate with disease resistance loci of Arabidopsis thaliana. Plant J., 1998. 14(4): p. 467-74 [PMID:9670562]