PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G24590.2

Common Name

ANAC091, K18P6.12, NAC091, TIP

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

NAC

Protein Properties

Length: 451aa MW: 50890.7 Da PI: 5.8496

Description

TCV-interacting protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G24590.2	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	NAM	162.7	1.3e-50	13	141	1	128
NAM 1 lppGfrFhPtdeelvveyLkkkvegkkleleevikevdiykvePwdLp..kkvkaeekewyfFskrdkkyatgkrknratksgyWkatgkdkevlskk 96 lp+GfrF+Ptdeelv +yL+ k++g+++++ +vi+e+di+k+ePwdLp + vk++++ew fF++ d+ky++g+r nrat +gyWkatgkd++++s k AT5G24590.2 13 LPVGFRFSPTDEELVRYYLRLKINGHDNDV-RVIREIDICKWEPWDLPdfSVVKTTDSEWLFFCPLDRKYPSGSRMNRATVAGYWKATGKDRKIKSGK 109 79***********************999.99***********6447888999**********************************99 PP NAM 97 gelvglkktLvfykgrapkgektdWvmheyrl 128 ++ +g+k+tLvfy+grapkg++t W+mheyr+ AT5G24590.2 110 TKIIGVKRTLVFYTGRAPKGTRTCWIMHEYRA 141 99**************************96 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF101941	8.37E-58	5	164	IPR003441	NAC domain
PROSITE profile	PS51005	54.705	13	164	IPR003441	NAC domain
Pfam	PF02365	7.6E-25	14	140	IPR003441	NAC domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0002230	Biological Process	positive regulation of defense response to virus by host
GO:0002237	Biological Process	response to molecule of bacterial origin
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0007275	Biological Process	multicellular organism development
GO:0005634	Cellular Component	nucleus
GO:0012505	Cellular Component	endomembrane system
GO:0016021	Cellular Component	integral component of membrane
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0003713	Molecular Function	transcription coactivator activity
GO:0005515	Molecular Function	protein binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000084	anatomy	plant sperm cell
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025195	anatomy	pollen tube cell
PO:0025281	anatomy	pollen
PO:0001017	developmental stage	M germinated pollen stage
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 451 aa Download sequence Send to blast
MKEDMEVLSL ASLPVGFRFS PTDEELVRYY LRLKINGHDN DVRVIREIDI CKWEPWDLPD 60 FSVVKTTDSE WLFFCPLDRK YPSGSRMNRA TVAGYWKATG KDRKIKSGKT KIIGVKRTLV 120 FYTGRAPKGT RTCWIMHEYR ATEKDLDGTK SGQNPFVVCK LFKKQDIVNG AAEPEESKSC 180 EVEPAVSSPT VVDEVEMSEV SPVFPKTEET NPCDVAESSL VIPSECRSGY SVPEVTTTGL 240 DDIDWLSFME FDSPKLFSPL HSQVQSELGS SFNGLQSESS ELFKNHNEDY IQTQYGTNDA 300 DEYMSKFLDS FLDIPYEPEQ IPYEPQNLSS CNKINDESKR GIKIRARRAQ APGCAEQFVM 360 QGDASRRLRL QVNLNSHKSE TDSTQLQFIK KEVKDTTTET MTKGCGNFTR SKSRTSFIFK 420 KIAAMGCSYR GLFRVGVVAV VCVMSVCSLV A

Sequence ? help Back to Top

Protein Sequence Length: 451 aa Download sequence Send to blast

MKEDMEVLSL ASLPVGFRFS PTDEELVRYY LRLKINGHDN DVRVIREIDI CKWEPWDLPD  60
FSVVKTTDSE WLFFCPLDRK YPSGSRMNRA TVAGYWKATG KDRKIKSGKT KIIGVKRTLV  120
FYTGRAPKGT RTCWIMHEYR ATEKDLDGTK SGQNPFVVCK LFKKQDIVNG AAEPEESKSC  180
EVEPAVSSPT VVDEVEMSEV SPVFPKTEET NPCDVAESSL VIPSECRSGY SVPEVTTTGL  240
DDIDWLSFME FDSPKLFSPL HSQVQSELGS SFNGLQSESS ELFKNHNEDY IQTQYGTNDA  300
DEYMSKFLDS FLDIPYEPEQ IPYEPQNLSS CNKINDESKR GIKIRARRAQ APGCAEQFVM  360
QGDASRRLRL QVNLNSHKSE TDSTQLQFIK KEVKDTTTET MTKGCGNFTR SKSRTSFIFK  420
KIAAMGCSYR GLFRVGVVAV VCVMSVCSLV A

