PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT5G24110.1

Common Name

ATWRKY30, MLE8.3, MZF18.18, WRKY30

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

WRKY

Protein Properties

Length: 303aa MW: 33985.3 Da PI: 6.5735

Description

WRKY DNA-binding protein 30

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT5G24110.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	85.8	3.9e-27	112	173	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 ldDg++WrKYGqK++ g++fpr+YYrCt++ gC+++k+v+rs+e++ ++ei Y+g H ++ AT5G24110.1 112 LDDGFSWRKYGQKDILGAKFPRGYYRCTYRksqGCEATKQVQRSDENQMLLEISYRGIHSCS 173 59*************************98889***********************997 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	21.023	107	171	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	1.4E-25	110	174	IPR003657	WRKY domain
SuperFamily	SSF118290	1.24E-23	111	174	IPR003657	WRKY domain
SMART	SM00774	1.1E-38	112	174	IPR003657	WRKY domain
Pfam	PF03106	5.3E-25	113	172	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009751	Biological Process	response to salicylic acid
GO:0010193	Biological Process	response to ozone
GO:0042542	Biological Process	response to hydrogen peroxide
GO:0005634	Cellular Component	nucleus
GO:0005737	Cellular Component	cytoplasm
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0043565	Molecular Function	sequence-specific DNA binding
GO:0044212	Molecular Function	transcription regulatory region DNA binding

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000293	anatomy	guard cell
PO:0009029	anatomy	stamen
PO:0009047	anatomy	stem
PO:0025034	anatomy	leaf
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 303 aa Download sequence Send to blast
MEKNHSSGEW EKMKNEINEL MIEGRDYAHQ FGSASSQETR EHLAKKILQS YHKSLTIMNY 60 SGELDQVSQG GGSPKSDDSD QEPLVIKSSK KSMPRWSSKV RIAPGAGVDR TLDDGFSWRK 120 YGQKDILGAK FPRGYYRCTY RKSQGCEATK QVQRSDENQM LLEISYRGIH SCSQAANVGT 180 TMPIQNLEPN QTQEHGNLDM VKESVDNYNH QAHLHHNLHY PLSSTPNLEN NNAYMLQMRD 240 QNIEYFGSTS FSSDLGTSIN YNFPASGSAS HSASNSPSTV PLESPFESYD PNHPYGGFGG 300 FYS

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5w3x_B	6e-20	112	170	14	74	Disease resistance protein RRS1
5w3x_D	6e-20	112	170	14	74	Disease resistance protein RRS1
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Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.9177	0.0	seed

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	30689279	0.0
Genevisible	249770_at	0.0
Expression Atlas	AT5G24110	-
AtGenExpress	AT5G24110	-
ATTED-II	AT5G24110	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Accumulates during developmental leaf senescence. {ECO:0000269\|PubMed:22268143}.

Functional Description ? help Back to Top
Source	Description
TAIR	member of WRKY Transcription Factor; Group III
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. {ECO:0000250\|UniProtKB:Q9SUP6}.

Function -- GeneRIF ? help Back to Top
results presented here show that AtWRKY30 is responsive to several stress conditions either from abiotic or biotic origin, suggesting that AtWRKY30 could have a role in the activation of defence responses at early stages of Arabidopsis growth [PMID: 23794142]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00525	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT5G24110.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid (SA). {ECO:0000269\|PubMed:22268143}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT5G24110

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF404858	0.0	AF404858.1 Arabidopsis thaliana WRKY transcription factor 30 (WRKY30) mRNA, complete cds.
GenBank	AK117885	0.0	AK117885.1 Arabidopsis thaliana At5g24110 mRNA for unknown protein, complete cds, clone: RAFL19-06-C08.
GenBank	BT005295	0.0	BT005295.1 Arabidopsis thaliana At5g24110 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_568439.1	0.0	WRKY DNA-binding protein 30
Swissprot	Q9FL62	0.0	WRK30_ARATH; Probable WRKY transcription factor 30
TrEMBL	A0A178UIT8	0.0	A0A178UIT8_ARATH; WRKY30
STRING	AT5G24110.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM12595	19	28
Representative plant	OGRP14	17	875

Link Out ? help Back to Top
Phytozome	AT5G24110.1
Entrez Gene	832476
iHOP	AT5G24110
wikigenes	AT5G24110