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1ut4_A	6e-47	3	165	7	166	NO APICAL MERISTEM PROTEIN
1ut4_B	6e-47	3	165	7	166	NO APICAL MERISTEM PROTEIN
1ut7_A	6e-47	3	165	7	166	NO APICAL MERISTEM PROTEIN
1ut7_B	6e-47	3	165	7	166	NO APICAL MERISTEM PROTEIN
3swm_A	8e-47	3	165	10	169	NAC domain-containing protein 19
3swm_B	8e-47	3	165	10	169	NAC domain-containing protein 19
3swm_C	8e-47	3	165	10	169	NAC domain-containing protein 19
3swm_D	8e-47	3	165	10	169	NAC domain-containing protein 19
3swp_A	8e-47	3	165	10	169	NAC domain-containing protein 19
3swp_B	8e-47	3	165	10	169	NAC domain-containing protein 19
3swp_C	8e-47	3	165	10	169	NAC domain-containing protein 19
3swp_D	8e-47	3	165	10	169	NAC domain-containing protein 19
4dul_A	6e-47	3	165	7	166	NAC domain-containing protein 19
4dul_B	6e-47	3	165	7	166	NAC domain-containing protein 19
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.65025	0.0	flower\| leaf\| root\| seed\| silique

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	9408600	0.0
Genevisible	249746_at	0.0
Expression Atlas	AT5G24590	-
AtGenExpress	AT5G24590	-
ATTED-II	AT5G24590	-

Functional Description ? help Back to Top
Source	Description
TAIR	Member of NAc protein family. Interacts with turnip crinkle virus (TCV) capsid protein. Transcription factor involved in regulating the defense response of Arabidopsis to TCV.
UniProt	Transcription activator essential for the anti-viral defense called virus basal resistance response pathway (PubMed:11041886, PubMed:15629774, PubMed:18785827, PubMed:24418554). Not involved in HRT-mediated hypersensitive response (HR) and resistance to TCV (PubMed:18785827). Binds DNA non specifically (PubMed:15629774). Activated by proteolytic cleavage through regulated intramembrane proteolysis (RIP) (By similarity). {ECO:0000250\|UniProtKB:Q9SCK6, ECO:0000269\|PubMed:11041886, ECO:0000269\|PubMed:15629774, ECO:0000269\|PubMed:18785827, ECO:0000269\|PubMed:24418554}.; FUNCTION: (Microbial infection) Compromised function in defense response pathway when interacting with the invading viral capsid protein (CP) of turnip crinkle virus (TCV) due to abnormal subcellular localization. {ECO:0000269\|PubMed:11041886, ECO:0000269\|PubMed:15629774}.

Function -- GeneRIF ? help Back to Top
Results show that loss of TIP does not alter HR or resistance to TCV, but resulted in increased replication of TCV and Cucumber mosaic virus, suggesting that TIP may play a role in basal resistance but is not required for HRT-mediated signaling. [PMID: 18785827] Turnip crinkle virus coat protein inhibits the basal immune response in Arabidopsis by binding to the TIP protein. [PMID: 24418554]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G24590.2

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by bacterial pathogens type III effector proteins (TTEs). {ECO:0000269\|PubMed:16553893}.; INDUCTION: (Microbial infection) Accumulates 2 days post infection with turnip crinkle virus (TCV) (PubMed:24418554). {ECO:0000269\|PubMed:24418554}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Phenotype -- Disruption Phenotype ? help Back to Top
Source	Description
UniProt	DISRUPTION PHENOTYPE: Increased replication of turnip crinkle virus (TCV) and cucumber mosaic virus (CMV) (PubMed:18785827, PubMed:24418554). Normal HRT-mediated hypersensitive response (HR) and resistance to TCV (PubMed:18785827). Delayed flower development in antisense asTIP plants (PubMed:24418554). {ECO:0000269\|PubMed:18785827, ECO:0000269\|PubMed:24418554}.

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G24590

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF281062	0.0	AF281062.1 Arabidopsis thaliana TIP mRNA, complete cds.
GenBank	BT002012	0.0	BT002012.1 Arabidopsis thaliana NAC2-like protein (At5g24590) mRNA, complete cds.
GenBank	BT009683	0.0	BT009683.1 Arabidopsis thaliana At5g24590 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_197847.3	0.0	TCV-interacting protein
Swissprot	Q9LKG8	0.0	NAC91_ARATH; NAC domain-containing protein 91
TrEMBL	A0A178UKU0	0.0	A0A178UKU0_ARATH; TIP
STRING	AT5G24590.2	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM2416	28	73
Representative plant	OGRP9409	6	12

Link Out ? help Back to Top
Phytozome	AT5G24590.2
Entrez Gene	832530
iHOP	AT5G24590
wikigenes	AT5G24590

Publications ? help Back to Top
Ren T,Qu F,Morris TJ HRT gene function requires interaction between a NAC protein and viral capsid protein to confer resistance to turnip crinkle virus. Plant Cell, 2000. 12(10): p. 1917-26 [PMID:11041886] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Ooka H, et al. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana. DNA Res., 2003. 10(6): p. 239-47 [PMID:15029955] Ernst HA,Olsen AN,Larsen S,Lo Leggio L Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors. EMBO Rep., 2004. 5(3): p. 297-303 [PMID:15083810] Ren T,Qu F,Morris TJ The nuclear localization of the Arabidopsis transcription factor TIP is blocked by its interaction with the coat protein of Turnip crinkle virus. Virology, 2005. 331(2): p. 316-24 [PMID:15629774] Lee BH,Henderson DA,Zhu JK The Arabidopsis cold-responsive transcriptome and its regulation by ICE1. Plant Cell, 2005. 17(11): p. 3155-75 [PMID:16214899] Suzuki N, et al. Enhanced tolerance to environmental stress in transgenic plants expressing the transcriptional coactivator multiprotein bridging factor 1c. Plant Physiol., 2005. 139(3): p. 1313-22 [PMID:16244138] Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Truman W,de Zabala MT,Grant M Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance. Plant J., 2006. 46(1): p. 14-33 [PMID:16553893] Ma S,Bohnert HJ Integration of Arabidopsis thaliana stress-related transcript profiles, promoter structures, and cell-specific expression. Genome Biol., 2007. 8(4): p. R49 [PMID:17408486] Huang D,Wu W,Abrams SR,Cutler AJ The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors. J. Exp. Bot., 2008. 59(11): p. 2991-3007 [PMID:18552355] Wang Y, et al. Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol., 2008. 148(3): p. 1201-11 [PMID:18775970] Jeong RD,Chandra-Shekara AC,Kachroo A,Klessig DF,Kachroo P HRT-mediated hypersensitive response and resistance to Turnip crinkle virus in Arabidopsis does not require the function of TIP, the presumed guardee protein. Mol. Plant Microbe Interact., 2008. 21(10): p. 1316-24 [PMID:18785827] Kim MJ,Shin R,Schachtman DP A nuclear factor regulates abscisic acid responses in Arabidopsis. Plant Physiol., 2009. 151(3): p. 1433-45 [PMID:19759343] Gaudinier A, et al. Enhanced Y1H assays for Arabidopsis. Nat. Methods, 2011. 8(12): p. 1053-5 [PMID:22037706] Donze T,Qu F,Twigg P,Morris TJ Turnip crinkle virus coat protein inhibits the basal immune response to virus invasion in Arabidopsis by binding to the NAC transcription factor TIP. Virology, 2014. 449: p. 207-14 [PMID:24418554] Ben Daniel BH, et al. Identification of novel transcriptional regulators of Zat12 using comprehensive yeast one-hybrid screens. Physiol Plant, 2016. 157(4): p. 422-41 [PMID:26923089] Li Y, et al. The interaction between Turnip crinkle virus p38 and Cucumber mosaic virus 2b and its critical domains. Virus Res., 2016. 222: p. 94-105 [PMID:27288723]