Publications ? help Back to Top
Eulgem T,Rushton PJ,Robatzek S,Somssich IE The WRKY superfamily of plant transcription factors. Trends Plant Sci., 2000. 5(5): p. 199-206 [PMID:10785665] Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Seki M, et al. Functional annotation of a full-length Arabidopsis cDNA collection. Science, 2002. 296(5565): p. 141-5 [PMID:11910074] de Torres M,Sanchez P,Fernandez-Delmond I,Grant M Expression profiling of the host response to bacterial infection: the transition from basal to induced defence responses in RPM1-mediated resistance. Plant J., 2003. 33(4): p. 665-76 [PMID:12609040] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Vanderauwera S, et al. Genome-wide analysis of hydrogen peroxide-regulated gene expression in Arabidopsis reveals a high light-induced transcriptional cluster involved in anthocyanin biosynthesis. Plant Physiol., 2005. 139(2): p. 806-21 [PMID:16183842] Taki N, et al. 12-oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis. Plant Physiol., 2005. 139(3): p. 1268-83 [PMID:16258017] Ma S,Gong Q,Bohnert HJ Dissecting salt stress pathways. J. Exp. Bot., 2006. 57(5): p. 1097-107 [PMID:16510518] Tosti N, et al. Gene expression profiles of O3-treated Arabidopsis plants. Plant Cell Environ., 2006. 29(9): p. 1686-702 [PMID:16913859] Michel K,Abderhalden O,Bruggmann R,Dudler R Transcriptional changes in powdery mildew infected wheat and Arabidopsis leaves undergoing syringolin-triggered hypersensitive cell death at infection sites. Plant Mol. Biol., 2006. 62(4-5): p. 561-78 [PMID:16941219] Qutob D, et al. Phytotoxicity and innate immune responses induced by Nep1-like proteins. Plant Cell, 2006. 18(12): p. 3721-44 [PMID:17194768] Ko JH,Yang SH,Park AH,Lerouxel O,Han KH ANAC012, a member of the plant-specific NAC transcription factor family, negatively regulates xylary fiber development in Arabidopsis thaliana. Plant J., 2007. 50(6): p. 1035-48 [PMID:17565617] Brown BA,Jenkins GI UV-B signaling pathways with different fluence-rate response profiles are distinguished in mature Arabidopsis leaf tissue by requirement for UVR8, HY5, and HYH. Plant Physiol., 2008. 146(2): p. 576-88 [PMID:18055587] Scarpeci TE,Zanor MI,Carrillo N,Mueller-Roeber B,Valle EM Generation of superoxide anion in chloroplasts of Arabidopsis thaliana during active photosynthesis: a focus on rapidly induced genes. Plant Mol. Biol., 2008. 66(4): p. 361-78 [PMID:18158584] Gandia-Herrero F, et al. Detoxification of the explosive 2,4,6-trinitrotoluene in Arabidopsis: discovery of bifunctional O- and C-glucosyltransferases. Plant J., 2008. 56(6): p. 963-74 [PMID:18702669] Inz A subcellular localization compendium of hydrogen peroxide-induced proteins. Plant Cell Environ., 2012. 35(2): p. 308-20 [PMID:21443605] Causier B,Ashworth M,Guo W,Davies B The TOPLESS interactome: a framework for gene repression in Arabidopsis. Plant Physiol., 2012. 158(1): p. 423-38 [PMID:22065421] Besseau S,Li J,Palva ET WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in Arabidopsis thaliana. J. Exp. Bot., 2012. 63(7): p. 2667-79 [PMID:22268143] Koyama T, et al. A regulatory cascade involving class II ETHYLENE RESPONSE FACTOR transcriptional repressors operates in the progression of leaf senescence. Plant Physiol., 2013. 162(2): p. 991-1005 [PMID:23629833] Scarpeci TE,Zanor MI,Mueller-Roeber B,Valle EM Overexpression of AtWRKY30 enhances abiotic stress tolerance during early growth stages in Arabidopsis thaliana. Plant Mol. Biol., 2013. 83(3): p. 265-77 [PMID:23794142] Jiang W,Wu J,Zhang Y,Yin L,Lu J Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses. Protoplasma, 2015. 252(5): p. 1361-74 [PMID:25643917] Kanofsky K,Bahlmann AK,Hehl R,Dong DX Combinatorial requirement of W- and WT-boxes in microbe-associated molecular pattern-responsive synthetic promoters. Plant Cell Rep., 2017. 36(6): p. 971-986 [PMID:28341984]